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High-Throughput Sequencing Of Plasma Microrna In Chronic Fatigue Syndrome/Myalgic Encephalomyelitis, Ekua Brenu, Kevin Ashton, Jana Batovska, Don Staines, Sonja Marshall-Gradisnik
High-Throughput Sequencing Of Plasma Microrna In Chronic Fatigue Syndrome/Myalgic Encephalomyelitis, Ekua Brenu, Kevin Ashton, Jana Batovska, Don Staines, Sonja Marshall-Gradisnik
Kevin Ashton
Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME. Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation …