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Expression Of Recombinant Major Surface Protein 5 Of Anaplasma Marginale (A. Marginale) At Different Temperatures, Amaya Watthanadirek, Thunyaporn Doungdaolek, Preeyaporn Koedrith, Panat Anuracpreeda
Expression Of Recombinant Major Surface Protein 5 Of Anaplasma Marginale (A. Marginale) At Different Temperatures, Amaya Watthanadirek, Thunyaporn Doungdaolek, Preeyaporn Koedrith, Panat Anuracpreeda
Chulalongkorn Medical Journal
Background : Bovine anaplasmosis is an important tick-borne disease caused byAnaplasma marginale (A. marginale) and infected in ruminants, mostly incattle. This disease occurs in tropical and subtropical regions includingThailand and causes a major problem to livestock productions. The majorsurface protein 5 (MSP5) is one of outer membrane protein of A. marginalewhich as an immunodominant protein encoded by a single gene and alsohighly conserved gene.Objective : The aim of this study was to optimize the conditions for the expression ofrecombinant major surface protein 5 (rMSP5) of A. marginale.Methods : The msp5 gene of A. marginale was cloned into the pET100/D-TOPO®vector to …