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Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki

The Thai Journal of Veterinary Medicine

We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …