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Method For Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Jose Cibelli
Method For Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Jose Cibelli
Jose Cibelli
Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish research community. In this chapter, we meticulously outline each step of the published protocol as well as preparations of equipments and reagents used in zebrafish SCNT.
Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Patrick J. Venta, C C. Chang, Jose B. Cibelli
Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Patrick J. Venta, C C. Chang, Jose B. Cibelli
Jose Cibelli
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.