Medicine and Health Sciences Commons^™

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- ADRDL Steps Up in Light of Highly Pathogenic H5N2 Avian Influenza Outbreaks [Page] 2- Director's Message: The SD Animal Disease Research and Diagnostic Laboratory and Emergency Testing for Highly Pathogenic Avian Influenza [Page] 3- Pre-Veterinary Students Honored by SDVMA and SDSU Through Stethoscope Ceremony [Page] 4- Clerical Staff Makes Strides in Efficiency and Service [Page] 5- ADRDL Pathologists Team with Veterinarian to Address Cattle Lameness for Livestock Group [Page] 5- Canine Influenza Testing—SDSU ADRDL [Page] 6- ADRDL Hosts Tours for Legislators and State Agency Staff [Page] 6- ADRDL Offers Next Generation Sequencing [Page] 6- ADRDL at the SDVMA …

Fecal Volatile Organic Ccompound Profiles From White-Tailed Deer (Odocoileus Virginianus) As Indicators Of Mycobacterium Bovis Exposure Or Mycobacterium Bovis Bacille Calmette-Guerin (Bcg) Vaccination, Randal S. Stahl, Christine K. Ellis, Pauline Nol, W. Ray Waters, Kurt C. Vercauteren

USDA Wildlife Services: Staff Publications

White-tailed deer (Odocoileus virginianus) serve as a reservoir for bovine tuberculosis, caused by Mycobacterium bovis, and can be a source of infection in cattle. Vaccination with M. bovis Bacille Calmette Guerin (BCG) is being considered for management of bovine tuberculosis in deer. Presently, no method exists to non-invasively monitor the presence of bovine tuberculosis in deer. In this study, volatile organic compound profiles of BCG-vaccinated and non-vaccinated deer, before and after experimental challenge with M. bovis strain 95–1315, were generated using solid phase microextraction fiber head-space sampling over suspended fecal pellets with analysis by gas chromatography/mass spectrometry. …

The First Shared Online Curriculum Resources For Veterinary Undergraduate Learning And Teaching In Animal Welfare And Ethics In Australia And New Zealand, Jane Johnson, Teresa Collins, Chris Degeling, Anne Fawcett, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jennifer Hood, Jennifer K.F. Lloyd, Clive J.C. Phillips, Kevin J. Stafford

Professional Veterinary Ethics Collection

The need for undergraduate teaching of Animal Welfare and Ethics (AWE) in Australian and New Zealand veterinary courses reflects increasing community concerns and expectations about AWE; global pressures regarding food security and sustainability; the demands of veterinary accreditation; and fears that, unless students encounter AWE as part of their formal education, as veterinarians they will be relatively unaware of the discipline of animal welfare science. To address this need we are developing online resources to ensure Australian and New Zealand veterinary graduates have the knowledge, and the research, communication and critical reasoning skills, to fulfill the AWE role demanded of …

La Souffrance Animale À Distance: Des Vétérinaires Dans L’Action Humanitaire, Frédéric Keck, Miriam Ticktin

Publications and Research

Résumé

Cet article étudie le rôle des vétérinaires dans l’humanitaire à partir de deux types de pratique : la défense des animaux contre la cruauté et la surveillance des animaux touchés par les épidémies. En suivant l’extension de l’action humanitaire aux animaux comme nouvelles figures de victimes innocentes, nous cherchons à dépasser l’approche compassionnelle de l’humanitaire pour étudier les nouvelles formes scientifiques impliquant des non-humains, comme la médecine vétérinaire légale, les neurosciences et l’immunologie. Nous soutenons finalement que ces sciences produisent de nouveaux collectifs d’humains et de non-humains.

Abstract

This article traces the role of veterinarians in humanitarian action, focusing …

Complete Genome Sequence Of A Highly Pathogenic Avian Influenza Virus (H5n2) Associated With An Outbreak In Commercial Chickens, Iowa, Usa, 2015, Travis Clement, Gerald F. Kutish, Jill Nezworski, Joy Scaria, Eric A. Nelson, Jane Christopher-Hennings, Diego Diel

Veterinary and Biomedical Sciences Faculty Publications

A novel reassortant influenza A virus (H5N2) was first detected in British Columbia, Canada, in December 2014. The virus rapidly spread along the waterfowl migration flyways in the United States, causing multiple HPAI outbreaks in poultry. Here, we present the complete genome sequence of HPAIV-H5N2 from a commercial chicken flock in Iowa.

Development Of An In Vivo Exsheathment Assay Of Infective L3 Haemonchus Contortus Larvae In Fistualated Sheep, Holly N. Williams, Katherine Petersson, Carly Barone

Senior Honors Projects

Purpose: Gastrointestinal nematodes (GIN) hinder the sustainable production of small ruminants on pasture and parasite resistance to chemical dewormers is becoming a growing concern. Condensed tannin containing legume forages are being tested to evaluate their anti-parasitic properties and potential contribution to an overall parasite control program for small ruminants such as sheep and goats. One of the most pathogenic GIN of small ruminants is Haemonchus contortus. The final step to full infectivity of H. contortus third stage larvae (L3) is exsheathment in the rumen. The objective of this study was to establish an in-vivo exsheathment assay in fistulated sheep …

Osteomyelitis Associated With Nocardiopsis Composta In A Dog, Elisa N. Salas, Debra Royal, Lance Kurz, J. Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Osteomyelitis can be caused by bacterial or fungal agents or may be idiopathic. Cocci, bacilli, and filamentous bacteria such as members of the Actinomycetes have all been determined to be causes of osteomyelitis. Differential diagnoses for Gram-positive filamentous rods in the family Actinomycetales causing osteomyelitis in the dog, include members of the more frequently encountered genera Nocardia and Actinomyces. Bacteria gain access to the bone via several routes but are most often associated with direct inoculation (such as percutaneous injuries, compound fractures, or secondary to foreign bodies such as surgical or other material including dirt and wood) and fracture …

Microhabitat Use Affects Brain Size And Structure In Intertidal Gobies, Gemma E. White, Culum Brown

Veterinary Science and Medicine Collection

The ecological cognition hypothesis poses that the brains and behaviours of individuals are largely shaped by the environments in which they live and the associated challenges they must overcome during their lives. Here we examine the effect of environmental complexity on relative brain size in 4 species of intertidal gobies from differing habitats. Two species were rock pool specialists that lived on spatially complex rocky shores, while the remainder lived on dynamic, but structurally simple, sandy shores. We found that rock pool-dwelling species had relatively larger brains and telencephalons in particular, while sand-dwelling species had a larger optic tectum and …

Prevalence Of Disorders Recorded In Cavalier King Charles Spaniels Attending Primary-Care Veterinary Practices In England, Jennifer F. Summers, Dan G. O'Neill, David B. Church, Peter C. Thomson, Paul D. Mcgreevy, David C. Brodbelt

Epidemiology Collection

Background: Concerns have been raised over breed-related health issues in purebred dogs, but reliable prevalence estimates for disorders within specific breeds are sparse. Electronically stored patient health records from primary-care practice are emerging as a useful source of epidemiological data in companion animals. This study used large volumes of health data from UK primary-care practices participating in the VetCompass animal health surveillance project to evaluate in detail the disorders diagnosed in a random selection of over 50% of dogs recorded as Cavalier King Charles Spaniels (CKCSs). Confirmation of breed using available microchip and Kennel Club (KC) registration data was attempted. …

Prevalence Of And Risk Factors For Degenerative Mitral Valve Disease In Dogs Attending Primary‐Care Veterinary Practices In England, M. Mattin, A. Boswood, D. B. Church, J. López-Alvarez, P. D. Mcgreevy, D. G. O'Neill, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Background

To date, epidemiological studies on degenerative mitral valve disease (DMVD) in dogs have largely reported referral caseloads or been limited to predisposed breeds. Analysis of primary‐care data to identify factors associated with DMVD would help clinicians identify high‐risk individuals and improve understanding.

Objectives

To estimate the prevalence of and identify risk factors for DMVD in dogs attending primary‐care veterinary practices in England.

Animals

Cases were identified within the electronic patient records of 111,967 dogs attending 93 practices. Four hundred and 5 dogs were diagnosed with DMVD (diagnosed cases) and a further 3,557 dogs had a heart murmur (HM) consistent …

A Behavioral Analysis Of The Pre-Release Tasmanian Devils (Sarcophilus Harrisii) Currently At Devils@Cradle: An Assessment Of The Impacts Of Captivity On The Wild Behaviors Of Tasmanian Devils That Are Important For Their Reintroduction, Survival, And Inclusion In Wild Populations, Anna Staudenmaier

Independent Study Project (ISP) Collection

The Tasmanian devil, Sarcophilus harrisi, is in danger of extinction in the wild due to the emergence of Devil Facial Tumor Disease (DFTD). In an attempt to save the species the Save the Tasmanian Devil Program (STTDP) has initiated the creation of an ‘Insurance Population’. These insurance animals are a part of the captive breeding population (CBP) designed to conserve the genetic diversity of the species to preserve their ecological function for their future reintroduction into the wild. CBPs are located at various bio-secure zoos, wildlife parks, free-range enclosures (FREs), Tasmanian islands and peninsulas and sanctuaries like Devils@Cradle - Tasmanian …

Detection Of Streptococus Equi From Environmental Samples Using An Elisa, Taryn Haller

Honors College

Equine Strangles, an upper respiratory disease caused by Streptococcus equi subspecies equi (S. equi) causes significant losses in the horse industry. Surveillance for S. equi could be facilitated by quantifying S. equi in environmental samples. The objective of this project was to evaluate ELISA in quantifying S. equi using two monoclonal antibodies (MAB’s) to the SeM protein (MAB-212 for capture, and biotinylated MAB-211 for detection), with Streptococcal phage lysin releasing SeM from the cell surface. Initial results confirmed a fresh culture of S. equi and two S. equi lysates stored at -20 C for 2 years as positive, while confirming …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- Latest Version of South Dakota “One Health” Meetings Explores Tuberculosis in Humans and Cattle [Page] 2- Director's Message [Page] 2- Bailey Herd Health Conference Covers Connection Between Facilities and Animal Health [Page] 3- Essential Behind the Scenes Work: Accounting and Human Resources Staff at the VBS and ADRDL [Page] 3- New Hires at South Dakota ADRDL [Page] 4- Dairy Animal and Product Testing Services at the ADRDL [Page] 6- Video Production Highlights ADRDL Value to Animals and Citizens [Page] 6- Bovine Respiratory Coronavirus Testing [Page] 6- Dr. Hennings Named to AAVLD Executive Board [Page] 6- Equine Well-Being Survey: …

An Inactivated Vaccine Made From A U.S. Field Isolate Of Porcine Epidemic Disease Virus Is Immunogenic In Pigs As Demonstrated By A Dose-Titration, Emily A. Collin, Srivishnupriya Anbalagan, Faten Okda, Ron Bateman, Eric A. Nelson, Ben M. Hause

Veterinary and Biomedical Sciences Faculty Publications

Background: Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the objective of this study was to evaluate the humoral immune response to the selected inactivated PEDV vaccine candidate in a dose-titration manner.
Results: PEDV was isolated from a pig with diarrhea and complete genome sequencing found >99% …

Relationship Between Heat-Labile Enterotoxin Secretion Capacity And Virulence In Wild Type Porcine-Origin Enterotoxigenic Escherichia Coli Strains, Prageeth Wijemanne, Jun Xing, Emil M. Berberov, David B. Marx, David H. Francis, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system(T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme- linked immunosorbent assay tomeasure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2), had …

Environmental Microbes And Uveitis: Is Microbial Exposure Always Bad?, Chandirasegaran Massilamany, A. Gangaplara, J. Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

The eye generally is considered to be an immune-privileged organ, but this notion is being increasingly challenged as ocular antigens can be expressed in the generative lymphoid organs, resulting in attainment of self-tolerance. What triggers a break in this tolerant state is a fundamental question in autoimmunity research. The general belief is that exposure to environmental microbes can break self-tolerance in genetically susceptible individuals, leading to the induction of autoimmune responses. The molecular mimicry hypothesis has been proposed as one major mechanistic, pathway through which microbes, by generating crossreactive immune responses, can induce ocular damage of the kind that might …

Diagnosis Of Strongylus Vulgaris, Martin K. Nielsen, Ulla Anderson, Daniel K. Howe

Veterinary Science Faculty Patents

Embodiments of the presently-disclosed subject matter provide an isolated polypeptide comprising the sequence of SEQ ID NO: 1, fragments thereof, and/or epitopes thereof. Embodiments of the presently-disclosed subject matter also provide methods for diagnosing a Strongylus vulgaris infection in a subject that comprises providing a biological sample from the subject and contacting the sample with the present isolated polypeptide, fragment thereof, and/or epitope thereof. In some embodiments theStrongylus vulgaris infection can be detected during a prepatent period. In further embodiments the subject is a horse.

Systems-Based Analysis Of The Sarcocystis Neurona Genome Identifies Pathways That Contribute To A Heteroxenous Life Cycle, Tomasz Blazejewski, Nirvana Nursimulu, Viviana Pszenny, Sriveny Dangoudoubiyam, Sivaranjani Namasivayam, Melissa A. Chiasson, Kyle Chessman, Michelle Tonkin, Lakshmipuram S. Swapna, Stacy S. Hung, Joshua Bridgers, Stacy M. Ricklefs, Martin J. Boulanger, Jitender P. Dubey, Stephen F. Porcella, Jessica C. Kissinger, Daniel K. Howe, Michael E. Grigg, John Parkinson

Veterinary Science Faculty Publications

Sarcocystis neurona is a member of the coccidia, a clade of single-celled parasites of medical and veterinary importance including Eimeria, Sarcocystis, Neospora, and Toxoplasma. Unlike Eimeria, a single-host enteric pathogen, Sarcocystis, Neospora, and Toxoplasma are two-host parasites that infect and produce infectious tissue cysts in a wide range of intermediate hosts. As a genus, Sarcocystis is one of the most successful protozoan parasites; all vertebrates, including birds, reptiles, fish, and mammals are hosts to at least one Sarcocystis species. Here we sequenced Sarcocystis neurona, the causal agent of fatal equine protozoal myeloencephalitis. The …

Ectoparasitism Shortens The Breeding Season In A Colonial Bird, Charles R. Brown, Mary Bomberger Brown

School of Natural Resources: Faculty Publications

When blood-feeding parasites increase seasonally, their deleterious effects may prevent some host species, especially those living in large groups where parasites are numerous, from reproducing later in the summer. Yet the role of parasites in regulating the length of a host’s breeding season—and thus the host’s opportunity for multiple brooding—has not been systematically investigated. The highly colonial cliff swallow (Petrochelidon pyrrhonota), a temperate-latitude migratory songbird in the western Great Plains, USA, typically has a relatively short (eight to nine week) breeding season, with birds rarely nesting late in the summer. Colonies at which ectoparasitic swallow bugs (Oeciacus vicarius) were experimentally …

Prevalence And Risk Factors For Mast Cell Tumours In Dogs In England, Stephanie J.W. Shoop, Stephanie Marlow, David B. Church, Kate English, Paul D. Mcgreevy, Anneliese J. Stell, Peter C. Thomson, Dan G. O'Neill, David C. Brodbelt

Epidemiology Collection

Background

Mast cell tumour (MCT) appears to be a frequent tumour type in dogs, though there is little published in relation to its frequency in dogs in the UK. The current study aimed to investigate prevalence and risk factors for MCTs in dogs attending English primary-care veterinary practices.

Methods

Electronic patient records from practices participating in the VetCompass animal surveillance project between July 2007 and June 2013 were searched for MCT diagnosis. Various search terms and standard diagnostic terms (VeNom codes) identified records containing MCT diagnoses, which were evaluated against clinical criteria for inclusion to the study. MCT prevalence for …

Protective Efficacy Of Centralized And Polyvalent Envelope Immunogens In An Attenuated Equine Lentivirus Vaccine, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David Horohov, Charles J. Issel, Ronald C. Montelaro

Maxwell H. Gluck Equine Research Center Faculty Publications

Lentiviral Envelope (Env) antigenic variation and related immune evasion present major hurdles to effective vaccine development. Centralized Env immunogens that minimize the genetic distance between vaccine proteins and circulating viral isolates are an area of increasing study in HIV vaccinology. To date, the efficacy of centralized immunogens has not been evaluated in the context of an animal model that could provide both immunogenicity and protective efficacy data. We previously reported on a live-attenuated (attenuated) equine infectious anemia (EIAV) virus vaccine, which provides 100% protection from disease after virulent, homologous, virus challenge. Further, protective efficacy demonstrated a significant, inverse, linear relationship …

Mutations In The 5’ Ntr And The Non-Structural Protein 3a Of The Coxsackievirus B3 Selectively Attenuate Myocarditogenicity, Chandirasegaran Massilamany, Arunakumar Gangaplara, Rakesh H. Basavalingappa, Rajkumar A. Rajasekaran, Hiep Vu, Jean-Jack Riethoven, David Steffen, Asit K. Pattnaik, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

The 5’ non-translated region (NTR) is an important molecular determinant that controls replication and virulence of coxsackievirus B (CVB)3. Previous studies have reported many nucleotide (nt) sequence differences in the Nancy strain of the virus, including changes in the 5’ NTR with varying degrees of disease severity. In our studies of CVB3-induced myocarditis, we sought to generate an infectious clone of the virus for routine in vivo experimentation. By determining the viral nt sequence, we identified three new nt substitutions in the clone that differed from the parental virus strain: C97U in the 5’ NTR; a silent mutation, A4327G, in …

Association Of Autophagy In The Cell Death Mediated By Dihydrotestosterone In Autoreactive T Cells Independent Of Antigenic Stimulation, Ting Jia, Annandurai Anandhan, Chandirasegaran Massilamany, Rajkumar A. Rajasekaran, Rodrigo Franco, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Gender disparity is well documented in the mouse model of experimental autoimmune encephalomyelitis (EAE) induced with proteolipid protein (PLP) 139–151, in which female, but not male, SJL mice show a chronic relapsing-remitting paralysis. Furthermore, dihydrotestosterone (DHT) has been shown to ameliorate the severity of EAE, but the underlying mechanisms of its protective effects are unclear. Using major histocompatibility complex (MHC) class II dextramers for PLP 139-151, we tested the hypothesis that DHT selectively modulates the expansion and functionalities of antigen-specific T cells. Unexpectedly, we noted that DHT induced cell death in antigen-specific, autoreactive T cells, but the effects were not …

Evaluation Of An Interactive Workshop Designed To Teach Practical Welfare Techniques To Beef Cattle Caretakers And Decision Makers, Reneé Dewell, Christy Hanthorn, Jared Danielson, Rebecca Burzette, Johann Coetzee, D. Dee Griffin, Alejandro Ramirez, Grant Dewell

School of Veterinary and Biomedical Sciences: Faculty Publications

The purpose of the project was to evaluate the use of an interactive workshop designed to teach novel practical welfare techniques to beef cattle caretakers and decision makers. Following training, respondents reported being more likely to use or recommend use of local anesthesia for dehorning and castration and were more inclined to use meloxicam for pain management. Respondents also reported improvement in performing all skills taught in the workshop. These results suggest instructional techniques used to teach practical concepts/techniques relevant to beef cattle welfare led to improvements in both perceived competency and estimated likelihood that the material will be used. …

Exogenous Administration Of Lipids To Steers Alters Aspects Of The Innate Immune Response To Endotoxin Challenge, Nicole C. Burdick Sanchez, Jeffery A. Carroll, Janet R. Donaldson, Joe O. Buntyn, Ty B. Schmidt

Department of Animal Science: Faculty Publications

This study examined the effects of increasing energy availability from both dextrose and lipid treatments on the proinflammatory response to LPS in Holstein steers. Steers were randomly assigned to one of three groups: saline at 0.5 ml/ kg body weight (Control) or 50% dextrose [0.5 ml/kg body weight (Dextrose) to mimic calm cattle’s response to LPS] administered immediately prior to LPS (0.5 mg/kg body weight at 0 h) or continuous lipid emulsion infusion from –1 to 6 h [Intralipid 20% (Baxter, Deerfield, IL USA); 0.5 ml/kg/hr (Lipid) to mimic temperamental cattle]. Concentrations of non-esterified fatty acids (NEFA) were greater in …

Histological Sequences Of Long Bone Development In The New Zealand White Rabbits, Yasser A. Ahmed, Eman A. Abdelrahim, Fatma Khalil

Histology Collection

Long bone development occurred through endochondral ossification. The current study investigated the main histological changes associated with the development of humerus from the New Zealand white rabbits. The upper limb or humerus during embryonic (12, 14, 15, 16, 18, 21, 24 and 27 days) and neonatal (3 and 7 days) development was processed for light microscopy. Three stages preceded endochondral ossification; limb bud (mesenchymal cells), cartilage primordia (mesenchymal condensation and differentiation into chondrocytes) and cartilage template (organization of chondrocytes into proliferative and hypertrophic zones) by 12, 14 and 15 days, respectively. At day 16, cartilage template elongated forming a cartilaginous …

Relationship Between Heat-Labile Enterotoxin Secretion Capacity And Virulence In Wild Type Porcine-Origin Enterotoxigenic Escherichia Coli Strains, Prageeth Wijemanne, Jun Xing, Emil M. Berberov, David H. Francis, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Heat-labile enterotoxin (LT) is an important virulence factor secreted by some strains of enterotoxigenic Escherichia coli (ETEC). The prototypic human-origin strain H10407 secretes LT via a type II secretion system(T2SS). We sought to determine the relationship between the capacity to secrete LT and virulence in porcine-origin wild type (WT) ETEC strains. Sixteen WT ETEC strains isolated from cases of severe diarrheal disease were analyzed by GM1ganglioside enzyme- linked immunosorbent assay tomeasure LT concentrations in culture supernatants. All strains had detectable LT in supernatants by 2 h of culture and 1 strain, which was particularly virulent in gnotobiotic piglets (3030-2), had …

Pseudorabies Virus Fast Axonal Transport Occurs By A Pus9- Independent Mechanism, Gina R. Daniel, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Reactivation from latency results in transmission of neurotropic herpesviruses from the nervous system to body surfaces, referred to as anterograde axonal trafficking. The virus-encoded protein pUS9 promotes axonal dissemination by sorting virus particles into axons, but whether it is also an effector of fast axonal transport within axons is unknown. To determine the role of pUS9 in anterograde trafficking, we analyzed the axonal transport of pseudorabies virus in the presence and absence of pUS9.

Haemorrhagic Colitis Associated With Enterohaemorrhagic Escherichia Coli O165:H25 Infection In A Yearling Feedlot Heifer, Rodney A. Moxley, Zachary R. Stromberg, Gentry L. Lewis, John D. Loy, Bruce W. Brodersen, Isha R. Patel, Jayanthi Gangiredla

School of Veterinary and Biomedical Sciences: Faculty Publications

Introduction: Enterohaemorrhagic Escherichia coli (EHEC) cause haemorrhagic colitis and haemolytic uraemic syndrome in humans. Although EHEC infection typically results in haemorrhagic colitis in all ages of human patients, in cattle it is usually limited to 1- to 5-week-old nursing calves.

Case Presentation: A 1-year-old feedlot beef heifer was moribund with neurological signs and bloody diarrhoea. At necropsy, the colonic mucosa contained multiple grossly visible haemorrhagic erosions, each measuring <1 mm in diameter. Histologically, foci corresponding to the gross erosions had E. coli O165 antigen-positive bacterial rods adherent to the apical surfaces of degenerate and necrotic colonic mucosal epithelial cells in association with attaching and effacing lesions, and also within cytoplasmic vacuoles in …

Temperatures Of Storage Areas In Large Animal Veterinary Practice Vehicles In The Summer And Comparison With Drug Manufacturers’ Storage Recommendations, Jeff D. Ondrak, Meredyth L. Jones, Virginia R. Fajt

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Large animal veterinarians carry drugs in their practice vehicles in storage areas that are not typically refrigerated. The most common upper limits of manufacturers’ storage temperatures for United States (U.S.)-approved non-refrigerated drugs are 25 or 30 °C. Because ambient temperatures in many locations in the U.S. exceed these temperatures during the summer, we measured storage area temperatures over 4 months in the summer of 2013 to evaluate the extent to which labeled storage temperatures are exceeded.

Methods: A convenience sample of 12 vehicles from 5 central Texas practices and 12 vehicles from 4 south central Nebraska practices was …