Medicine and Health Sciences Commons^™

Reduced Intestinal Colonization Of Adult Beef Cattle By Escherichia Coli O157:H7 Tir Deletion And Nalidixic-Acid-Resistant Mutants Lacking Flagellar Expression, Gustavo Bretschneider, Emil M. Berberov, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The importance of the Escherichia coli O157:H7 translocated intimin receptor (Tir) protein in intestinal colonization and the effect of infection with Tir⁺ strains on protection against subsequent challenge was studied in adult beef cattle. Cattle were orally inoculated (C1) with a Shiga toxin-2⁺+ E. coli O157:H7 strain that was Tir⁺ or Tir^-, and 42 days later were re-challenged (C2) with the nalidixic acid (Nal) ^R parent strain to test whether prior infection had any effect on fecal shedding. During the first 14 days post-C1, the Nal^S wildtype (WT) strain was shed at significantly …

Porcine Intestinal Epithelial Cell Lines As A New In Vitro Model For Studying Adherence And Pathogenesis Of Enterotoxigenic Escherichia Coli, Seung Y. Koh, Sajan George, Volker Brozel, Rodney A. Moxley, David H. Francis, Radhey Kaushik

School of Veterinary and Biomedical Sciences: Faculty Publications

Enterotoxigenic Escherichia coli (ETEC) infections result in large economic losses in the swine industry worldwide. The organism causes diarrhea by adhering to and colonizing enterocytes in the small intestines. While much progress has been made in understanding the pathogenesis of ETEC, no homologous intestinal epithelial cultures suitable for studying porcine ETEC pathogenesis have been described prior to this report. In the current study, we investigated the adherence of various porcine ETEC strains to two porcine (IPEC-1 and IPEC-J2) and one human (INT-407) small intestinal epithelial cell lines. Each cell line was assessed for its ability to support the adherence of …

Stable Cell Lines Expressing High Levels Of The Herpes Simplex Virus Type 1 Lat Are Refractory To Caspase 3 Activation And Dna Laddering Following Cold Shock Induced Apoptosis, Dale Carpenter, Chinhui Hsiang, Donald J. Brown, Ling Jin, Nelson Osorio, Lbachir Benmohamed, Clinton J. Jones, Steven L. Wechsler

School of Veterinary and Biomedical Sciences: Faculty Publications

The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene’s anti-apoptosis activity plays a central, but not fully elucidated, role in enhancing the virus’s reactivation phenotype. In transient transfection experiments, LAT increases cell survival following an apoptotic insult in the absence of other HSV-1 genes. However, the high background of untransfected cells has made it difficult to demonstrate that LAT inhibits specific apoptotic factors such as caspases. Here we report that, in mouse neuroblastoma cell lines (C1300) stably expressing high levels of LAT, cold shock induced apoptosis was blocked as judged by increased survival, protection against DNA fragmentation …

Light-Evoked Calcium Responses Of Isolated Melanopsin- Expressing Retinal Ganglion Cells, Andrew T. E. Hartwick, Jayne R. Bramley, Jianing Yu, Kelly T. Stevens, Charles N. Allen, William H. Baldridge, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

A small number (<2%) of mammalian retinal ganglion cells express the photopigment melanopsin and are intrinsically photosensitive (ipRGCs). Light depolarizes ipRGCs and increases intracellular calcium levels ( [Ca²⁺]_i ) but the signaling cascades underlying these responses have yet to be elucidated. To facilitate physiological studies on these rare photoreceptors, highly enriched ipRGC cultures from neonatal rats were generated using anti-melanopsin-mediated plate adhesion (immunopanning). This novel approach enabled experiments on isolated ipRGCs, eliminating the potential confounding influence of rod/cone-driven input. Light induced a rise in [Ca²⁺]_i (monitored using fura-2 imaging) in the immunopanned ipRGCs and the source of this Ca²⁺ signal was investigated. The Ca²⁺ responses were inhibited by 2-aminoethoxydiphenyl borate, SKF-96365 (1–2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl-1H-imidazole), flufenamic acid, …

Neuroanatomy Of The Subadult And Fetal Brain Of The Atlantic White-Sided Dolphin (Lagenorhynchus Acutus) From In Situ Magnetic Resonance Images, Eric W. Montie, Gerald E. Schneider, Darlene R. Ketten, Lori Marino, Katie E. Touhey, Mark E. Hahn

Veterinary Science and Medicine Collection

This article provides the first anatomically labeled, magnetic resonance imaging (MRI) -based atlas of the subadult and fetal Atlantic white-sided dolphin (Lagenorhynchus acutus) brain. It differs from previous MRI-based atlases of cetaceans in that it was created from images of fresh, postmortem brains in situ rather than extracted, formalin-fixed brains. The in situ images displayed the classic hallmarks of odontocete brains: fore-shortened orbital lobes and pronounced temporal width. Olfactory structures were absent and auditory regions (e.g., temporal lobes and inferior colliculi) were enlarged. In the subadult and fetal postmortem MRI scans, the hippocampus was identifiable, despite the relatively small size …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- New Diagnostic/Research Faculty Bring Talents to SDSU, Dr. Diego Del, Dr. Joy Scaria [Page] 1- More New Hires to Start Soon at VBS Department [Page] 2- Director's Message: “One Health” and the Importance of Training the Next Generation of Scientists [Page] 3- Summary of BVDV-PI Testing at SDSU ADRDL [Page] 4- The Clinical Pathology Section at SDSU’s ADRDL [Page] 5- Governor Daugaard Pays Visit to the ADRDL Through Stethoscope Ceremony [Page] 6- New Hire: Dana Rausch, Molecular Diagnostics [Page] 6- ADRDL Bacteriologist Serves as President [Page] 6- Serology Staff Member Aids Custer State Park Bison Testing Efforts [Page] …

Allelic Heterogeneity At The Equine Kit Locus In Dominant White (W) Horses, Bianca Haase, Samantha A. Brooks, Angela Schlumbaum, Pedro J. Azor, Ernest Bailey, Ferial Alaeddine, Meike Mevissen, Dominik Burger, Pierre-André Poncet, Stefan Rieder, Tosso Leeb

Veterinary Science Faculty Publications

White coat color has been a highly valued trait in horses for at least 2,000 years. Dominant white (W) is one of several known depigmentation phenotypes in horses. It shows considerable phenotypic variation, ranging from approximately 50% depigmented areas up to a completely white coat. In the horse, the four depigmentation phenotypes roan, sabino, tobiano, and dominant white were independently mapped to a chromosomal region on ECA 3 harboring the KIT gene. KIT plays an important role in melanoblast survival during embryonic development. We determined the sequence and genomic organization of the approximately 82 kb equine KIT gene. A mutation …

2006 Michigan Dairy Farm Business Analysis Summary, Eric Wittenberg, Christopher Wolf

Michigan Bovine Tuberculosis Bibliography and Database

This report summarizes the financial and production records of 139 dairy farms from throughout Michigan in 2006. To be included, the farms must have produced at least 50 percent of gross cash farm income from milk and dairy animal sales. The records came from Michigan State University’s TelFarm project and the Farm Credit Service system in Michigan. The values were pooled into averages for reporting purposes. The farms are larger than would be the average of all dairy farms in Michigan. While considerable variation in the data exists, average values are reported in the summary tables and discussion that follows.

Differences In Virulence Among Escherichia Coli O157:H7 Strains Isolated From Humans During Disease Outbreaks And From Healthy Cattle, Diane R. Baker, Rodney A. Moxley, Mike B. Steele, Jeffrey T. Lejeune, Jane Christopher-Hennings, Ding-Geng Chen, Philip R. Hardwidge, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Escherichia coli O157:H7 causes life-threatening outbreaks of diarrhea, hemorrhagic colitis, and hemolyticuremic syndrome in humans and significant economic loss in agriculture and could be a potential agent of bioterrorism. Although the prevalence of E. coli O157:H7 in cattle and other species with which humans have frequent contact is high, human infections are relatively uncommon, despite a low infectious dose. A plausible explanation for the low disease incidence is the possibility that not all strains are virulent in humans. If there are substantial differences in virulence among strains in nature, then human disease may select for high virulence. We used a …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1- The Survey Says Diagnostic News [Page] 1- Tritrichomonas Testing and PCR [Page] 2- Update: Viral Hemorrhagic Septicemia [Page] 2- Addition to Compounding Pharmacy Listing [Page] 2- Holiday Hours Extension News [Page] 2- Bull Tail-Tip Necrosis Cases in South Dakota, Summer, 2007 [Page] 5- Microchips and Cancer – AVMA Statement [Page] 5- Studies: New Treatments for Horses with Navicular Disease [Page] 5- Pieces and Parts Research News [Page] 6- Research Spotlight: Dr. Weiping Zhang Student News [Page] 7- Incoming freshmen pre-vets at SDSU [Page] 8- Calendar of Events

Enhancement Of Efficacy Of Prrsv Vaccines By Altering The Glycosylation Pattern Of Viral Glycoproteins, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

PRRSV, the causative agent of PRRS is of major economic significance to the pork industry in the USA and around the world. Current commercial vaccine does not provide adequate protection against PRRSV outbreaks. Therefore, there is an urgent need for development of more efficacious vaccine to combat PRRS. Our previous studies have suggested that (i) induction of neutralizing antibody response is an important correlate of evaluating the efficacy of a vaccine; (ii) neutralizing antibodies can be enhanced by hypoglycosylation of the major surface glycoprotein (GP5). While it is known that GP5 plays a prominent role in neutralizing antibody induction, it …

Febrile Response And Decrease In Circulating Lymphocytes Following Acute Infection Of White-Tailed Deer Fawns With Either A Bvdv1 Or A Bvdv2 Strain, Julia F. Ridpath, C. Scott Mark, Christopher C.L. Chase, Alanson C. Ridpath, John D. Neill

Veterinary and Biomedical Sciences Faculty Publications

Although commonly associated with infection in cattle, bovine viral diarrhea viruses (BVDV) also replicate in many domestic and wildlife species, including cervids. Bovine viral diarrhea viruses have been isolated from a number of cervids, including mule deer (Odocoileus hemionus), European roe deer (Capreolus capreolus), red deer (Cervus elaphus), white-tailed deer (Odocoileus virginianus), and mouse deer (Tragulus javanicus), but little information is available regarding clinical presentation and progression of infection in these species. In preliminary studies of experimental infection of deer with BVDV, researchers noted seroconversion but no clinical signs. In this study, we infected white-tailed deer fawns that were negative …

Otk18, A Zinc-Finger Protein, Regulates Human Immunodeficiency Virus Type 1 Long Terminal Repeat Through Two Distinct Regulatory Regions, Masahide Horiba, Lindsey B. Martinez, James L. Buescher, Sinji Sato, Jenae Limoges, Yunquan Jiang, Clinton J. Jones, Tsuneya Ikezu

School of Veterinary and Biomedical Sciences: Faculty Publications

It has previously been shown by our laboratory that OTK18, a human immunodeficiency virus (HIV)-inducible zinc-finger protein, reduces progeny-virion production in infected human macrophages. OTK18 antiviral activity is mediated through suppression of Tat-induced HIV-1 long terminal repeat (LTR) promoter activity. Through the use of LTR scanning mutant vectors, the specific regions responsible for OTK18-mediated LTR suppression have been defined. Two different LTR regions were identifi ed as potential OTK18-binding sites by an enhanced DNA–transcription factor ELISA system; the negative-regulatory element (NRE) at –255/–238 and the Ets-binding site (EBS) at –150/–139 in the LTR. In addition, deletion of the EBS in …

Characterization Of The Equine 2'-5' Oligoadenylate Synthetase 1 (Oas1) And Ribonuclease L (Rnasel) Innate Immunity Genes, Jonathan J. Rios, Andrey A. Perelygin, Maureen T. Long, Teri L. Lear, Andrey A. Zharkikh, Margo A. Brinton, David L. Adelson

Veterinary Science Faculty Publications

BACKGROUND: The mammalian OAS/RNASEL pathway plays an important role in antiviral host defense. A premature stop-codon within the murine Oas1b gene results in the increased susceptibility of mice to a number of flaviviruses, including West Nile virus (WNV). Mutations in either the OAS1 or RNASEL genes may also modulate the outcome of WNV-induced disease or other viral infections in horses. Polymorphisms in the human OAS gene cluster have been previously utilized for case-control analysis of virus-induced disease in humans. No polymorphisms have yet been identified in either the equine OAS1 or RNASEL genes for use in similar case-control studies.

RESULTS: …

Nociception In Fish: Stimulus–Response Properties Of Receptors On The Head Of Trout Oncorhynchus Mykiss, Paul J. Ashley, Lynne U. Sneddon, Catherine R. Mccrohan

Veterinary Science and Medicine Collection

This study examined stimulus–response properties of somatosensory receptors on the head of rainbow trout, Oncorhynchus mykiss, using extracellular recording from single cells in the trigeminal ganglion. Of 121 receptors recorded from 39 fish, 17 were polymodal nociceptors, 22 were mechanothermal nociceptors, 18 were mechanochemical receptors, 33 were fast adapting mechanical receptors and 31 were slowly adapting mechanical receptors. Mechanical thresholds were higher in polymodal nociceptors than in either slowly adapting or fast adapting mechanical receptors, whereas thermal thresholds of mechanothermal nociceptors were higher than those of polymodal nociceptors. Polymodal nociceptors and mechanochemical receptors gave similar responses to topical applications of …

Rapid Phase Adjustment Of Melatonin And Core Body Temperature Rhythms Following A 6-H Advance Of The Light/Dark Cycle In The Horse, Barbara A. Murphy, Jeffrey A. Elliott, Dawn R. Sessions, Mandi M. Vick, Erin L. Kennedy, Barry P. Fitzgerald

Veterinary Science Faculty Publications

BACKGROUND: Rapid displacement across multiple time zones results in a conflict between the new cycle of light and dark and the previously entrained program of the internal circadian clock, a phenomenon known as jet lag. In humans, jet lag is often characterized by malaise, appetite loss, fatigue, disturbed sleep and performance deficit, the consequences of which are of particular concern to athletes hoping to perform optimally at an international destination. As a species renowned for its capacity for athletic performance, the consequences of jet lag are also relevant for the horse. However, the duration and severity of jet lag related …

Isotype-Specific Antibody Responses Against Escherichia Coli O157:H7 Locus Of Enterocyte Effacement Proteins In Adult Beef Cattle Following Experimental Infection, G. Bretschneider, E. M. Berberov, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Escherichia coli O157:H7 is an important food-borne pathogen and cause of hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are an important reservoir of E. coli O157:H7, in which the organism colonizes the intestinal tract and is shed in the feces. Vaccination of cattle has significant potential as a pre-harvest intervention strategy for E. coli O157:H7; however, basic information about the bovine immune responses to important bacterial colonization factors resulting from infection has not been reported. The serum and fecal IgG and IgA antibody responses of adult cattle to E. coli O157:H7 intimin, translocated intimin receptor (Tir), E. coli …

Nucleic Acids Encoding Sarcocystis Neurona Antigen And Uses Thereof, Daniel K. Howe

Veterinary Science Faculty Patents

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from Sarcocystis neurona, or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG4. Also provided are isolated nucleic acids capable of selectively hybridizing with the nucleic acid from Sarcocystis neurona. The invention also provides vectors comprising the nucleic acids of the invention encoding an antigenic protein derived from Sarcocystis neurona or a …

Low Molecular Weight Protein Tyrosine Phosphatase (Lmw-Ptp) And Its Possible Physiological Functions Of Redox Signaling In The Eye Lens, Kuiyi Xing, Ashraf Raza, Stefan Lofgren, M. Rohan Fernando, Ye-Shih Ho, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Low molecular weight protein tyrosine phosphatase (LMW-PTP) was cloned from human lens epithelial B3 cells (HLE B3) and the recombinant enzyme was purified to homogeneity. The pure enzyme reacted positively with anti-LMW-PTP antibody, displayed tyrosine-specific phosphatase activity and was extremely sensitive to H2O2. The inactivated LMW-PTP could be regenerated by thioltransferase (TTase)/GSH system as demonstrated by both activity assay and by mass spectrometry (MS). The MS study also showed that an intramolecular disulfide bond was formed between C13 and C18 at the active site, and was reduced by the TTase/GSH system. The putative role of LMW-PTP in regulating platelet derived …

Ethical Issues In Geriatric Feline Medicine, Bernard E. Rollin

Veterinary Science and Medicine Collection

Most veterinarians hold a ‘pediatric’ rather than ‘garage mechanic’ view of their function. In recent years, sophisticated medical modalities have allowed veterinarians to keep animals alive, and increased value of companion animals in society has increased demand for such treatment. But whereas humans can choose to trade current suffering for extended life, animals seem to lack the cognitive apparatus required to do so. Thus, veterinarians must guard against keeping a suffering animal alive for too long. Clients may be emotionally tied to the animal and blind to its suffering. Part of the veterinarian’s role, therefore, is to lead the client …

Sheep Updates 2007 - Contents, Department Of Agriculture And Food, Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2007 - Part 5, Bindi Murray, John Karlsson, Johan Greeff, Carolina Viñoles Gil, B. L. Paganoni, K. M. M. Glover, J. T. B. Milton, G. B. Martin, Hugh Dove, Bill Mckiernan, John Wilkins, Paul Greenwood, Linda Cafe

Sheep Updates

This session covers six papers from different authors:

GENETIC IMPROVEMENT

1. Breech Strike Resistance: Selecting for resistance traits reduces breech strike, Bindi Murray, John Karlsson, Johan Greeff, Department of Agriculture and Food, Western Australia

2. Breeding Merino Sheep for Worm Resistance increases profit in a Mediterranean Environment, John Karlsson and Johan Greeff Department of Agriculture and Food, Western Australia

FEEDING

3. Embryo lasses were not increased when Merino ewes that had lost weight were supplemented with lupins, C. Viñoles Gil, B.L. Paganoni, K.M.M. Glover, J.T.B. Milton & G.B. Martin, School of Animal Biology, Faculty of Natural and Agricultural Sciences, …

Sheep Updates 2007 - Part 3, Andrew Ritchie, Edward Riggall, James Hall, Gus Rose, Johan Greeff, John Young, M. Alchin, M. Young, T. Johnson, John Lucy, Martin Staines, Tim Wiley, Rob Grima, Sandra Prosser, Matt Ryan, Geoff Moore, Tony Albertsen, Phil Barrett-Lennard, George Woolston, John Titerington, Sarah Knight, Brianna Peake

Sheep Updates

This session covers seven papers from different authors:

PROFITABILITY

1. Benchmarking demonstrates both the potential and realised productivity gains in the sheep and wool industry, Andrew Ritchie, Edward Riggall and James Hall, ICON Agriculture, Darkan

2. Improving sheep genetics will increase farm profitability, Gus Rose, Johan Greeff Department of Agriculture and Food Western Australia, John Young Farming Systems Analysis Service, WA

3. Meat, Merinos and making money in WA Pastoral Zone, M. Alchin, M. Young and T. Johnson, Department of Agriculture and Food Western Australia,

GRAZING

4. Nitrogen - farmers' friend or foe? John Lucy and Martin Staines, Department …

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …

The Veterinary Profession’S Duty Of Care In Response To Disasters And Food Animal Emergencies, Kenneth E. Nusbaum, Bernard E. Rollin, James S. Wohl

Professional Veterinary Ethics Collection

No abstract provided.

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

[Page] 1- Head/Director's Message Diagnostic News [Page] 1- Classical Swine Fever Surveillance (Hog Cholera) at the SDSU ADRDL [Page] 2- Biopsy Service Changes at the ADRDL – Effective July 1, 2007 [Page] 2- Listing of Pharmacies Offering Compounding Services for Veterinarians – April 2007 [Page] 2- Holiday Hours Research News [Page] 3- CIDRV Research Looks at New HIV Drug [Page] 4- Research Spotlight: Dr. Ying Fang Extension News [Page] 5- National Animal Health Monitoring System (NAHMS) to Study Beef Industry in 2007-08 [Page] 6- Pieces and Parts Student News [Page] 7- SDSU Pre-Veterinary Students Accepted to Veterinary School [Page] 7- …

Cetacean Brains: How Aquatic Are They?, Lori Marino

Anatomy Collection

The adaptation of cetaceans to a fully aquatic lifestyle represents one of the most dramatic transformations in mammalian evolutionary history. Two of the most salient features of modern cetaceans are their fully aquatic lifestyle and their large brains. This review article will offer an overview of comparative neuroanatomical research on aquatic mammals, including analyses of odontocete cetacean, sirenian, pinniped, and fossil archaeocete brains. In particular, the question of whether a relationship exists between being fully aquatic and having a large brain is addressed. It has been hypothesized that the large, well‐developed cetacean brain is a direct product of adaptation to …

Cetaceans Have Complex Brains For Complex Cognition, Lori Marino, Richard C. Connor, R. Ewan Fordyce, Louis M. Herman, Patrick R. Hof, Louis Lefebvre, David Lusseau, Brenda Mccowan, Esther A. Nimchinsky, Adam A. Pack, Luke Rendell, Joy S. Reidenberg, Diana Reiss, Mark D. Uhen, Estel Van Der Gucht, Hal Whitehead

Anatomy Collection

We believe that the time is ripe to present an integrated view of cetacean brains, behavior, and evolution based on the wealth of accumulated and recent data on these topics. Our conclusions support the more generally accepted view that the large brain of cetaceans evolved to support complex cognitive abilities.

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Risk Factors Of Gastrointestinal Nematode Parasite Infections In Small Ruminants Kept In Smallholder Mixed Farms In Kenya, Agricola Odoi, Joseph M. Gathuma, Charles K. Gachuiri, Amos Omore

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Background

Helminth infections in small ruminants are serious problems in the developing world, particularly where nutrition and sanitation are poor. This study investigated the burden and risk factors of gastrointestinal nematode parasite infections in sheep and goats kept in smallholder mixed farms in the Kenyan Central Highlands. Three hundred and seven small ruminants were sampled from 66 smallholder mixed farms in agro-ecological zones 1 (humid) and 3 (semi-humid) in the Kenyan Central highlands. The farms were visited once a month for eight months during which a health and production survey questionnaire was administered. Fecal samples were collected at each visit …