Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Medicine and Health Sciences
Retinal Pigment Epithelial Glycosaminoglycan Metabolism: Intracellular Versus Extracellular Pathways. In Vitro Studies In Normal And Diseased Cells, Gustavo D. Aguirre, Lawrence E. Stramm, M Haskins
Retinal Pigment Epithelial Glycosaminoglycan Metabolism: Intracellular Versus Extracellular Pathways. In Vitro Studies In Normal And Diseased Cells, Gustavo D. Aguirre, Lawrence E. Stramm, M Haskins
Gustavo D. Aguirre, VMD, PhD
The synthesis and turnover of glycosaminoglycans (GAGs) in different fractions of cultured feline retinal pigment epithelium (RPE) were characterized. In one method of fractionation, trypsin was used to separate the extracellular components (referred to as trypsin-soluble glycocalyx) from the intracellular components. As a second method, the basal extracellular matrix (basal ECM) was separated from the rest of the GAGs (cell-associated GAGs) by extracting the cell layer with NH4OH. The incorporation of 35SO4 into cetylpyridinium chloride-precipitable GAGs in the cell-associated and the intracellular fractions increased throughout the labeling period, while in the trypsin-soluble glycocalyx and the basal ECM incorporation approached a …