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Dna Barcoding Of Freshwater Fishes And The Development Of A Quantitative Qpcr Assay For The Species-Specific Detection And Quantification Of Fish Larvae From Plankton Samples, William Loh, P Bond, Kevin Ashton, David Roberts, I.R. Tibbetts
Dna Barcoding Of Freshwater Fishes And The Development Of A Quantitative Qpcr Assay For The Species-Specific Detection And Quantification Of Fish Larvae From Plankton Samples, William Loh, P Bond, Kevin Ashton, David Roberts, I.R. Tibbetts
Kevin Ashton
The barcoding of mitochondrial cytochrome c oxidase subunit 1 (coI) gene was amplified and sequenced from 16 species of freshwater fishes found in Lake Wivenhoe (south-eastern Queensland, Australia) to support monitoring of reservoir fish populations, ecosystem function and water health. In this study, 630-650 bp sequences of the coI barcoding gene from 100 specimens representing 15 genera, 13 families and two subclasses of fishes allowed 14 of the 16 species to be identified and differentiated. The mean ± s.e. Kimura 2 parameter divergence within and between species was 0·52 ± 0·10 and 23·8 ± 2·20% respectively, indicating that barcodes can …