Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 16 of 16
Full-Text Articles in Medicine and Health Sciences
Comparison Of Hyperbaric Oxygen And Medical Ozone Therapies In A Rat Model Of Experimental Distal Colitis, O. Altinel, S. Demirbas, E. Cakir, H. Yaman, I. Ozerhan, Eyup Duran, T. Cayci, E. Akgul, N. Ersoz, B. Uysal, B. Kurt, M. Yasar, S. Oter, Y. Peker
Comparison Of Hyperbaric Oxygen And Medical Ozone Therapies In A Rat Model Of Experimental Distal Colitis, O. Altinel, S. Demirbas, E. Cakir, H. Yaman, I. Ozerhan, Eyup Duran, T. Cayci, E. Akgul, N. Ersoz, B. Uysal, B. Kurt, M. Yasar, S. Oter, Y. Peker
Eyup Hakan Duran
No abstract provided.
Lipiodol Versus Methylene Blue For Prevention Of Postsurgical Adhesion Formation In A Rat Model., E. Duran, Esra Kuscu, Hulusi Zeyneloglu, Esra Saygili, Sertac Batioglu
Lipiodol Versus Methylene Blue For Prevention Of Postsurgical Adhesion Formation In A Rat Model., E. Duran, Esra Kuscu, Hulusi Zeyneloglu, Esra Saygili, Sertac Batioglu
Eyup Hakan Duran
OBJECTIVE: To determine the effects of intraperitoneal Lipiodol and methylene blue in prevention of postsurgical adhesion formation. STUDY DESIGN: Thirty female rats were divided into three equal groups and a standard damage to provoke adhesion formation was made by laparotomy to each of them. Methylene blue and Lipiodol was administered intraperitoneally to the first and second groups, respectively; while the third group was taken as control and no additional intervention was made. Adhesions were scored by a second look laparotomy which was made 21 days after the first operation. RESULTS: Adhesion scores of the second group (i.e. Lipiodol group) were …
Expression And Localization Of Inducible And Endothelial Nitric Oxide Synthase In The Rat Ovary. Effects Of Gonadotropin Stimulation In Vivo, Bradley Van Voorhis, K. Moore, P. Strijbos, S. Nelson, S. Baylis, D. Grzybicki, C. Weiner
Expression And Localization Of Inducible And Endothelial Nitric Oxide Synthase In The Rat Ovary. Effects Of Gonadotropin Stimulation In Vivo, Bradley Van Voorhis, K. Moore, P. Strijbos, S. Nelson, S. Baylis, D. Grzybicki, C. Weiner
Bradley J Van Voorhis
Nitric oxide is reportedly involved in the regulation of several ovarian processes, yet the isoforms of nitric oxide synthase (NOS) expressed in the ovary are unknown. Our purpose was to identify and localize NOS isoenzymes in the rat ovary and to examine++ if mRNA expression of NOS isoenzymes change after gonadotropin stimulation. Using reverse transcriptase-PCR, we demonstrated that inducible (iNOS) and endothelial (eNOS), but not neuronal, NOS mRNAs are expressed in the ovary. In a gonadotropin-stimulated rat model, unstimulated ovaries had the highest levels of iNOS mRNA as quantified by ribonuclease protection assay. After gonadotropin injection, iNOS mRNA declined to …
Comparison Of In Vivo Bioavailability Of Progestational Agents Into The Rat Uterus And Liver And The Effect Of Plasma Protein Binding, Bradley Van Voorhis, D. Matt, M. Tedeschi, K. Steingold
Comparison Of In Vivo Bioavailability Of Progestational Agents Into The Rat Uterus And Liver And The Effect Of Plasma Protein Binding, Bradley Van Voorhis, D. Matt, M. Tedeschi, K. Steingold
Bradley J Van Voorhis
The in vivo bioavailabilities of three clinically available progestational agents were evaluated with a previously described rat model. With this model a single-injection, double-isotope technique was performed to calculate and compare the unidirectional extractions of progesterone, 19-nortestosterone, and medroxyprogesterone acetate into the rat uterus and liver. To assess the role of plasma protein binding in the extraction of these agents, steroids were presented in four different vehicles: (1) Ringer's solution, 0.1% bovine serum albumin; (2) Ringer's solution, 4% bovine serum albumin; (3) Ringer's solution, 0.8 mg/ml, alpha 1-acid glycoprotein; (4) human pregnancy serum. The results revealed that liver extraction always …
Effect Of Hydrogen Peroxide On Prostaglandin Production And Contractions Of The Pregnant Rat Uterus, P. Cherouny, R. Ghodgaonkar, Jennifer Niebyl, N. Dubin
Effect Of Hydrogen Peroxide On Prostaglandin Production And Contractions Of The Pregnant Rat Uterus, P. Cherouny, R. Ghodgaonkar, Jennifer Niebyl, N. Dubin
Jennifer R Niebyl
Although evidence for a role for prostaglandins in parturition is abundant, less is known about how prostaglandin levels are regulated at term. Conditions occurring peripartum in the uteroplacental unit can result in reactive oxygen production. We investigated the effect of one reactive oxygen product, hydrogen peroxide, on in vitro activity of uterine segments from the 18-day-pregnant rat. H2O2 (0.3 mmol/L) was found to elicit rhythmic contractions and increase prostaglandins F2 alpha and E2 release by uterine tissue. Indomethacin blocked both of these effects. We conclude that H2O2 stimulates uterine contractions through a prostaglandin release mechanism. A speculative hypothesis of peripartum …
Preterm Parturition. Prostaglandin Synthetase Inhibitors, Jennifer Niebyl
Preterm Parturition. Prostaglandin Synthetase Inhibitors, Jennifer Niebyl
Jennifer R Niebyl
No abstract provided.
Encapsulated Beta-Islet Cells As A Bioartificial Pancreas To Treat Insulin-Dependent Diabetes During Pregnancy, Stephen Hunter, Y. Wang, C. Weiner, Jennifer Niebyl
Encapsulated Beta-Islet Cells As A Bioartificial Pancreas To Treat Insulin-Dependent Diabetes During Pregnancy, Stephen Hunter, Y. Wang, C. Weiner, Jennifer Niebyl
Jennifer R Niebyl
OBJECTIVE: Our purpose was to determine the effectiveness of the bioartificial pancreas technique in correcting (1) maternal carbohydrate metabolism and (2) fetal malformation rates in a pregnant diabetic animal model. STUDY DESIGN: Insulin secretion from encapsulated rat islets cultured in the presence of homologous rat prolactin was determined and compared with that of controls. Streptozotocin-induced diabetic Balb/c mice were then transplanted with rat islet cells encapsulated within alginate microbeads and were then bred. Blood glucose determinations were made after transplantation and throughout gestation. Pups were delivered by cesarean section on day 19 of gestation. Outcome parameters from the transplanted study …
In Vivo Biocompatibility Evaluation Of Cibacron Blue-Agarose, J. Kao, R. Rose, M. Yousef, Stephen Hunter, V. Rodgers
In Vivo Biocompatibility Evaluation Of Cibacron Blue-Agarose, J. Kao, R. Rose, M. Yousef, Stephen Hunter, V. Rodgers
Stephen K. Hunter
This study investigated the biocompatibility of Cibacron blue-agarose as a biomaterial for microencapsulation. Cibacron blue-agarose is known to have an affinity for albumin under certain pH conditions and in the proper steric environment. Thus it was postulated that the material's high affinity for host albumin might reduce a secondary immune response and reduce the fibrotic overgrowth that often accompanies transplanted foreign materials. In vivo tests were performed using the Lewis rat model. Both Cibacron blue-agarose and plain agarose disks were prepared, with some disks from each group being pre-exposed to sera from Lewis rats. The disks were transplanted into the …
Promotion Of Neovascularization Around Hollow Fiber Bioartificial Organs Using Biologically Active Substances, Stephen Hunter, J. Kao, Y. Wang, J. Benda, V. Rodgers
Promotion Of Neovascularization Around Hollow Fiber Bioartificial Organs Using Biologically Active Substances, Stephen Hunter, J. Kao, Y. Wang, J. Benda, V. Rodgers
Stephen K. Hunter
A limiting factor of the long-term function of bioartificial organs is oxygen delivery to the encapsulated tissue. This study determined whether incorporation of endothelial cell growth factor (ECGF) into the alginate core of a hollow fiber bioartificial organ will induce neovascularization around the hollow fiber. Polyethersulfone (PES) and polyvinylidine difluoride (PVDF) hollow fibers were examined. Endothelial cell growth factor was incorporated into sodium alginate, extruded into the lumen of hollow fibers, and cured in calcium chloride. Samples without ECGF were fabricated and used as controls. Hollow fibers were implanted into 16 rats. For each rat, two implants were placed subcutaneously …
Encapsulated Beta-Islet Cells As A Bioartificial Pancreas To Treat Insulin-Dependent Diabetes During Pregnancy, Stephen Hunter, Y. Wang, C. Weiner, Jennifer Niebyl
Encapsulated Beta-Islet Cells As A Bioartificial Pancreas To Treat Insulin-Dependent Diabetes During Pregnancy, Stephen Hunter, Y. Wang, C. Weiner, Jennifer Niebyl
Stephen K. Hunter
OBJECTIVE: Our purpose was to determine the effectiveness of the bioartificial pancreas technique in correcting (1) maternal carbohydrate metabolism and (2) fetal malformation rates in a pregnant diabetic animal model. STUDY DESIGN: Insulin secretion from encapsulated rat islets cultured in the presence of homologous rat prolactin was determined and compared with that of controls. Streptozotocin-induced diabetic Balb/c mice were then transplanted with rat islet cells encapsulated within alginate microbeads and were then bred. Blood glucose determinations were made after transplantation and throughout gestation. Pups were delivered by cesarean section on day 19 of gestation. Outcome parameters from the transplanted study …
Bioartificial Pancreas Use In Diabetic Pregnancy, Stephen Hunter, Y. Wang, V. Rodgers
Bioartificial Pancreas Use In Diabetic Pregnancy, Stephen Hunter, Y. Wang, V. Rodgers
Stephen K. Hunter
The purpose of this study was to evaluate the feasibility and effectiveness of Bioartificial Pancreas (BAP) technology use during diabetic pregnancy. In particular, the study asked 1) can microencapsulated islet cells effectively correct carbohydrate metabolism during diabetic pregnancy and 2) will such therapy, if initiated before conception, eliminate diabetes-induced congenital malformations in the fetus? Streptozotocin-induced diabetic female mice (ICR) received transplants of rat islets encapsulated within alginate microbeads. Animals were placed with male mice and bred. Random, nonfasting blood glucose (BG) determinations were made posttransplantation and throughout pregnancy. Pups were delivered by cesarean section on day 19 of gestation. Outcome …
Mathematical Modeling Of Myoglobin Facilitated Transport Of Oxygen In Devices Containing Myoglobin-Expressing Cells, H. Tilakaratne, Stephen Hunter, V. Rodgers
Mathematical Modeling Of Myoglobin Facilitated Transport Of Oxygen In Devices Containing Myoglobin-Expressing Cells, H. Tilakaratne, Stephen Hunter, V. Rodgers
Stephen K. Hunter
Low pO(2) is perhaps the most significant factor in artificial pancreas failure. In these environments, not only is the beta cell production of insulin reduced, but the cell death rate is also significantly higher. Mathematical models are developed to test the feasibility of facilitated oxygen transport in enhancing O(2) flux to genetically engineered cells in a bioartificial device such as a pancreas. For this device, it is proposed that beta cells be genetically engineered to express myoglobin throughout the cell. In addition, the significance of including myoglobin throughout the alginate matrix present to provide immuno-protection for the transplanted cells is …
The Nitric Oxide-Cyclic Gmp Pathway Plays An Essential Role In Both Promoting Cell Survival Of Cerebellar Granule Cells In Culture And Protecting The Cells Against Ethanol Neurotoxicity, N. Pantazis, J. West, Donghai Dai
The Nitric Oxide-Cyclic Gmp Pathway Plays An Essential Role In Both Promoting Cell Survival Of Cerebellar Granule Cells In Culture And Protecting The Cells Against Ethanol Neurotoxicity, N. Pantazis, J. West, Donghai Dai
Donghai Dai
NMDA has two beneficial effects on primary neuronal cultures of cerebellar granule cells (CGCs) established from 10-day-old rat pups. First, NMDA is neurotrophic and will enhance survival of CGCs in culture in the absence of ethanol. Second, ethanol exposure will induce cell death in CGC cultures, and NMDA can lessen this ethanol-induced cell loss, i.e., NMDA is neuroprotective. Because NMDA can stimulate production of nitric oxide (NO), which can in turn enhance synthesis of cyclic GMP, this study tested the hypothesis that the NO-cyclic GMP pathway is essential for NMDA-mediated neurotrophism and neuroprotection. Inhibiting the synthesis of NO with N(G)-nitro-L-arginine …
Transfection Of C6 Glioma Cells With Glia Maturation Factor Upregulates Brain-Derived Neurotrophic Factor And Nerve Growth Factor: Trophic Effects And Protection Against Ethanol Toxicity In Cerebellar Granule Cells, N. Pantazis, A. Zaheer, Donghai Dai, S. Zaheer, S. Green, R. Lim
Transfection Of C6 Glioma Cells With Glia Maturation Factor Upregulates Brain-Derived Neurotrophic Factor And Nerve Growth Factor: Trophic Effects And Protection Against Ethanol Toxicity In Cerebellar Granule Cells, N. Pantazis, A. Zaheer, Donghai Dai, S. Zaheer, S. Green, R. Lim
Donghai Dai
Glial cells play active roles in neuronal survival, as well as neuroprotection against toxic insult. Recent studies suggest that the brain protein glia maturation factor (GMF) is involved in intracellular signaling in glia. This study investigated whether or not GMF plays a role in the survival-promoting and neuroprotective functions of glia. C6 glioma cells were transfected in vitro with GMF utilizing an adenovirus vector. The transfected cells overexpressed GMF intracellularly, but did not secrete the protein. The conditioned medium (CM) was obtained from the GMF-transfected cells (CM-GMF) and tested on primary neuronal cultures, consisting of cerebellar granule cells (CGC). The …
Fgf-2, Ngf And Igf-1, But Not Bdnf, Utilize A Nitric Oxide Pathway To Signal Neurotrophic And Neuroprotective Effects Against Alcohol Toxicity In Cerebellar Granule Cell Cultures, D. Bonthius, B. Karacay, Donghai Dai, N. Pantazis
Fgf-2, Ngf And Igf-1, But Not Bdnf, Utilize A Nitric Oxide Pathway To Signal Neurotrophic And Neuroprotective Effects Against Alcohol Toxicity In Cerebellar Granule Cell Cultures, D. Bonthius, B. Karacay, Donghai Dai, N. Pantazis
Donghai Dai
Neuronal death is a prominent neuropathological component of fetal alcohol syndrome (FAS). Identification of molecular agents and pathways that can ameliorate alcohol-induced cell loss offers possible therapeutic strategies for FAS and potential insight into its pathogenesis. This study investigated the effects of growth factors on cellular survival in alcohol-exposed cerebellar granule cell (CGC) cultures and examined the role of the nitric oxide (NO)-cGMP-PKG (cGMP-dependent protein kinase) pathway in the cell survival-promoting effects of these growth factors. Primary CGC cultures were exposed to 0 or 400 mg/dl ethanol, accompanied by either no growth factor or 30 ng/ml fibroblast growth factor-2 (FGF-2), …
Relative Contribution Of Nedd4 And Nedd4-2 To Enac Regulation In Epithelia Determined By Rna Interference., Peter Snyder, Jennifer Steines, Diane Olson
Relative Contribution Of Nedd4 And Nedd4-2 To Enac Regulation In Epithelia Determined By Rna Interference., Peter Snyder, Jennifer Steines, Diane Olson
Jennifer C. Steines Wagemester
Epithelial Na+ transport is regulated in large part by mechanisms that control expression of the epithelial Na+ channel (ENaC) at the cell surface. Nedd4 and Nedd4-2 are candidates to control ENaC surface expression, but it is not known which of these proteins contributes to ENaC regulation in epithelia. To address this question, we used RNA interference to selectively reduce expression of Nedd4 or Nedd4-2. We found that endogenous Nedd4-2, but not Nedd4, negatively regulates ENaC in two epithelial cell lines (Fischer rat thyroid and H441); small interfering RNA (siRNA) against Nedd4-2 increased amiloride-sensitive Na+ current (compared with control siRNA), but …