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Full-Text Articles in Medicine and Health Sciences
Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki
Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki
The Thai Journal of Veterinary Medicine
We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …
Identification Of Forensically Important Blowflies In Thailand Based On The Second Internal Transcribed Spacer Region Of Ribosomal Dna And The Nadh Dehydrogenase Subunit 5 Gene, R Jantorn, P Siriyasatien, A Tawatsin, U Thavara, K. Preativatanyou
Identification Of Forensically Important Blowflies In Thailand Based On The Second Internal Transcribed Spacer Region Of Ribosomal Dna And The Nadh Dehydrogenase Subunit 5 Gene, R Jantorn, P Siriyasatien, A Tawatsin, U Thavara, K. Preativatanyou
Chulalongkorn Medical Journal
Problem/Background: Estimation of postmortem interval (PMI) is essential for supporting justice especially in legal cases. At early stage of death, the PMI can be evaluated using data from physical changes of the corpses. In case of the corpses that have died more than 48 hours, the physical change may be less accurate for prediction. The species identification of blowflies, early found on corpses, as well as the specific developmental stage are required for PMI estimation. However, it is not yet practical for morphological identification. To surpass these problems, DNAbased identification is preferentially applied.