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Full-Text Articles in Medicine and Health Sciences
Stable Isotope-Resolved Metabolomics Shows Metabolic Resistance To Anti-Cancer Selenite In 3d Spheroids Versus 2d Cell Cultures, Teresa W. -M. Fan, Salim S. El-Amouri, Jessica K. A. Macedo, Qing Jun Wang, Huan Song, Teresa Cassel, Andrew N. Lane
Stable Isotope-Resolved Metabolomics Shows Metabolic Resistance To Anti-Cancer Selenite In 3d Spheroids Versus 2d Cell Cultures, Teresa W. -M. Fan, Salim S. El-Amouri, Jessica K. A. Macedo, Qing Jun Wang, Huan Song, Teresa Cassel, Andrew N. Lane
Center for Environmental and Systems Biochemistry Faculty Publications
Conventional two-dimensional (2D) cell cultures are grown on rigid plastic substrates with unrealistic concentration gradients of O2, nutrients, and treatment agents. More importantly, 2D cultures lack cell–cell and cell–extracellular matrix (ECM) interactions, which are critical for regulating cell behavior and functions. There are several three-dimensional (3D) cell culture systems such as Matrigel, hydrogels, micropatterned plates, and hanging drop that overcome these drawbacks but they suffer from technical challenges including long spheroid formation times, difficult handling for high throughput assays, and/or matrix contamination for metabolic studies. Magnetic 3D bioprinting (M3DB) can circumvent these issues by utilizing nanoparticles that enable …
Noninvasive Liquid Diet Delivery Of Stable Isotopes Into Mouse Models For Deep Metabolic Network Tracing, Ramon C. Sun, Teresa W.-M. Fan, Pan Deng, Richard M. Higashi, Andrew N. Lane, Anh-Thu Le, Timothy L. Scott, Qiushi Sun, Marc O. Warmoes, Ye Yang
Noninvasive Liquid Diet Delivery Of Stable Isotopes Into Mouse Models For Deep Metabolic Network Tracing, Ramon C. Sun, Teresa W.-M. Fan, Pan Deng, Richard M. Higashi, Andrew N. Lane, Anh-Thu Le, Timothy L. Scott, Qiushi Sun, Marc O. Warmoes, Ye Yang
Center for Environmental and Systems Biochemistry Faculty Publications
Delivering isotopic tracers for metabolic studies in rodents without overt stress is challenging. Current methods achieve low label enrichment in proteins and lipids. Here, we report noninvasive introduction of 13C6-glucose via a stress-free, ad libitum liquid diet. Using NMR and ion chromatography-mass spectrometry, we quantify extensive 13C enrichment in products of glycolysis, the Krebs cycle, the pentose phosphate pathway, nucleobases, UDP-sugars, glycogen, lipids, and proteins in mouse tissues during 12 to 48 h of 13C6-glucose feeding. Applying this approach to patient-derived lung tumor xenografts (PDTX), we show that the liver supplies glucose-derived Gln …