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Comparison Of Conventional Cytogenetic And Fluorescence In Situ Hybridization Results Of Prenatal Aneuploidy Screening, Zerri̇n Yilmaz, Özge Özalp, Ebru Tarim, Ayla Üçkuyu, Vural Dağli, Feri̇de İffet Şahi̇n

Turkish Journal of Medical Sciences

Background and Aim: Prenatal diagnosis of chromosome abnormalities is usually performed on amniotic fluid samples. Aneuploidy screening by fluorescence in situ hybridization (FISH), although not an alternative for conventional cytogenetics, has become a useful tool. Materials and Methods: We compared the results of FISH and conventional cytogenetic analysis applied on 51 amniotic fluid samples between January 2002 and December 2005, in our Cytogenetics Laboratory. Amniocytes were extracted using 5 cc of the sample, and direct FISH was applied using an AneuVysion Assay Kit (Vysis). The remaining fluid samples were used for chromosome analysis by conventional methods after long-term cell cultures. …