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Full-Text Articles in Medicine and Health Sciences

Amplification Of The Matrix Gene Of Rbok Vaccine Strain Of Rinderpest Virus (Rpv) By Polymerase Chain Reaction And Cloning Into Topo^R Xl Cloning Vector, Ahmet Kürşat Azkur, Yusuf Bolat, Mehmet Zi̇ya Doymaz Jan 2003

Amplification Of The Matrix Gene Of Rbok Vaccine Strain Of Rinderpest Virus (Rpv) By Polymerase Chain Reaction And Cloning Into Topo^R Xl Cloning Vector, Ahmet Kürşat Azkur, Yusuf Bolat, Mehmet Zi̇ya Doymaz

Turkish Journal of Veterinary & Animal Sciences

In this study, the matrix (M) gene of the RBOK vaccine strain of rinderpest (RPV) was amplified by polymerase chain reaction (PCR) and cloned into TOPO^R XL cloning vector. For this purpose, Vero cells were infected with the RBOK vaccine strain of RPV and total RNA was obtained from the infected cells. cDNA of the matrix gene was obtained by reverse transcription from the total RNA. Amplification of the cDNA with PCR was achieved by using the M gene specific primer and PCR products of the M gene were directly ligated into the TOPO^R XL cloning vector and this recombinant …


The Effect Of Transcervical Intrauterine And Intravaginal Insemination Methods On Fertility In Dogs, Ali̇ Daşkin, Necmetti̇n Teki̇n, Ergun Akçay Jan 2003

The Effect Of Transcervical Intrauterine And Intravaginal Insemination Methods On Fertility In Dogs, Ali̇ Daşkin, Necmetti̇n Teki̇n, Ergun Akçay

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of transcervical and intravaginal artificial insemination on the pregnancy rate in dogs. Semen was collected by the massage method from dogs brought to our department. Collected semen was examined in the laboratory and stored in liquid nitrogen after dilution with Tris extender. During the study 31 bitches brought to our department for artificial insemination were used. One of the bitches was not evaluated. Oestrus was detected by vaginal cytology and the bitches were inseminated twice during the oestrus period. Fifteen of the 30 bitches were inseminated transcervically and the other 15 …


Isolation Of Klebsiella Pneumoniae From Mares With Metritis And Stallions, Detection Of Biotypes And Capsule Types, N. Yakut Özgür, Arzu Funda Bağcigi̇l, Serkan İki̇z, M. Ragip Kiliçarslan, Babür Cari̇oğlu, Ati̇lla Ilgaz Jan 2003

Isolation Of Klebsiella Pneumoniae From Mares With Metritis And Stallions, Detection Of Biotypes And Capsule Types, N. Yakut Özgür, Arzu Funda Bağcigi̇l, Serkan İki̇z, M. Ragip Kiliçarslan, Babür Cari̇oğlu, Ati̇lla Ilgaz

Turkish Journal of Veterinary & Animal Sciences

In 2000-2001, the frequency of metritis cases due to Klebsiella pneumoniae was investigated in Thoroughbred breeding mares in the Marmara region of Turkey, where most racehorses are reared. In addition, Thoroughbred stallions from the breeding centre in the İzmit district were examined for the presence of K. pneumoniae. Biotypes and capsule types of the isolates were detected, and their antibiotic susceptibilities were determined. Intrauterine swabs taken from 56 mares with metritis, and semen samples and swabs of urethral fossa from 27 stallions were investigated. K. pneumoniae was isolated from 13 (23.2%) mares and 2 (7.4%) stallions. Biotyping of the isolates …


The Use Of Recombinant Hemagglutinine Protein Of Rinderpest Virus In Enzyme Immunoassay, Hakan Bulut, Yusuf Bolat, Yasemi̇n Bulut, Aykut Özdarendeli̇, Mehmet Zi̇ya Doymaz Jan 2003

The Use Of Recombinant Hemagglutinine Protein Of Rinderpest Virus In Enzyme Immunoassay, Hakan Bulut, Yusuf Bolat, Yasemi̇n Bulut, Aykut Özdarendeli̇, Mehmet Zi̇ya Doymaz

Turkish Journal of Veterinary & Animal Sciences

In this study, Rinderpest virus (RPV) recombinant hemagglutinine protein (rH) fused with protein A region of Staphylococcus aureus was expressed in Escherichia coli and purified by IgG affinity chromatography. rH protein was also used to establish enzyme immunoassay. Therefore, to prevent IgG binding to the protein A the wells coated with the rH proteins were blocked by human serum. Afterwards, RPV antigens were added to the wells to evaluate this assay. To this end, serum from mice immunized with RPV was used for EIA. Our data indicated that the rH protein fused with protein A of Staphylococcus aureus can be …


Adherence And Slime Production In Coagulase Negative Staphylococci Isolated From Different Animal Sources, Oktay Keski̇n, Gülay Altay, Mehmet Akan Jan 2003

Adherence And Slime Production In Coagulase Negative Staphylococci Isolated From Different Animal Sources, Oktay Keski̇n, Gülay Altay, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine slime production and the adherence of coagulase negative staphylococci (CNS) strains isolated from cows' milk with mastitis and chickens which have pathological disorders. For this purpose, 60 CNS strains isolated from cows' milk with mastitis and 60 from chickens suffering from skin and joint diseases were examined. In addition, 20 strains isolated from healthy cows and healthy chickens were used as control groups. Slime production was conductet by the tube method and the results were recorded as -, +, ++ and +++ after 24 h incubation according to the formation of a …


Some Production Traits Of Karayaka And Bafra (Chios X Karayaka B_1) Sheep, Fati̇h Atasoy, Necmetti̇n Ünal, Hali̇l Akçapinar, Durhasan Mundan Jan 2003

Some Production Traits Of Karayaka And Bafra (Chios X Karayaka B_1) Sheep, Fati̇h Atasoy, Necmetti̇n Ünal, Hali̇l Akçapinar, Durhasan Mundan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate live weight, wool production and some body measurements of Karayaka and Bafra (Chios x Karyaka B_1) sheep. The Karayaka breed is raised in the Black Sea region, while the Sakız (Chios) breed is raised in the coastal areas of western Anatolia. The Bafra type was developed by crossing between Sakız and Karayaka for a female line to use in lamb production. The least squares means obtained for Karayaka and Bafra yearlings were determined to be 48.4 and 55.0 kg for live weight, 56.2 and 63.8 cm for wither height, 59.6 and 71.0 cm …


Fertility Traits, Survival Rate And Growth Characteristics Of Karayaka And Bafra (Chios X Karayaka B_1) Genotypes, Necmetti̇n Ünal, Fati̇h Atasoy, Hali̇l Akçapinar, Meti̇n Erdoğan Jan 2003

Fertility Traits, Survival Rate And Growth Characteristics Of Karayaka And Bafra (Chios X Karayaka B_1) Genotypes, Necmetti̇n Ünal, Fati̇h Atasoy, Hali̇l Akçapinar, Meti̇n Erdoğan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the fertility traits, survival rate and growth characteristics of Karayaka and Bafra (Chios x Karayaka B_1) genotypes. Karayaka and Bafra genotypes are raised in the Black Sea region of Turkey. In the study, fertility results for Karayaka and Bafra were determined to be 92.3 and 93.7% for birth rates, and 1.08 and 1.78 for litter size, respectively. The survival rates of Karayaka and Bafra lambs at weaning (90 days) were 93.6 and 91.9%, respectively. The least squares means obtained for Karayaka and Bafra lambs were 3.1 and 3.7 kg for birth weight, 19.5 …


The Effects Of Serotonin On The Motility Of Small And Large Intestine Of Guinea Pigs In Fasting, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Bahri̇ Emre, Elvi̇n Akdağ Jan 2003

The Effects Of Serotonin On The Motility Of Small And Large Intestine Of Guinea Pigs In Fasting, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Bahri̇ Emre, Elvi̇n Akdağ

Turkish Journal of Veterinary & Animal Sciences

This study was carried out in vitro to observe the effects of serotonin (5-HT), identified as a transmitter candidate for the regulation of intestinal motility, on the motility of the small and large intestines of fed and fasted guinea pigs. In the study, 48 adult guinea pigs weighing 300-450 g were divided equally into control (fed) and experimental (fasted for 96 h) groups. Half of the animals in both groups were used for taking small intestinal segments, the mid jejunum and distal ileum, while the rest of the animals were used to take large intestinal segments, as well as proximal …


A Study On The Chromosomes Of Konya Wild Sheep (Ovis Orientalis Spp.): Case Report, Kemal Kirikçi, Ayşegül Zamani̇, Gül Durakbaşi Jan 2003

A Study On The Chromosomes Of Konya Wild Sheep (Ovis Orientalis Spp.): Case Report, Kemal Kirikçi, Ayşegül Zamani̇, Gül Durakbaşi

Turkish Journal of Veterinary & Animal Sciences

We investigated the shape and number of chromosomes of Konya wild sheep. A karyotype was prepared from G-band painted chromosomes. Konya wild sheep have 54 diploid chromosomes. The first three autosomal chromosomes were metacentric, and the other autosomal chromosomes and X chromosomes were acrocentric.


Pathological And Immunohistochemical Studies In Rabbits Experimentally Infected With Toxoplasma Gondii, Rifki Haziroğlu, Kürşat Altintaş, Ayhan Atasever, M. Yavuz Gülbahar, Oğuz Kul, Recai̇ Tunca Jan 2003

Pathological And Immunohistochemical Studies In Rabbits Experimentally Infected With Toxoplasma Gondii, Rifki Haziroğlu, Kürşat Altintaş, Ayhan Atasever, M. Yavuz Gülbahar, Oğuz Kul, Recai̇ Tunca

Turkish Journal of Veterinary & Animal Sciences

Experimental toxoplasmosis was induced in rabbits using different routes. A total of 42 animals were used; 36 of them were in the experimental groups and the other six comprised the control group. In addition, five guinea pigs were used for preparing hyperimmune sera against Toxoplasma gondii. The Ankara strain was inoculated intravenously (group I, n: 12), intraperitoneally (group II, n: 12) and intradermally (group III, n: 12) into the rabbits. Group IV animals were kept as controls (n: 6). Clinical and macroscopical findings varied according to the routes of inoculation and organs affected. Microscopically, multifocal necrosis with T. gondii tachyzoites …


Lipid And Lipoprotein Levels In Dairy Cows With Fatty Liver, Mutlu Sevi̇nç, Abdullah Başoğlu, Hasan Güzelbektaş, Murat Boydak Jan 2003

Lipid And Lipoprotein Levels In Dairy Cows With Fatty Liver, Mutlu Sevi̇nç, Abdullah Başoğlu, Hasan Güzelbektaş, Murat Boydak

Turkish Journal of Veterinary & Animal Sciences

The purpose of this investigation was to establish whether there were any differences in the lipid and lipoprotein levels of dairy cows with fatty liver. Fifty-four dairy cows with fatty liver and 12 healthy dairy cows were used in the study. The liver fat content was determined in all cows histologically. Cows with fatty liver (n = 54) were grouped according to liver fat content as cows with mild (n = 17), moderate (n = 17) or severe(n = 20) fatty liver. Fat infiltration in the liver was not observed in the healthy group of cows. Some serum chemical parameters …


Controlling The Breeding Season Using Melatonin And Progestagen In Kıvırcık Ewes, Huri̇ye Horoz Kaya, Güven Kaşikçi, Kemal Ak, Serhat Alkan, Cumhur Sönmez Jan 2003

Controlling The Breeding Season Using Melatonin And Progestagen In Kıvırcık Ewes, Huri̇ye Horoz Kaya, Güven Kaşikçi, Kemal Ak, Serhat Alkan, Cumhur Sönmez

Turkish Journal of Veterinary & Animal Sciences

The effect of melatonin and progestagen sponge administrations separately and together on the reproductive performance at the onset of the breeding season were investigated in Kıvırcık ewes. On June 22, to 40 anoestrous and non-lactating ewes were administrated melatonin (Regulin) implants. On July 14, progestagen (Synchron) sponges were inserted in 20 of these ewes and in 20 other ewes. They were removed 14 days later and on the day of removal the ewes were given 600 I.U. pregnant mare's serum gonadotrophin (PMSG). The other 20 ewes served as controls. Rams were introduced on July 28. The lambing percentages of the …


The Efficacy Of Moxidectin And Doramectin Against Gastrointestinal Nematode Infection In Cattle, Erol Ayaz, Ali̇ Şahi̇n Jan 2003

The Efficacy Of Moxidectin And Doramectin Against Gastrointestinal Nematode Infection In Cattle, Erol Ayaz, Ali̇ Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

The efficacy of doramectin (Dectomax, Pfizer) and moxidectin (Cydectin, Abfar) against gastrointestinal nematode infection was evaluated in cows which were naturally infected with these nematodes. For that purpose over 100 cows' faeces were tested for gastrointestinal nematodes with the saturated salt solution method. Infected cows were selected according to the McMaster criteria. To establish whether the cows were infected with gastrointestinal nematodes, their coprocultures were screened for the presence of Ostertagia, Haemonchus, Nematodirus and Trichostrongylus species. Thirty cows which had acquired nematodes naturally were allocated to three groups of 10. The cows in the first and second group were administered …


Comparison Of Fatty Acid Profiles Of Different Tissues Of Mature Trout (Salmo Trutta Labrax, Pallas, 1811) Caught From Kazandere Creek In The Çoruh Region, Erzurum, Turkey, N. Mevlüt Aras, H. İbrahi̇m Hali̇loğlu, Özer Ayik, Hasan Yeti̇m Jan 2003

Comparison Of Fatty Acid Profiles Of Different Tissues Of Mature Trout (Salmo Trutta Labrax, Pallas, 1811) Caught From Kazandere Creek In The Çoruh Region, Erzurum, Turkey, N. Mevlüt Aras, H. İbrahi̇m Hali̇loğlu, Özer Ayik, Hasan Yeti̇m

Turkish Journal of Veterinary & Animal Sciences

The fatty acid (FA) composition of some tissues of Salmo trutta labrax, Pallas, 1811 mature native freshwater wild salmon species caught from Kazandere creek in the Çoruh region in Turkey were investigated. Fatty acids profiles of muscle, liver, gonad, egg and adipose tissue were compared in terms of total and individual saturated and unsaturated fatty acids. The results showed that the highest total saturated fatty acid content was in the muscle (37.20 ± 1.13%) while the lowest value was in the egg (27.12 ± 2.53%), and the differences were significant (P < 0.05) among the tissues. Monounsaturated fatty acid contents (MUFA) were not significantly different (P > 0.05) for the tissues but n-3 polyunsaturated fatty acid …


Investigation Of Some Population Parameters Of Common Sole, Solea Solea (L., 1758) From İskenderun Bay, Mustafa Türkmen Jan 2003

Investigation Of Some Population Parameters Of Common Sole, Solea Solea (L., 1758) From İskenderun Bay, Mustafa Türkmen

Turkish Journal of Veterinary & Animal Sciences

Data on age, growth, reproduction, mortality and exploitation were examined for common sole collected in İskenderun Bay from March 2000 to February 2001. The examined individuals were aged between 1 and 8 years. Total length ranged from 8.8 to 28.2 cm. The length-weight relationships for males (a = 0.0117, b = 2.998) and females (a = 0.0091, b = 3.077) showed no significant difference from isometric growth. Von Bertalanffy growth parameters were L_{\infty} = 26.03 cm, k = 0.221 year^{-1}, t_o = -1.31 for males and L_{\infty} = 29.95 cm, k = 0.181 year^{-1}, t_o = -1.55 for females. The …


The Microbiological Quality Of Çiğ Köfte Sold In Ankara, Özlem Küplülü, Belgi̇n Sarimehmetoğlu, Nebahat Oral Jan 2003

The Microbiological Quality Of Çiğ Köfte Sold In Ankara, Özlem Küplülü, Belgi̇n Sarimehmetoğlu, Nebahat Oral

Turkish Journal of Veterinary & Animal Sciences

Çiğ köfte is a special Turkish meal prepared from minced meat, bulgur, onions, garlic and different spices. In this study, a total of 50 çiğ köfte samples sold in Ankara were microbiologically analysed in order to determine their microbiological quality. The numbers of total mesophilic aerobic bacteria, lactobacilli, micrococci/staphylococci, coagulase-positive staphylococci, enterobacteriaceae, coliform, enterococci, pseudomonas, yeasts and molds were determined by drop plating, whereas the B. cereus count was determined by spread plating, and the isolation of Salmonella involved enrichment procedures. The mean numbers of total mesophilic aerobic bacteria, lactobacilli, and micrococci/staphylococci were 10^6 cfu/g, 10^5 cfu/g, 10^4 cfu/g, respectively. …


Growth And Carcass Characteristics Of Three Fat-Tailed Pure Breeds Under Grazing With Concentrate Supplementation, Muhli̇s Maci̇t, Sirri Şahi̇n, Nuri̇ni̇sa Esenbuğa, Mevlüt Karaoğlu Jan 2003

Growth And Carcass Characteristics Of Three Fat-Tailed Pure Breeds Under Grazing With Concentrate Supplementation, Muhli̇s Maci̇t, Sirri Şahi̇n, Nuri̇ni̇sa Esenbuğa, Mevlüt Karaoğlu

Turkish Journal of Veterinary & Animal Sciences

Growth performance, carcass measurements and carcass characteristics of three fat-tailed Turkish breeds (Awassi = A, Morkaraman = M and Tushin = T) weaned at 2.5 months of age and subjected to semi-intensive feeding during a grazing period on pasture for 70 days were evaluated. At the end of the 70-day grazing period, M lambs showed the highest performance for daily weight gain and the amount of concentrate consumed per bodyweight gain in addition to grazing on pasture. The effects of the breed and sex of lambs on daily weight gain on pasture were significant (P < 0.05) and highly significant (P < 0.01), respectively. Male lambs were superior to female lambs with respect to daily weight gain on pasture. The slaughter traits, carcass measurements and carcass characteristics of 14 male lambs representing three fat-tailed breeds were compared at the end of the grazing period of the year in which they were born. After slaughter, carcasses were chilled at +4 °C for 24 h and measurements were taken on the intact cold carcasses. The carcasses were then dissected into wholesale cuts. The hot carcass weight and hindshank weight of M lambs were heavier than those of A and T lambs. M had a higher hot dressing percentage and larger M. longissimus dorsi (LD) area than those of A and T lambs. Most of the weights of wholesale cuts such as foreshank and breast, sirloin, leg, rib and hindshank were significantly (P < 0.05; P < 0.01) affected by sheep breed. It is recommended either that A, M or T male and female lambs weaned at 2.5 months of age in eastern Turkey should be fed with 450 g concentrate per head per day on pasture for 3 to 3.5 months of grazing season, or lambs should be supplemented with more than 450 g concentrate feed on pasture because native fat-tailed Turkish breeds supplemented with 450 g concentrate on pasture did not give carcasses with an adequate commercial weight at the end of a 70-day fattening period.


Effects Of Hypertonic Sodium Chloride, Hypertonic Sodium Chloride + Sodium Bicarbonate And Hypertonic Sodium Chloride + Ringer's Lactade Solution In The Treatment Of Dogs With Experimentally Induced Endotoxaemia, Hasan Batmaz, Zeki̇ Yilmaz, Ayşe Topal, O. Saci̇t Görgül, Sezgi̇n Şentürk Jan 2003

Effects Of Hypertonic Sodium Chloride, Hypertonic Sodium Chloride + Sodium Bicarbonate And Hypertonic Sodium Chloride + Ringer's Lactade Solution In The Treatment Of Dogs With Experimentally Induced Endotoxaemia, Hasan Batmaz, Zeki̇ Yilmaz, Ayşe Topal, O. Saci̇t Görgül, Sezgi̇n Şentürk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the effects of isotonic sodium chloride (ISS-0.9%), hypertonic sodium chloride (HSS-7.2%), HSS + lactate ringer solution (LRS) and HSS + sodium bicarbonate solution (SBS-1.3%) in the treatment of dogs with endotoxaemia. Endotoxaemia was induced by slow injection of a 1 mg/kg/i.v. dose of E. coli endotoxin (0111:B4). At the treatment stage, four groups, each composed of six dogs, were formed and a different treatment plan was carried out in each group: ISS, HSS, HSS + LRS and HSS + SBS. Clinical, haematological and biochemical examinations were performed before and after endotoxaemia. Hypotension …


Diagnostic Approach To The Prevalence Of Feline Periodontal Disease, Erdoğan Samsar, Ömer Beşalti, Ahmet Özak, Şükran Yavuzdemi̇r Jan 2003

Diagnostic Approach To The Prevalence Of Feline Periodontal Disease, Erdoğan Samsar, Ömer Beşalti, Ahmet Özak, Şükran Yavuzdemi̇r

Turkish Journal of Veterinary & Animal Sciences

Periodontal disease in cats was investigated with respect to clinical, radiological and microbiological evaluations. Each tooth group was evaluated according to plaque index, gingival index, sulcus bleeding index, calculus index, periodontal disease index, furcation exposure index, feline odontoclastic resorptive lesions (FORL) and tooth mobility. The prevalence of the disease was evaluated statistically. Upper and lower premolars were affected by periodontal disease at a higher rate than others. Tooth loss was often observed in incisives, premolars and canines concurrently. Sub and/or supragingival calculus accumulations occurred in lower incisive, upper canine and upper premolar teeth, in increasing order. FORL was observed in …


Morphological And Biophysical Properties Of The Cells Involving The Reflexive Orienting Responses Of The Eye, Head And Body, Ramazan Bal Jan 2003

Morphological And Biophysical Properties Of The Cells Involving The Reflexive Orienting Responses Of The Eye, Head And Body, Ramazan Bal

Turkish Journal of Veterinary & Animal Sciences

The intrinsic membrane properties of the neurons in the cortices of the inferior colliculus were studied in 300-µm transversal slices. The membrane properties, including the sub- and superthreshold, of the neurons were determined by intracellular stimulations with current injections. Of 49 intracellularly recorded neurons, 21 were successfully stained with intracellular biocytin injection. Morphologically all of the 21 neurons were identified as multipolar. Physiologically three firing patterns (onset, regular and adapting) were identified on the basis of response patterns to depolarizing current injections. Onset neurons had a non-linear, and regular and adapting neurons had linear current-voltage relationships. In conclusion, with the …


Expression Profiles Of Igf-I, Igf-Ii, Bfgf And Tgf-B2 Growth Factors During Chicken Embryonic Development, Hakan Kocamiş, John Killefer Jan 2003

Expression Profiles Of Igf-I, Igf-Ii, Bfgf And Tgf-B2 Growth Factors During Chicken Embryonic Development, Hakan Kocamiş, John Killefer

Turkish Journal of Veterinary & Animal Sciences

Skeletal muscle development in avian embryos depends on the activation, proliferation, differentiation, and fusion of embryonic myoblasts. These processes as well as early embryonic development are mainly regulated by major growth factors such as IGF-I, IGF-II, TGF-b and FGFs. In order to determine their differential expression, total RNA was isolated from whole embryos on each of the embryonal days (E) 0 to 6, from the thoracic/abdominal half of the embryo at E 7 and E 8, and from pectoralis muscle tissues at E 9 to E 20. Growth factor cDNAs were synthesized by reverse-transcription polymerase chain reaction (RT-PCR). All growth …


Pregnancy Diagnosis In Mares By Determination Of Oestradiol-17-B Hormone In Faeces, Muzaffer Çelebi̇, Mürsel Demi̇rel Jan 2003

Pregnancy Diagnosis In Mares By Determination Of Oestradiol-17-B Hormone In Faeces, Muzaffer Çelebi̇, Mürsel Demi̇rel

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was the confirmation of pregnancy diagnosis in mares by the determination of oestradiol-17 b in faeces. The research was carried out on 30 cyclic mares. Faeces samples were collected through pregnancy at monthly intervals. The faeces samples of pregnant and non-pregnant mares contained a mean oestradiol-17 b concentration of 9.39 ± 2.63 and 7.70 ± 2.00 ng/g, respectively in the 4^{th} month of gestation. From the 5^{th} month of pregnancy the oestradiol-17 b concentration in pregnant mares was significantly higher than in non-pregnant mares. Oestradiol-17 b levels were estimated to be 71.20 ± 8.00 …


Effects Of Medetomidine-Ketamine Combination Anaesthesia On Electrocardiographic Findings, Body Temperature, And Heart And Respiratory Rates In Domestic Pigeons, Metehan Uzun, Sedat Yildiz, Gülteki̇n Atalan, Mehmet Kaya, Nesri̇n Sulu Jan 2003

Effects Of Medetomidine-Ketamine Combination Anaesthesia On Electrocardiographic Findings, Body Temperature, And Heart And Respiratory Rates In Domestic Pigeons, Metehan Uzun, Sedat Yildiz, Gülteki̇n Atalan, Mehmet Kaya, Nesri̇n Sulu

Turkish Journal of Veterinary & Animal Sciences

The applications of medetomidine (M) as a pre-anaesthetic and ketamine (K) as an anaesthetic agent were investigated in seven domestic pigeons in terms of the degree of anaesthesia (DA) and alterations in physiological parameters. An intramuscular (IM) injection of M (200 µg/kg) was followed by administration of K (120 mg/kg, IM) after 10 min. Body temperature (BT), respiratory rate (RR), heart rate (HR), DA, and electrocardiogram (ECG) findings were determined before (0 min) and 10 min after the application of M, and 5, 15, 30, 45 and 60 min after the administration of K. All pigeons had 4^{th} degree anaesthesia …


A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan Jan 2003

A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan

Turkish Journal of Veterinary & Animal Sciences

Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …


Changes In Adenosine Nucleotides In The Heart Tissue Of Rats Following Different Types Of Death, Sadretti̇n Pençe, Hasan Koçoğlu, Naci̇ye Kurtul, Hali̇me Hanim Pençe, Cahi̇t Bağci Jan 2003

Changes In Adenosine Nucleotides In The Heart Tissue Of Rats Following Different Types Of Death, Sadretti̇n Pençe, Hasan Koçoğlu, Naci̇ye Kurtul, Hali̇me Hanim Pençe, Cahi̇t Bağci

Turkish Journal of Veterinary & Animal Sciences

Forty-eight Sprague-Dawley rats were killed by cervical dislocation, electric shock and drowning to investigate the correlations between types of death and levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in heart tissue after death. The hearts were taken out after death and ATP, ADP and AMP levels in the heart muscle were measured. The ATP and ADP levels were lower in rats killed by electric shock and with drowning. This may be due to ventricular fibrillation caused by electric shock in the electric shock group, and debilitation of rats in the drowning group before death, which …


Effect Of Low Birthweight On Serum Thyroid Hormones, Glucose, Urea And Blood Ph In Newborn Lambs, Tufan Keçeci̇ Jan 2003

Effect Of Low Birthweight On Serum Thyroid Hormones, Glucose, Urea And Blood Ph In Newborn Lambs, Tufan Keçeci̇

Turkish Journal of Veterinary & Animal Sciences

In this study, serum thyroid hormone, glucose and urea levels as well as blood pH values were compared between six control (birthweight ? 2.5 kg) and seven experimental Merino lambs (birthweight < 2.5 kg, hypotrophic) at 2, 4, 8 and 24 h postpartum. Serum levels of total thyroxin (T_4), free thyroxin (FT_4), total triiodothyronine (T_3; with exception of 24 h postpartum), free triiodothyronine (FT_3; with the exception of 4, 8 and 24 h postpartum) and glucose were lower in hypotrophic lambs compared to the controls. However, the serum urea levels of hypotrophic animals were higher than those of the controls. On the other hand, serum T_3, FT_3 and FT_4 levels determined at 4 and 8 h were higher than those at 2 and 24 h postpartum in both groups. A similar case was also observed in the serum T_4 levels of hypotrophic lambs. In addition, the serum glucose levels of hypotrophic lambs were higher and the blood pH values of both groups were lower at 2 h postpartum. In conclusion, It was observed that low birthweight affected neonatal thyroid function, serum glucose and urea levels in lambs.


Optimisation Of Uv Treatment Duration To Induce Haploid Androgenesis In The Nile Tilapia (Oreochromis Niloticus L.), İsmi̇han Karayücel, Sedat Karayücel Jan 2003

Optimisation Of Uv Treatment Duration To Induce Haploid Androgenesis In The Nile Tilapia (Oreochromis Niloticus L.), İsmi̇han Karayücel, Sedat Karayücel

Turkish Journal of Veterinary & Animal Sciences

The optimum UV duration times of eggs were examined in order to develop a simple and safe method for inducing androgenetic development in the Nile tilapia, Oreochromis niloticus. The yield of androgenetic haploid O. niloticus to the pigmentation stage was 18.53 ± 5.3% (relative to controls) with an optimal UV irradiation dose of 540 Jm^{-2} (at 150 µWcm^{-2 }) for 6 min. Most embryos developing after fertilisation with normal spermatozoa showed abnormal morphology and a haploid number of chromosomes (n = 22). The success of oocyte denucleation was also assessed by using the recessive "blond" skin pigmentation character.


The Effect Of Genetic Background On The In Vitro Development Of Mouse Embryos In Potassium Simplex Optimized Medium Supplemented With Amino Acids (Ksomaa), Haydar Bağiş, Hande Odaman Mercan, Hakan Sağirkaya, Gazi̇ Turgut, Andras Dinnyes Jan 2003

The Effect Of Genetic Background On The In Vitro Development Of Mouse Embryos In Potassium Simplex Optimized Medium Supplemented With Amino Acids (Ksomaa), Haydar Bağiş, Hande Odaman Mercan, Hakan Sağirkaya, Gazi̇ Turgut, Andras Dinnyes

Turkish Journal of Veterinary & Animal Sciences

In vitro culture systems are required for many biotechnological and assisted reproductive technologies. In order to perfect the conditions, the effect of genetic background was evaluated in the in vitro development of embryos from CD-1 and CB6F1 mice in potassium simplex optimized medium supplemented with amino acids (KSOMAA). Presumptive zygotes were recovered from superovulated and mated females (19-20 h post-hCG injection). Two-cell stage block, morula (M), expanded blastocyst (EB), and completely hatched blastocyst (CHB) rates and total cell count were compared in the two mouse strains. The development of CD-1 vs. CB6F1 embryos to 2-cell (98% vs. 96%) and to …


Comparison Of The Tocolytic Effects Of Ritodrine And Ca^{++} Channel Blockers On Serum Oestradiol And Progesterone Levels, Füsun Tosun, Aymelek Gönenç, Bolkan Şi̇mşek Jan 2003

Comparison Of The Tocolytic Effects Of Ritodrine And Ca^{++} Channel Blockers On Serum Oestradiol And Progesterone Levels, Füsun Tosun, Aymelek Gönenç, Bolkan Şi̇mşek

Turkish Journal of Veterinary & Animal Sciences

Experimental pregnancy was induced with gonadal steroids in in vivo ovariectomized rats. Conditions for premature delivery were established by giving a salt-rich diet and administering oxytocin to induce high-risk pregnancy. In order to avoid early delivery, a betamimetic ritodrine and the Ca^{++} channel blockers nitrendipine and nicardipine were administered to three separate groups. The effects of these three tocolytic agents were examined by determining serum oestradiol and progesterone levels. Additionally, systolic blood pressure was measured and tachycardia was observed. After the administration of tocolytic agents, it was observed that serum oestradiol levels were increased while there was a decrease in …


The Effects Of Formic Acid Or Formic Acid Plus Molasses Additives On The Fermentation Quality And Dm And Adf Degradabilities Of Grass Silage, Erol Baytok, Habi̇p Muruz Jan 2003

The Effects Of Formic Acid Or Formic Acid Plus Molasses Additives On The Fermentation Quality And Dm And Adf Degradabilities Of Grass Silage, Erol Baytok, Habi̇p Muruz

Turkish Journal of Veterinary & Animal Sciences

This experiment was carried out to evaluate the effects of formic acid and formic acid plus molasses additives on the grass silage fermentation characteristics and degradability of dry matter (DM) and acid-detergent fibre (ADF). Grasses were cut in early (experiment I), middle (experiment II) and late maturity (experiment III). The additive treatments in experiments I, II and III were: 1) Untreated; 2) 0.5% Formic acid (FA); 3) FA+2% molasses; 4) FA+4% molasses; and 5) FA+6% molasses. The silages were ensiled in jar silos (1 L) and DM and ADF degradability were determined by using the nylon bag technique in the …