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A Simple And Efficient Dna Extraction From Respiratory Samples For Pcr Detection Of Pneumocystis Jirovecii, Phairote Teeranaipong, Taweesak Tia, Chaturong Putaporntip

Chulalongkorn Medical Journal

Background : Pneumonia caused by Pneumocystis jirovecii (PCP) is a leading cause ofmorbidity and mortality in immunocompromised individuals. Althoughmicroscopic detection of stained clinical samples and immunofluorescenceassay (IFA) have been widely used for diagnosis of P. jirovecii infections,polymerase chain reaction (PCR)-based detection of P. jirovecii provides ahigher diagnostic performance. DNA preparation by simple boiling methodcould be useful for PCR detection albeit the sensitivity remained remarkablylow.Objective : To refine P. jirovecii DNA preparation by boiling method in order to improvethe diagnostic yield of subsequent PCR detection.Methods : Two induced sputum and 4 bronchoalveolar lavage (BAL) samples fromPCP patients were recruited for initial …