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Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu Dec 2004

Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the ultrastructural changes seen in frozen porcine oocytes at the germinal vesicle and during in vitro maturation when using an ultrarapid freezing technique. The morphological changes were assessed by transmission electron microscopy (TEM). Immature oocytes were collected from pig ovaries at a local slaughter house in Nakorn Prathom province. Some oocytes were matured in vitro for 44 hours at 39oC, 5% CO2 with high humidity. All oocytes were divided into four groups; immature (control group), the in vitro maturation group (control group), frozen immature and frozen mature oocytes. Electron microscopy was …


Fatal Foreign Body Pulmonary Embolism And Granulomatosisin Intravenous Drug Addict: A Case Report, M. Taweevisit, U. Phumsuk, N. Upariwong Dec 2004

Fatal Foreign Body Pulmonary Embolism And Granulomatosisin Intravenous Drug Addict: A Case Report, M. Taweevisit, U. Phumsuk, N. Upariwong

Chulalongkorn Medical Journal

No abstract provided.


Melan A, Protein Expression As A Diagnostic And Prognostic Factor In Canine Oral Melanomas, Anudep Rungsipipat, Chanin Kulprawit Dec 2004

Melan A, Protein Expression As A Diagnostic And Prognostic Factor In Canine Oral Melanomas, Anudep Rungsipipat, Chanin Kulprawit

The Thai Journal of Veterinary Medicine

The purpose of this paper is to evaluate the relationship between Melan A protein expression, histopathological appearance, clinical data and proliferative marker indices for the diagnosis and prognosis of canine oral melanomas. A retrospective study was performed on biopsy specimens of 65 dogs submitted during the years of 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results revealed that Melan A protein expression was detected in 50.77% (33/65) malignant melanomas. The results did not show any significant differences between Melan A protein expression and histopathological classification, clinical data, average AgNORs and PCNA indices. …


The Prevalence And Treatment Of Eyelid Neoplasms In Dogs : 69 Cases (2000-2003), Nalinee Tuntivanich, Pranee Tuntivanich, Santi Wongaumnuaykul, Anudep Rungsipipat Dec 2004

The Prevalence And Treatment Of Eyelid Neoplasms In Dogs : 69 Cases (2000-2003), Nalinee Tuntivanich, Pranee Tuntivanich, Santi Wongaumnuaykul, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department of Surgery, Small Animal Hospital, Chulalongkorn University, between November 2000 and November 2003, in relation to breed, sex, age, location, tumor size, histopathological diagnosis and surgical treatment is reported. The average age of the affected dogs was 7.85 years. Mixed breeds, males and the upper eyelid were at a higher risk. The average size of the mass was 6.49 mm. Benign tumors (88.41%) were more frequent than malignant ones (11.59%). Sebaceous gland tumors were more frequently encounted (79.7%) than malignant tumors, malignant melanomas, squamous cell …


Ultrasound Diagnosis, Phiwipha Kamonrat Dec 2004

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai Dec 2004

Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai

The Thai Journal of Veterinary Medicine

Three hundred and sixty day-old chicks were used in this study to investigate the effects of organic acids on the control of Salmonella. The chicks were divided into uninoculated and inoculated groups, 180 in each. Both groups were subdivided into 3 further groups of 60 chicks each. The uninoculated, control group comprised of, group 1 : given diluted organic acids 1:1000 via the drinking water, group 2 : given organic acids diluted at 1:2000 and group 3 : given water alone. Each bird in the three inoculated subgroups 4, 5 and 6 were orally dosed with 0.3 ml of brain …


Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Dec 2004

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.


The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat Dec 2004

The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade II, 41.46% but there was no statistically significant difference between them. An evaluation of a proliferative marker shown by a mean (±SD) of PCNA, was 34.63±23.87 and the mean (±SD) of AgNORs was 1.23±0.18. There was no significant difference between the PCNA and AgNORs index of both the histological types and the histological malignancy grades. However, a statistical difference of mean PCNA and AgNORs indices was noted in both c-erbB2 positive and c-erbB2 negative groups. The mean PCNA indices and AgNORs indices in c-erbB2 positive samples were 26.52±18.68 and 1.12±0.27 respectively, while the c-erbB2 negative ones were 39.43±26.24 and 1.28±0.19 respectively. In conclusion, c-erbB2 oncogene protein expression is considered to be an effective prognostic factor for adenocarcinoma subtypes of feline mammary tumors as it is in human and canine cases. However, integration of all the parameters (c-erbB2 oncogene protein, PCNA index, AgNORs index, histological types and clinical data) may achieve a more precise prognosis in feline, mammary tumor cases.


The Relationship Between Agnors And Pcna Indices, Histopathological Appearance And Clinical Factors, As Prognostic Factor For Canine Oral Melanoma, Anudep Rungsipipat, Siriwalee Ngow, Nipaporn Pattanathaiyanom, Nipaporn Pattanathaiyanom, Porntipa Lertrattanachaikul, Khanchit Pongpech, Chanin Kulprawit Dec 2004

The Relationship Between Agnors And Pcna Indices, Histopathological Appearance And Clinical Factors, As Prognostic Factor For Canine Oral Melanoma, Anudep Rungsipipat, Siriwalee Ngow, Nipaporn Pattanathaiyanom, Nipaporn Pattanathaiyanom, Porntipa Lertrattanachaikul, Khanchit Pongpech, Chanin Kulprawit

The Thai Journal of Veterinary Medicine

The purpose of this research was to evaluate the relationship between histopathological appearance and clinical data, Argyrophilic Nucleolar Organizer Regions (AgNORs) and Proliferative Cell Nuclear Antigen (PCNA) indices in canine oral melanoma. A retrospective study was performed on biopsy specimens from 67 dogs submitted over the year 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results showed that the average AgNORs index did not show any significant differences with histopathological characteristics and Masson-Fontana Silver (MFS) stain but the AgNORs index showed significant differences when compared with histological grading and tumor diameter size (p < 0.05). The average PCNA index did not show significant differences with histopathological characteristics, grading, and MFS stain but did not show significant differences with the sex of affected dogs and tumor diameter size (p < 0.05). AgNORs and PCNA indices might be useful as a prognostic factor for oral melanoma in dogs


In Vitro Effects Of Sodium Hypochlorite, Trisodium Phosphate And Organic Acids On The Decontamination Of Salmonella Enterica Serovar Enteritidis On Chicken Skin, Rungtip Chuanchuen, Chailai Koowatananukul, Valasinee Rugkhaw, Thanis Damrongwatanapokin Dec 2004

In Vitro Effects Of Sodium Hypochlorite, Trisodium Phosphate And Organic Acids On The Decontamination Of Salmonella Enterica Serovar Enteritidis On Chicken Skin, Rungtip Chuanchuen, Chailai Koowatananukul, Valasinee Rugkhaw, Thanis Damrongwatanapokin

The Thai Journal of Veterinary Medicine

This study was conducted to evaluate the effectiveness of five chemical disinfectants, including sodium hypochlorite (CL), trisodium phosphate (TSP) and organic acids; acetic acid (AA), citric acid (CA) and lactic acid (LA), in reducing of the contamination level of Salmonella enterica Serovar Enteritidis (S. Enteritidis) on chicken skin. Chicken drumsticks were artificially contaminated with the kanamycin resistant S. Enteritidis (SEnKmr) to obtain 105-106 CFU/cm2. The five disinfectants used were: CL; 25, 50 and 100 ppm, TSP; 4, 8 and 12%, AA, LA and CA; 0.6, 1.2 and 2.4%. The disinfectants were applied by either dipping for 1, 3 and 5 …


Clinicopathological Study Of Testicular Tumors Diagnosedat King Chulalongkorn Memorial Hospital Betweenjanuary 1999 And July 2004, N. Chirakalwaasan Dec 2004

Clinicopathological Study Of Testicular Tumors Diagnosedat King Chulalongkorn Memorial Hospital Betweenjanuary 1999 And July 2004, N. Chirakalwaasan

Chulalongkorn Medical Journal

No abstract provided.


A Comparative Study On The Isolation Of Ureaplasma Diversum From The Vulvae Of The Cattle, Using Three Different Culture Media, Junpen Suwimonteerabutr, Prachin Virakul Dec 2004

A Comparative Study On The Isolation Of Ureaplasma Diversum From The Vulvae Of The Cattle, Using Three Different Culture Media, Junpen Suwimonteerabutr, Prachin Virakul

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the optimum culture media for Ureaplasma diversum. The study used 3 culture media, TR broth and differential A 7 agar (1), Boston broth and differential A7 agar (2) and differential A7 agar (3). The samples were collected from the vulvae of 15 cows that had a reddish, mucosal, vulva lesion and 15 cows without the lesion. A liquid culture media was prepared using the serial, tenfold, dilutionmethod to avoid contamination from other microflora. The type of U. diversum was determined using A7 agar mixed with manganese sulfate. Positive results were observed …


Multidrug Efflux Systems (Mes): A Mechanism Of Resistance To Multidrug In Bacteria, Rungtip Chuanchuen, Herbert P. Schweizer Dec 2004

Multidrug Efflux Systems (Mes): A Mechanism Of Resistance To Multidrug In Bacteria, Rungtip Chuanchuen, Herbert P. Schweizer

The Thai Journal of Veterinary Medicine

Bacterial antibiotic resistance is a growing problem with a global dimension. While the mechanisms of resistance vary from agent to agent, most of them are agent specific, providing resistance to a single or a class of anitimicrobials. Multidrug efflux systems have currently emerged as a predominant cause of antimicrobial resistance across a broad spectrum of structurally - unrelated antimicrobials. Efflux-mediated resistance is promoted by the exclusion of agents from the systems, resulting in reduced intracellular concentration of drugs. MES occur in many Gram-positive and Gram-negative bacteria, including medically important pathogens, therefore they are of significant concern. Expression of the systems …


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph Dec 2004

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


The Results Of Using Pumices As A Bedding In Broiler Chicken House, Somsak Pakpinyo, Boonrit Thongsong, Thawat Lekdumrongsak, Somporn Wangsoongnoen, Suthep Ruangwises Dec 2004

The Results Of Using Pumices As A Bedding In Broiler Chicken House, Somsak Pakpinyo, Boonrit Thongsong, Thawat Lekdumrongsak, Somporn Wangsoongnoen, Suthep Ruangwises

The Thai Journal of Veterinary Medicine

The objective of this experiment was to compare the rice hull and pumice litter when used as bedding in a broiler chicken house. Seven hundred and fourteen 1-day-old, broiler chicks were divided equally into 2 groups. Group 1 raised on rice hulls and group 2 on pumice litter. Each group had 3 replicates, 119 birds in each. The average body weight gain and the feed intake during days 1-42 were observed in order to calculate the feed conversion rate (FCR). At 16, 28, and 42 days, 10 birds from each replicate were bled for their antibody titer responses to Newcastle …


The Characterization Of Clays And Cetylpyridiniumexchanged Clays For Their Ability To Adsorb Zearalenone, Komkrich Pimpukdee, Bundit Tengjaroenkul, Prapansak Chaveerach, Benjamas Mhosatanun Dec 2004

The Characterization Of Clays And Cetylpyridiniumexchanged Clays For Their Ability To Adsorb Zearalenone, Komkrich Pimpukdee, Bundit Tengjaroenkul, Prapansak Chaveerach, Benjamas Mhosatanun

The Thai Journal of Veterinary Medicine

The adsorption of zearalenone (ZEN) onto Ca-montmorillonite (STx-1), Na-montmorillonite (SWy-2), acid-activated montmorillonite (AAM), clinoptilolite and cetylpyridinium (CP) exchanged clays was studied. The results indicated that CP-exchanged clays were more effective than unmodified base clays in adsorbing ZEN, i. e. STx-1 (7.4±2.3 mg/g) < clinoptilolite (7.6±1.1 mg/g) < AAM (10.0±0.0 mg/g) < SWy-2 (11.9±0.4 mg/g), whereas the extent of ZEN adsorption into modified clay products did not follow the order seen in the based clays. The adsorption efficiency of CP-exchanged clays is in the order of CP*STx-1 (56.9±1.1 mg/g) = CP*AAM (56.9±1.8 mg/g) > CP*SWy-2 (47.9±2.6 mg/g) > CP*clinoptilolite (15.5±1.1 mg/g). Three CP-exchanged clays were further characterized by isotherm studies. The results indicated that the isotherm shapes of CP*exchanged clays exhibited a S-shape. The isotherm plot is concave up to the inflection point and then plateaus, which suggests multiple, specific binding sites and saturation at each site. It is important to rigorously test …


Ki-67 Labeling Index, Hormonal Status, And Invasivenessof Pituitary Adenomas: A Clinicopathological Study, S. Chiewchankoljuk, S. Lalitanantpong, M. Chipipat, V. Wattanasirmkit, S. Shuangshoti Dec 2004

Ki-67 Labeling Index, Hormonal Status, And Invasivenessof Pituitary Adenomas: A Clinicopathological Study, S. Chiewchankoljuk, S. Lalitanantpong, M. Chipipat, V. Wattanasirmkit, S. Shuangshoti

Chulalongkorn Medical Journal

No abstract provided.


Renal Cell Carcinoma With Sarcomatoid Andrhabdoid Feature, W. Kittikowit, V. Thanakit Dec 2004

Renal Cell Carcinoma With Sarcomatoid Andrhabdoid Feature, W. Kittikowit, V. Thanakit

Chulalongkorn Medical Journal

No abstract provided.


Update In Diagnosis Of Renal Glomerular Diseases, W. Kittikowit Dec 2004

Update In Diagnosis Of Renal Glomerular Diseases, W. Kittikowit

Chulalongkorn Medical Journal

No abstract provided.


Retrospective Study Of Acute Cellular Rejection Intransplanted Liver In Pathology Unit In Kingchulalongkorn Memorial Hospital Duringthe Year 1997 To 2003 : 11 Studied Cases, D. Thirabanjasak, N. Wisedopas, L. Sansopha Dec 2004

Retrospective Study Of Acute Cellular Rejection Intransplanted Liver In Pathology Unit In Kingchulalongkorn Memorial Hospital Duringthe Year 1997 To 2003 : 11 Studied Cases, D. Thirabanjasak, N. Wisedopas, L. Sansopha

Chulalongkorn Medical Journal

No abstract provided.


Doctor And Forensic Medicine, N. Tansrisaward Nov 2004

Doctor And Forensic Medicine, N. Tansrisaward

Chulalongkorn Medical Journal

No abstract provided.


Prevalence Of Autopsied Bodies That Are Anti-Hiv Positive,Distributed According To Various Basic Factorsidentified At Chulalongkorn Forensic Center, K. Vongpaisarnsin Nov 2004

Prevalence Of Autopsied Bodies That Are Anti-Hiv Positive,Distributed According To Various Basic Factorsidentified At Chulalongkorn Forensic Center, K. Vongpaisarnsin

Chulalongkorn Medical Journal

No abstract provided.


Database Of Autopsy In Forensic Medicine Department Of Khonkaen University From 1998 To 2002, S. Boonluk Nov 2004

Database Of Autopsy In Forensic Medicine Department Of Khonkaen University From 1998 To 2002, S. Boonluk

Chulalongkorn Medical Journal

No abstract provided.


The Right Answer To The Wrong Question, N. Tansrisaward Nov 2004

The Right Answer To The Wrong Question, N. Tansrisaward

Chulalongkorn Medical Journal

No abstract provided.


Health Belief Model Teaching Program Forthalassemia Education In High School Students, S. Lagampan, P. Lapvongwatana, C. Tharapan, J. Nonthikorn Nov 2004

Health Belief Model Teaching Program Forthalassemia Education In High School Students, S. Lagampan, P. Lapvongwatana, C. Tharapan, J. Nonthikorn

Chulalongkorn Medical Journal

No abstract provided.


Bilateral Giant Adrenal Myelolipomas In A Young Adult, V. Poklang, M. Taweevisit, U. Phymsuk, W. Kittikowit, Sh. Shuagshoti Nov 2004

Bilateral Giant Adrenal Myelolipomas In A Young Adult, V. Poklang, M. Taweevisit, U. Phymsuk, W. Kittikowit, Sh. Shuagshoti

Chulalongkorn Medical Journal

No abstract provided.


Diagnosis Of The Cushing's Disease And Definition Of Cure After Transphenoidal Surgery, S. Chungsamarn, S. Sunthornyothin Nov 2004

Diagnosis Of The Cushing's Disease And Definition Of Cure After Transphenoidal Surgery, S. Chungsamarn, S. Sunthornyothin

Chulalongkorn Medical Journal

No abstract provided.


Amblyopia Treatment Reevaluated, Wasee Tulvatana Oct 2004

Amblyopia Treatment Reevaluated, Wasee Tulvatana

Chulalongkorn Medical Journal

No abstract provided.


The Right Answer To The Wrong Question, Kittisak Kulvichit Oct 2004

The Right Answer To The Wrong Question, Kittisak Kulvichit

Chulalongkorn Medical Journal

No abstract provided.


Treatment Of Asymptomatic Primary Angle Closure, Visanee Tantisevi Oct 2004

Treatment Of Asymptomatic Primary Angle Closure, Visanee Tantisevi

Chulalongkorn Medical Journal

No abstract provided.