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Full-Text Articles in Medicine and Health Sciences
The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert
The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert
The Thai Journal of Veterinary Medicine
The PRRS virus and PED alpha-coronavirus are the two main pig pathogens in the world. The current strategies to control these diseases are inadequate. The recombinant chimeric swine PKR-Apaf-1 protein (rcPAP) consists of the Human Immunodeficiency Virus Trans-activator transcription (HIV-TAT) domain, the dsRNA-binding domain of porcine Protein kinase R (PKR) gene and the caspase recruitment domain (CARD) domain of porcine Apoptotic Protease-Activating Factor-1 (Apaf-1) gene. In general, the rcPAP can detect viral dsRNA in virally infected cells and induces apoptosis and kills the virus-infected cells. In this study, the reducing viral-load efficacy of rcPAP (produced from bacteria) against PRRS virrus …
Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur
Monensin Induces Apoptosis In The Liver Tissue And Primary Hepatocytes Of Chicks, Muhammet Yasi̇n Tekeli̇, Murat Kanbur
Turkish Journal of Veterinary & Animal Sciences
In this study, monensin (MON) was investigated for its apoptotic and genotoxic effects on chick liver tissue and cytotoxic and apoptotic effects on chick primary hepatocytes isolated from perfused liver tissue. Western blotting and real-time PCR (qPCR) were used to determine the apoptotic effect on primary hepatocytes and liver tissue, and the comet assay was used to determine the genotoxic effect on liver tissue. MON decreased cell viability in primary hepatocytes with increasing concentrations. When administered at concentrations of 1 and 10 μM, MON increased the levels of apoptotic p53, caspase-3, and caspase-9, and downregulated the expression of the antiapoptotic …