Medicine and Health Sciences Commons^™

Peningkatan Berat Akar, Berat Nodul Efektif Dan Hasil Hijauan Legum Dengan Pemberian Molibdenum Dan Inokulasi Rhizobium (Increasing Root Weight, Nodule Weight Effectively And Fresh Forage Of Legumes By Adding Molybdenum Fertilizer And Rizobium Inoculation, Iin Susilawati, Nyimas Popi Indriani, Herryawan Kemal Mustofa, Ana Rochana Tarmidi

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini dilakukan untuk mengkaji pengaruh pemberian molibdenum dan inokulasi rhizobium terhadap berat akar, berat nodul efektif dan hasil  hijauan legum. Rancangan percobaan menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 3 faktor (4 x 2 x 2) dengan 2 kali ulangan. Faktor pertama yaitu jenis legum terdiri atas 4 jenis yaitu Siratro (Macroptilium atropurpureum), Kudzu (Pueraria phaseoloides), Sentro (Centrosema pubescens) dan Kalopo (Calopogonium mucunoides), faktor kedua molibdenum terdiri atas  2 taraf (m0 = tanpa molibdenum, m1 = dengan molibdenum, 6 g kg-1 benih) dan faktor ketiga inokulasi rhizobium terdiri atas dua taraf (r0 = tanpa inokulasi rhizobium; r1 = dengan …

Respon Broiler Terhadap Pemberian Ransum Mengandung Dedak Padi Fermentasi Oleh Kapang Aspergillus Ficuum (Response Of Broiler Fed On Diet Containing Fermented Rice Bran By Aspergillus Ficuum), Siti Wahyuni H.S., Dwi Cipto Budinuryanto, H. Supratman

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian bertujuan untuk mengetahui respon broiler terhadap pemberian ransum mengandung dedak padi hasil fermentasi oleh Aspergillus ficuum yang diukur melalui konsumsi ransum, pertambahan bobot badan, konversi ransum, persentase karkas dan imbangan efisiensi protein.  Penelitian menggunakan 300 ekor ayam broiler final stock Arbor Acres yang dibagi ke dalam 30 plot.  Ransum disusun iso-protein dan iso-energi dengan kandungan protein kasar 22% dan energi metabolis 3100 kkal/kg.  Penelitian dilakukan secara eksperimen menggunakan Rancangan Acak Lengkap , terdiri atas 6 ransum sebagai perlakuan setiap perlakuan diulang 5 kali.  Ransum perlakuan terdiri atas R0 (0% dedak padi fermentasi), R1 (5% dedak padi fermentasi), R2 (10% …

Studi Pembuatan Kompleks Mineral-Minyak Dan Efek Penggunaannya Dalam Ransum Terhadap Fermentabilitas Dan Kecernaan (In Vitro) (Study Of Oil-Mineral Complex And It Effects On In Vitro Fermentability And Digestibility), U. Hidayat Tanuwiria, Dwi Cipto Budinuryanto, S. Darodjah, Wendry Setiyadi Putranto

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum yang mengandung berbagai kompleks minyak-mineral in vitro.  Penelitian dilakuan secara eksperimen dengan Rancangan Acak Lengkap (RAL) empat perlakuan dan lima ulangan. Percobaan pertama terdiri atas R1 = Ransum lengkap + 5% Minyak Jagung, R2 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Jagung, R3 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Kacang Tanah, R4 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Ikan. Perlakuan kedua : R1 = Ransum lengkap + 5% Minyak Kacang Tanah. R2 = Ransum lengkap + 5% Kompleks I-Minyak Jagung, R3 = Ransum lengkap + 5% Kompleks I-Minyak Kacang Tanah, …

Isolasi Yeasts Dari Daging Dan Potensinya Sebagai Agen Biopreservasi Dan Pewarna Makanan (Isolation Of Yeasts From Meat And Its Potential As Bio Preservation Agent And Food Coloring), Wendry Setiyadi Putranto, Roostita L. Balia, Obin Rachmawan, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

Yeasts merupakan mikroba potensial untuk dikembangkan sebagai penghasil komponen bioaktif seperti enzim, antimicrobial agent sebagai biopreservasi, serta pengahasil pewarna makanan (pigment).  Penelitian merupakan studi ekploratif potensi yeasts yang diisolasi dari daging ayam dan sapi. Ditemukan isolate yeasts yang memiliki karakteristik spesifik dengan warna merah.  Jumlah total yeasts pada daging ayam 2.105 cfu/gram sedangkan pada daging sapi 1.105 cfu/gram. Hasil pemurnian isolate  diperoleh dua isolate yang memiliki kemampuan daya hambat yang terhadap Pseudomonas aerugenes dan Staphylococus aureus serta tidak meiliki aktivitas penghambatan terhadap Escherichia coli dan Candida albicans. pH akhir media selama fermentasi 25 0C, 48 jam adalah 5,5 dan 5,6 …

Karakteristik Stirred Yoghurt Mangga (Mangifera Indica) Dan Apel (Malus Domestica) Selama Penyimpanan (Characteristics Of Mango (Mangifera Indica) And Apple (Malus Domestica) Sittted Yoghurt During Storage), Eka Wulandari, Wendry Setiyadi Putranto

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan jus buah mangga dan apel terhadap  keasaman, total bakteri dan sineresis stirred yoghurt mangga dan apel yang dihasilkan dan selama proses penyimpanan. Penelitian ini dilakukan dengan tiga perlakuan yaitu stirred yoghurt mangga, stirred yoghurt apel dan kontrol. Stirred yoghurt yang dihasilkan disimpan selama 7 hari. Hasil penelitian menunjukkan stirred yoghurt mangga (210 menit, pH=4,3) dan apel (210 menit, pH=4,2) terkoagulasi lebih cepat dan pada pH yang lebih rendah dibandingkan dengan control (250 menit, pH=4,4). Selama penyimpanan kadar asam laktat dan jumlah bakteri asam laktat lebih tinggi pada stirred yoghurt mangga dan apel dibandingkan …

Deteksi Jumlah Bakteri Total Dan Koliform Pada Lumpur Hasil Ikutan Pembentukan Gasbio Dari Feses Sapi Perah (Detection Total Bacteria Amount And Coliform In Sludge Biogas From Dairy Cattle Feces), Yuli Astuti Hidayati, Ellin Harlia, Eulis Tanti Marlina

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini dilaksanakan di laboratorium Mikrobiologi dan Pengolahan Limbah Fakultas Peternakan universitas padjadjaran. Tujuan penelitian ini adalah untuk mengetahui jumlah total bakteri dan koliform pada lumpur hasil ikutan pembentukan gasbio dari feses sapi perah. Penelitian dilakukan dengan metode eksplorasi pada peternak sapi perah yang membuat instalasi gasbio di desa Haurngombong, Kecamatan Pamulihan-Sumedang. Hasil penelitian menunjukkan jumlah bakteri total adalah 41,82 x 1012 cfu/ml lumpur, sedangkan jumlah 8,23 MPN/ml lumpur. Kata kunci : bakteri total, koliform, lumpur, gasbio, feses sapi perah.

Peran Usaha Perbibitan Dalam Pengembangan Ternak Sapi Perah Di Indonesia (The Role Of Breeding Farm On Dairy Cattle Development In Indonesia), Achmad Firman, Sri Bandiati Komar Prajoga

Jurnal Ilmu Ternak Universitas Padjadjaran

Usaha perbibitan pada peternakan sapi perah memegang peranan penting dalam penyediaan stock bibit sapi perah. Dalam kerangka budidaya sapi perah, perbibitan atau breeding merupakan bagian yang tidak terpisahkan dari siklus feeding, breeding, dan management. Ke tiga faktor tersebut sangat penting dalam usahaternak sapi perah. Tujuan dari kajian ini adalah untuk mengetahui sejauhmana peran perbibitan dan program-program perbibitan dalam penyediaan ternak pengganti, dan untuk mengetahui berapa besar investasi yang diperlukan dalam usaha perbibitan sapi perah. Metode yang dilakukan pada kajian ini adalah kajian pustaka yang dititikberatkan pada usaha perbibitan sapi perah. Hasil penelitian menunjukkan bahwa berdasarkan hasil mapping terhadap peran pelaku …

Pengaruh Penggunaan Asam Sulfat (H2so4) Dan Asam Formiat (Hcooh) Pada Proses Pikel Terhadap Kualitas Kulit Jadi (Leather) Domba Garut (The Effect Of Sulfuric Acid (H2so4) And Formic Acid (Hcooh) In Pickle Process On Garut Sheep Leather Quality), Jajang Gumilar, Wendry Setiyadi Putranto, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan berbagai tingkat asam sulfat (H2So4) dan asam formiat (HCOOH) pada proses pikel  terhadap kualitas kulit jadi (leather) Domba Garut. Kualitas Kulit jadi didasarkan pada kadar keasaman (pH), kadar krom (Cr2O3), dan kadar air (H2O). Penelitian ini dilakukan secara eksperimen menggunakan pola faktorial dengan rancangan dasar rancangan acak lengkap, menggunakan 48 lembar kulit Domba Garut jantan ukuran 90. Kulit-kulit tersebut dibagi kedalam 16 kelompok. Tiap-tiap kelompok diberikan perlakuan penggunaan asam sulfat dan asam formiat. Masing-masing kelompok diulang sebanyak 3 kali ulangan. Hasil penelitian ini menunjukkan bahwa penggunaan berbagai tingkat asam sulfat (H2So4) dan asam …

Pengaruh Suplemen Zn-Proteinat, Cu-Proteinat Dan Kompleks Ca-Minyak Ikan Dalam Ransum Berbasis Pucuk Tebu Amoniasi Terhadap Performans Domba Jantan Persilangan Priangan X Barbados, U. Hidayat Tanuwiria, Budi Ayuningsih

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan mempelajari pengaruh suplemen Zn-Proteinat, Cu-Proteinat dan Ca-Minyak dalam ransum berbasis pucuk tebu diamoniasi terhadap pertumbuhan dan efisiensi ransum pada domba jantan persilangan Priangan x Barbados. Percobaan dilakukan terhadap 5 ekor domba jantan yang sedang tumbuh dengan menggunakan rancangan Bujur Sangkar Latin (BSL) 5x5,  lima macam ransum yaitu R1 = campuran 50% pucuk tebu amoniasi + 50% konsentrat, R2 = R1 + 2% Zn-proteinat, R3 = R1 + 2% Cu-proteinat, R4 = R2 + 2% Cu-proteinat, dan R5 = R4 + Ca-minyak ikan. Hasil yang diperoleh menunjukkan bahwa pemberian Zn-proteinat, Cu-proteinat dan komplek  Ca-minyak tidak mempengaruhi konsumsi ransum, …

Potensi Pakan Serat Dan Daya Dukungnya Terhadap Populasi Ternak Ruminansia Di Wilayah Kabupaten Garut, U. Hidayat Tanuwiria, A. Yulianti, A. Mushawwir

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mempelajari potensi pakan asal limbah tanaman pangan dan daya dukungnya terhadap populasi ternak ruminansia di wilayah kabupaten Garut. Penelitian dilakukan dengan cara survey dan pengumpulan data sekunder.  Hasil penelitian menunjukkan bahwa Potensi pakan asal rumput alam dan jerami asal limbah tanaman pangan di seluruh wilayah kabupaten Garut adalah 414.211,1 ton bahan kering (BK) yang terdiri atas 151.395,6 ton BK asal rumput dan 262.815,5 ton BK asal limbah tanaman pangan. Daya dukung pakan terhadap populasi ternak adalah 124.706 satuan ternak (ST), yang terdiri atas 45.580,5 ST ternak ruminansia kecil dan 79.125,5 ST ternak ruminansia besar.

Kata Kunci …

Potensi Pakan Asal Limbah Tanaman Pangan Dan Daya Dukungnya Terhadap Populasi Ternak Ruminansia Di Wilayah Sumedang (Agriculture By Product As Potential Feed And Its Carrying Capacity In Sumedang), U. Hidayat Tanuwiria, N. Mayasari, A. Yulianti

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mempelajari potensi pakan asal limbah tanaman pangan dan daya dukungnya terhadap populasi ternak ruminansia di wilayah kabupaten Sumedang. Penelitian dilakukan dengan cara survey dan pengumpulan data sekunder.  Hasil penelitian menunjukkan bahwa Potensi pakan asal rumput alam dan jerami asal limbah tanaman pangan di seluruh wilayah kabupaten Sumedang adalah 284.784,72 ton bahan kering (BK) yang terdiri atas 86.937,65 ton BK asal rumput dan 197.847,07 ton BK asal limbah tanaman pangan. Daya dukung pakan terhadap populasi ternak adalah 85.739,79 satuan ternak (ST), yang terdiri atas 26.174,21 ST ternak ruminansia kecil dan 59.565,58 ST ternak ruminansia besar. Potensi protein …

Pemanfaatan Gelatin Tepung Sagu (Metroxylon Sago) Sebagai Bahan Pakan Ternak Ruminansia (Utilization Of Sago (Metroxylon Sago) Gelatin As Feed Ruminant), Harry Triely Uhi

Jurnal Ilmu Ternak Universitas Padjadjaran

Telah dilakukan penelitian pemanfaatan gelatin sagu sebagai bahan pakan ternak ruminansia. Tujuan penelitian untuk mengetahui suhu, waktu dan ratio volume air yang tepat selama pemanasan sampai terbentuknya gelatinisasi. Justifikasi terjadinya proses gelatinisasi didasarkan atas rusaknya bentuk granula yang dilihat dengan  mikroskop elektron dan mikroskop polarisasi. Perlakuan yang digunakan R1 (Tepung sagu 100 gram + suhu   80 ⁰C); R2 (Tepung sagu 100 gram + suhu   85 ⁰C); R3 (Tepung sagu 100 gram + suhu   90 ⁰C); R4 (Tepung sagu 100 gram + suhu   95 ⁰C); dan R5 (Tepung sagu 100 gram + suhu 100 ⁰C). Parameter …

Pengaruh Tepung Cangkang Rajungan (Portunus Pelagicus) Sebagai Sumber Kitin Dalam Ransum Terhadap Kandungan Lemak Feses Dan Efisiensi Pakan Tikus Putih (Rattus Norvegiccus) Strain Wistar (The Effect Of Crabs Shells (Portunus Pelagicus) As A Source Of Chi, Muhamad Fatah Wiyatna, Irba U. Warsono, Aminuddin Parakkasi

Jurnal Ilmu Ternak Universitas Padjadjaran

Cangkang rajungan mengandung kitin yang persentasenya paling besar berupa polisakarida. Kitin dan derivat-derivatnya telah banyak digunakan dalam berbagai keperluan terutama untuk kesehatan. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh pemberian tepung rajungan sebagai sumber kitin terhadap efisiensi pakan dan kandungan lemak feces tikus putih Rattus norvegicus) strain Wistar. Penelitian menggunakan rancangan acak lengkap pola faktorial 2x5 dengan tiga kali ulangan. Faktor pertama adalah jenis kelamin dan kedua level tepung rajungan yaitu 0%, 5%, 10%, 15%, and 20%. Hasilnya penelitian menunjukkan tidak ada interaksi antara jenis kelamin dengan kandungan teung rajungan dalam ransum. Kandungan lemak dalam feces tertinggi diperoleh …

Pengaruh Lama Penyimpanan Semen Cair Ayam Buras Pada Suhu 5 0c Terhadap Periode Fertil Dan Fertilitas Sperma (The Storage Time Effect Of The Local Chicken Chilled Semen At 5 0c On Fertility And Fertile Period Of Sperm), Nurcholidah Solihati, Ruhijat Idi, Rangga Setiawan, I.Y. Asmara

Jurnal Ilmu Ternak Universitas Padjadjaran

Tujuan penelitian ini untuk mengetahui sejauh mana pengaruh lama penyimpanan semen cair ayam buras pada suhu 5⁰C terhadap periode fertil dan fertilitas sperma.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan setiap perlakuan diulang 6 kali.  Lama penyimpanan yang digunakan dalam penelitian ini adalah penyimpanan 1 jam (0 hari), 24 jam (1 hari) dan 48 jam (2 hari).  Peubah yang diukur adalah periode fertil dan fertilitas sperma. Data yang diperoleh dianalisis dengan analisis sidik ragam, dan untuk mengetahui perbedaan antar perlakuan diuji dengan menggunakan uji jarak berganda Duncan. Hasil penelitian menunjukan bahwa lama penyimpanan semen …

Host-Derived Interleukin-1Α Drives Tumor Immunosuppression By Reprogramming Tumor-Associated Myeloid Cells, Manikanda Raja Keerthi Raja, Gourab Gupta, Grace Atkinson, Katie Kathrein, Alissa R. Armstrong, R. Michael Gower, Igor Roninson Ph.D., Eugenia Broude, Mengqian Chen Ph.D., Hao Ji, Chang-Uk Lim, Hongjun Wang, Daping Fan, Peisheng Xu Ph.D., Hexin Chen

Faculty Publications

Myeloid cells are highly heterogeneous and play key roles in establishing an immunosuppressive tumor microenvironment (TME), but the mechanisms controlling their differentiation remain unclear. Using orthotopic murine breast cancer models, we investigated how interleukin-1α (IL-1α) influences this process. Deletion of host Il1α resulted in rejection of transplanted tumors. Single-cell RNA sequencing identified CX3CR1⁺ macrophages as the main source of IL-1α in the TME. Il1α deficiency disrupted monocyte–macrophage differentiation, reducing CX3CR1⁺ macrophages and increasing iNOS⁺ macrophages. Myeloid cells from Il1α⁻/⁻ hosts induced lower PD-1 and CTLA-4 expression in co-cultured CD8⁺ T cells than those from wild-type hosts. CellChat analysis showed …

Foxo1 Inhibition And Fadd Knockdown Have Opposing Effects On Anticancer Drug-Induced Cytotoxicity And P21 Expression In Osteosarcoma Cells, Danielle Walker, Antanay Hall, Alexis Bonwell, Nancy Gordon, Danielle Robinson, Mario G Hollomon

Faculty, Staff and Student Publications

Forkhead box class O1 (FOXO1) and fas-associated death domain (FADD) regulate cell death pathways and homeostatic processes such as cell cycle progression and apoptosis. FADD phosphorylation promotes nuclear localization of FOXO1, and FOXO1 regulates FADD expression. Therefore, it is plausible that FOXO1 and FADD have synergistic or antagonistic effects on cell cycle regulation and the response to anticancer drug treatment in cancer cells. In the present study, we report that AS1842856-mediated inhibition of FOXO1 reverses anticancer drug-induced cytotoxicity, while FADD knockdown increases anticancer drug-induced cytotoxicity in osteosarcoma (OS). Reversed anticancer drug-induced cytotoxicity was accompanied by G2/M cell cycle arrest and …

Premature Aging In Serious Mental Illness, Breno S Diniz, Gabriel R Fries, Chia-Ling Kuo, Ming Xu, Eric J Lenze

Faculty, Staff and Student Publications

Serious mental illnesses (SMIs), including major depressive disorder, bipolar disorder, and schizophrenia, have long been linked to cognitive decline, multiple chronic medical conditions, and premature mortality. These factors significantly contribute to the severe disability seen in SMIs, extending beyond the severity of psychopathology and indicating a premature aging phenotype associated with these conditions. The mechanisms that underlie the relationship between SMIs and the premature aging phenotype are not well understood, but recent evidence suggests that individuals with SMIs may exhibit accelerated biological aging. In this review, we present a comprehensive analysis of the current literature, demonstrating the potential association of …

Wavelet-Based Mechanomyographic Intensity Responses To Sustained Tasks Anchored To Low Versus High Ratings Of Perceived Exertion, Dolores G. Ortega, Robert W. Smith, Jocelyn E. Arnett, Tyler J. Neltner, Trevor D. Roberts, Justin S. Pioske, Richard J. Schmidt, Terry J. Housh

Journal for Sports Neuroscience

Purpose: This study examined the effects of sustained, isometric forearm flexion tasks anchored to ratings of perceived exertion of 2 (RPE2_FT) versus 8 (RPE8_FT) on the wavelet-based mechanomyographic (MMG) intensity responses from pretest and posttest maximal voluntary isometric contractions (MVICs) as well as the time course of changes in the MMG intensity responses throughout the sustained tasks. Methods: Eleven men (mean±SD: age=20.9±2.3 yrs) performed MVICs before and after the sustained tasks. The wavelet analyses decomposed the MMG signals onto a set of 11 wavelet bands (WBs). Repeated measures ANOVAs were used to analyze the MMG intensity …

The Ethical Limits Of Physician Autonomy In Reproductive Medicine, Isabel Martens

Honors Theses

In vitro fertilization is a relatively new medical practice that helps people who face infertility be able to reproduce. Reproductive medicine providers face ethical dilemmas often in their field, only some of which have standards of care. There are questions in the medical landscape pertaining to physician autonomy versus patient autonomy, which heavily apply to reproductive medicine providers. Patient autonomy is highly valued in medical ethics; however, physician autonomy should also be considered. When a physician decides who can and cannot reproduce, ethical considerations must be followed to maintain the practice of reproductive autonomy. Limited policies exist that manage reproductive …

Elevated Trna Halves In Olfactory Epithelial Cells Of Patients With Schizophrenia, Justin T. Gumas, Megumi Shigematsu, Karin E. Borgmann-Winter, C. G. Hahn, Yohei Kirino

Computational Medicine Center Faculty Papers

Schizophrenia patients' olfactory epithelial cells contain abundant immune-activating tRNA fragments, linking small RNA biology to inflammation and suggesting avenues for diagnostics.

Selective Deletion Of Slc2a1 From The Rpe Reveals That Rods But Not Cones Depend On Glucose Transport Across The Outer Blood-Retinal Barrier, Lauren L. Daniele, John Y.S. Han, Minzhong Yu, Ravi A. Sangani, Craig D. Beight, Cyrus Rostami, Philip D. Kiser, Neal S. Peachy, Nancy J. Philp

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

GLUT1 facilitates a continuous flow of glucose across the inner and outer blood-retinal barriers (BRBs) to support vision. To understand the extent to which photoreceptors rely on glucose transport across the outer BRB, we generated a tamoxifen-inducible conditional knockout of Slc2a1 in the retinal pigment epithelium (RPE) (RPE-iΔGlut1). In the RPE-iΔGlut1 mice, rod photoreceptors exhibited impaired outer segment renewal and decreased the expression of proteins involved in phototransduction and ciliary transport. Proteins regulating the retinal stress response increased. Cone photoreceptors were functional and viable 15 months post-tamoxifen treatment in the RPE-iΔGlut1 mice, while 70% of the rods died. …

Glutamatergic Projection Neurons In The Basal Forebrain Underlie Learned Olfactory Associational Valence Assignments, Pey-Shyuan Chin, Zhuokun Ding, Mikhail Kochukov, Snigdha Srivastava, Elizabeth H Moss, Qingchun Tong, Benjamin R Arenkiel

Faculty, Staff and Student Publications

Sensory perception is shaped by experience, giving stimuli behavioral significance. Basal forebrain (BF) cholinergic neurons in mice, which are crucial for arousal and motivation, also regulate sensory processing. Within BF nuclei, glutamatergic (vGlut2BF) neurons receive cholinergic input and modulate behaviors, but their roles in encoding sensory significance remain unclear. Using in vivo calcium imaging, we found that vGlut2BF neurons initially poorly encoded odor identity. However, their response to conditioned odors increased following associative learning, and their population activity more distinctly encoded paired stimuli, reflecting emergent value representation. Furthermore, pairing stimulation or inhibition of vGlut2BF neurons with specific odors altered odor …

Loss Of Payload Sensitivity And Other Mechanisms Of Resistance To T-Dxd In Her2-Mutant Nsclc: Implications For Subsequent Responsiveness To Her2 Tkis, Monique B Nilsson, Xiuning Le, Alissa Poteete, Xiaoxing Yu, Junqin He, Qian Huang, Yuji Shibata, Ximeng Liu, Cesar Moran, Ash A Alizadeh, Maximilian Diehn, Heather Wakelee, Diego Almanza, Scott Soltys, Takeshi Sugio, Jurik Mutter, Xiaoman Kang, Rui Wang, Soyeong Jun, Mohammad Shahrokh Esfahani, Hai Tran, Yuanxin Xi, Lingzhi Hong, Xiaofang Huo, Ashwani Kumar, Xiaoyang Ren, Kei Oguchi, Kazuhisa Minamiguchi, Caroline M Weipert, Jing Wang, Ralf Kittler, John V Heymach

Faculty, Staff and Student Publications

Introduction: Effective therapies are needed for patients with NSCLC with HER2-mutant tumors who progress on the HER2 antibody-drug conjugate trastuzumab deruxtecan (T-DXd), a standard-of-care treatment. A greater understanding of mechanisms mediating acquired T-DXd resistance and whether these tumors could benefit from HER2 tyrosine kinase inhibitors (TKIs) is needed.

Methods: Using preclinical models of acquired T-DXd resistance, LentiMutate scanning mutagenesis, and clinical analyses, we investigated mechanisms mediating acquired resistance to T-DXd and assessed the impact of each of these resistance mechanisms on cross-resistance to alternative HER2-targeting approaches.

Results: We determined that acquired resistance to T-DXd could occur through multiple mechanisms including …

Aberrant Cd25 And Increased Cd123 Expression Are Common In Acute Myeloid Leukemia With Kmt2a Partial Tandem Duplication And Are Associated With Flt3 Internal Tandem Duplication, Qing Wei, Guilin Tang, Shaoying Li, Sa A Wang, Pei Lin, Wei Wang, Sanam Loghavi, Wei J Wang, L Jeffrey Medeiros, Jie Xu

Faculty, Staff and Student Publications

Background: KMT2A partial tandem duplication (PTD) occurs in approximately 5-10% of acute myeloid leukemia (AML) cases and is associated with poor prognosis. While its cytogenetic and molecular features are well described, the immunophenotypic characteristics of AML with KMT2A-PTD remain incompletely defined.

Methods: We identified 47 cases of AML with KMT2A-PTD by optical genome mapping. All cases underwent flow cytometric immunophenotypic analysis and next-generation sequencing using an 81-gene panel.

Results: The cohort included 32 men and 15 women with a median age of 67 years (range, 19-87). Thirty-eight cases were de novo AML, and nine were secondary to myelodysplastic …

Emt-Induced Stem Cell And Mesenchymal Programs Can Be Decoupled Via Cell Division And Esrp1-Dependent Mechanisms, Petra Den Hollander, Maria Castaneda, Suhas V Vasaikar, Joanna Joyce Maddela, Claire Gould, Breanna R Demestichas, Robiya Joseph, Shivangi Agarwal, Abhijeet P Deshmukh, Alvina Zia, Shruti Shah, Tieling Zhou, Geraldine Raja, Paul Allegakoen, Nick A Kuburich, Mika Pietila, Chunxiao Fu, Jeffrey Chang, Chad J Creighton, William F Symmans, Rama Soundararajan, Sendurai A Mani

Faculty, Staff and Student Publications

Epithelial-to-mesenchymal transition (EMT) is known to induce both stemness and mesenchymal properties, and our findings reveal that these two programs can be uncoupled. During EMT, epithelial cells transition from symmetric divisions producing differentiated daughter cells to self-renewing daughter cells. When we block cell division and induce EMT, cells gain mesenchymal properties but not stemness, suggesting the importance of cell division for gaining stemness. We identified ESRP1 as a key regulator of EMT-driven stemness, which get downregulated during EMT in a cell division-dependent manner. Overexpression of ESRP1 prevents the gain of stemness without affecting the mesenchymal program. Only the stemness and …

Role Of The Etv5/P38 Signaling Axis In Aggressive Thyroid Cancer Cells, Jerry H Houl, Rozita Bagheri-Yarmand, Muthusamy Kunnimalaiyaan, Paola Miranda Mendez, Joseph L Kidd, Ali Dadbin, Andrea Ruiz-Jurado, Parag A Parekh, Ying C Henderson, Nikhil S Chari, Aatish Thennavan, Reid T Powell, Clifford C Stephan, Xiao Zhao, Anastasios Maniakas, Roza Nurieva, Naifa L Busaidy, Maria E Cabanillas, Ramona Dadu, Mark Zafereo, Jennifer R Wang, Stephen Y Lai, Marie-Claude Hofmann

Faculty, Staff and Student Publications

Patients with poorly differentiated thyroid cancer (PDTC) and anaplastic thyroid cancer (ATC) face a much poorer prognosis than those with differentiated thyroid cancers. Around 25% of PDTCs and 35% of ATCs carry the BRAFV600E mutation, which constitutively activates the MAPK pathway, a key driver of cell growth. Although combining BRAF and MEK inhibitors can shrink tumors, resistance often develops. The exact cause of this resistance remains unclear. We previously found that in PDTC and ATC cells, the BRAFV600E mutation is strongly linked to the expression of ETV5, a transcription factor downstream of the MAPK pathway. In the current study, we …

Advances In Targeting Her2 Across Cancer Subtypes: A Pan-Tumor Approach, Taiwo Adesoye, Ecaterina E Dumbrava, Kanwal P S Raghav, Aysegul A Sahin, Hui Chen, Sunyoung S Lee, Milind M Javle, Shubham Pant, Omar Alhalabi, Xiuning Le, Vicente Valero, Paula R Pohlmann, Funda Meric-Bernstam

Faculty, Staff and Student Publications

Human epidermal growth factor receptor 2 (HER2) is an established therapeutic target in multiple solid tumors, particularly breast and gastric cancers. Significant advancements have been made in the development of HER2-targeted therapies, including monoclonal antibodies, tyrosine kinase inhibitors, antibody-drug conjugates (ADC), and novel bispecific antibodies. These agents have revolutionized the treatment landscape for HER2-positive metastatic cancers, resulting in improved progression-free and overall survival, and quality of life for patients. Beyond breast and gastric cancers, HER2 expression/amplification has been observed in other solid tumors, such as colorectal, lung, bladder, ovarian, and biliary tract cancers, offering new opportunities for personalized therapy in …

Integrative Spatial Multi-Omics Reveal Niche-Specific Inflammatory Signaling And Differentiation Hierarchies In Aml, Enes Dasdemir, Ivo Veletic, Christopher P Ly, Andres E Quesada, Christopher D Pacheco, Fatima Z Jelloul, Pamella Borges, Sreyashi Basu, Sonali Jindal, Zhiqiang Wang, Alexander Lazar, Khalida M Wani, Dinler A Antunes, Patrick K Reville, Preethi H Gunaratne, Robert J Tower, Padmanee Sharma, Hussein A Abbas

Faculty, Staff and Student Publications

Acute myeloid leukemia (AML) is a clonal disorder characterized by immature blasts and arrested differentiation that primarily affects the bone marrow (BM) and occasionally presents as extramedullary (EM) disease. EM manifestations highlight AML's adaptability to distinct microenvironments, which we examined using spatial analyses of medullary and EM tissues. We describe a workflow for Visium-based spatial transcriptomics in medullary and EM AML, revealing insights into cell-cell communication and the spatial organization of AML hierarchies. In BM, monocytes and granulocyte-monocyte progenitors colocalized with leukemic populations, sharing molecular signatures with those in EM sample. CXCL12-CXCR4-mediated communication correlated with PI3K/AKT/mTOR signaling in inflammatory niches.

Brain Gains: A Review Of Remediation Treatment Strategies Using Exercise Following Traumatic Brain Injury, Jessica Smith, Kristin Biggins, Loriann Helgeson

The Open Journal of Occupational Therapy

In this Opinions in the Profession article, we explore remediation treatment strategies using exercise for recovery from post-traumatic brain injury and aim to justify the use of such treatment in the occupational therapy profession to increase skills for functional gain. Common treatment strategies used by occupational therapists after brain injury are compensatory strategies, indicating the client learns a new way to perform the task. Though effective, it is also necessary to consider how remediation and neuroplasticity are essential. Teaching new approaches to complete tasks results in functional gains; however, focusing on foundational skills using exercises to relearn motor patterns to …

Epigenome-Wide Association Study Meta-Analysis Of Bmi In African Americans, Kendra Ferrier, Mariaelisa Graff, Iain R Konigsberg, Maggie Stanislawski, Heather M Highland, Laura M Raffield, April P Carson, Eric Boerwinkle, Jill M Norris, Chris R Gignoux, Audrey E Hendricks, Sridharan Raghavan, Kari E North, Kristin L Young, Anne E Justice, Matthew A Allison, Mathew J Budoff, Silva Kasela, François Aguet, Joshua J Joseph, Charles Kooperberg, Stephen S Rich, Jerome I Rotter, Ethan M Lange, Leslie A Lange

Faculty, Staff and Student Publications

Despite considerable advances in identifying risk factors for obesity, gaps remain in our understanding about its etiology. Genetic variants explain only a small portion of variation in obesity-related traits such as body mass index (BMI). Epigenetic regulation, which controls gene expression and is influenced by environmental and genetic factors, may account for additional variability in BMI. Epigenetic studies of BMI have largely been conducted in European ancestry populations, despite the disproportionate burden of obesity in African Americans (AAs). We conducted a sex-stratified BMI epigenome-wide association study meta-analysis in AA participants from the Jackson Heart Study (n = 1,604) and …