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Full-Text Articles in Medicine and Health Sciences
Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Michael F. Mcentee, Steven C. Minkin, Seung J. Baek, John P. Biggerstaff, Jay Whelan
Zyflamend, A Polyherbal Mixture, Down Regulates Class I And Class Ii Histone Deacetylases And Increases P21 Levels In Castrate-Resistant Prostate Cancer Cells, E-Chu Huang, Yi Zhao, Guoxun Chen, Michael F. Mcentee, Steven C. Minkin, Seung J. Baek, John P. Biggerstaff, Jay Whelan
Seung J Baek
BACKGROUND: Zyflamend, a mixture containing extracts of ten herbs, has shown promise in a variety of preclinical cancer models, including prostate cancer. The current experiments were designed to investigate the effects of Zyflamend on the expression of class I and II histone deacetylases, a family of enzymes known to be over expressed in a variety of cancers. METHODS: CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. Involvement of p21 was confirmed with siRNA knockdown …
Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder
Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder
Ann Reed
Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated.
Objectives. The purposes of this prospective clinical study were to assess the precision of …
Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder
Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder
Karen M. Tobias DVM, DACVS, College of Veterinary Medicine
Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated. Objectives. The purposes of this prospective clinical study were to assess the precision of …
Tolfenamic Acid Induces Apoptosis And Growth Inhibition In Head And Neck Cancer: Involvement Of Nag-1 Expression, S. U. Kang, Y. S. Shin, H. S. Hwang, Seung J. Baek, S. Lee, C. Kim
Tolfenamic Acid Induces Apoptosis And Growth Inhibition In Head And Neck Cancer: Involvement Of Nag-1 Expression, S. U. Kang, Y. S. Shin, H. S. Hwang, Seung J. Baek, S. Lee, C. Kim
Seung J Baek
Nonsteroidal anti-inflammatory drug-activated gene-1 (NAG-1) is induced by nonsteroidal anti-inflammatory drugs and possesses proapoptotic and antitumorigenic activities. Although tolfenamic acid (TA) induces apoptosis in head and neck cancer cells, the relationship between NAG-1 and TA has not been determined. This study investigated the induction of apoptosis in head and neck cancer cells treated by TA and the role of NAG-1 expression in this induction. TA reduced head and neck cancer cell viability in a dose-dependent manner and induced apoptosis. The induced apoptosis was coincident with the expression of NAG-1. Overexpression of NAG-1 enhanced the apoptotic effect of TA, whereas suppression …
Protein C Activity In Dogs: Adaptation Of A Commercial Human Colorimetric Assay And Evaluation Of Effects Of Storage Time And Temperature, Michael M. Fry, Karl R. Snyder, Karen M. Tobias, Baye G. Williamson, Ann Reed
Protein C Activity In Dogs: Adaptation Of A Commercial Human Colorimetric Assay And Evaluation Of Effects Of Storage Time And Temperature, Michael M. Fry, Karl R. Snyder, Karen M. Tobias, Baye G. Williamson, Ann Reed
Michael M Fry
Objectives of this study were to adapt a commercial human protein C (PC) colorimetric assay for use in dogs and to investigate effects of various storage conditions. The human assay was modified by using pooled canine plasma for calibration and by increasing the activation time. PC activity was measured in fresh canine plasma and in plasma stored under various conditions. PC activity of some stored samples was significantly different from that of fresh plasma; however, differences were small. No difference was detected in samples stored under similar conditions but analyzed in different laboratories using similar methodology. Results of this study …