Medicine and Health Sciences Commons^™

A Preliminary Evaluation Of Calcitonin And Pdn-21 As Tumor Markers For Lung Cancer, J. J. Body, J. C. Dumon, J. P. Sculier, G. Dabouis, H. Lacroix, P. Libert, M. Richez, G. Bureau, P. Mommen, N. Raymakers, M. Paesmans, J. Klastersky

Henry Ford Hospital Medical Journal

Immunoreactive calcitonin (ICT) can be ectopically secreted by lung cancer cells and has been proposed as a tumor marker for bronchial neoplasms. Since PDN-21 (katacalcin or the carboxyl-terminal flanking peptide of the calcitonin gene) and CT are cosecreted in normal subjects and in patients with medullary thyroid carcinoma (MTC), we sought to determine the potential utility of PDN-21 as a tumor marker for lung cancer. We measured carcinoembryonic antigen (CEA), neuronspecific enolase (NSE), iCT, and PDN-21 in 119 to 378 healthy subjects, 88 to 91 patients with benign pulmonary diseases, and 249 patients with advanced lung cancer (108 small cell …

Evolutionary Pathways Of The Calcitonin (Calc) Genes, Cornelis J. M. Lips, Rolf A. Geerdink, Maria G. Nieuwenhuis, Jaap Van Der Sluys Veer

Henry Ford Hospital Medical Journal

Recombinant DNA techniques have made it possible to establish the structure of various genes encoding polypeptide hormones. Comparison of nucleotide sequences of the calcitonin (CALC) genes in man has revealed surprising similarities and variations. These findings and the homologies among the sequences in different species offered an opportunity for speculation about relationships between these genes and about their evolutionary origin. The first gene (CALC-I) directing the synthesis of calcitonin (CT) or CT gene-related peptide (CGRP) comprises six exons and gives rise to two mRNAs by an alternative RNA-processing mechanism. The homology between CGRP and CT reflects their common origin. The …

A Novel Parathyroid Hormone-Related Gene Product, T. J. Martin, J. A. Danks

Henry Ford Hospital Medical Journal

A parathyroid hormone-related protein (PTHrP) has been invoked as being responsible for the humoral hypercalcemia of malignancy. Eight of the first 13 amino acids of PTHrP are identical with those in PTH, but there is no other significant homology. The PTHrP gene is located on chromosome 12, whereas that for PTH is on chromosome 11, and the two genes are probably related by a duplication process. Antisera against PTHrP(1-34), which cross-read poorly or not at all with PTH, and antisera against other parts of PTHrP not homologous to PTH were used in immunocytochemistry, using a peroxidase-antiperoxidase method, to identify PTHrP …

Transcriptional Regulation Of The Human Calcitonin Gene: A Progress Report, Sara Peleg, Gilbert J. Cote, Ronald V. Abruzzese, Robert F. Gagel

Henry Ford Hospital Medical Journal

We have applied DNA transfer techniques lo study the transcriptional regulation of the calcitonin (CT) gene in a C-cell line (TT) derived from a human medullary thyroid carcinoma. TT cells were transfected with a fusion gene containing the CT gene promoter and 5' -flanking DNA attached to the promoter-less growth hormone gene (reporter). We quantitated the reporter gene product to monitor transcriptional activation by the CT promoter and deletion mutants of the 5' -flanking DNA. We found that the proximal CT promoter which includes the DNA sequence from +1 to -129 bp upstream from the CT transcription start site did …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Ua68/10/1 Dare In Nashville Schools 1988-89, John Faine

WKU Archives Records

This report summarizes the results of a partial evaluation of the 1988-89 Drug Abuse Resistance Education (DARE) effort implemented in all sixth grades in the Nashville, Tennessee, Metropolitan Public School system by trained instructors from the Nashville Metropolitan Police Department. As documented in the following pages, the evaluation effort had two major components.

1. An evaluation of the program by teachers and principals in all the participating schools.

2. Inner-City Study, an investigation designed to assess the impact of the program in elementary and middle schools that service those students thought to be most at risk to the threat of …

Closing In On The Men2a Locus, Nancy E. Simpson, Kenneth K. Kidd

Henry Ford Hospital Medical Journal

The mapping of the locus for multiple endocrine neoplasia type 2A (MEN 2A) to chromosome 10 using linkage is briefly reviewed including a discussion of linkage strategy and reference to some of the exclusions before the assignment. The subsequent development of the map of the centromeric region of the chromosome and the linking of what appear to be the four closest flanking markers and the centromeric alphoid sequence to the disease locus are reviewed. To date no recombination has been observed between the centromeric marker and the MEN2A locus among, at least, 26 informative meioses, 11 of which are phase …

Screening For Medullary Thyroid Cancer In France: A National Effort, Claude Calmettes, A. Chaventre, Nicole Feingold, Brigitte Franc, J. M. Guliana

Henry Ford Hospital Medical Journal

Screening for medullary thyroid cancer (MTC) in France is based on a protocol that has been widely distributed nationally. A network of coordinators utilizing a common questionnaire provides for an effective national screening program. Calcitonin stimulation procedures are systematically used for all first-degree relatives of MTC patients. Pathological studies utilize special immunopathologic techniques. Genealogic information is obtained on all index cases, and blood specimens are collected for establishing permanent cell lines. The data collected are used not only to establish the diagnosis of the hereditary or sporadic form of the disease but also to expand the screening as appropriate. This …

Screening For Pheochromocytoma In The Men 2 Syndrome, J. Kotzerke, C. Stibane, H. Dralle, H. Wiese, W. Burchert

Henry Ford Hospital Medical Journal

Twenty-two patients with the multiple endocrine neoplasia type 2 (MEN 2) syndrome were screened for pheochromocytoma since it is a major cause of morbidity in MEN 2 families. Clinical symptoms, biochemical parameters, ultrasound, computed tomography or magnetic resonance imaging, and meta-iodo-benzylguanidine (MIBG) scintigraphy were evaluated for detection of adrenal tumors. Clinical symptoms and plasma or urine catecholamines appeared to be nonspecific, whereas MIBG scintigraphy was highly specific and the most sensitive parameter. Patients older than age 30 should be scintigraphically screened at least once despite the radiation exposure. Demonstration of only slight uptake is not an indication for surgery but …

Postoperative Pentagastrin-Stimulated Serum Calcitonin Concentrations In Patients With Medullary Thyroid Carcinoma: Reoperations In Patients With Concentrations Bordering The Detection Limit, Hinrich Meybier, Heinrich Schmidt-Gayk, Heinz Buhr, Friedhelm Raue

Henry Ford Hospital Medical Journal

The case reports on two patients with medullary thyroid carcinoma show that even postoperatively stimulated serum calcitonin (CT) concentrations near the detection limit (using a polyclonal antibody against synthetic CT) can demonstrate persistent disease. Stimulated CT concentrations can be lowered to nondetectable levels by a second and third operation if a meticulous technique is used for dissection of the lymph compartments. The patient can then be assumed lo be cured. Diagnostic accuracy at very low CT concentrations can be improved by selective venous catheterization with blood sampling for CT after stimulation.

Familial Cutaneous Lichen Amyloidosis In Association With Multiple Endocrine Neoplasia Type 2a: A New Variant, D. T. Donovan, M. L. Levy, E. J. Furst, B. R. Alford, T. Wheeler, J. A. Tschen, R. F. Gagel

Henry Ford Hospital Medical Journal

Multiple endocrine neoplasia type 2A (MEN 2A) is a rare hereditary disease transmitted in families as an autosomal dominant trad. We have identified a family in which the expression of a rare autosomal dominant form of cutaneous lichen amyloidosis appears to cosegregate with MEN 2A. In this family the skin lesion presented as multiple infiltrated papules overlying well demarcated plaques over the scapular area (right or left). Immunohistochemical studies demonstrated amyloid which stained for keratin but not calcitonin. A total of 19 members were screened. Three members of the family have the characteristic skin lesion and MEN 2A; two additional …

Use Of Somatostatin Analog Sms 201-995 In Medullary Thyroid Carcinoma, Alfonso Libroia, Uberta Verga, Gianleone Di Sacco, Marco Piolini, Fabrizio Muratori

Henry Ford Hospital Medical Journal

We have studied seven subjects with medullary thyroid carcinoma. Each had elevated basal serum calcitonin (CT) levels following total thyroidectomy. After subcutaneous administration of 100 μg of SMS 201-995, blood samples were collected at 60-minute intervals for six hours. Two patients showed a marked decrease of CT levels (patient A: baseline 565 μg/mL, nadir 150 μg/mL; patient B: baseline 1,632 μg/mL, nadir 416 μg/mL). The other five patients showed no significant change in comparison with saline infusion. Two patients were treated with SMS 201-995 (300 μg/day)for 90 days. One of these patients responded to the acute SMS 201-995 test and …

Localization Of Metastases From Medullary Thyroid Carcinoma Using Different Methods, Rosa C. Cabezas, Luis Berna, Montserrat Estorch, Ignacio Carrio, Angeles Garcia-Ameijeiras

Henry Ford Hospital Medical Journal

We analyzed the efficiency of three different noninvasive methods in the localization of recurrent medullary thyroid carcinoma (MTC). Nine patients (six females and three males) with biochemical evidence of disease after primary surgery were subjected to anti-carcinoembryonic antigen (anti-CEA) antibody, meta-iodo-benzylguanidine (MIBG), and computed tomography. Another female patient, in biochemical remission for six years after initial surgery, was also studied using the same methods. Three of the ten patients had negative results with all three methods (including the patient in remission). The other seven patients showed abnormal uptake of labeled anti-CEA antibody in various localizations: only two of these patients …

Dna Cytophotometric Findings In Pheochromocytoma, Barbara-Christina Padberg, Esther Garbe, Eike Achilles, Henning Dralle, Max Bressel, Soren Schroeder

Henry Ford Hospital Medical Journal

Fifty adrenalectomy specimens containing normal (n = 3), hyperplastic (n =4), or neoplastic (n = 43) medullary tissue were subjected lo quantitative measurements of DNA content. Of the 43 pheochromocytomas, 16 were neoplasms inherited in the setting of multiple endocrine neoplasia type 2A. Five of 27 sporadic pheochromocytomas followed a malignant clinical course. Follow-up data were available in 25 patients. In normal medulla and adrenomedullary hyperplasia, either diploid or euploid DNA distributions were found. In contrast, 87% (33 of 38) of the benign and all five malignant pheochromocytomas exhibited nondiploid or aneuploid DNA histograms. No differences in DNA content existed …

Modulation Of Calcitonin Secretion By Modification Of Calcium Channels?, Hans Scherubl, Friedhelm Raue, Michael Hoflich, Reinhard Ziegler

Henry Ford Hospital Medical Journal

Voltage-dependent calcium channels (VDCC) regulating Ca++ influx through the cellular plasma membrane play a major role in the Ca++ -induced calcitonin (CT) secretion. Using rat C-cells (rMTC 6-23 cell line), we have studied the effect of repetitive stimulation by either Ca++ (2 mM) or glucagon (10 μM) or epinephrine (10 μM) on CT secretion. Following a Ca++ -induced initial rise, CT release declined to basal levels after about four hours despite high Ca++ ; addition of 10 μM glucagon to the "Ca++ desensitized C-cells" yielded the normal stimulatory effect of glucagon on CT release. Repetitive stimulation with glucagon showed a …

Abstracts From The Third International Workshop On Men 2

Henry Ford Hospital Medical Journal

No abstract provided.

Commencement Program 1989 (Summer Conferring Of Degrees), Loma Linda University

Commencement Programs

CONTENTS

3 | The Academic Procession

4 | Conferring of Degrees

6 | Candidates for Degrees

16 | Awards

An Analysis Of Selected Family Life Education Curricula, Phoebe Terry Butler

Community & Environmental Health Theses & Dissertations

The purpose of this study was to examine the content of family life education curricula in Virginia's public schools and determine its appropriateness for the mentally handicapped students. It was the intent of this study to determine if existing curricula were designed to address the needs of mentally handicapped students according to a standard curricula designed by the American Association for Health, Physical Education and Recreation and the Sex Information and Education Council of the United States. The 15 participants of this study were selected from a random sample of 69. The response rate was 20.2%.

A letter soliciting family …

Aids Prevention Through Printed Media: Knowledge And Communication Behaviors Of Gay College-Aged Males, Cheryl L. Knight

Masters Theses

AIDS prevention research has established the effectiveness of multiple component group interventions. It has not identified whether the effectiveness is due to the format of the intervention (i.e., rehearsal, feedback, etc.) or its instructional content. This study investigates the impact of instructional content delivered in the inexpensive and readily obtained printed medium. Thirteen gay or bisexual college-aged men received an information pamphlet and an AIDS-related communication skills training booklet. Subjects were evaluated on measures of knowledge, safe and risky behaviors, and communication behaviors in each of three assessment sessions. Visual analyses and repeated measures analyses of variance did not support …

Women, Water, And Sanitation: Household Water Use In Two Egyptian Villages, Samiha El Katsha, Awatif Younis, Olfat El Sebaie, Ahmed Hussein

Faculty Books

No abstract provided.

Women And Crack Addiction, Mary Guinan

Public Health Faculty Publications

A smokable form of cocaine has recently been introduced to the United States. First documented in New York in 1985, the use of rock cocaine or crack has spread rapidly throughout the country since then. Until crack appeared, noninjectable cocaine use was largely a problem in the middle and upper classes because of its high price. Crack has changed that; five and ten dollar packages are readily available. Crack produces a short-lived high and may enhance sexual desire and performance. The rapidity of the spread of crack in inner cities is extraordinary but the movement into rural areas has been …

The Aids Project Newsletter, Vol.2, No.5 (July 1989), David Ketchum, The Aids Project

AIDS Project Newsletter (1987-1991)

No abstract provided.

Barren River District Health Department Health Education/Risk Reduction Demonstration Projects, Rebecca Bruce

Masters Theses & Specialist Projects

In July 1980, the Barren River District Health Department (BRDHD), serving eight counties (combined population approximately 204,000) in Southcentral Kentucky, was selected as a demonstration site under the auspices of the federal Health Education Risk Reduction (HERR) Program. With continued HERR funding for eight years, the BRDHD developed several successful health promotion projects. Major components of these projects include: 1) community health promotion, which serves to identify high -risk groups in the community and provide them with health education-health promotion services, 2) school health education which included the development of a preschool health education curriculum, 3) teacher education workshop, which …

Identification And Ranking Of Stressors In Nuclear Medicine Technology, Scott R. Sechrist

Community & Environmental Health Theses & Dissertations

The purpose of this study was to identify the most significant stressors in the field of nuclear medicine technology. A sample of convenience of 25 nuclear medicine technologists was used to generate a list of 35 stressors specific to the occupation. Sixty-three randomly selected nuclear medicine technologists responded to a mailed questionnaire which asked the technologist to assess the relative stressfulness of each of the 35 items on a scale of "0 to 1000". Data from the 59 valid responses indicated that equipment malfunctions, add on exams, uncooperative physicians, lack of staff, and uncooperative patients were the most significant stressors …

Religious Commitment As A Predictor Of Health Behavior And Health Status In A Selected Population, Donald George King

Loma Linda University Electronic Theses, Dissertations & Projects

Religious beliefs and behaviors are multidimensional and they profoundly determine the ways we respond to health and illness, suffering and death. Researchers have designed numerous scales to measure different dimensions of religious commitment, beliefs and behaviors. A thorough review of the literature has shown general relationships between religion and health, but no researcher has developed an instrument measuring dimensions of religious commitment specifically relevant to health behaviors.

In this study, scale items were developed to measure three dimensions of religious beliefs thought by Kenneth Vaux (1976) to be related to health behavior--purity of life, peace in existence, and belief in …

Hypoglycemia After Administration Of Somatostatin Analog (Sms 201-995) In Metastatic Carcinoid, John E. Brunner, Davida F. Kruger, Michael A. Basha, Eyal Meiri, Scott S. Kaatz

Henry Ford Hospital Medical Journal

SMS 201-995 (Sandoz Pharmaceuticals. East Hanover NJ) is a synthetic peptide analog of native somatostatin that has been used to relieve .symptoms caused by neuroendocrine tumors. Reports have described an insulin suppressive effect of SMS 201-995 that results in elevations of blood glucose. We report a patient with a metastatic small bowel carcinoid and renal failure in whom mild symptomatic hypoglycemia occurred 30 to 60 minutes after SMS 201-995 administration. No increase in insulin or decreases in glucagon. Cortisol, or catecholamines were observed during these hypoglycemic episodes. Elevated levels of growth hormone fell gradually following SMS 201-995 administration and did …

A Suggested New Approach To Supraventricular Tachydysrhythmia After Coronary Bypass Surgery, Walter Kao, Mihai Gheorghiade

Henry Ford Hospital Medical Journal

No abstract provided.

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Recurrent Leukemia Cutis In Acute Myeloblastic Leukemia, Reda A. Girgis, Howard Terebelo, Koichi Maeda

Henry Ford Hospital Medical Journal

We report the case of a 64-year-old female with acute myeloblastic leukemia (French-American- British classification: M2) who developed two specific cutaneous manifestations during her illness. She presented with extensive cellulitis involving the face, neck, and upper chest wall. While the cellulitis resolved with antibiotic therapy, a fungating ulcerated nodule remained on the lower lip which proved to be leukemic on biopsy. Concomitant blood and bone marrow findings were diagnostic of acute myeloblastic leukemia. The lip lesion cleared with a course of chemotherapy. An erythematous macular rash subsequently developed over the lower trunk which was thought to be an allergic reaction …

Toxic Shock-Like Syndrome Associated With Necrotizing Streptococcus Pyogenes Infection, Thomas J. Connolly, Donald J. Pavelka, Eugene F. Lanspa, Thomas L. Connolly

Henry Ford Hospital Medical Journal

Two patients with toxic shock-like syndrome are presented. Both patients had necrotizing cellulitis due to Streptococcus pyogenes, and both patients required extensive surgical debridement. The association of Streptococcus pyogenes infection and toxic shock-like syndrome is discussed.