Medicine and Health Sciences Commons^™

A Geometric Modeling Of Dog Intestine, Hüseyi̇n Yildiz, Kadri̇ Arslan, İhsani̇ye Coşkun, Bahri̇ Yildiz

Turkish Journal of Veterinary & Animal Sciences

In computer-assisted surgical applications, reconstruction of 3-D morphological peculiarities are required. Herein, we present a geometric modeling of the separate intestinal sections of the canine. The intestine of the dog was considered a tubular shape along a special curve. Seven male Turkish shepherd dogs were used for the modeling study. The length (cm) and diameter (mm) of the intestines were measured with digital calipers and formulated. These models were then compared to their original photographs. It was concluded that the geometric modeling and experimental work were consistent. These kinds of organ modeling techniques will enable medical lecturers to show 3-D …

The Growth And Survival Characteristics Of Lambs Produced By Commercial Crossbreeding Kıvırcık Ewes With F_2 Rams With The German Black-Headed Mutton Genotype, Bülent Eki̇z, Ahmet Altinel

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to compare the growth and survival characteristics of crossbred lambs produced by mating Kıvırcık ewes with German Black-Headed Mutton (GBM) x Kıvırcık (F_2) rams, GBM x Merino (F_2) rams, as well as the growth and survival characteristics of purebred Kıvırcık lambs. The data were obtained from 59 (GBM x Kıvırcık) x Kıvırcık, 59 (GBM x Merino) x Kıvırcık, and 60 purebred Kıvırcık lambs. The birth weights of (GBM x Kıvırcık) x Kıvırcık, (GBM x Merino) x Kıvırcık, and purebred Kıvırcık lambs were 4.08, 4.32, and 3.85 kg, respectively. The differences of birth weight between the genotype …

In Vitro Effects Of Some Antibiotics On Glutathione Reductase Obtained From Chicken Liver, Hülya Demi̇r, Mustafa Erat, Hali̇s Şaki̇roğlu

Turkish Journal of Veterinary & Animal Sciences

Glutathione reductase (GR) is important in maintaining high levels of reduced glutathione in cells. The enzyme is inhibited by some drugs used in antimicrobial chemotherapy. The effects of some antibiotics on GR activity were investigated in this study. At first, the enzyme, having a specific activity of 88.28 EU/mg proteins, was purified 1636-fold with a yield of 35%. Purity of the enzyme was checked by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Activity of the enzyme was determined spectrophotometrically at 340 nm and in vitro effects of ofloxacin, levofloxacin, cefazolin, and cefepime were determined. The antibiotics used in …

Treatment Of Infertile Cows: Efficacy Of Pgf_{2\Alpha} And Gentamicin Or Their Combination In A Prid Regime, Yavuz Öztürkler, Ömer Uçar

Turkish Journal of Veterinary & Animal Sciences

The efficacy of PGF_{2\alpha} and intrauterine gentamicin or their combination in a progesterone releasing intravaginal device (PRID) regime was investigated for treatment of infertility in cows. From 46 infertile cows, body condition scores (BCSs) were recorded and the cows were then divided into 4 experimental groups according to the synchronisation protocol and intrauterine gentamicin administration as follows: i) PRID (1.55 g of progesterone and 10 mg of oestradiol benzoate) for 12 days plus artificial insemination (AI) at 48 and 72 h (group 1, n = 8), ii) PRID for 12 days plus an i.m. injection of 0.150 mg of cloprostenol …

Effect Of Energy Deficiency During Late Pregnancy In Chios Ewes On Free Fatty Acids, \Beta-Hydroxybutyrate And Urea Metabolites, Mehmet Hani̇fi̇ Durak, Ayşen Altiner

Turkish Journal of Veterinary & Animal Sciences

Pregnant Chios ewes with high twin-lambing rates fed a low energy ration during the final periods of pregnancy were studied. The aim of this study was to determine whether pregnancy toxemia occurred in these ewes by monitoring the changes in the concentrations of serum free fatty acids (FFAs), plasma \beta -hydroxybutyrate (\beta -HB) and urea. Thirty-eight Chios ewes were used. The animals were divided into 3 groups: pregnant normal energy (PNE), pregnant low energy (PLE), and non-pregnant normal energy (N-PNE). On day 105 of gestation, the animals were begun to be fed with the treatment rations. The feeding regime was …

Pathological And Immunohistochemical Investigation Of Naturally Occurring Systemic Candida Albicans Infection In Dogs, Recai̇ Tunca, Tolga Güvenç, Rifki Haziroğlu, Lale Ataseven, Hasan Özen, Ni̇hat Toplu

Turkish Journal of Veterinary & Animal Sciences

Systemic candidiasis is a rare disease of animals. In this study, the clinical course, and cultural and pathological findings of systemic candidiasis are described and presumed predisposing factors are investigated in 3 dogs. Pathologically, pyogranulomatous lesions in various organs were present in all dogs. Blastospores, pseudohyphae, and true hyphae of Candida albicans were observed with periodic acid Schiff, Gomori's methenamine silver, and immunohistochemistry. Administrations of broad-spectrum antibiotics and corticosteroids and, in one dog, parvoviral infection were thought to be predisposing factors leading to opportunistic infection. The combined effect of immunosuppressants and antibiotics might have led to Candida colonization and dissemination …

The Influence Of Cold Conditioning On The Performance Of The Broiler Chicken, Mehmet Yardimci, Erol Şengör, E. Hesna Şahi̇n, İsmai̇l Bayram, İ. Sadi̇ Çeti̇ngül

Turkish Journal of Veterinary & Animal Sciences

The effect of short-term cold exposure on the performance of 600 commercial Ross 308 male broiler chickens was examined. On the fifth and sixth days of life the trial group was exposed to 15 °C ambient temperature for 3 h, both days, while the control group was kept under conventional brooding conditions. Thereafter, both control and trial birds were exposed to standard rearing temperatures until the 21st day, and from the 21st day to slaughter they were exposed to 15 °C ambient temperature. The mortality rate was significantly lower in the trial group (5%) compared to the control group (11%). …

Ultrasonographic Monitoring Of Follicular Development, Ovulation And Corpora Lutea Formation In A Bitch, Kemal Eker, M. Rifat Salmanoğlu

Turkish Journal of Veterinary & Animal Sciences

Follicular development, ovulation and corpora lutea formation in a bitch were monitored daily by ultrasonography using a 8-MHz linear transducer. These findings were compared with vaginal cytology and changes in the peripheral serum concentrations of oestradiol-17\beta and progesterone. The follicles were identified as anechoic spherical structures on day 6 of prooestrus. The numbers of follicles imaged on day 6 of prooestrus were 3 on the left ovary and 3 on the right ovary. The average follicular size was 0.67 ± 0.06 cm on the right ovary and 0.48 ± 0.02 cm on the left ovary during the follicular phase. Apparent …

Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, And Hematological Parameters In Pregnant Akkaraman Ewes Infected With Gastro-Intestinal Parasites, Süleyman Kozat, Nazmi̇ Yüksek, Yaşar Göz, İhsan Keleş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro-intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat iron deficiency.

Evaluation Of Various Antibiotic Treatments In Calves With Infectious Bovine Keratoconjunctivitis, Murat Ki̇bar, K. Semi̇h Gümüşsoy, Ahmet Öztürk

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine whether subconjunctival administration of enrofloxacin could ameliorate a herd outbreak of infectious bovine keratoconjunctivitis (IBK) and to compare the efficacy of this regimen with the efficacy of subconjunctival administration of a combination of penicillin+streptomycin. The calves were assigned to 1 of 3 groups at the beginning of the study, and were examined 3 times weekly for 4 weeks. Calves in group 1 (n = 12) were treated with the subconjunctival administration of a combination of penicillin+streptomycin. This treatment consisted of 3 subconjunctival injections of penicillin+streptomycin every 48 h. Calves in group 2 …

Antigenic Diversity Of Bovine Viral Diarrhoea Viruses (Bvdv) Isolated In Turkey, Kadi̇r Yeşi̇lbağ, İbrahi̇m Burgu

Turkish Journal of Veterinary & Animal Sciences

Sixty BVDV field strains isolated from different regions of Turkey were antigenically characterised with a panel of 16 monoclonal antibodies (mAbs) specific to viral proteins E2 and NS2-3. MAb recognition patterns were detected by an indirect peroxidase-linked antibody assay and data were subjected to computer analysis for discussing herd specificity of viral strains isolated from immunotolerant, persistently infected (IPI) cattle. For that purpose, phylogenic and statistical analyses were performed. A high level of antigenic diversity was detected among the isolates. Diversity was also exhibited in IPI strains originating from the same herd. In some herds, viruses were divided into 2 …

Physical Fitness Of Children With Motor Learning Difficulties, Beth P. Hands, Dawne Larkin

Health Sciences Papers and Journal Articles

Children with motor learning difficulties (MLD) tend to be less physically active than their coordinated peers and one likely consequence is a reduced level of physical fitness. In this study, 52 children with MLD aged 5 to 8 years, were compared to 52 age- and gender-matched control children across a range of health and skill related fitness components. Analyses of variance revealed significantly lower scores in the group with MLD on the tests for cardiorespiratory endurance, flexibility, abdominal strength, speed, and power than the control group. Furthermore, the group with MLD had a significantly higher Body Mass Index (BMI). These …

Physical Activity Measurement Methods For Young Children: A Comparative Study, Beth P. Hands, Helen Parker, Dawne Larkin

Health Sciences Papers and Journal Articles

Many behavior patterns that impact on physical activity experiences are established in early childhood, therefore it is important that valid, reliable, and feasible measures are developed to identify children who are not developing appropriate and healthy activity habits. In this study, measures of physical activity derived by accelerometry and pedometry are compared with direct observation for twenty-four 5- and 6-year-old children. The children were monitored for 30 minutes over five consecutive days during a 30 minute free play session in their pre-primary setting. The results for all measures were significantly correlated. When compared to direct observation, the coefficient of determination …

The Analysis And Characterisation Of Rotavirus And Norovirus Circulating In The South Of Ireland, Norma Reidy

Theses

On average more than 3,000 cases of rotavirus diarrhea are detected annually amongst Irish children less than 3 years of age. Rotavirus disease occurs mainly during the winter months, with peak infections detected from February to April. The symptoms of disease are diarrhea and dehydration, with 80% of patients requiring hospitalisation, The mean duration of hospitalisation is 3-4 days, with an average cost per day of €409. Eighteen percent of infections were detected in infants less than 6 months of age, suggesting inadequate maternal derived protection from existing strains, or the existence of novel strains in Ireland with little or …

Screening For Cystic Fibrosis: The Importance Of Using The Correct Tools, Uzma Shah, Tariq Moatter

Department of Pathology and Laboratory Medicine

Background: Cystic Fibrosis (CF) is a potentially lethal genetic disorder. The most frequent mutation worldwide in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene is designated as the Delta F508 mutation. This mutation was found in only 33% of Pakistani patients studied. Since the common Pakistani mutations remain to be identified, appropriate screening tools are required to identify disease. Sweat chloride determinations remain the gold standard for diagnosing CF. This study was done to emphasize the importance of using the correct tests.
Methods: The study was conducted at the Aga Khan University Hospital. The CFTR delta F508 mutation was tested …

M. Leprae Inhibits Apoptosis In Thp-1 Cells By Downregulation Of Bad And Bak And Upregulation Of Mcl-1 Gene Expression, Zahra Hasan, Mussarat Ashraf, Ali Tayyebi, Rabia Hussain

Department of Pathology and Laboratory Medicine

Background: Virulent Mycobacterium leprae interfere with host defense mechanisms such as cytokine activation and apoptosis. The mitochondrial pathway of apoptosis is regulated by the Bcl-2 family of proteins. Expression of Fas ligand and apoptotic proteins is found in leprosy lesions and M. leprae has been shown to activate pro-apoptotic Bcl-2 genes, Bak and Bax. However, the mechanism by which M. leprae modulates apoptosis is as yet unclear. We investigated expression of apoptotic genes in THP-1 monocytes in response to infection by M. leprae and non-pathogenic M. bovis BCG.
Results: M. leprae did not induce apoptosis in THP-1 cells, while BCG …

Potential Of Vibrational Spectroscopy In The Diagnosis Of Human Tumours., Eoghan O'Faolain

Doctoral

Just fewer than 20,000 people are annually diagnosed with some form of cancer in Ireland and one in three people are likely to contract some form of cancer by age 74. With the number of cases increasing at an annual rate of 2%, the early detection and treatment of cancer is becoming increasingly important. Both IR and Raman spectroscopy offer the potential for real time, quantitative detection of cancer and even precancer. This study investigates the potential of Raman and Fourier transform infrared, both benchtop and synchrotron spectroscopies for the detection of cervical cancer. The tissue was classified and its …

The Use Of Proteomic Technologies To Identify Serum Glycoproteins For The Early Detection Of Liver And Prostate Cancers, Elizabeth Ellen Schwegler

Theses and Dissertations in Biomedical Sciences

The application of proteomic technologies to identify serum glycoproteins is an emerging technique to identify new biomarkers indicative of disease severity. Many of these newly evolving protein-profiling methodologies have evolved from previous global protein expression profiling studies such as those involving SELDI-TOF-MS technologies. Though the SELDI approach could distinguish disease from normal by utilizing protein patterns as shown herein with the HCC study of chapter II, it was unable to offer sequence information on the selected peaks, and did not have the ability to analyze the entire dynamic range of the serum/plasma proteome. To address these deficiencies, new strategies that …

The Perceptions Of Roles And Practices Of Preceptors And Their Expectations For Students In The Coordinated Program In Dietetics At Indiana State University, Carin R. Aloisio

All-Inclusive List of Electronic Theses and Dissertations

Dietetics education requires a minimum of a baccalaureate degree and 900 hours of supervised practice for eligibility of candidates to sit for the Registration Exam. The primary goals of this study are to determine perceptions of preceptors of supervised practice about their roles and practices and their expectations for students, and to compare and/or contrast these between the three main areas of dietetics: clinical, community and food service management.

Spatial Autocorrelation Of Deer-Vehicle Accidents In Vigo County, J. Scott Horn

All-Inclusive List of Electronic Theses and Dissertations

Deer-vehicle accidents (DV As) are a problem in many areas. They cause millions of dollars in property damage, personal injury, and loss of life each year. This thesis examines whether DVAs in Vigo County, Indiana during the year 2000 were spatially autocorrelated; and whether DV As selected by deer habitat were spatially autocorrelated. Specifically using Moran's I to test for spatial autocorrelation this study determined if and to what degree spatial autocorrelation existed among these DVAs. A Moran's I value of .0023 shows that the accidents occurred randomly. These DV A data were then used to plot a map which …

Bayer Ag V. Housey Pharmaceuticals: Protection For Biotechnological Research Tools Under Section 271(G) Found Wanting, Matthew Barthalow

The University of New Hampshire Law Review

[Excerpt] "Research tools, a subset of biotechnological inventions protected by process patents, are “tools that scientists use in the laboratory, including cell lines, monoclonal antibodies, reagents, animal models, growth factors, combinatorial chemistry and DNA libraries, clones and cloning tools (such as PCR), methods, laboratory equipment and machines.” Many companies base their business models on the ability to find pharmaceutical products using their proprietary drug discovery research tools. Research tools used for drug discovery ‘include bioinformatic methods for identifying the interaction of certain proteins and their association with disease, methods for confirming protein targets, screening assays to identify molecules active against …

Validity Of Frozen Section In The Diagnosis Of Breast Lumps: 5 Years Experience At The Aga Khan University Hospital., Naila Kayani, Nuzhat Sultana

Department of Pathology and Laboratory Medicine

Abstract

Objective: To determine the accuracy of frozen section in the diagnosis of breast lump. Methods: Frozen section diagnosis given in consecutive breast biopsies performed in 5 years in our laboratory was evaluated against the final pathology report of permanent sections. Results: The Aga Khan University Hospital Lab receives more than 400 cases per year for frozen section. Three hundred and nineteen consecutive frozen sections of breast lumps examined from 1999-2003 were considered for analysis. Age of patients ranged between 22 and 90 years (mean age 49 years). In 316 cases (99%) there was no difference between the frozen section …

Eating Behavior And Obesity Risk Of Youth Living In The City Of San Bernardino, California, Carolyn A. Corder

Loma Linda University Electronic Theses, Dissertations & Projects

Adolescents living in the City of San Bernardino, California, are susceptible to health risks due to high rates of violence, suicide, teen pregnancy, and poverty, which place them among the most vulnerable in the Nation to disability, early death, unhealthy offspring, illiteracy, economic disadvantage, and chronic disease. This study analyzed data from the Youth Risk Behavior (YRBS) and local surveys conducted in the City’s Unified School District from 1999 through 2001. The investigators focused on the relationship, if any, of dietary and anthropometric factors to the priority adolescent behaviors targeted for intervention by the Healthy People 2010 objectives: accidental injuries …

Mechanisms Of Hbo-Induced Neuroprotection In A Neonatal Hypoxia-Ischemia Model, John Winter Calvert

Loma Linda University Electronic Theses, Dissertations & Projects

With an incidence approaching 1/4000 live births and as high as 60% in low birth weight infants, cerebral hypoxia-ischemia during the perinatal period is the single most important cause of acute mortality and chronic disability in newborns. Herein, we tested the hypothesis that following a hypoxic-ischemic insult hyperbaric oxygen (HBO) treatment can restore high energy metabolite levels in the affected regions of the brain and through this increase in energy levels ameliorate the spread of cell death following the insult. We also investigated if an additional mechanism by which HBO affords its neuroprotection is by altering the expression of the …

Stability Of The Miniscrew-Bone Interface: Reverse Torque Values In Rabbits, Daniel John Drye

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: Miniscrews are used in orthodontics as practical temporary anchorage devices (TAD's). The purpose of this animal study was to measure reverse torque values for Absoanchor 1.2 mm and Micerium 1.5mm miniscrews at 0, 2, and 12 weeks under loaded and unloaded conditions.

Methods: Ninety-six miniscrews were placed in the femora of twelve rabbits and allowed to heal for either two or twelve weeks. Half of the fixtures were loaded immediately with NiTi open coil springs producing a force of 150-200g and the other half served as unloaded controls. For each of the healing times, two and twelve weeks, …

Growth Factor–Induced Shedding Of Syndecan-1 Confers Glypican-1 Dependence On Mitogenic Responses Of Cancer Cells, Kan Ding, Martha Lopez-Burks, José A. Sánchez-Duran, Murray Korc, Arthur D. Lander

Dartmouth Scholarship

The cell surface heparan sulfate proteoglycan (HSPG) glypican-1 is up-regulated by pancreatic and breast cancer cells, and its removal renders such cells insensitive to many growth factors. We sought to explain why the cell surface HSPG syndecan-1, which is also up-regulated by these cells and is a known growth factor coreceptor, does not compensate for glypican-1 loss. We show that the initial responses of these cells to the growth factor FGF2 are not glypican dependent, but they become so over time as FGF2 induces shedding of syndecan-1. Manipulations that retain syndecan-1 on the cell surface make long-term FGF2 responses glypican …

Loss Of Editing Activity During The Evolution Of Mitochondrial Phenylalanyl-Trna Synthetase, Hervé Roy, Jiqiang Ling, Juan D. Alfonzo, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Accurate selection of amino acids is essential for faithful translation of the genetic code. Errors during amino acid selection are usually corrected by the editing activity of aminoacyl-tRNA synthetases such as phenylalanyl-tRNA synthetases (PheRS), which edit misactivated tyrosine. Comparison of cytosolic and mitochondrial PheRS from the yeast Saccharomyces cerevisiae suggested that the organellar protein might lack the editing activity. Yeast cytosolic PheRS was found to contain an editing site, which upon disruption abolished both cis and trans editing of Tyr-tRNA^Phe. Wild-type mitochondrial PheRS lacked cis and trans editing and could synthesize Tyr-tRNA^Phe, an activity enhanced in …

Hispanic Physicians' Tobacco Intervention Practices: A Cross-Sectional Survey Study, Francisco G. Soto Mas, Richard L. Papenfuss, Holly E. Jacobson, Chiehwen E. Hsu, William M. Kane

Faculty, Staff and Student Publications

Background U.S. Hispanic physicians constitute a considerable professional collective, and they may be most suited to attend to the health education needs of the growing U.S. Hispanic population. These educational needs include tobacco use prevention and smoking cessation. However, there is a lack of information on Hispanic physicians' tobacco intervention practices, their level of awareness and use of cessation protocols, and the type of programs that would best address their tobacco training needs. The purpose of this study was to assess the tobacco intervention practices and training needs of Hispanic physicians.

Methods Data was collected through a validated survey instrument …

Rhamnolipids Modulate Swarming Motility Patterns Of Pseudomonas Aeruginosa, Nicky C. Caiazza, Robert M. Q. Shanks, G. A. O'Toole

Dartmouth Scholarship

Pseudomonas aeruginosa is capable of twitching, swimming, and swarming motility. The latter form of translocation occurs on semisolid surfaces, requires functional flagella and biosurfactant production, and results in complex motility patterns. From the point of inoculation, bacteria migrate as defined groups, referred to as tendrils, moving in a coordinated manner capable of sensing and responding to other groups of cells. We were able to show that P. aeruginosa produces extracellular factors capable of modulating tendril movement, and genetic analysis revealed that modulation of these movements was dependent on rhamnolipid biosynthesis. An rhlB mutant (deficient in mono- and dirhamnolipid production) and …

Student Employee Recruitment And Retention Through Campus Partnerships, Corinne M. Daprano, Megan L. Coyle, Peter J. Titlebaum

Health and Sport Science Faculty Publications

Recruitment and retention of student employees are important considerations for any recreational sports department, large or small, because student employees impact both the quantity and quality of programs and services. This article proposes a strategy for establishing partnerships with academic departments to aid in the recruitment and retention of student employees. These partnerships benefit students, the recreational sports department, academic departments, and university student retention efforts. Following a description of this strategy is an example of a partnership that has been successfully established at one Midwestern university.