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Chulalongkorn University

Infectious bronchitis virus

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Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu Mar 2019

Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu

The Thai Journal of Veterinary Medicine

The purpose of this study was to evaluate the effect of a rapid heat-treatment method on Thai QX-like infectious bronchitis virus (IBV) and the efficacy on protection from the virus after heat treatment. QX-like IBV isolate THA46 was incubated at 56 oC. The incubated virus was collected every 5 min for up to 1 h and then they were inoculated into embryonated chicken eggs. At 6 days post-inoculation, allantoic fluid was harvested from the embryonated eggs which were inoculated with virus of the longest heat-treatment time that induced lesions in the embryos. That allantoic fluid was used in a subsequent …


Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan Sep 2015

Efficacy Of Live H120 Strain Against Variant Genotype Infectious Bronchitis Virus Isolated In Thailand In Broiler Chickens, Tawatchai Pohuang, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study investigated the level of protection generated by vaccination with a live attenuated infectious bronchitis virus (IBV) vaccine, H120 strain against variant genotype IBV isolated in Thailand. Sixty 1-day-old female broiler chickens were randomly divided into 4 groups of 15 chickens each. The chickens in Group 1 were vaccinated at 1 day old, whereas the chickens in Group 2 were vaccinated at 7 days old. Groups 3 and 4 did not receive IBV vaccine and served as positive and negative control groups, respectively. At 21 days of age, the chickens in Groups 1-3 were individually challenged with 104.8 EID50 …


Efficacy Of Live Infectious Bronchitis Vaccine Programs Against Infection By Qx-Like Strain Of Infectious Bronchitis Virus, Ekasingh Sarueng, Wisanu Wanasawaeng, Jiroj Sasipreeyajan, Niwat Chansiripornchai Jun 2014

Efficacy Of Live Infectious Bronchitis Vaccine Programs Against Infection By Qx-Like Strain Of Infectious Bronchitis Virus, Ekasingh Sarueng, Wisanu Wanasawaeng, Jiroj Sasipreeyajan, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The aim of this study was to evaluate the efficacy of live infectious bronchitis (IB) vaccine programs against infection by a QX-like strain of the IB virus isolated in Thailand. The experimental groups were divided into 5 groups. Group 1 broilers were administered with Poulvac IB QX-like at 1 day old. Group 2 broilers were administered with Poulvac IB QX-like at 1 and 14 days old. Group 3 broilers were administered with Nobilis IB H120 and Poulvac IB QX-like at 1 and 14 days old, respectively. Group 4 broilers were administered with Nobilis IB H120 and Nobilis IB 4-91 at …


Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai Sep 2013

Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study aimed to perform an in vitro testing of the efficacy of various kinds of disinfectant on infectious bronchitis virus (IBV). Four kinds of disinfectants including Virusnip, Omnicide, CID2000 and Virkon S were used for the virus inactivation test. After challenge with each strain of IBV for each contact time (30 seconds, 1, 5 and 30 minutes), we recorded number of dead embryo/6 inoculated eggs after incubation. Results showed that there was no significant difference among the contact times except 1:800 Virusnip tested for Tha07 (p < 0.05). For Tha03, there was a significant difference among the disinfectants at 30 seconds and 1 minute of contact times (p < 0.05). For Tha08, there was a significant difference among the disinfectants at 1 minute of contact time (p < 0.05). For Tha09, there was a significant difference among the disinfectants at 5 and 30 minutes of contact times (p < 0.05). For Tha10, there was a significant difference among the disinfectants at 5 minutes of contact time (p < 0.05). Virusnip revealed the ability to inactivate the activity of 9 IBV strains in all exposure times, especially at dilutions of 1 : 100 and 1 : 200.


The Pathogenesis Of A New Variant Genotype And Qx-Like Infectious Bronchitis Virus Isolated From Chickens In Thailand, Tawatchai Pohuang, Jiroj Sasipreeyajan Mar 2012

The Pathogenesis Of A New Variant Genotype And Qx-Like Infectious Bronchitis Virus Isolated From Chickens In Thailand, Tawatchai Pohuang, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study was designed to investigate the pathogenesis of infectious bronchitis virus (IBV), a new variantgenotype and QX-like IBV, isolated in Thailand. Sixty, 28-day-old, female broiler chickens were divided into 3 groupsof 20 chickens each (groups 1-3) and sixty, 1-day-old, female broiler chickens were divided into 3 groups of 20chickens each (groups 4-6). The chickens in groups 2 and 5 were inoculated with QX-like IBV (isolate THA80151)whereas the chickens in groups 3 and 6 were inoculated with a new variant genotype IBV (isolate THA90151). Thechickens in groups 1 and 4 served as negative control groups. Clinical signs and mortality rates …


Efficacy Of Different Vaccination Programs Against Thai Qx-Like Infectious Bronchitis Virus, Jiroj Sasipreeyajan, Tawatchai Pohuang, Nida Sirikobkul Mar 2012

Efficacy Of Different Vaccination Programs Against Thai Qx-Like Infectious Bronchitis Virus, Jiroj Sasipreeyajan, Tawatchai Pohuang, Nida Sirikobkul

The Thai Journal of Veterinary Medicine

The present study was designed to evaluate the protection induced by different vaccination programs against Thai QX-like infectious bronchitis virus (IBV). The chickens were divided into 5 groups, 16 chickens in each. Groups 1-4 were vaccinated with different regimes of vaccination program against IBV. Groups 5 did not receive IBV vaccine and served as a positive control group. At 1 day old, the chickens in groups 2 and 3 were vaccinated with QX vaccine whereas the chickens in group 4 were vaccinated with H120 vaccine. At 14 days old, revaccination was performed in group 2 with QX vaccine whereas groups …


Antibody Titres In Broilers Of Infectious Bronchitis Virus Strains 4/91 And Massachusetts In Thailand, Thawat Lekdumrongsak, Jiroj Sasipreeyajan, Prachak Poomvises, Achara Tawatsin Dec 2001

Antibody Titres In Broilers Of Infectious Bronchitis Virus Strains 4/91 And Massachusetts In Thailand, Thawat Lekdumrongsak, Jiroj Sasipreeyajan, Prachak Poomvises, Achara Tawatsin

The Thai Journal of Veterinary Medicine

Thirty, one-day-old broilers were divided into 3 groups, 10 birds each. Group 1 was vaccinated twice with a live infectious bronchitis virus vaccine, (IBV)-serotype Massachusetts, strain H120 when one day old and a live IBV-strain Massachusetts vaccine when ten days old. Group 2 was vaccinated once with a live IBV-strain Massachusetts vaccine when ten days old. Group 3 were non-vaccinated controls. Blood samples were taken from birds on days 1, 10, 21, 28, 35 and 42. Samples were tested by a virus neutralization(VN) test, for strains 4/91 and Massachusetts(M41) neutralizing antibodies and were tested by an enzyme-linked immunosorbent assay(ELISA) for …