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Articles 91 - 119 of 119
Full-Text Articles in Medicine and Health Sciences
The Molecular Mechanisms Underlying Cardiac Hypertrophy Due To Volume Overload., Justin Andrew Barnes
The Molecular Mechanisms Underlying Cardiac Hypertrophy Due To Volume Overload., Justin Andrew Barnes
All ETDs from UAB
Mitral regurgitation (MR) is a common form of heart disease, but there are no approved medical therapies for MR or other cardiovascular conditions associated with hemodynamic volume overload (VO) and eccentric remodeling. We have previously shown that a standard heart failure treatment, ß-adrenergic system blockade (ß-RB), improves function in animal models of VO and in MR patients without attenuating the adverse eccentric hypertrophy that occurs in conjunction with VO. This paradox highlights how poorly we understand eccentric remodeling of the heart, and a better understanding of the molecular mechanisms at work in VO must be obtained before we can develop …
The Relationship Of Maternal Depressive Symptoms Among First Time Adolescent And Adult Mothers And Their Safe Parenting Practices And Child Injuries, Angela Warren Baumann
The Relationship Of Maternal Depressive Symptoms Among First Time Adolescent And Adult Mothers And Their Safe Parenting Practices And Child Injuries, Angela Warren Baumann
All ETDs from UAB
Maternal depressive symptoms have been associated with deficits in parenting practices that protect children from injuries; however, few studies have been conducted to examine these relationships. Using data from a multisite longitudinal study of 252 first-time mothers, the relationships between the use of safe parenting practices and maternal depressive symptoms was examined, taking into account mother's resources (as defined by age and education level) and race at five time points during the first 3 years of the child's life. Depressive symptoms were assessed using the Beck Depression Inventory. Measures of maternal depressive symptoms, safe parenting practices (use of car seat, …
Adapter Based Strategies For Adenovirus Vector Retargeting To T Lymphocytes, Matthew S. Beatty
Adapter Based Strategies For Adenovirus Vector Retargeting To T Lymphocytes, Matthew S. Beatty
All ETDs from UAB
Adenoviruses are the most commonly used gene therapy vector for cancer therapy clinical trials. While adenovirus has shown a great track record in a variety of cancer therapeutics it has not progressed as a vector system for the modification of T lymphocytes. One of the major roadblocks towards utilizing adenovirus for T cell therapy is the lack of coxsackie virus and adenovirus receptor on the cell surface of T cell lineages. Exploitation of alternative receptors has allowed adenovirus vectors to be utilized in a variety of cell types that native adenovirus type 5 cannot infect. Thus, retargeting adenovirus to an …
High-Tech Services And Hospital Performance In The United States, Ferhat Devrim Zengul
High-Tech Services And Hospital Performance In The United States, Ferhat Devrim Zengul
All ETDs from UAB
This dissertation examined the relationship between high-technology medical services and hospital performance by focusing on financial and quality performance dimensions. An initial systematic review disclosed the paucity of research and mixed findings about the technology-performance relationship. The review indicated the need for further analyses by emphasizing some limitations of existing studies. The resource-based view (RBV) of a firm was used as a conceptual framework in examining the relationship between high-tech medical services and hospital financial performance. It was hypothesized that large breadth (number) of high-tech services and the use of rare high-tech services would be positively associated with hospitals' financial …
Regulation Of Stim1 By O-Glcnacylation In Cardiomyocytes, Xiaoyuan Zhu-Mauldin
Regulation Of Stim1 By O-Glcnacylation In Cardiomyocytes, Xiaoyuan Zhu-Mauldin
All ETDs from UAB
In non-excitable cells, STIM1 and Orai1 are the primary mediators of store-operated Ca2+ entry (SOCE); however little is known about their roles in cardiomyocyte Ca2+ handling. We have previously shown that acute increases in the levels of O-GlcNAcylation (the attachment of O-linked N- acetylglucosamine (O-GlcNAc) to target proteins) are cardioprotective through attenuating Ca2+-mediated injury and increased O-GlcNAcylation blunts SOCE in cardiomyocytes. Therefore, the goals here were to determine the regulation of STIM1 and STIM1-mediated SOCE by O-GlcNAcylation in cardiomyocytes. Immunohistochemical analysis demonstrated the co-localization of STIM1 with SERCA and RyR in adult cardiomyocytes. NRVMs were transfected with the eYFP-tagged STIM1 …
Glioma-Vasculature Interactions, Stacey Watkins
Glioma-Vasculature Interactions, Stacey Watkins
All ETDs from UAB
Malignant gliomas are highly invasive brain tumors, which lack effective treatment options. To date, the standard therapy includes surgical debulking followed by chemotherapy and radiation. Despite efforts to eradicate the disease, the median patient survival time is only 14 months. In an attempt to discover novel therapies, research has focused on understanding the heterogeneous biological mechanisms employed by these cells. Uniquely, they do not metastasize through the vasculature or lymphatics like other solid cancers but alternatively, glioma cells utilize the abluminal surface of the vasculature to guide invasion and migration throughout the brain. The intimate association between glioma cells and …
The Relationship Of Religiosity, Spirituality And High-Risk, Rev Tommie Lee Watkins Jr
The Relationship Of Religiosity, Spirituality And High-Risk, Rev Tommie Lee Watkins Jr
All ETDs from UAB
Religion and spirituality are important concepts for many Black MSM. While many social factors may be associated with the increasing disparity of HIV infection among Black MSM, high levels of religiosity and spirituality prevalent in Black culture may be correlated with high risk behavior among Black MSM and may help explain this population's increasing incidence of HIV infection. Research supports a relationship between religiosity, spirituality, and high risk behavior among populations. Some research has suggested that religiosity and spirituality among Black MSM may be correlated but are multifaceted constructs. Conclusions suggest that among Black MSM these constructs account for certain …
Hur Regulation In Central Nervous System Disorders, Crystal G. Wheeler
Hur Regulation In Central Nervous System Disorders, Crystal G. Wheeler
All ETDs from UAB
In inflammatory diseases of the central nervous system (CNS), astrocytes play a major role in the systemic response to disease by either enhancing or limiting the inflammatory response through secretion of growth factors or cytokines. HuR RNA binding protein regulates many genes involved in inflammation. Inflammatory diseases such as brain tumors, Amylotrophic Lateral Sclerosis (ALS), and Multiple Sclerosis (MS), have been linked to HuR, by our laboratory and others. To determine whether astrocytic overexpression of HuR would regulate inflammation in diseases of the CNS, we designed a transgenic mouse in which the HuR gene is overexpressed. In the first study, …
Anti-Group B Streptococci Monoclonal Antibodies Protect Against Invasive Aspergillosis, Rebekah Wharton
Anti-Group B Streptococci Monoclonal Antibodies Protect Against Invasive Aspergillosis, Rebekah Wharton
All ETDs from UAB
Aspergillus fumigatus is a ubiquitous saprophytic fungus which can potentially cause life-threatening infections in immunocompromised individuals. Although a serious threat, current treatments are largely ineffective at reducing morbidity and mortality. To this end, we have characterized a monoclonal antibody generated against Group B Streptococci, type Ib, which also recognizes Aspergillus fumigatus conidia and hyphae. We show that this antibody appears to bind a similar epitope on both pathogens and provides a significant degree of protection in a murine model of invasive aspergillosis via a mechanism that appears to be dependent on complement. The studies we performed using this antibody should …
Commensal Microbiota And Its Impact On Health And Autoimmune Diabetets, Kyle James Wolf
Commensal Microbiota And Its Impact On Health And Autoimmune Diabetets, Kyle James Wolf
All ETDs from UAB
Environmental exposures such as diet, use of antibiotics, and lifestyle have significant impact on the health of an individual. One mechanism that acts as a major pathway in these environmental exposures is the role intestinal commensal microbiota play in both metabolic and immunological responses. Metabolic pathways are altered through changes in metabolites provided by commensal microbiota; alterations in microbial make-up can have drastic impacts on metabolic function. Similarly, the development and maturation of the immune system is dependent on the intestinal microbes to induce tolerance and act as both an immune modulator as well as a barrier against pathogens. We …
The Effects Of Nicotine On The Human Adult Visual Pathway And Processing, Stefanie B. Varghese
The Effects Of Nicotine On The Human Adult Visual Pathway And Processing, Stefanie B. Varghese
All ETDs from UAB
Nicotine, the primary addictive component found in tobacco/cigarettes, can bind to nicotinic acetylcholine receptors (nAChR), which are found throughout the central nervous system including the retina of the eye. Previous studies have shown nAChR expression in the retina of non-human and human (Hutchins & Hollyfield, 1985; Liu et al., 2009). By use of electrophysiologic measures, i.e. electroretinograms (ERGs), early reports indicated that nicotine and/or cigarette smoking affects vision and visual processing in different species (Jurklies, Kaelin-Lang, & Niemeyer, 1996; Jünemann & Damaske, 1968). Because previous human studies have used only cigarette smokers, there are questions about what role nicotine, itself, …
Intrinsic Properties Of Rna Polymerase I And Trans-Acting Factors Control Transcription Elongation Efficiency, Olga V. Viktorovskaya
Intrinsic Properties Of Rna Polymerase I And Trans-Acting Factors Control Transcription Elongation Efficiency, Olga V. Viktorovskaya
All ETDs from UAB
Transcription of ribosomal (r) DNA by RNA polymerase I (Pol I) is the initial step of ribosome synthesis. Pol I transcription is unique in its high rate of initiation, specific organization within the nucleolus and tight connection to cell growth and proliferation. Moreover, transcription elongation by Pol I is functionally coupled with rRNA processing and assembly of the ribosomes. Regulatory insights into transcription elongation by Pol I and its interface with rRNA processing are limited, despite decades of research. To fulfill that gap, we asked several important questions: Do the obvious functional divergences between Pol I and other eukaryotic polymerases …
Association Between Cruciferous Vegetable Intake And Risk Of Colorectal Cancer Among Men In Shanghai, China, Emily Vogtmann
Association Between Cruciferous Vegetable Intake And Risk Of Colorectal Cancer Among Men In Shanghai, China, Emily Vogtmann
All ETDs from UAB
Colorectal cancer (CRC) is a major global health concern and the intake of certain foods, such as cruciferous vegetables, has been studied for their potential protective effects against cancer development. The observed association between cruciferous vegetable consumption and CRC has been inconsistent, possibly related to glutathione S-transferase (GST) gene polymorphisms. Therefore, we aimed to (1) evaluate the association between fruits and vegetables, including cruciferous vegetables, on the risk of CRC; (2) determine factors associated with urinary isothiocyanate (ITC), a biomarker of cruciferous vegetable intake; and (3) evaluate the association between cruciferous vegetable consumption and CRC and to estimate the potential …
Gender Norms, Sexual And Reproductive Behaviors And Acceptability Of Male Circumcision Among Men In Western Jamaica, Melonie Walcott
Gender Norms, Sexual And Reproductive Behaviors And Acceptability Of Male Circumcision Among Men In Western Jamaica, Melonie Walcott
All ETDs from UAB
This dissertation consists of three papers designed to assess the association between gender norms and men's sexual behaviors, reproductive practices, and acceptability of male circumcision (MC). The data for this study were obtained from a cross-sectional, interviewer-administered questionnaire survey of 549 men aged 19-54 years, conducted in the western region of Jamaica between June to August 2011. In the first paper we found increased odds of reporting multiple sex partners among men with moderate (AOR =2.2; 95% CI=1.4 - 3.5) and high (AOR = 4.4; 95% CI =2.2 - 8.8) support for inequitable gender norms. Similarly, men with moderate (AOR= …
The Impact Of Transcription And Nucleoid-Associated Proteins On Dna Supercoiling In Escherichia Coli And Salmonella Typhimurium, Andrews Agbleke
The Impact Of Transcription And Nucleoid-Associated Proteins On Dna Supercoiling In Escherichia Coli And Salmonella Typhimurium, Andrews Agbleke
All ETDs from UAB
Bacterial chromosomes are maintained under constant negative supercoiled stress that influences almost all cellular processes including DNA replication, transcription, segregation, site-specific and homologous recombination, and other DNA transposition. The average supercoiling density of the chromosome is generated by gyrase, and supercoil equilibrium is maintained by two other topoisomerases topo I and topo IV. The supercoils generated by gyrase can diffuse over 10 kb domains. The other half of the chromosome's supercoil structure is connected to abundant nucleoid-associated proteins (NAPs). My goal was to characterize factors other than gyrase that participate in generating or regulating supercoil dynamics in Escherichia coli and …
Determinant Of G-Chain Mediated Fc Receptor Signaling, Spandan Shah
Determinant Of G-Chain Mediated Fc Receptor Signaling, Spandan Shah
All ETDs from UAB
Fc receptors provide an interface between humoral immunity and cellular immunity by binding to the Fc portion of immunoglobulin and mediating effector functions. Cells expressing Fc receptors play a major role in immune complex clearance via phagocytosis, leading to a context dependent pro- or anti-inflammatory environment. Signaling by multi-chain Fc receptor complexes, including FcgRI, FcgRIII, FceRI and FcaRI is mediated by the ITAM-containing common FceRIg (g-chain) subunit. However, despite the use of a common g-chain, different Fc receptor complexes elicit specific cellular programs, suggesting additional layers of regulation of tyrosine based signaling by g-chain. We hypothesized that the unique g-chain …
Epigenetic Markers In The Developing Postnatal Brain, Rebecca Kaye Simmons
Epigenetic Markers In The Developing Postnatal Brain, Rebecca Kaye Simmons
All ETDs from UAB
Epigenetic mechanisms provide a critical and plausible mechanism by which genes and the environment can interact and have been implicated in a number of diseases and disorders. In order to better understand how epigenetic mechanisms go awry in a diseased brain we must first understand how epigenetic mechanisms unfold during normal development. The present body of work begins to examine the role of DNA methylation in normal development and how it may contribute to a rodent model of emotion dysfunction. Using a variety of techniques, we evaluated the transcript, protein and functional output levels of DNA methyltransferase1, -3a, and -3b …
Relationships Between Latino Parental, Adolescent And Cultural Variables On Adolescents' Attitudes, Norms, Self-Efficacy, And Sexual Intentions, Vanessa Pirani Gaioso
Relationships Between Latino Parental, Adolescent And Cultural Variables On Adolescents' Attitudes, Norms, Self-Efficacy, And Sexual Intentions, Vanessa Pirani Gaioso
All ETDs from UAB
The quantity and quality of parent-adolescent sexual communication has been suggested as the best predictor on adolescent sexual behavior. The purpose of this study was to test a theoretical model based on the Parent-Based Expansion of the Theory of Planned Behavior (PETPB) examining relationships between selected parental, adolescent and cultural variables and Latino adolescents' intentions to engage in sexual behavior. This study used a cross-sectional correlational design based on a secondary data analysis of 130 Latino parent and adolescent dyads. Regression and path analysis procedures were used to test seven research hypotheses and the results demonstrated partial support for the …
The Role Of Pneumococcal Choline-Binding Protein A (Pcpa) In The Mechanism Of Virulence And Protective Immunity, Melissa Marie Walker
The Role Of Pneumococcal Choline-Binding Protein A (Pcpa) In The Mechanism Of Virulence And Protective Immunity, Melissa Marie Walker
All ETDs from UAB
pcpA is under control of the Mn 2-dependent repressor PsaR. In the high manganese environment of mucosal sites pcpA gene expression is repressed and at the low manganese environment of the lung and blood derepression of pcpA gene expressionis observed. Previous data has shown that immunization of mice with rPcpA elicits protection against pneumonia and sepsis but had no effect on nasal colonization. We and others have shown that pcpA expression significantly enhances adherence of pneumococci to lung epithelial cell lines and although it is a virulence factor for pneumonia in mice there is no biologically relevant role for PcpA …
Cyclic Gmp Phosphodiesterase As A Novel Target For Colorectal Cancer Chemoprevention, Nan Li
Cyclic Gmp Phosphodiesterase As A Novel Target For Colorectal Cancer Chemoprevention, Nan Li
All ETDs from UAB
Nonsteroidal anti-inflammatory drugs (NSAIDs) display promising antineoplastic activity for colorectal and other cancers, but toxicity from cyclooxygenase (COX) inhibition limits their long-term use for chemoprevention. However, many investigators have concluded that COX-independent mechanisms are responsible for their antineoplastic activity. We previously reported that cyclic guanosine monophosphate phosphodiesterase (cGMP PDE) is a non-COX target of sulindac sulfide (SS). However, the specific isozyme families involved have not been well studied. Here we demonstrate that SS inhibits the growth of colon tumor cells through a novel mechanism involving inhibition of two cGMP degrading isozymes, PDE5 and PDE10, to activate cGMP-dependent protein kinase (PKG). …
Spatial Analysis Of Cardiovascular Mri Data, Samantha Seals
Spatial Analysis Of Cardiovascular Mri Data, Samantha Seals
All ETDs from UAB
The goal of this dissertation is to investigate applying currently available Frequentist spatial analysis methods to cardiovascular MRI (cMRI) data. This data is typically analyzed using methods dependent summary statistics; our goal is to analyze using the full dataset. In our first paper, we explore application of spatial covariance structures to cMRI data via a generalized linear model. In our second paper, we examine the effects of misspecification of the covariance structure when analyzing spatially correlated data utilizing generalized linear models via Monte Carlo simulation. Finally, in our third paper, we propose an alternative family of covariance structures that lends …
Role Of Kca3.1, A Calcium-Activated Potassium Channel, In Cell Migration In The Adult Brain, Kathryn L. Turner
Role Of Kca3.1, A Calcium-Activated Potassium Channel, In Cell Migration In The Adult Brain, Kathryn L. Turner
All ETDs from UAB
A limited amount of migration occurs in the adult brain, including migration of neuroblasts during adult neurogenesis and pathological migration of primary brain tumors. Ion channels are commonly utilized during migration to allow shape and volume changes required for cell movement, and we specifically examine the role of KCa3.1 in the adult brain. These channels are well-suited to facilitate movement because they respond to common migration signals, i.e. intracellular Ca2+ changes, and efflux K+, allowing the outflow of water and cell volume regulation. Our findings suggest that KCa3.1 is highly expressed in migratory cells, adult neuroblasts and glioma cells, but …
Studies On Distracted Driving: Three Papers Examining The State Of The Literature And The Impact Of Texting Restrictions On Motor Vehicle Crash-Related Fatalities And Hospitalizations, Alva Oletia Ferdinand
Studies On Distracted Driving: Three Papers Examining The State Of The Literature And The Impact Of Texting Restrictions On Motor Vehicle Crash-Related Fatalities And Hospitalizations, Alva Oletia Ferdinand
All ETDs from UAB
ABSTRACT This three-paper dissertation addresses gaps in the literature focused on distracted driving and the impact of regulations aimed at reducing its prevalence. With the widespread proliferation of mobile phone usage and more sophisticated in-vehicle entertainment and information systems, it is now commonplace for drivers to engage in non-driving activities (also referred to as "secondary tasks") while driving. It is widely believed that distracted driving is positively related to motor vehicle crashes and other undesirable driving outcomes. This dissertation specifically focuses on three topics: (1) the nature of the distracted driving literature given the numerous secondary tasks, driving performance outcomes, …
Comparison Of Fabricated Carbon Nanotube Sorbent Felts And 3m Charcoal Sorbent Wafers To Assess Passive Sampling, Samantha Connell
Comparison Of Fabricated Carbon Nanotube Sorbent Felts And 3m Charcoal Sorbent Wafers To Assess Passive Sampling, Samantha Connell
All ETDs from UAB
Passive air sampling is the preferred method of air sampling for many applications. Traditionally, this method uses activated charcoal as the sorbent and samples are analyzed by chemical or thermal desorption. This research explores the use of carbon nanotubes in the form of a felt as a sorbent for passive sampling by comparing the mass uptake and percent yield to that of the 3MTM Organic Vapor Monitor (OVM) 3500 over four time trials. The desorption efficiency, adsorption capacity, and mass uptake of the fabricated felts are very similar to the 3MTM OVM 3500. It was expected that the fabricated felts …
The Role Of O-Glcnacylation In Synaptic Function, Erica Wynette Taylor
The Role Of O-Glcnacylation In Synaptic Function, Erica Wynette Taylor
All ETDs from UAB
O-GlcNAcylation is a dynamic protein posttranslational modification that adds the monosaccharide ß-N-acetylglucosamine (GlcNAc) to specific serine and threonine residues on nucleocytoplasmic proteins. The hippocampus is a part of brain that has been shown to play a crucial role in learning and memory. Hippocampal neurons have high expression of O-GlcNAc transferase and O-GlcNAcase, which catalyze the addition and removal of GlcNAc from proteins respectively. Moreover, synaptic plasticity which is the strengthening or weakening of synaptic function on short- and long-term time scales is believed to be a cellular correlate of learning and memory. Several forms of synaptic plasticity have been characterized …
Phenotypic Analysis Of B Cells In Hla*B44 Positive Patients That Exhibit Common Variable Immunodeficiency And Recurrent Sino-Pulmonary Infections, Eva Szymanska Mroczek
Phenotypic Analysis Of B Cells In Hla*B44 Positive Patients That Exhibit Common Variable Immunodeficiency And Recurrent Sino-Pulmonary Infections, Eva Szymanska Mroczek
All ETDs from UAB
Depressed serum immunoglobulin levels and recurrent sinopulmonary infections mark Common Variable Immune Deficiency (CVID). Many family members of CVID patients also suffer Recurrent Sino-Pulmonary Infection (RESPI) but have normal serum immunoglobulins. We identified HLAB44 positive identical female twins who suffer sinopulmonary infections and are discordant for CVID and RESPI. Flow cytometry subsets showed equivalent numbers of immature B cells (BC) in both twins, but lower numbers of transitional and mature BC in the CVID twin. Presented is an in-depth comparison of V (D) J gene usage, hydrophobicity, length, DH reading frame, and amino acid usage between µ H chain repertoires …
Insulin Resistance, Lipids, And The Metabolic Syndrome Trait Cluster: Impact Of Race And Very Low Calorie Diet, Veeradej Pisprasert
Insulin Resistance, Lipids, And The Metabolic Syndrome Trait Cluster: Impact Of Race And Very Low Calorie Diet, Veeradej Pisprasert
All ETDs from UAB
Insulin resistance (IR) is central to the pathogenesis of cardiometabolic disorders. Early detection, along with appropriate interventions, is paramount to reduce the morbidity and mortality of IR-related diseases. The gold standard measurement for IR is the hyperinsulinemic-euglycemic clamp, which is markedly sophisticated for clinical evaluation but costly and invasive for clinical practice. Several alternative surrogate indicators of IR have been identified and used by clinicians, including insulin sensitivity indices derived from plasma glucose and insulin values, as well as other indicators that are independent of direct insulin measurements. In addition, lipid panel and lipoprotein subclass profile have emerged as markers …
The Effects Of Markers For Hiv Disease Progression, Social Network, Barriers To Care, Depression, And Substance Abuse On Medical Appointment Adherence Of Hiv-Infected Adults, Yeow Chye Ng
All ETDs from UAB
The study was designed to test a causal model of theoretical relationships among markers for HIV disease progression, social network, barriers to care, depression, substance abuse, and medical appointment adherence among HIV-infected adults. The proposed causal model was derived and developed from Lazarus and Folkman's theory of stress, appraisal, and coping. The knowledge gained from this study may yield critical information with respect to determining how a health care provider or researcher can develop specific behavioral interventions for the improvement of medical appointment adherence among HIV-infected adults living in the Southeastern United States. The correlational study was a secondary analysis …
Effect Of A Faith-Based Initiative On Hospital Readmissions, Joseph Webb
Effect Of A Faith-Based Initiative On Hospital Readmissions, Joseph Webb
All ETDs from UAB
EFFECT OF A FAITH-BASED INITIATIVE ON HOSPITAL READMISSIONS JOSEPH WEBB ADMINISTRATION-HEALTH SERVICES ABSTRACT The purpose of this study was to examine the relationship between a faith-based initiative and hospital readmissions. The main data source used was the inpatient data-base of Methodist LeBonheur Healthcare System (MLH) in Memphis, TN. Data were collected from admissions that occurred during years 2008 through 2012 at four of MLH's five adult acute-care hospitals. Data from the U.S. Census Bureau's American Community Survey were also used in the study. This study uses theoretical tenets from the Social Justice and Equity Theory and the Chronic Care Model …