Medicine and Health Sciences Commons^™

Efficacy Of X-Ray Phytosanitary Irradiation On The Infectivity And Reproduction Of Angiostrongylus Cantonensis In Experimentally Infected Rats, Susan I. Jarvi, Lisa M. Kaluna, Steven C. Hess, Lindsey Hamilton, Carmen Antaky, Robert T. Sugihara, Israel L. Leinbach, Yaeko Tagami, Argon Steel, Kathleen Howe, Steven Jacquier, Forrest Cookman, Jocelyn Diaz, John Jacob, Peter Follett

United States Department of Agriculture Wildlife Services: Staff Publications

Angiostrongylus cantonensis is a globally distributed nematode and the leading cause of eosinophilic meningitis in humans. As a global hotspot for this disease, Hawaii’s agricultural exports may be contributing to the spread of A. cantonensis. Phytosanitary irradiation doses of 150 or 400 Gy provide quarantine security against multiple insect pests. We evaluated the in vitro and in vivo effects of phytosanitary irradiation on infectious, third-stage A. cantonensis juveniles. In vitro experiments directly exposed juveniles to irradiation doses ranging from 200 to 1,000 Gy. Results showed low mortality and no dose response across all treatments 27 days post-irradiation. …

Impact Of Oxytocin And Tetrahydrocannabinol On Signs Of Fear In The Rat, Jair E. Alvarez Jr.

EWU Masters Thesis Collection

BACKGROUND: Oxytocin (OXT) has been used as a post-treatment for post-traumatic stress disorder (PTSD). Previous research suggests that pre-treatment of intranasal OXT attenuates signs of fear in rats; however, the impact of intraperitoneal (i.p) OXT pre-treatment on signs of fear in rats is unknown. We also explored the effects of delta-9 (psychoactive component of cannabidiol) and delta-8 (delta-9 isomer) THC pre-treatment on attenuating signs of fear in the rat. METHODS: For each experiment, male Sprague-Dawley rats were divided into 5 groups, (n=8 per group); 1.) controls (vehicle and no foot shock), 2.) shock (vehicle and foot shock), 3.) low dose …

Disability Reduction Following A Lumbar Stabilization Exercise Program For Low Back Pain: Large Vs. Small Improvement Subgroup Analyses Of Physical And Psychological Variables, Christian Larivière, Richard Preuss, Marie-France Coutu, Michael J. Sullivan, Nicolas Roy, Sharon M. Henry

Études primaires

Background: Little is known about why patients with low back pain (LBP) respond differently to treatment, and more specifically, to a lumbar stabilization exercise program. As a first step toward answering this question, the present study evaluates how subgroups of patients who demonstrate large and small clinical improvements differ in terms of physical and psychological changes during treatment. Methods: Participants (n = 110) performed the exercise program (clinical sessions and home exercises) over eight weeks, with 100 retained at six-month follow-up. Physical measures (lumbar segmental instability, motor control impairments, range of motion, trunk muscle endurance and physical performance tests) were …

Effect Of Personalized Spinal Profile On Its Biomechanical Response In An Emg-Assisted Optimization Musculoskeletal Model Of The Trunk, C. Larivière, A.H. Eskandari, H. M., F. Ghezelbash, D. Gagnon, A. Shirazi-Adl

Études primaires

Recent developments in musculoskeletal (MS) modeling have been geared towards model customization. Personalization of the spine profile could affect estimates of spinal loading and stability, particularly in the upright standing posture where large inter-subject variations in the lumbar lordosis have been reported. This study investigates the biomechanical consequences of changes in the spinal profile. In 31 participants (healthy and with back pain), (1) the spine external profile was measured, (2) submaximal contractions were recorded in a dynamometer to calibrate the EMG-driven MS model and finally (3) static lifting in the upright standing challenging spine stability while altering load position and …

Innate And Adaptive Immune System Consequences Of Post-Traumatic Stress Disorder, Tatlock H. Lauten, Tamara Natour, Adam J. Case

Journal Articles: Cellular & Integrative Physiology

In the field of psychiatry, biological markers are rarely, if ever, used in the diagnosis of mental health disorders. Clinicians rely primarily on patient histories and behavioral symptoms to identify specific psychopathologies, which makes diagnosis highly subjective. Moreover, therapies for mental health disorders are aimed specifically at attenuating behavioral manifestations, which overlooks the pathophysiological indices of the disease. This is highly evident in posttraumatic stress disorder (PTSD) where inflammation and immune system perturbations are becoming increasingly described. Further, patients with PTSD possess significantly elevated risks of developing comorbid inflammatory diseases such as autoimmune and cardiovascular diseases, which are likely linked …

Chemoreflex Sensitization Occurs In Both Male And Female Rats During Recovery From Acute Lung Injury, Kajal Kamra, Irving H. Zucker, Harold D. Schultz, Han-Jun Wang

Journal Articles: Cellular & Integrative Physiology

Introduction: Sex-specific patterns in respiratory conditions, such as asthma, COPD, cystic fibrosis, obstructive sleep apnea, and idiopathic pulmonary fibrosis, have been previously documented. Animal models of acute lung injury (ALI) have offered insights into sex differences, with male mice exhibiting distinct lung edema and vascular leakage compared to female mice. Our lab has provided evidence that the chemoreflex is sensitized in male rats during the recovery from bleomycin-induced ALI, but whether sex-based chemoreflex changes occur post-ALI is not known. To bridge this gap, the current study employed the bleomycin-induced ALI animal model to investigate sex-based differences in chemoreflex activation during …

Modifying A Health Education Program By Utilizing Methods From Community-Based Participatory Research Framework, Tashara Grandys

Graduate Research Theses & Dissertations

Purpose: This study determined the use of Community Based Participatory Research (CBPR) and guided the modification of the Whole Body Approach (WBA) health education program. The modified program was pilot tested and implemented among neighbors at Northern Illinois Food Bank. The impact of the modification was assessed via pre and post surveys. Background and significance: WBA is a 10-week program developed by faculty at Northern Illinois University and has been taught by graduate students in collaboration with the Northern Illinois Food Bank since 2016. The program is based on the Satter Eating Competence Model and Health at Every Size and …

Student Attitudes Towards Their Program Area Of Study In A College Of Health And Human Services, Melanie Regan

Graduate Research Theses & Dissertations

Poor retention rates can be an indicator of systematic problems within a university’s culture of academic affairs and administration. Improving student retention rates requires an understanding of why students are not returning to campus. This research aimed to develop and implement a survey analyzing students' sensory comfort, relevance, reaction to their area of study, sense of belonging, and self-efficacy within a College of Health and Human Sciences. A descriptive research design was utilized for this study to obtain information on the study population to survey participants' overall attitudes towards their experience in a program of study in a college of …

Role Of Human Intermediate Form Prl Receptor I-Tail In The Pathogenesis Of Breast Cancer, Shanwei Shen

Theses and Dissertations

Breast cancer continues to be the second most common cancer in women in the United States. The neuroendocrine hormone human prolactin (hPRL) plays an important role in normal mammary gland development and growth, stimulating normal breast tissue proliferation and differentiation. Considerable evidence has shown that hPRL is involved in the malignant transformation of human breast tissue. hPRL enhances viability, invasiveness, and proliferation of breast cancer cells in vitro. Evidence from epidemiologic and genetic studies also implicates role of hPRL in the pathogenesis of breast cancer. Its cognate receptor the human prolactin receptor (hPRLr) is required for the action of hPRL. …

The Development And Evaluation Of A Method For Complete Collagenase I Digestion Of Bos Taurus Bone Samples, Reilly Price

Master of Science in Forensic Science Directed Research Projects

Current methods for processing aged forensic bone samples for DNA analysis involve pulverization and organic extraction, which further destroys already degraded DNA and uses large amounts of limited samples. Additionally, the success rates for this type of analysis vary widely as homogenization incorporates PCR inhibitors into the sample. Little research has explored how pulverization results in DNA damage in profiles obtained from skeletal remains. This study proposes an enzymatic digestion method to separate osteocytes from PCR inhibitors in the extracellular bone matrix to improve the quality and quantity of DNA isolated from aged bone samples. The technique described here used …

Opioid System's Potential Involvement In Ketamine's Cognitive And Antidepressant-Like Effects In Rats, Fan Zhang

Theses and Dissertations

Depression stands as one of the most pervasive mental health challenges worldwide, including the United States. Conventional antidepressants typically display a delayed onset of therapeutic action (4-8 weeks) and lack efficacy in a substantial portion of patients (over 40% of depressed patients are treatment-resistant). The dissociative anesthetic ketamine, which is a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, has emerged as a promising novel treatment due to its rapid and prolonged antidepressant effects, and it is especially beneficial for treatment-resistant depression. Nevertheless, the precise neurochemical mechanisms underlying ketamine’s antidepressant properties remain elusive, hampering the development of safer and more efficacious treatments. The present …

Exploring Usability In Exercise Interventions: From Conceptualization To Measurement And Application, Anne Inger Mørtvedt

Dissertations, Master's Theses and Master's Reports

Exercise interventions hold promise for preventing and treating numerous conditions, diseases, and injuries. However, these interventions will only be effective if they are being used. Unfortunately, uptake and adherence to prescribed exercise and physical activity guidelines are insufficient. Some reasons for this include lack of knowledge, resources, flexibility, and enjoyment. Exercise program developers need to not only consider the effectiveness of the program during the development phase, but also involve end-users and receive feedback on program usability to determine likelihood of uptake and adoption. Usability testing can be used to detect barriers to use and implementation likelihood but has not …

The Effects Of Resistance Training On Older Adults: Increasing Healthspan And Lowering Disability Rates, Michael M. Samuel

Honors Undergraduate Theses

This study analyzed the research on older adults and resistance training exercise and assessed effects on health span and lowering disability rates. A targeted search of the literature was employed using the following key words: sarcopenia, resistance training, hypertrophy, quality of life, disability, activities of daily living, strength training, falls, and functional strength. These were used to find articles that were relevant to the research. Articles were excluded if they focused on young athletes as they did not meet my research age group. Articles that focused on nutrition were also excluded as they pulled the focus away from the impact …

Investigating The Role Of Glycemic Control In Cognitive Impairement Risk Among Elderly Individuals With Type 2 Diabetes: A Scoping Review, Julia Pessaia

Honors Undergraduate Theses

Type 2 diabetes mellitus (T2DM), presents a significant health burden globally, affecting millions of individuals, especially in the elderly population. While its association with cardiovascular diseases and cognitive impairments is well-documented, further research on the precise influence of glucose control on cognitive outcomes in elderly T2DM patients is necessary. This scoping review aims to address this gap by investigating the impact of HbA1c levels representing glycemic control on the risk of developing cognitive impairments in elderly patients with T2DM. A literature search was conducted on MEDLINE and eligible studies involved T2DM patients aged 60 or older, with documented cognitive function …

Hybridization Kinetics Of Four-Way Junctions Localized On A Dna Scaffold, Katherine N. Taylor

Honors Undergraduate Theses

DNA computing is an ever-growing field with scientists trying to design structures that optimize logic gate communication to develop fast, biologically compatible, computational structures. We hypothesize that by using the principles of DNA computing, it is possible to design a DNA tile capable of studying localized DNA hybridization that can differentiate between oligonucleotides of different structural conformations. This includes synthetically manipulating DNA into a nanostructure that can perform Boolean logic functions to calculate the different rates of hybridization. To test our hypothesis, we designed a DNA Tile that incorporated a 4WJ using YES logic. Linear and hairpin single-stranded (ss) DNA …

A Tool To Automate Neuropathological Assessment In Huntington Disease Mouse Models, Samuel A. Moldenhauer

Honors Undergraduate Theses

As our life expectancy continues to rise, so does the prevalence of neurodegenerative diseases, such as Huntington disease (HD). Neurodegeneration leads to progressive regional brain atrophy, typically initiating prior to symptom onset. With the advancements of medical treatments designed to target neurodegeneration, researchers measure their impact on atrophy in animal models to assess their effectiveness. This is important because treatments designed to combat neuropathology are more likely to modify the disease itself, per contra to treatments designed to mask or treat symptoms. One method of brain region size quantification is magnetic resonance imaging (MRI), which while accurate, is prohibitively expensive. …

Genetic And Biochemical Analysis Of Novel Borrelia Burgdorferi Genes Bb0244 And Bb0246, Christian J. Ruiz-Rodriguez

Honors Undergraduate Theses

As the cause of the most common vector-borne disease in the US, Borrelia burgdorferi continues to affect an estimated hundreds of thousands of patients each year. The spirochete boasts one of the most complex genomes in comparison to all other prokaryotes as it is fragmented across up to 21 different replicons. Moreover, the functions of a majority of the encoded genes remain unknown. Gene of unknown function, bb0244, along with putative co-transcribed genes bb0245, and bb0246 are hypothesized to be involved in the vital functions of cell division and cell wall biogenesis. B. burgdorferi lacking bb0244 demonstrate a significant growth …

Epitope Mapping Novel Monoclonal Antibodies Against Borrelia Burgdorferi Surface Proteins Ospc And Bmpa, Eliza M. Doyle

Honors Undergraduate Theses

In response to the progressive nature of Lyme disease, direct diagnostic testing of Borrelia burgdorferi surface proteins was proposed as a quicker and more sensitive alternative to the current indirect detection methods. To determine the ability of novel monoclonal antibodies to detect the surface lipoproteins BmpA and OspC, recombinant fragments of these proteins were created. These fragments were tested against the putative diagnostic antibodies via western blotting as a means to map the epitope of each of the proteins that the respective antibodies recognize. All BmpA fragments with deletions on the N-terminal end exhibited reactivity, indicating that the epitope is …

Co-Occurrence Of Autism Spectrum Disorder And Attention-Deficit/Hyperactivity Disorder With Idiopathic Hypersomnia: Data From The Hypersomnia Foundation Registry, Sophie Sevan Mikkelsen

Honors Undergraduate Theses

In recent years, hypersomnia patients and researchers alike have increasingly discussed the possibility of heightened comorbidities, or co-occurrences, of autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) with the central disorders of hypersomnolence: idiopathic hypersomnia (IH) and narcolepsy (N). While a few scarce studies have investigated a link between ADHD and N, there has been no research on the co-occurrence of these conditions with IH. This study, using data from the Hypersomnia Foundation’s CoRDS Patient Registry, is the first to identify self-reported rates of ASD and ADHD diagnoses by people with idiopathic hypersomnia. Four chi-square tests were conducted to determine …

Dysbiosis In Parkinson's Disease: A Meta-Analysis Of Gut Microbiota And Its Role In Gastrointestinal Dysfunction And Disease Progression, Ayan M. Siddiqui Mr.

Honors Undergraduate Theses

Parkinson's Disease (PD) is a neurodegenerative disorder characterized by motor and nonmotor symptoms, including gastrointestinal (GI) dysfunction. Symptoms such as constipation, bloating, and nausea often show early signs before the onset of motor symptoms. Recent research has suggested an essential role of the human gut microbiome and gut dysbiosis, an imbalance in gut microbiota composition, in the pathogenesis of PD. This thesis aims to assess the potential relationship between gut dysbiosis and PD by conducting a meta-analysis, focusing on differences in gut microbiota between PD patients and healthy controls and evaluating their implications for GI dysfunction and PD progression. The …

Evaluation Of Common Complications Following Tetralogy Of Fallot Surgical Repair By Magnetic Resonance Imaging, Zainab Ahmad Ramadan, Mohamed Salah Ayyad

Mansoura Medical Journal

Background: The operative procedures for correction of tetralogy of Fallot (TOF) concentrates on the impact of pulmonary valve regurgitation (PR) on the function of right ventricle (RV) pointing to alleviating right ventricle outflow tract (RVOT) narrowing, and minimizing the PR degree at the same time, closing ventricular septal defect (VSD). Various complications could be encountered after TOF repair as; PR, dilated RV with/without impaired function, RVOT residual obstruction/ dilatation, residual VSD, tricuspid regurge (TR), aortic root dilatation with/without regurge and significant difference in differential pulmonary flow. Objective: Address the most common complications that could be encountered following TOF surgical repair, …

Association Between Serum Procalcitonin Level With Exacerbation Severity In Chronic Obstructive Pulmonary Disease Patients, Dalia Abdellateif Abdelghany, Shimaa R. Hendawy, Rania Mostafa, Heba Wagih Abdelwahab

Mansoura Medical Journal

Background: There is a significant impact on morbidity, mortality, and quality of life due to chronic obstructive pulmonary disease exacerbations. Individuals with acute exacerbation of chronic obstructive pulmonary disease had considerably higher mean serum procalcitonin levels (PCT) compared to individuals with stable chronic obstructive pulmonary disease, according to previous investigations. Objective: This study aimed to assess whether procalcitonin levels were associated with the severity of exacerbations in patients with chronic obstructive pulmonary disease. Methods: thirty-nine patients who were suffering from an acute exacerbation of chronic obstructive pulmonary disease were included. Included patients were classified as having either no respiratory failure, …

Study Of Serum Bilirubin In Relation To Stable Chronic Coronary Artery Disease Severity And Outcome After Percutaneous Intervention, Khaled E Mahfouz, Ahmed W Suliman, Mahmoud A Youssef, Adel M Osman

Mansoura Medical Journal

Background: Bilirubin is endogenous antioxidant and increased its physiological concentrations reduce severity of coronary artery disease with better prognosis after PCI. Objective: Assess serum bilirubin level in patients with stable chronic coronary artery disease and correlate it with the severity of this disease and outcome after PCI. Methods: Cross sectional prospective observational study included 203 Patient with stable chronic coronary artery disease divided into 2 groups according to bilirubin: group I ≤ 0.6mg/dl group II > 0.6 (up to 1.2) mg/dl 100 cases underwent elective coronary angiography and PCI with full clinical examination, laboratory tests: total serum bilirubin, creatinine, lipid profile, …

Evaluation Of Low Dose Intrathecal Bupivacaine With Dexmedetomidine In Geriatric Patients Undergoing Traumatic Hip Surgery, Rania E. Elbadrawy, Sherine A Bakrey, Reem Abdelraouf Elsharkawy

Mansoura Medical Journal

Abstract: Background: Spinal anesthesia (SA) is typically utilized during surgical treatment of severe hip fractures. Searching for early ambulation and reduction of frequency and severity of hypotension, and after adding dexmedetomidine (DEX), different intrathecal (IT) dosages of 0.5% hyperbaric bupivacaine had been used to determine the lowest dosage that will result in a good block without hemodynamic instability. Methods: At random, 78 patients were divided into three groups. A 7.5 mg of 0.5% hyperbaric bupivacaine was administered in the first group10 mg in the second and 12.5 mg in the third group. Dexmedetomidine (5 μg) was added to each group. …

The Effect Of Ultrasound-Guided Thoracic Retrolaminar Block Versus Erector Spinae Plane Block On Opioid Consumption After Pediatric Open-Heart Surgery: A Randomized Controlled Non-Inferiority Study, Ahmed Abdellatif Elsayed Abdelwahab, Ghada Fathy Elrahmawy, Ibrahim Ibrahim Abdelbaser, Rania Elmohamady Elbadrawy

Mansoura Medical Journal

Background Pain following open cardiac surgery in pediatric patient is crucial for the anesthesiologist Objective to compare a single-shot US-guided bilateral thoracic retrolaminar block (TRLB) and erector spinae plane block (ESPB) as regarding 24 hours postoperative opioid analgesic requirements as primary outcome, while time to first rescue analgesic, the post-operative pain score and rate of block-related complications were secondary outcomes Methods Each study group included 50 patients. TRLB group received 0.4 ml/kg bupivacaine (BPV) 0.25% injected under US guidance bilaterally as the needle touched the lamina of the T4 vertebra; while ESPB group received 0.4 ml/kg BPV 0.25% injected under …

Testing The Strength Of Thin Feather-Edge Monolithic Multilayered Zirconia Crowns: A Pilot Study With A Novel Acoustic Test, Estelle Saab, Camille Haddad, Maher Abboud, Maha Daou, Amine Zoghbi

International Arab Journal of Dentistry

Introduction: Limited research has explored the mechanical characteristics of recently introduced multilayered monolithic zirconia (MZC) crowns with feather-edge margins design. Moreover, most of the conventional techniques for evaluating the mechanical behavior of brittle dental ceramics rely on destructive tests, making it impossible to precisely evaluate real strength values due to premature crack initiation detection failure.

Objectives: The main objective of the study was to assess the load capacity of translucent multilayered monolithic zirconia crowns with feather-edge margin thicknesses of 0.4 mm and 0.5 mm. Additionally, the research introduced a novel non-destructive acoustic emission testing (AET) technique in the …

Navigating Asynchronous Online Learning: The Impacts Of Active Learning And Assigned Group Work On Student Motivation, Belonging, And Inclusion, Shea Elisabeth Carr

Theses and Dissertations--Biology

Motivation, belonging, and inclusion are all critical factors for student success but can be difficult to support in asynchronous online courses. Active learning and required group work have been shown to increase student motivation in in-person and synchronous online courses, but little is known about their impact in online asynchronous settings. This dissertation aimed to 1) evaluate the effect of active learning on student motivation in an online asynchronous setting and 2) evaluate the impact of assigned group work on student motivation and perceptions of belonging and inclusion within the context of an online, asynchronous physiology laboratory course. It was …

Cd8+ T Cell Metabolic Flexibility Elicited By Cd28-Ars2 Axis- Driven Alternative Splicing Of Pkm Supports Antitumor Immunity, G. Aaron Holling, Colin A. Chavel, Anand P. Sharda, Mackenzie M. Lieberman, Caitlin M. James, Shivana M. Lightman, Jason H. Tong, Guanxi Qiao, Tiffany R. Emmons, Thejaswini Giridharan, Shengqi Hou, Andrew M. Intlekofer, Richard M. Higashi, Teresa W. M. Fan, Andrew N. Lane, Kevin H. Eng, Brahm H. Segal, Elizabeth A. Repasky, Kevin P. Lee, Scott H. Olejniczak

Markey Cancer Center Faculty Publications

Metabolic flexibility has emerged as a critical determinant of CD8+ T-cell antitumor activity, yet the mechanisms driving the metabolic flexibility of T cells have not been determined. In this study, we investigated the influence of the nuclear cap-binding complex (CBC) adaptor protein ARS2 on mature T cells. In doing so, we discovered a novel signaling axis that endows activated CD8+ T cells with flexibility of glucose catabolism. ARS2 upregulation driven by CD28 signaling reinforced splicing factor recruitment to pre-mRNAs and affected approximately one-third of T-cell activation-induced alternative splicing events. Among these effects, the CD28-ARS2 axis suppressed the expression of the …

The Carboxy Terminus Causes Interfacial Assembly Of Oleate Hydratase On A Membrane Bilayer, Christopher D. Radka, Christy R. Grace, Hale S. Hasdemir, Yupeng Li, Carlos C. Rodriguez, Patrick Rodrigues, Michael L. Oldham, M. Zuhaib Qayyum, Aaron Pitre, William J. Maccain, Ravi C. Kalathur, Emad Tajkhorshid, Charles O. Rock

Markey Cancer Center Faculty Publications

The soluble flavoprotein oleate hydratase (OhyA) hydrates the 9-cis double bond of unsaturated fatty acids. OhyA sub- strates are embedded in membrane bilayers; OhyA must remove the fatty acid from the bilayer and enclose it in the active site. Here, we show that the positively charged helix- turn-helix motif in the carboxy terminus (CTD) is responsible for interacting with the negatively charged phosphatidylgly- cerol (PG) bilayer. Super-resolution microscopy of Staphylo- coccus aureus cells expressing green fluorescent protein fused to OhyA or the CTD sequence shows subcellular localization along the cellular boundary, indicating OhyA is membrane- associated and the CTD sequence …

Cognitive Decline, Aβ Pathology, And Blood–Brain Barrier Function In Aged 5xfad Mice, Geetika Nehra, Sasivimon Promsan, Ruedeemars Yubolphan, Wijitra Chumboatong, Pornpun Vivithanaporn, Bryan J. Maloney, Anusorn Lungkaphin, Bjoern Bauer, Anika M. S. Hartz

Markey Cancer Center Faculty Publications

Background

Patients with Alzheimer’s disease (AD) develop blood–brain barrier dysfunction to varying degrees. How aging impacts Aβ pathology, blood–brain barrier function, and cognitive decline in AD remains largely unknown. In this study, we used 5xFAD mice to investigate changes in Aβ levels, barrier function, and cognitive decline over time.

Methods

5xFAD and wild-type (WT) mice were aged between 9.5 and 15.5 months and tested for spatial learning and reference memory with the Morris Water Maze (MWM). After behavior testing, mice were implanted with acute cranial windows and intravenously injected with fluorescent-labeled dextrans to assess their in vivo distribution in the …