Medicine and Health Sciences Commons^™

Increased Metabolic Rate In X-Linked Hypophosphatemic Mice, Linda K. Vaughn, Ralph A. Meyer, M. H. Meyer

Biomedical Sciences Faculty Research and Publications

Hyp mice are a model for human X-linked hypophosphatemia, the most common form of vitamin D-resistant rickets. It has previously been observed that Hyp mice have a greater food consumption per gram body weight than do normal mice. This led to the search for some alteration in metabolism in Hyp mice. We found that oxygen consumption was significantly higher in Hyp mice than in normal C57BL/6J mice and this was accompanied by an increased percentage of cardiac output being delivered to organs of heat production (liver and skeletal muscle), to the skin, and to bone and a decreased percentage to …

The Effects Of Triethyltin Bromide On Red Cell And Brain Cyclic Amp-Dependent Protein Kinases, Kevin R. Siebenlist, Fumito Taketa

Biomedical Sciences Faculty Research and Publications

Triethyltin bromide activates the cyclic AMP-dependent protein kinases of human red cell membranes and of bovine brain. Additions of 25-500 microM triethyltin to red cell ghosts resulted in enhanced phosphorylation of ghost proteins. When added to partially purified cyclic AMP-dependent protein kinases from red cell ghosts or bovine brain, stimulation of the phosphorylation of calf thymus histone was observed. The enhancement of kinase activity was due to release of catalytic subunits from the intact protein kinase. Brief exposure of the partially purified enzymes to triethyltin, followed by DE52 chromatography, resulted in elution profiles for regulatory and catalytic subunits that were …

Antibiotic Susceptibility Of Black-Pigmented Bacteroides Isolates From The Human Oral Cavity, Linda Jean Laatsch, Paul R. Hohenfeldt, William L. Kos

Biomedical Sciences Faculty Research and Publications

The minimal inhibitory concentrations of penicillin and six other antibiotics were determined for 66 oral black-pigmented Bacteroides isolates by using the National Committee for Clinical Laboratory Standards proposed standard agar dilution technique. These results plus iodometric determination of β-lactamase activity showed that oral isolates of black-pigmented Bacteroides are remaining relatively susceptible to commonly used antibiotics.

The Esophageal Propulsive Force: Esophageal Response To Acute Obstruction, Daniel H. Winship, F. Frank Zboralske

Biomedical Sciences Faculty Research and Publications

The response of the normal human esophagus to an obstructing intraluminal bolus was investigated and compared to the response evoked by transient intraluminal distention. A balloon, immobilized within the esophagus by external attachment to a force transducer, was inflated with from 3 to 25 ml of air for from 3 to 210 sec. Pressure phenomena occurring in the esophagus were simultaneously recorded from the body of the esophagus above and below the balloon.

Transient distention (5 sec or less) with small volumes (5 ml or less) often evoked a secondary peristaltic wave in the esophagus distal to the balloon, but …

Studies Of The Mechanism By Which Chronic Metabolic Acidosis Augments Urinary Calcium Excretion In Man, Jacob Lemann Jr., John R. Litzow, Edward J. Lennon

Biomedical Sciences Faculty Research and Publications

We carried out clearance studies in nine healthy adults and four patients with hypoparathyroidism before and after inducing stable metabolic acidosis with either NH₄Cl or acetazolamide. Clearances were repeated in seven normal subjects and three of the patients 3 days after stopping these agents.

During acidosis in the normal subjects, serum ultrafilterable calcium concentration rose significantly, but inulin clearance fell to a greater extent, so that the calculated filtered load of calcium fell significantly. Despite this, urinary calcium excretion rose. Urinary calcium excretion remained elevated in the recovery studies when the serum ultrafilterable calcium concentration and filtered load …

The Effect Of Treatment Of Acidosis On Calcium Balance In Patients With Chronic Azotemic Renal Disease, John R. Litzow, Jacob Lemann Jr., Edward J. Lennon

Biomedical Sciences Faculty Research and Publications

Small but statistically significant negative calcium balances were found in each of eight studies in seven patients with chronic azotemic renal disease when stable metabolic acidosis was present. Only small quantities of calcium were excreted in the urine, but fecal calcium excretion equaled or exceeded dietary intake. Complete and continuous correction of acidosis by NaHCO₃ therapy reduced both urinary and fecal calcium excretion and produced a daily calcium balance indistinguishable from zero.

Apparent acid retention was found throughout the studies during acidosis, despite no further reduction of the serum bicarbonate concentration. The negative calcium balances that accompanied acid retention …

Pneumonia Due To Mycoplasma In Gnotobiotic Mice: Ii. Localization Of Mycoplasma Pulmonis In The Lungs Of Infected Gnotobiotic Mice By Electron Microscopy, Avrum B. Organick, Kenneth A. Siegesmund, Irving I. Lutsky

Biomedical Sciences Faculty Research and Publications

Organick, Avrum B. (Marquette University School of Medicine, Milwaukee, Wis.), Kenneth A. Siegesmund, and Irving I. Lutsky. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J. Bacteriol. 92:1164–1176. 1966.—Lesions in lungs of gnotobiotic mice inoculated intranasally with Mycoplasma pulmonis were examined by electron microscopy after osmic acid fixation. At 1 week after infection, mycoplasma cells were found in large numbers in the bronchi at the surface of bronchial epithelial cells and, in smaller numbers, in the alveoli where active phagocytosis by polymorphonuclear leukocytes (PMN) occurred. …

Responses Of The Normal Human To Infusions Of Plasma From Patients With Graves' Disease, Claude D. Arnaud, Hans A. Kneubuhler, Virginia L. Seiling, Bruce K. Wightman, Norman H. Engbring

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Net Balance Of Acid In Subjects Given Large Loads Of Acid Or Alkali, Jacob Lemann Jr., Edward J. Lennon, A. David Goodman, John R. Litzow, Arnold S. Relman

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Production, Excretion, And Net Balance Of Fixed Acid In Patients With Renal Acidosis, A. David Goodman, Jacob Lemann Jr., Edward J. Lennon, Arnold S. Relman

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Presbyesophagus: Esophageal Motility In Nonagenarians, Konrad H. Soergel, F. Frank Zboralske, John R. Amberg

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Ultrastructure Of The Intranuclear Rodlet In Certain Nerve Cells, Kenneth A. Siegesmund, C. R. Dutta, C. A. Fox

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Reversible Changes In The Nem-Reactive--Sh Groups Of Hemoglobin On Oxygenation-Deoxygenation, Samuel A. Morell, Peg Hoffman, Valborg E. Ayers, Fumito Taketa

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Measurement Of Arterial Aging In Hypertensive Patients, Francois M. Abboud, John H. Huston

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Studies Of The Rate Of Disappearance Of Labeled Thyroxine From The Intravascular Compartment, Edward J. Lennon, Norman H. Engbring, William W. Engstrom

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Effects Of Aging And Degenerative Vascular Disease On The Measurement Of Arterial Rigidity In Man, Francois M. Abboud, John H. Huston

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Some Observations On The Fine Structure Of A Thermophilic, Acidophilic Alga, Walter G. Rosen, Kenneth A. Siegesmund

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Level Of Lactic Dehydrogenase Activity As An Indicator Of The Growth Of Influenza Virus In The Embryonate Egg, Richard Kelly, Donald Greiff

Biomedical Sciences Faculty Research and Publications

The relation, between time and the levels of lactic dehydrogenase activity of chorioallantoic fluids from embryonate eggs infected with the PR8 strain of influenza virus were determined quantitatively. The mean values, based on 10 determinations for each time interval, followed a sigmoid curve, with the greatest rates of change occurring between 48 and 72 hours after the inoculation of virus. The activities of the fluids from infected eggs at the 72nd hour or later were approximately 18 times higher than those from non-infected eggs. Based on the data above, a qualitative test for the presence of infection with influenza virus …

The Assay And Properties Of Labile Factor (Factor V), Armand J. Quick

Biomedical Sciences Faculty Research and Publications

Human oxalated plasma stored at 4° C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreement is not obtained with plasma stored at 37°C. The stability of labile factor is closely associated with ionized calcium. The addition of thrombin to fresh oxalated plasma causes an apparent hyperactivity of labile factor, but this is completely removed by adsorption with Ca₃(PO)₂. Oxalated plasma when …

The Effects Of X-Rays And Beta Rays (Tritium) On The Growth Of Rickettsia Mooseri And Rickettsia Akari In Embryonate Eggs, Donald Greiff, E. L. Powers, Walter E. Kisieleski, Henry Pinkerton

Biomedical Sciences Faculty Research and Publications

The growth of Rickettsia mooseri was accelerated and quantitatively increased in embryonate eggs containing tritium oxide at levels of 180, 90, and 45 mc./egg during the growth period. The eggs of a group containing 22.5 mc./egg showed only a slight increase in the rate of growth of organisms; the infections in the eggs of a group given 11.2 mc./egg did not differ significantly from those of the control group. On the other hand, growth of R. akari was inhibited in embryonate eggs containing tritium oxide at levels of 180, 90, and 45 mc./egg, and partially inhibited in groups containing 22.5 …

A Quantitative Study Of The Purkinje Cell Dendritic Branchlets And Their Relationship To Afferent Fibres, Clement A. Fox, John A. Barnard

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Hemophilia And Hemophiliacs, Armand J. Quick

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Infrared Photography, Leo C. Massopust Sr.

Biomedical Sciences Faculty Research and Publications

No abstract provided.

Pediatric References, Abraham B. Schwartz

Biomedical Sciences Faculty Research and Publications

No abstract provided.

On The Comparative Prothrombic Activity Of Human And Dog Blood, Armand J. Quick, Clara V. Hussey

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Chemical State Of The Calcium Reacting In The Coagulation Of Blood, Armand J. Quick, Mario Stefanini

Biomedical Sciences Faculty Research and Publications

1. The widely accepted theory that calcium participates in the coagulation mechanism in the form of Ca⁺⁺ and acts as a catalyst is not in accord with several important experimental findings:

(a) The anticoagulant action of sodium oxalate is much slower than the precipitation of ionized calcium as the oxalate salt.

(b) Sodium citrate begins to depress prothrombin activity at a concentration at which ionized calcium is still present. The inability of tricalcium phosphate to adsorb prothrombin from citrated plasma indicates that citrate forms a complex with prothrombin and it is postulated that prothrombin is …