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Full-Text Articles in Medicine and Health Sciences
Investigation Of Oxidative Stress Status In Cumulus Cells In Patıents With In Vitrofertilization, Rabi̇a Tural, Cengi̇z Karakaya, Mehmet Erdem, Zeynep Aykol, Recep Onur Karabacak, Mustafa Kavutçu
Investigation Of Oxidative Stress Status In Cumulus Cells In Patıents With In Vitrofertilization, Rabi̇a Tural, Cengi̇z Karakaya, Mehmet Erdem, Zeynep Aykol, Recep Onur Karabacak, Mustafa Kavutçu
Turkish Journal of Medical Sciences
Background/aim: The negative impact of oxidative stress on oocytes obtained from in vitro fertilization (IVF) patients is a challenge for the optimization of live birth rates. In this study, it is aimed to investigate whether oxidant/antioxidant parameters have a predictive value in terms of determining the count and quality of oocytes. Materials and methods: Catalase (CAT), glutathione-S-transferase (GST), arylesterase (ARE) enzyme activities, and malondialdehyde (MDA) levels were analysed in cumulus cells of poor responder (n = 28, oocyte count ≤ 4), normo responder (n = 48, 5 ≤ oocyte count ≤ 14), and high responder (n = 26, oocyte count …
Salivary Glucose And Antioxidant Defense Markers In Type Ii Diabetes Mellitus, Sayeeda Mussavira, Mala Dharmalingam, Bindhu Omana Sukumaran
Salivary Glucose And Antioxidant Defense Markers In Type Ii Diabetes Mellitus, Sayeeda Mussavira, Mala Dharmalingam, Bindhu Omana Sukumaran
Turkish Journal of Medical Sciences
To evaluate salivary antioxidant defense markers, their correlation with salivary glucose, and glycemic status in type II diabetes mellitus (DM). Materials and methods: The study included 53 diabetic patients and 40 healthy subjects. Salivary glucose, blood glucose, and uric acid (UA) were determined by specific enzymatic methods. Total antioxidant activity (AOA), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein were determined spectrophotometrically. Results: Salivary UA (3.12 vs. 1.89 mg/dL), GSH (47 vs. 11.92 µg/mL), and total protein (375.12 vs. 202.23 mg/dL) were significantly higher (P < 0.001; r = 0.455, 0.735, 0.498 respectively) and AOA (653.1 vs. 897.3 µmol/L) was significantly lower in the DM group (P < 0.001, r = -0.431) compared to healthy controls. Among the antioxidant enzymes, CAT was significantly lower (1214 vs. 9468.9 kat) in the DM group (P < 0.001, r = -0.886). Spearman correlation analyses within the diabetic group showed a strong positive association between salivary glucose and blood glucose (P < 0.001, r = 0.9), salivary glucose and GSH, and salivary glucose and UA. Salivary glucose showed a negative correlation with AOA and CAT (P = 0.008, r = -0.447) in the diabetic group. Conclusion: Findings of this study, showing a strong correlation between salivary glucose and blood glucose as well as changes in antioxidant components in the DM group, suggest that saliva can be used for the diagnosis and management of DM.
Malondialdehyde And Nitric Oxide Levels And Catalase, Superoxide Dismutase, And Glutathione Peroxidase Levels In Maternal Blood During Different Trimesters Ofpregnancy And In The Cord Blood Of Newborns, Sevda Yüksel, Ayşe Arzu Yi̇ği̇t
Malondialdehyde And Nitric Oxide Levels And Catalase, Superoxide Dismutase, And Glutathione Peroxidase Levels In Maternal Blood During Different Trimesters Ofpregnancy And In The Cord Blood Of Newborns, Sevda Yüksel, Ayşe Arzu Yi̇ği̇t
Turkish Journal of Medical Sciences
Background/aim: To determine whether levels of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) change during the 3 trimesters of pregnancy, and to compare the third trimester of pregnancy with newborn cord blood in respect to the levels of MDA, NO, and antioxidant enzymes. Materials and methods: Maternal blood samples were collected at 9?13, 22?26, and 36?40 weeks of gestation. Cord blood was collected at the time of delivery. Results: NO levels and GPx activity were higher in the second and third trimesters than in the first trimester. MDA level was lower in the …
A Comparison Of Erythrocyte Superoxide Dismutase And Catalase Activity In Patients With Hepatitis C Infection, Aysun Bay Karabulut, Emi̇ne Sönmez, Yaşar Bayindir, Engi̇n Gözükara
A Comparison Of Erythrocyte Superoxide Dismutase And Catalase Activity In Patients With Hepatitis C Infection, Aysun Bay Karabulut, Emi̇ne Sönmez, Yaşar Bayindir, Engi̇n Gözükara
Turkish Journal of Medical Sciences
Hepatitis C virus (HCV) infection remains important due to difficulties in treatment up to a chronic state. It is considered that free radicals, lipid peroxidation and antioxidant defense play a role in various tissue damages, just as in certain types of viral hepatitis. Since only limited data has been reported concerning oxidative stress in viral hepatitis, a comparative study was planned for patients with hepatitis C. In this study, we searched for erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities in patients with HCV infection who had received and not received recombinant interferon alpha. Four groups of patients [Group I: …
The Effects Of Sodium Selenite On The Antioxidative Defence Mechanism Of Human Hepatoma G_2 Cells, Kayahan Fişkin
The Effects Of Sodium Selenite On The Antioxidative Defence Mechanism Of Human Hepatoma G_2 Cells, Kayahan Fişkin
Turkish Journal of Medical Sciences
Human cells that live in biological harmony can become cancerous. As they do so, they engage in anaerobic respiration, undergo rapid cell growth and division according to nonmitotic rules, and important changes take place in their metabolism. We conducted several investigations in an effort to block or change the metabolic characteristics of cancer cells using a typical cancerous cell line. Specifically,we investigated the effects of sodium selenite (Na_2 SeO_ 3 ) on antioxidant enzyme activities and reduced glutathione levels in Hepatoma G_ 2 cells, cells that show rapid glutamine uptake and metabolism. A Hepatoma G_ 2 cell line was cultured …
The In Vitro Effects Of The Antimalarial Drug Primaquine, On The Activities Of Some Enzymes In Human Erythrocyte Lyzates, Meltem Müftüoğlu, Nazmi̇ Özer
The In Vitro Effects Of The Antimalarial Drug Primaquine, On The Activities Of Some Enzymes In Human Erythrocyte Lyzates, Meltem Müftüoğlu, Nazmi̇ Özer
Turkish Journal of Medical Sciences
The effect of primaquine on the enzymatic antioxidant defence mechanisms of erythrocytes were determined by measuring the activities of the below enzymes, before and after 30 minutes of incubation of hemolyzates with 1 mM primaquine at 37°C. Among the enzymes studied glucose-6- phosphate dehydrogenase seemed the most sensitive enzyme; 57 % and 78 % inhibition at zero (due to the binding of primaquine to NADP binding site) and 30 minutes of incubation (due to the reactive oxygen species), respectively. Catalase, superoxide dismutase, and glutathione-S-transferase were less sensitive to primaquine-derived reactive oxygen species: 33, 11, and 0 % inhibition at the …
The Effects Of Vitamin E On Catalase Activies In Various Rat Tissues, Gülinnaz Alper, Mehtap Çinar, Cenk Can, Gülriz Menteş, Biltan Ersöz, Akgün Evinç
The Effects Of Vitamin E On Catalase Activies In Various Rat Tissues, Gülinnaz Alper, Mehtap Çinar, Cenk Can, Gülriz Menteş, Biltan Ersöz, Akgün Evinç
Turkish Journal of Medical Sciences
Vitamin E and other antioxidants prevent or minimize oxidative damage in biological systems. The level of antioxidant defense sufficient to protect the organism from high free radical concentrations is one of the new horizons for Vitamin E research. The role of Vitamin E as a protective agent aganist oxidative stress was evaluated by meas-uring the antioxidant enzyme catalase (CAT) activities in different tissues of Swiss male albino rats aged two months. In this study, rats were divided to three groups; control (n=10), E 1 (n=5) which received 25 mg/kg. per day of Vitamin E, IM. for 10 days and E …
Alterations Of Erythrocyte And Plasma Lipid Peroxides As Well As Antioxidant Mechanism In Patients With Type Ii Diabetes Mellitus (Niddm), Yıldız Atamer, Yüksel Koçyi̇ği̇t, Aytaç Atamer, Nuriye Mete, Naime Canoruç, Gülten Toprak
Alterations Of Erythrocyte And Plasma Lipid Peroxides As Well As Antioxidant Mechanism In Patients With Type Ii Diabetes Mellitus (Niddm), Yıldız Atamer, Yüksel Koçyi̇ği̇t, Aytaç Atamer, Nuriye Mete, Naime Canoruç, Gülten Toprak
Turkish Journal of Medical Sciences
It has been maintained that free oxygen radicals (FOR) that occur in cell have important roles in the ethiopathogenesis of diabet, and that cell damage progressing as a result of their accumulation is responsible for the development of diabetic complications. In recent years, along with free oxygen radicals, antioxidant mechanisms and the substances influencing as free radical cleaner have been investigated in connection with diabet. With this respect, in a total of 30 patients with type II Diabetes Mellitus (NIDDM), 15 with diabetic retinopathy and 15 without diabetic retinopathy, and in 20 healthy subjects, we measured glutathione (GSH), catalase and …
Age-Related Alterations In Superoxide Dismutaseand Catalase Activities In Rat Brain, Gülinnaz Alper, Eser Y. Sözmen, Lütfiye Kanit, Gülriz Mentes, Biltan Ersöz, Fatma Z. Kutay
Age-Related Alterations In Superoxide Dismutaseand Catalase Activities In Rat Brain, Gülinnaz Alper, Eser Y. Sözmen, Lütfiye Kanit, Gülriz Mentes, Biltan Ersöz, Fatma Z. Kutay
Turkish Journal of Medical Sciences
Active oxygen species have been proposed to be involved in the aging process of the brain, therefore alterations in the activities of enzymes involved in the defense system against free radicals and other active species could substantially influence the aging process. This study is planned to determine the brain superoxide dismutase (SOD) and catalase activities which defend the components of brain cells against active oxygen species, in order to evaluate whether they play a role in the aging process. The activities of SOD and catalase were measured in the cerebral cortex and cerebellum of Swiss male albino rats aged either …