Medicine and Health Sciences Commons^™

White Matter Integrity Abnormalities In Healthy Overweight Individuals Revealed By Whole Brain Meta-Analysis Of Diffusion Tensor Imaging Studies, Xiaodong Cheng, Wenchang Wang, Chen Sun, Yana Sun, Cong Zhou

Faculty, Staff and Student Publications

OBJECTIVE: This study aimed to conduct a coordinate-based meta-analysis (CBMA) to investigate white matter (WM) abnormalities in healthy individuals with overweight or obesity.

METHODS: A systematic literature search using Web of Science and PubMed datasets was performed. Original investigations that used diffusion tensor imaging (DTI) to explore fractional anisotropy (FA) differences between healthy overweight/obese individuals and normal weight controls were collected. The meta-analysis was conducted using the seed-based

RESULTS: The analysis included five studies comprising 232 overweight/obese individuals and 219 healthy normal weight controls. The findings showed that overweight/obese individuals exhibited reduced fractional anisotropy (FA) in specific regions, namely, the …

Suppressing A Blocked Balance Recovery Step: A Novel Method To Assess An Inhibitory Postural Response, David A. E. Bolton, Charlie C. Baggett Iv, Chase A. Mitton, Sara A. Harper, James K. Richardson

Kinesiology and Health Science Faculty Publications

Stepping to recover balance is an important way we avoid falling. However, when faced with obstacles in the step path, we must adapt such reactions. Physical obstructions are typically detected through vision, which then cues step modification. The present study describes a novel method to assess visually prompted step inhibition in a reactive balance context. In our task, participants recovered balance by quickly stepping after being released from a supported forward lean. On rare trials, however, an obstacle blocked the stepping path. The timing of vision relative to postural perturbation was controlled using occlusion goggles to regulate task difficulty. Furthermore, …

Re-Stratification Of Patients With Copy-Number Low Endometrial Cancer By Clinicopathological Characteristics, Li Liwei, Li He, Dai Yibo, Zhao Luyang, Shen Zhihui, Kang Nan, Shen Danhua, Wang Junzhu, Wang Zhiqi, Wang Jianliu

Faculty, Staff and Student Publications

Objective: To stratify patients with copy-number low (CNL) endometrial cancer (EC) by clinicopathological characteristics.

Methods: EC patients who underwent surgery between June 2018 and June 2022 at Peking University People's Hospital were included and further classified according to TCGA molecular subtyping: POLE ultramutated, microsatellite instability high (MSI-H), CNL, and copy-number high (CNH). Clinicopathological characteristics and prognosis of CNL patients were retrospectively reviewed. The Cox proportional hazards regression model was applied to perform univariate and multivariate analysis, and independent risk factors were identified. Differentially expressed genes (DEGs) according to overall survival (OS) were screened based on the transcriptome of CNL cases …

Co-Infection And Co-Localization Of Kaposi Sarcoma-Associated Herpesvirus And Epstein-Barr Virus In Hiv-Associated Kaposi Sarcoma: A Case Report, Peter Julius, Guobin Guobin, Stepfanie Siyumbwa, Jane Musumali, For Yue Tso, Owen Ngalamika, Trevor Kaile, Fred Maate, Phyllis Moonga, John T. West, Peter Angeletti, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIVpositive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcomaassociated herpesvirus (KSHV) infection. In this case report, we present a rare occurrence of co-infection and co-localization of KSHV and Epstein-Barr virus (EBV) in KS arising from the conjunctiva, which, to our knowledge, has not been reported previously. Immunohistochemistry (IHC), DNA polymerase chain reaction (PCR), and EBV-encoded RNA in situ hybridization (EBER-ISH) were utilized to demonstrate the presence of KSHV and EBV infection in the ocular KS lesion. Nearly all KSHV-positive …

Statistical Modeling Of Extracellular Vesicle Cargo To Predict Clinical Trial Outcomes For Hypoplastic Left Heart Syndrome, Jessica R Hoffman, Hyun-Ji Park, Sruti Bheri, Manu O Platt, Joshua M Hare, Sunjay Kaushal, Judith L Bettencourt, Dejian Lai, Timothy C Slesnick, William T Mahle, Michael E Davis

Faculty, Staff and Student Publications

Cardiac-derived c-kit+ progenitor cells (CPCs) are under investigation in the CHILD phase I clinical trial (NCT03406884) for the treatment of hypoplastic left heart syndrome (HLHS). The therapeutic efficacy of CPCs can be attributed to the release of extracellular vesicles (EVs). to understand sources of cell therapy variability we took a machine learning approach: combining bulk CPC-derived EV (CPC-EV) RNA sequencing and cardiac-relevant

Age-Associated Spinal Stenosis In The Turquoise Killifish, Su-Hyeon Cho, Seongsin Lee, Jae-Il Park, Yoon La Yang, Song-Rae Kim, Juhee Ahn, Hoibin Jeong, Hye-Yeon Jung, Nayoung Gwak, Kil-Nam Kim, Yumi Kim

Faculty, Staff and Student Publications

Aging triggers spinal degeneration, including common spinal stenosis, which causes back and leg pain in older individuals, significantly impacting their quality of life. Here, we explored aging traits in turquoise killifish spines, potentially offering a model for age-linked spinal stenosis in humans. Aged turquoise killifish exhibited body shape deformation and increased vertebral collapse, which was further accelerated by spawning. High-resolution CT scans revealed suppressed cortical bone thickness and hemal arch area in vertebrae due to spawning, and osteophyte formation was observed in both aged and breeding fish populations. Scale mineralization mirrored these changes, increasing with age but being suppressed by …

Focalizing Regions Of Biomarker Relevance Facilitates Biomarker Prediction On Histopathological Images, Jiefeng Gan, Hanchen Wang, Hui Yu, Zitong He, Wenjuan Zhang, Ke Ma, Lianghui Zhu, Yutong Bai, Zongwei Zhou, Alan Yullie, Xiang Bai, Mingwei Wang, Dehua Yang, Yanyan Chen, Guoan Chen, Joan Lasenby, Chao Cheng, Jia Wu, Jianjun Zhang, Xinggang Wang, Yaobing Chen, Guoping Wang, Tian Xia

Faculty, Staff and Student Publications

Image-based AI has thrived as a potentially revolutionary tool for predicting molecular biomarker statuses, which aids in categorizing patients for appropriate medical treatments. However, many methods using hematoxylin and eosin-stained (H&E) whole-slide images (WSIs) have been found to be inefficient because of the presence of numerous uninformative or irrelevant image patches. In this study, we introduced the region of biomarker relevance (ROB) concept to identify the morphological areas most closely associated with biomarkers for accurate status prediction. We actualized this concept within a framework called saliency ROB search (SRS) to enable efficient and effective predictions. By evaluating various lung adenocarcinoma …

Restructuring And Serving Web-Accessible Streamflow Data From The Noaa National Water Model Historic Simulations, J Michael Johnson, David L Blodgett, Keith C Clarke, Jon Pollak

Faculty, Staff and Student Publications

In 2016, the National Oceanic and Atmospheric Administration deployed the first iteration of an operational National Water Model (NWM) to forecast the water cycle in the continental United States. With many versions, an hourly, multi-decadal historic simulation is made available to the public. In all released to date, the files containing simulated streamflow contain a snapshot of model conditions across the entire domain for a single timestep which makes accessing time series a technical and resource-intensive challenge. In the most recent release, extracting a complete streamflow time series for a single location requires managing 367,920 files (~16.2 TB). In this …

Evaluating The Impact Of An Mhealth Platform For Managing Acute Postoperative Dental Pain: Randomized Controlled Trial, Bunmi Tokede, Alfa Yansane, Ana Ibarra-Noriega, Joanna Mullins, Kristen Simmons, Nicholas Skourtes, Urvi Mehta, Sayali Tungare, David Holmes, Joel White, Muhammad Walji, Elsbeth Kalenderian

Faculty, Staff and Student Publications

BACKGROUND: Postoperative dental pain is pervasive and can affect a patient's quality of life. Adopting a patient-centric approach to pain management involves having contemporaneous information about the patient's experience of pain and using it to personalize care.

OBJECTIVE: In this study, we evaluated the use of a mobile health (mHealth) platform to collect pain-related patient-reported outcomes over 7 days after the patients underwent pain-inducing dental procedures; we then relayed the information to the dentist and determined its impact on the patient's pain experience.

METHODS: The study used a cluster-randomized experimental study design with an intervention arm where patients were prompted …

Actinide-Lanthanide Single Electron Metal-Metal Bond Formed In Mixed-Valence Di-Metallofullerenes, Yingjing Yan, Laura Abella, Rong Sun, Yu-Hui Fang, Yannick Roselló, Yi Shen, Meihe Jin, Antonio Rodríguez-Fortea, Coen De Graaf, Qingyu Meng, Yang-Rong Yao, Luis Echegoyen, Bing-Wu Wang, Song Gao, Josep M Poblet, Ning Chen

Faculty, Staff and Student Publications

Understanding metal-metal bonding involving f-block elements has been a challenging goal in chemistry. Here we report a series of mixed-valence di-metallofullerenes, ThDy@C2n (2n = 72, 76, 78, and 80) and ThY@C2n (2n = 72 and 78), which feature single electron actinide-lanthanide metal-metal bonds, characterized by structural, spectroscopic and computational methods. Crystallographic characterization unambiguously confirmed that Th and Y or Dy are encapsulated inside variably sized fullerene carbon cages. The ESR study of ThY@D3h(5)-C78 shows a doublet as expected for an unpaired electron interacting with Y, and a SQUID magnetometric study of ThDy@ …

Determining The Ideal Concentrations Of Ethanol And Propylene Glycol In Ethosomes For Transdermal Delivery Of Vitamin D3, Rebecca Conner

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Vitamin D₃ is an important chemical in the human body, however, many Americans have low levels of this vitamin. There are plenty of oral supplementations for Vitamin D₃ deficiency, but those of older age and busy schedules may struggle to meet the minimum requirement. A recently developed ethosomal transmembrane delivery system (Touitou, 2000), similar to liposomes but also containing ethanol, allows users to apply a gel dermally and have the desired drug or active ingredient reach the bloodstream faster. However, there is considerable variation in the concentration of ethanol and permeation enhancers used. Using an affordable method to …

Development Of A Novel Serum Formulation For The Treatment Of C. Acnes, Ariana G. Ngue

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Acne impacts 85% of people between 12-24 years of age. Even after puberty, acne continues to impact a large portion of the population. The treatments are diverse and include prescription drugs. Through this research, a novel formulation was created for the skin to treat acne. This formulation incorporates salicylic acid, a non-prescription acne treatment, as well as a polymeric carbohydrate (hyaluronic acid) known to be a component of skin and involved in the healing of skin. Through an internship with Island Lux Essentials, a company devoted to making all natural body care products, a novel serum formulation was created to …

Liposome Synthesis And Evaluation In The Hek-293 Cell Line, Christine Skibinski

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Liposomes were synthesized using the thin film method. A lyophilized power of egg-derived phosphatidylcholine, stearylamine, and cholesterol were added to ethanol and dried under argon to form a lipid cake. The lipid cake was rehydrated with dPBS and sonicated at 60°C forming a heterogenous batch of liposomes. Our results revealed the average size of the liposomes, determined by Dynamic Light Scattering, was approximately 223.1nm, while demonstrating a weakly positive zeta-potential of 1.9± 8.07mv. Next, we tested the antitumor action of the liposomes in the HEK-293 cell line via an MTT assay. We observed that the liposomes were able to inhibit …

Physical Activity And Mental Health Of Youth During The Covid-19 Pandemic, Amber Watson, Alicia Fedewa, Clair Tischner

International Journal of Physical Activity and Health

The COVID-19 Pandemic has globally impacted children’s physical activity and mental health outcomes since the transition to remote learning (Štveráková, et al., 2021). Children and adults may encounter negative mental health related consequences, higher stress, and a significant lack of physical activity while in physical and social isolation (Clemente-Suárez, 2022). In particular, school-aged children have experienced a significant reduction in physical activity since transitioning to remote learning and do not have sufficient opportunities to make up for this loss while engaged in remote learning (Štveráková, et al., 2021). Increased physical activity has been consistently correlated with positive mental health outcomes …

In Silico Prediction Of Sodium-Glucose Co-Transporter-2 (Sglt2) Inhibition Activity By Allium Fistulosum Compound Based On Skelspheres Molecular Descriptor, Rudy Heryanto, Aryo Tedjo, Dimas Noor

Indonesian Journal of Medical Chemistry and Bioinformatics

The Sodium-Glucose co-transporter-2 (SGLT2) inhibitor represents a novel agent for the treatment of type 2 diabetes. Drugs of this class function by inhibiting glucose reabsorption in the kidneys, thereby controlling blood glucose levels. It is known that SGLT2 inhibitors activate the AMPK signaling pathway by increasing the expression and activity of AMP-activated protein kinase (AMPK). In vivo tests have demonstrated that ethanolic and aqueous extracts of Welsh onion leaves (Allium fistulosum L) can reduce body weight, liver weight, adipocyte size, and enhance AMP-activated protein kinase (AMPK) expression. In this study, the inhibitory activity (IC50) of compounds within Allium fistulosum against …

Adaptive Design Of Mrna-Loaded Extracellular Vesicles For Targeted Immunotherapy Of Cancer, Shiyan Dong, Xuan Liu, Ye Bi, Yifan Wang, Abin Antony, Daeyong Lee, Kristin Huntoon, Seongdong Jeong, Yifan Ma, Xuefeng Li, Weiye Deng, Benjamin R Schrank, Adam J Grippin, Jonghoon Ha, Minjeong Kang, Mengyu Chang, Yarong Zhao, Rongze Sun, Xiangshi Sun, Jie Yang, Jiayi Chen, Sarah K Tang, L James Lee, Andrew S Lee, Lirong Teng, Shengnian Wang, Lesheng Teng, Betty Y S Kim, Zhaogang Yang, Wen Jiang

Faculty, Staff and Student Publications

The recent success of mRNA therapeutics against pathogenic infections has increased interest in their use for other human diseases including cancer. However, the precise delivery of the genetic cargo to cells and tissues of interest remains challenging. Here, we show an adaptive strategy that enables the docking of different targeting ligands onto the surface of mRNA-loaded small extracellular vesicles (sEVs). This is achieved by using a microfluidic electroporation approach in which a combination of nano- and milli-second pulses produces large amounts of IFN-γ mRNA-loaded sEVs with CD64 overexpressed on their surface. The CD64 molecule serves as an adaptor to dock …

Braf D594a Mutation Defines A Unique Biological And Immuno-Modulatory Subgroup Associated With Functional Cd8+ T Cell Infiltration In Colorectal Cancer, Wenjing Li, Chenyi Zhao, Wenhui Li, Yang Gong, Kaili Ma, Yujie Lu, Xiaowei Liu, Lianjun Zhang, Feng Guo

Faculty, Staff and Student Publications

BACKGROUND: BRAF non-V600 mutation occupies a relatively small but critical subset in colorectal cancer (CRC). However, little is known about the biological functions and impacts of BRAF class III mutation in CRC. Here, we aim to explore how D594A mutation impacts on biological behaviors and immune related signatures in murine CRC cells.

METHODS: BRAF V600E (class I), G469V (class II) and D594A (class III) mutant cell lines were established based on MC38 cells. The biological behaviors of cells were evaluated in respect of cell growth, cell proliferation, cell apoptosis, cell migration and invasion by the methods of colony-forming assay, CCK-8 …

Advances And Challenges In Bioinformatics And Biomedical Engineering: Iwbbio 2020, Olga Valenzuela, Mario Cannataro, Irena Rusur, Jianxin Wang, Zhongming Zhao, Ignacio Rojas

Faculty, Staff and Student Publications

This Supplement issue, presents five research articles which are distributed, mainly due to the subject they address, from the 8th International Work-Conference on Bioinformatics and Biomedical Engineering (IWBBIO 2020), which was held on line, during September, 30th-2nd October, 2020. These contributions have been chosen because of their quality and the importance of their findings. Those contributions were then invited to participate in this supplement for the following journals of BMC: BMC Bioinformatics and BMC Genomics. In the present Editorial in BMC journal, we summarize the contributions that provide a clear overview of the thematic areas covered by the IWBBIO conference, …

Effect Of Exogenous Melatonin On Metabolic Profile And Reproductive Performance In Undernourished Suckling Merino Ewes In The Early Postpartum Period, Bariş Güner, Buse Öztürk, Aslihan Ayalp, İhsan Kisadere, Nevzat Saat, Muhi̇tti̇n Zengi̇n, Recai̇ Kulaksiz, Şükrü Meti̇n Pancarci

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine whether serum metabolite and hormones of ewes with low body condition scores subjected to the melatonin plus progesterone-based protocol (MPE) would be similar to a single progesterone-based protocol (PE) and whether melatonin ear implant supplementation could increase pregnancy rate. Ninety suckled Merino ewes with singleton lamb were equally allocated in two experimental groups (MPE, PE) and a control group (CON) on days 53 ± 3 (D0) postpartum. Ewes in MPE group were subjected to exogenous melatonin plus progesterone-based protocol [Melatonin (day 0) + P4 insertion (day 35) + 500 IU eCG (day 42)]. Ewes in …

Comparison Of Carcass Characteristics, Meat Quality, And Fatty Acid Composition In Slowand Fast-Growing Broilers At Different Slaughter Weights, Büşra Yaranoğlu, Hi̇lal Çapar Akyüz, Esi̇n Ebru Onbaşilar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to compare the slaughter, meat quality characteristics, and fatty acid composition of fast-growing (FAG) and slow-growing (SWG) broilers at different slaughter weights. In the experiments, a total of 90 carcasses were used: 45 SWG (Hubbard-Isa Red JA) and 45 FAG broilers (Ross 308) with 15 carcasses from each slaughter weight group (1500 ± 50 g, 2000 ± 50 g, 2500 ± 50 g). Hot carcass yield and cold carcass yield detected in the FAG broilers were higher than in SGW broilers (p < 0.001). As the weight of slaughter increased, hot carcass and cold carcass yield increased (p < 0.001). The breast percentage was significantly lower in the SWG broilers compared to the FAG broilers (p < 0.001). In contrast, thigh, wing, and abdominal fat percentages were higher in the SWG broilers (p < 0.001). The breast percentage increased as the slaughter weight increased (p < 0.001). SWG broilers had significantly lower pH, except for the pH 24 of the thigh meat (p < 0.01). The L*, a*, and b* values of the SWG broilers were lower than the FAG broilers (p < 0.01). In terms of a* values determined initially and at the 24th h in the breast and thigh meat, the 1500 g slaughter weight group had by far the highest value (p < 0.01). SWG broilers had significantly lower values in terms of cooking loss, water-holding capacity, and drip loss (p < 0.001). C18:2ω6 was detected at higher amounts in the FAG broilers (p < 0.05). The FAG broilers had higher PUFA, desired fatty acids, PUFA/SFA, and thrombogenic index values (p < 0.05). The results show that FAG broilers can meet the strong worldwide demand for meat quantity and quality. Slaughter weight changes only affected the quantity of the meat.

In Vitro Determination Of The Anthelmintic Potential Of Arum Rupicola Boiss Var. Rupicola, Tuğrul Atalay, Kader Yildiz

Turkish Journal of Veterinary & Animal Sciences

Arum rupicola Boiss var. rupicola is a preferred ingredient in traditional medicine in various countries, including Türkiye. The current study aims to establish the antiparasitic effect of A. rupicola Boiss var. rupicola on the eggs and larvae of T. canis in vitro. An ovicidal effect was not detected in the plant extracts, and embryogenesis in the T. canis eggs was not affected by the leaves' ethanolic extract and the distilled water extract of the rhizome during the incubation periods (6, 12, and 24 h). The larvicidal activity was observed in both extracts on the T. canis larvae after hatching. All …

Oncolytic Virus M1 Functions As A Bifunctional Checkpoint Inhibitor To Enhance The Antitumor Activity Of Dc Vaccine, Jia Dan, Jing Cai, Yingqian Zhong, Chaoqun Wang, Shanyu Huang, Ying Zeng, Zhen Fan, Cuiying Xu, Linyi Hu, Jiayu Zhang, Jun Hu, Ying Liu, Xingwen Su, Wenbo Zhu, Guangmei Yan, Jiankai Liang, Yuan Lin

Faculty, Staff and Student Publications

Although promising, dendritic cell (DC) vaccines still provide limited clinical benefits, mainly due to the immunosuppressive tumor microenvironment (TME) and the lack of tumor-associated antigens (TAAs). Oncolytic virus therapy is an ideal strategy to overcome immunosuppression and expose TAAs; therefore, they may work synergistically with DC vaccines. In this study, we demonstrate that oncolytic virus M1 (OVM) can enhance the antitumor effects of DC vaccines across diverse syngeneic mouse tumor models by increasing the infiltration of CD8+ effector T cells in the TME. Mechanically, we show that tumor cells counteract DC vaccines through the SIRPα-CD47 immune checkpoint, while OVM can …

Impairment Of Serine Transport Across The Blood-Brain Barrier By Deletion Of Slc38a5 Causes Developmental Delay And Motor Dysfunction, Inna Radzishevsky, Maali Odeh, Oded Bodner, Salman Zubedat, Lihi Shaulov, Maxim Litvak, Kayoko Esaki, Takeo Yoshikawa, Bella Agranovich, Wen-Hong Li, Alex Radzishevsky, Eyal Gottlieb, Avi Avital, Herman Wolosker

Faculty, Staff and Student Publications

Brain L-serine is critical for neurodevelopment and is thought to be synthesized solely from glucose. In contrast, we found that the influx of L-serine across the blood-brain barrier (BBB) is essential for brain development. We identified the endothelial Slc38a5, previously thought to be a glutamine transporter, as an L-serine transporter expressed at the BBB in early postnatal life. Young Slc38a5 knockout (KO) mice exhibit developmental alterations and a decrease in brain L-serine and D-serine, without changes in serum or liver amino acids. Slc38a5-KO brains exhibit accumulation of neurotoxic deoxysphingolipids, synaptic and mitochondrial abnormalities, and decreased neurogenesis at the dentate gyrus. …

Author Correction: Global Proteomic Identifies Multiple Cancer-Related Signaling Pathways Altered By A Gut Pathobiont Associated With Colorectal Cancer, Ewa Pasquereau-Kotula, Giulia Nigro, Florent Dingli, Damarys Loew, Patrick Poullet, Yi Xu, Scott Kopetz, Jennifer Davis, Lucie Peduto, Catherine Robbe-Masselot, Philippe Sansonetti, Patrick Trieu-Cuot, Shaynoor Dramsi

Faculty, Staff and Student Publications

No abstract provided.

Evaluation Of The Effects Of Ghrelin And Metformin On Sperm Parameters, Testosterone Hormones, And Immunohistochemical And Immunofluorescent Markers In An Experimental Diabetes Model, Saadet Belhan, Zübeyi̇r Huyut, Serkan Yildirim, Özlem Ergül Erkeç, Uğur Özdek

Turkish Journal of Veterinary & Animal Sciences

Testicular dysfunction, a severe secondary complication of diabetes, negatively affects reproductive health. Ghrelin is a peptide that plays a role in many metabolic events, such as stimulating growth hormones and regulating appetite, blood pressure, and reproduction. Metformin has antiinflammatory and antioxidant properties and is widely used to treat type 2 diabetes. Therefore, in this study, to investigate the effects of ghrelin and metformin on testicular function in experimental diabetic rats given streptozotocin, placed on a high-fat diet, and that had their sperm parameters evaluated, testosterone hormone, caspase 3 and iNOS expression, and histopathological examinations were performed. In total, 38 Wistar …

Radiographic Pelvimetry In Scottish Fold Cats: Sex-Related Differences, Osman Yilmaz, Davut Koca, Talha Avcilar

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to obtain osteometric measurements of the pelvis of Scottish Fold cats using X-ray images and reveal the biometric differences of these measurement values in terms of sexual dimorphism. Radiographic images of the pelvis in the laterolateral and ventrodorsal direction were used in male and female Scottish Fold cats aged 2-4 years with dystocia and eutocia. The measurements of 18 osteometric parameters and 36 parameters, such as the height/width ratio of the cat pelvis, were taken, and statistical analysis of the measurements was initiated. When the pelvimetric data were examined, it was observed that all of …

Determining The Protective Effect Of A Boron Adjuvanted Vaccine With An Experimental Infection Model In Mice, Zafer Sayin, Ali̇ Uslu, Özgür Özdemi̇r, Abdullah Başoğlu, Asli Balevi̇, Uçkun Sai̇t Uçan, Zeki̇ Aras, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Boron is a required trace element for plants, humans, and animals and it has significant influence over biological functions and the immune system. Vaccines have aided humans in the control of many contagious diseases around the world. The success of global immunization campaigns may be possible with the development of effective and low-cost vaccines and adjuvants. For this reason, the present study aimed to determine the adjuvant activity of easily accessible boron compounds. In this study, six different vaccines were prepared, including inactive Staphylococcus aureus bacteria, boric acid, aluminum hydroxide, montanide ISA 50, and ISA 206, ISA 50 + boric …

Preantral Follicle Morphometry And Ultrastructure Of Antral Follicles In Anatolian Water Buffalo, Duygu Baki̇ Acar, Muhammed Kürşad Bi̇rdane, Çi̇ğdem Tokyol, Nermi̇n Nüket Göçmen Karabeki̇r, Hati̇ce Mürvet Hayran, Erhan Özenç, Fatma Aktepe, Ebubeki̇r Yazici, İlknur Pi̇r Yağci, Mehmet Uçar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate quantitative and morphometric analyses of preantral follicles and the ultrastructural characteristics of antral follicles in different oestrous cycle stages in Anatolian water buffaloes. Twenty-four ovaries collected from twelve slaughtered Anatolian water buffaloes were classified macroscopically as luteal or follicular stages. The ovaries were prepared for histological examination (Hematoxylin-eosin staining), and primordial, primary, and secondary follicle numbers were calculated, and the diameters of oocytes, follicles, and nuclei were measured under a light microscope with a micrometre. The theca and granulosa cells of antral follicles were observed under a transmission electron microscope. The mean number of preantral …

The Effect Of Using Different Litter Materials In Broiler Rearing On Performance, Carcass Yield, Antioxidant Status, Some Litter Parameters, And Coccidiosis Oocysts, Gökhan Şen, Mehmet Naci̇ Oktay, Şevket Evci̇, Sami̇ Gökpinar, Ali̇ Şenol

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of various mixtures of different litter materials containing wood shaving and barley straw on body weight gain, feed consumption, feed efficiency, carcass yield, antioxidant state, litter quality, and coccidio oocysts in broilers rearing. For this purpose, a total of 120, 0-day-old Ross 308 broilers were assigned to 3 groups with 4 replicates. Wood shaving, barley straw, and wood shaving + barley straw group had 10 chicks in each replicate, a total of 40 chicks in each group. When the overall period of the study was examined, it was determined that …

Immunoreactivity Of Infiltrating Macrophages During Gastric Ulcer Healing In Rats, Erkmen Tuğrul Epi̇kmen, Emrah İpek, Recai̇ Tunca

Turkish Journal of Veterinary & Animal Sciences

This study investigated a timeline of changes in the immunoreactivity of infiltrating macrophages during gastric ulcer healing. To this end, gastric tissue samples were obtained from an acetic acid-induced gastric ulcer model in rats on the 1st, 3rd, 7th, and 14th days of ulcer induction. Ulcerated gastric tissues were subjected to histologic and immunohistochemical evaluations for macrophage subtypes. The number of Iba-1+ macrophages in the gastric ulcer area significantly increased on day 3, reaching a maximum number on day 7, followed by a decrease on day 14. No Gal-3+ macrophages were seen in the gastric ulcer area until day 14. …