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Problems In Urinary Iodine Determination Methods And An Automated Kinetic Assay As A Solution, Mustafa Gültepe, Ömer Özcan, Osman Meti̇n İpçi̇oğlu, Kadi̇r Avşar

Turkish Journal of Medical Sciences

Accurate, fast and economical urinary iodine measurement is very important in diagnosing iodine deficiency disorders. The urinary iodine determination method using ammonium persulfate digestion based on the Sandell-Kolthoff reaction was optimized and modified to kinetic and automated assay. Ammonium persulfate digestion was performed at +95 ºC in a water-bath to ± 0.1 ºC precision. The performance of both the Sandell-Kolthoff reaction at 37 ºC and its kinetic measurement at 340 nm was tested in a random access automated analyzer. For method comparison, urinary iodine concentrations were measured using both the conventional chloric acid digestion method and the kinetic automated method …