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Articles 31 - 45 of 45
Full-Text Articles in Medicine and Health Sciences
Michigan Bovine Tuberculosis Eradication Project: 2005 Activities Report And Conference Proceedings
Michigan Bovine Tuberculosis Eradication Project: 2005 Activities Report And Conference Proceedings
Michigan Bovine Tuberculosis Bibliography and Database
On June 7 and 8, 2005, the State of Michigan and U.S. Department of Agriculture hosted the ninth annual bovine Tuberculosis (TB) meeting of scientists, with the intent to share research information and provide updates on policies, regulations and activities regarding bovine TB.
This document is a thank you to stakeholders and serves as an annual report offering insight into the finer points of this multi-agency project. The year 2005 has been one of milestones. The Upper Peninsula received TB-Free Status; only one TB positive farm was found during routine surveillance testing; and the disease prevalence rate in Deer Management …
Mycobacterium Avium Subsp. Paratuberculosis From Free-Ranging Deer And Rabbits Surrounding Minnesota Dairy Herds, Eran A. Raizman, Scott J. Wells, Peter A. Jordan, Glenn D. Delgiudice, Russell R. Bey
Mycobacterium Avium Subsp. Paratuberculosis From Free-Ranging Deer And Rabbits Surrounding Minnesota Dairy Herds, Eran A. Raizman, Scott J. Wells, Peter A. Jordan, Glenn D. Delgiudice, Russell R. Bey
Michigan Bovine Tuberculosis Bibliography and Database
The objectives of this study were to estimate the prevalence of Mycobacterium avium subsp. paratuberculosis (MAP) among deer and rabbits surrounding infected and noninfected Minnesota dairy farms using fecal culture, and to describe the frequency that farm management practices were used that could potentially lead to transmission of infection between these species. Fecal samples from cows and the cow environment were collected from 108 Minnesota dairy herds, and fecal pellets from free-ranging white-tailed deer and eastern cottontail rabbits were collected from locations surrounding 114 farms; all samples were tested using bacterial culture. In addition, a questionnaire was administered to 114 …
Bovine Tuberculosis Eradication: Uniform Methods And Rules, Effective January 1, 2005
Bovine Tuberculosis Eradication: Uniform Methods And Rules, Effective January 1, 2005
Michigan Bovine Tuberculosis Bibliography and Database
These Uniform Methods and Rules (UM&R) are the minimum standards adopted and approved by the Deputy Administrator, Veterinary Services (VS), Animal and Plant Health Inspection Service (APHIS), on January 20, 2005. They were established for the maintenance of tuberculosis-free accredited herds of cattle and bison and the maintenance of State or zone status in the U.S. Department of Agriculture’s (USDA) tuberculosis eradication program.
These minimum standards do not preclude the adoption of more stringent standards by any State or zone.
Department Of Veterinary And Biomedical Sciences: 2005 Annual Report
Department Of Veterinary And Biomedical Sciences: 2005 Annual Report
Veterinary and Biomedical Sciences Department: Information and History
Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program - Courses 2005
VBMS Grants and Contracts Program, 2005
Publications by VBMS Faculty in 2005
Selected Committees, Editorial and Other Appointments. 2005
Articles Regarding the Department in 2005
Departmental Budget Summaries, 2005
Nebraska Agricultural Statistics, 2004/2005.
Chronic Wasting Disease Of Elk: Transmissibility To Humans Examined By Transgenic Mouse Models, Qingzhong Kong, Shenghai Huang, Wenquan Zou, Difernando Vanegas, Meiling Wang, Di Wu, Jue Yuan, Mengjie Zheng, Hua Bai, Huayun Deng, Ken Chen, Allen L. Jenny, Katherine I. O'Rourke, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Pertersen, Man-Sun Sy, Shu G. Chen, Pierluigi Gambetti
Chronic Wasting Disease Of Elk: Transmissibility To Humans Examined By Transgenic Mouse Models, Qingzhong Kong, Shenghai Huang, Wenquan Zou, Difernando Vanegas, Meiling Wang, Di Wu, Jue Yuan, Mengjie Zheng, Hua Bai, Huayun Deng, Ken Chen, Allen L. Jenny, Katherine I. O'Rourke, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Pertersen, Man-Sun Sy, Shu G. Chen, Pierluigi Gambetti
Zoonotics and Wildlife Disease: Publications
Chronic wasting disease (CWD), a prion disease affecting free-ranging and captive cervids (deer and elk), is widespread in the United States and parts of Canada. The large cervid population, the popularity of venison consumption, and the apparent spread of the CWD epidemic are likely resulting in increased human exposure to CWD in the United States. Whether CWDis transmissible to humans, as has been shown for bovine spongiform encephalopathy (the prion disease of cattle), is unknown. We generated transgenic mice expressing the elk or human prion protein (PrP) in a PrP-null background. After intracerebral inoculation with elk CWD prion, two lines …
Experimental Transmission Of Chronic Wasting Disease Agent From Mule Deer To Cattle By The Intracerebral Route, Amir N. Hamir, Robert A. Kunkle, Randall C. Cutlip, Janice M. Miller, Katherine I. O'Rourke, Elizabeth S. Williams, Michael W. Miller, Mick J. Stack, Melanie J. Chaplin, Jügen A. Richt
Experimental Transmission Of Chronic Wasting Disease Agent From Mule Deer To Cattle By The Intracerebral Route, Amir N. Hamir, Robert A. Kunkle, Randall C. Cutlip, Janice M. Miller, Katherine I. O'Rourke, Elizabeth S. Williams, Michael W. Miller, Mick J. Stack, Melanie J. Chaplin, Jügen A. Richt
Zoonotics and Wildlife Disease: Publications
This communication reports final observations on experimental transmission of chronic wasting disease (CWD) from mule deer to cattle by the intracerebral route. Thirteen calves were inoculated intracerebrally with brain suspension from mule deer naturally affected with CWD. Three other calves were kept as uninoculated controls. The experiment was terminated 6 years after inoculation. During that time, abnormal prion protein (PrPres) was demonstrated in the central nervous system (CNS) of 5 cattle by both immunohistochemistry and Western blot. However, microscopic lesions suggestive of spongiform encephalopathy (SE) in the brains of these PrPres-positive animals were subtle in 3 …
Predictive Spatial Dynamics And Strategic Planning For Raccoon Rabies Emergence In Ohio, James E. Childs, David L. Smith, Colin A. Russell, Leslie A. Real
Predictive Spatial Dynamics And Strategic Planning For Raccoon Rabies Emergence In Ohio, James E. Childs, David L. Smith, Colin A. Russell, Leslie A. Real
Zoonotics and Wildlife Disease: Publications
Rabies is an important public health concern in North America because of recent epidemics of a rabies virus variant associated with raccoons. The costs associated with surveillance, diagnostic testing, and post-exposure treatment of humans exposed to rabies have fostered coordinated efforts to control rabies spread by distributing an oral rabies vaccine to wild raccoons. Authorities have tried to contain westward expansion of the epidemic front of raccoonassociated rabies via a vaccine corridor established in counties of eastern Ohio, western Pennsylvania, and West Virginia. Although sporadic cases of rabies have been identified in Ohio since oral rabies vaccine distribution in 1998, …
Evaluation Of Western Blotting Methods Using Samples With Or Without Sodium Phosphotungstic Acid Precipitation For Diagnosis Of Scrapie And Chronic Wasting Disease, Hongsheng Huang, Jasmine Rendulich, Dan Stevenson, Katherine I. O'Rourke, Aru Balachandran
Evaluation Of Western Blotting Methods Using Samples With Or Without Sodium Phosphotungstic Acid Precipitation For Diagnosis Of Scrapie And Chronic Wasting Disease, Hongsheng Huang, Jasmine Rendulich, Dan Stevenson, Katherine I. O'Rourke, Aru Balachandran
Zoonotics and Wildlife Disease: Publications
The purpose of this study was to enhance the sensitivity of the Western blot (WB) test for use as an alternative and confirmatory method for the diagnosis of scrapie and chronic wasting disease (CWD) in Canada by comparing 2 sample preparation procedures: an abnormal prion protein (PrPSc) concentration procedure using sodium phosphotungstic acid (PTA) precipitation and a procedure using crude sample without precipitation. A total of 100 cerebrum samples (52 sheep and 48 elk), including 66 negative (31 sheep, 35 elk) and 34 positive (21 scrapie and 13 CWD positive) samples diagnosed by using immunohistochemistry (IHC) on retropharyngeal …
The Incidence Of Genotypes At Codon 171 Of The Prion Protein Gene (Prnp) In Five Breeds Of Sheep And Production Traits Of Ewes Associated With Those Genotypes, B. M. Alexander, R. H. Stobart, W. C. Russell, Katherine I. O'Rourke, G. S. Lewis, J. R. Logan, J. V. Duncan, G. E. Moss
The Incidence Of Genotypes At Codon 171 Of The Prion Protein Gene (Prnp) In Five Breeds Of Sheep And Production Traits Of Ewes Associated With Those Genotypes, B. M. Alexander, R. H. Stobart, W. C. Russell, Katherine I. O'Rourke, G. S. Lewis, J. R. Logan, J. V. Duncan, G. E. Moss
Zoonotics and Wildlife Disease: Publications
Scrapie is one of several transmissible spongiform encephalopathies of livestock. Disease susceptibility is linked to polymorphisms in the normal prion protein gene that encodes the mammalian prion precursor. Codon 171 of this gene is a major determinant of scrapie susceptibility. Selection for arginine (R) at codon 171 is encouraged by the USDA to decrease the incidence of scrapie. Objectives of this study were to determine the frequency of R allele variants at codon 171 in a sample of sheep from five breeds (Columbia, Hampshire, Rambouillet, Suffolk, and Targhee) and western white-faced commercial ewes and to determine whether the R allele …
Wildlife-Cattle Interactions In Northern Michigan: Implications For The Transmission Of Bovine Tuberculosis, Jerry Alan Hill
Wildlife-Cattle Interactions In Northern Michigan: Implications For The Transmission Of Bovine Tuberculosis, Jerry Alan Hill
Michigan Bovine Tuberculosis Bibliography and Database
Bovine tuberculosis (Mycobacterium bovis) was discovered in northern Michigan white-tailed deer (Odocoileus virginianus) in 1994, and has been known to exist in Michigan cattle herds since 1998. Despite efforts to eradicate the disease in cattle, infection and re-infection of farms continues to occur, suggesting transmission among cattle, deer, or other wildlife reservoirs. The goals of this study were to document wildlife activity on farms and evaluate the possible role wildlife play in the ecology of bovine tuberculosis (TB) in Michigan. Visual observations were conducted on farms in a 5-county area of northern Michigan to document direct …
Development And Pathogenesis Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, Eric P. Hoberg, L. Polley
Development And Pathogenesis Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, Eric P. Hoberg, L. Polley
Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications
Recently, the protostrongylid nematode Parelaphostrongylus odocoilei has been reported in a new host species, thinhorn sheep (Ovis dalli). For the first time, we completed the life cycle of P. odocoilei in three Stone’s sheep (O. dalli stonei) and two thinhorn hybrids (O. dalli stonei × O. dalli dalli), each infected with 200 third-stage larvae from slugs (Deroceras laeve). The prepatent period ranged from 68 days to 74 days, and shedding of first-stage larvae (L1) peaked at >10,000 L1 per gram of feces between 90 and 110 days postinfection. A total of 75, …
Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo
Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo
Jay Reddy Publications
Female B10.S mice are highly resistant to proteolipid protein (PLP) 139–151-induced experimental autoimmune encephalomyelitis (EAE) and depletion of PLP 139–151- reactive CD4+CD25+regulatory T (Treg) cells can slightly increase their EAE susceptibility. Although male B10.S mice are moderately susceptible to EAE, we report that depletion of Treg cells in male B10.S mice before immunization with PLP 139–151 renders them highly susceptible to severe EAE with more CNS neutrophil infiltrates than nondepleted controls. Increased susceptibility is associated with an enhanced PLP 139–151-specific T cell response and greater production of IFN-γ, IL-6, and IL-17. Male CD4+CD25+ effector cells depleted of Treg cells proliferate …
Latency-Related Gene Encoded By Bovine Herpesvirus 1 Promotes Virus Growth And Reactivation From Latency In Tonsils Of Infected Calves, Sandra Perez, Melissa Inman, Alan R. Doster, Clinton J. Jones
Latency-Related Gene Encoded By Bovine Herpesvirus 1 Promotes Virus Growth And Reactivation From Latency In Tonsils Of Infected Calves, Sandra Perez, Melissa Inman, Alan R. Doster, Clinton J. Jones
School of Veterinary and Biomedical Sciences: Faculty Publications
Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infected calves. Dexamethasone (DEX) consistently induces reactivation from latency, and viral gene expression is detected in TG and tonsils. In sensory neurons of latently infected calves, the latency-related (LR) gene is abundantly expressed and is required for reactivation from latency. In the present study, we compared the abilities of wild-type (wt) BHV-1 and a strain …
Serologic Survey Of Select Infectious Diseases In Coyotes And Raccoons In Nebraska, Richard Bischof, Douglas G. Rogers
Serologic Survey Of Select Infectious Diseases In Coyotes And Raccoons In Nebraska, Richard Bischof, Douglas G. Rogers
School of Veterinary and Biomedical Sciences: Faculty Publications
To obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in Nebraska, sera were collected from 67 coyotes (Canis latrans) and 63 raccoons (Procyon lotor)) from November 2002 through January 2003. For coyotes, antibodies were detected against canine distemper virus (CDV, 61%), Francisella tularensis) (32%), Rickettsia rickettsi) (13%), and flaviviruses (48%). None of the coyote sera had antibodies to Borrelia burgdorferi, Brucella canis), or six serovars of Leptospira interrogans). Because serologic cross-reactivity exists among flaviviruses, 14 sera from flavivirus-positive coyotes were also tested for St. Louis …
Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik
Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase and has multiple functions residing in its different domains. In the present study, we examined the role of the hypervariable hinge region of P protein in viral RNA synthesis and recovery of infectious VSV by using transposon-mediated insertion mutagenesis and deletion mutagenesis. We observed that insertions of 19-amino-acid linker sequences at various positions within this region affected replication and transcription functions of the P protein to various degrees. Interestingly, one insertion mutant was completely defective in both transcription and replication. Using …