Medicine and Health Sciences Commons^™

Healing Our Hazardous Environment, K V. Hall, B M. Afzal, Barbara Sattler

Nursing and Health Professions Faculty Research and Publications

No abstract provided.

High-Resolution Vascular Imaging Of The Rat Spine Using Liposomal Blood Pool Mr Agent, K B Ghaghada, K H J Bockhorst, S Mukundan, A V Annapragada, P A Narayana

Faculty, Staff and Student Publications

BACKGROUND AND PURPOSE: High-resolution, vascular MR imaging of the spine region in small animals poses several challenges. The small anatomic features, extravascular diffusion, and low signal-to-noise ratio limit the use of conventional contrast agents. We hypothesize that a long-circulating, intravascular liposomal-encapsulated MR contrast agent (liposomal-Gd) would facilitate visualization of small anatomic features of the perispinal vasculature not visible with conventional contrast agent (gadolinium-diethylene-triaminepentaacetic acid [Gd-DTPA]). METHODS: In this study, high-resolution MR angiography of the spine region was performed in a rat model using a liposomal-Gd, which is known to remain within the blood pool for an extended period. The imaging …

Predictive Oncology: A Review Of Multidisciplinary, Multiscale In Silico Modeling Linking Phenotype, Morphology And Growth, Sandeep Sanga, Hermann B. Frieboes, Xiaoming Zheng, Robert Gatenby, Elaine L. Bearer, Vittorio Cristini

Faculty, Staff and Student Publications

Empirical evidence and theoretical studies suggest that the phenotype, i.e., cellular- and molecular-scale dynamics, including proliferation rate and adhesiveness due to microenvironmental factors and gene expression that govern tumor growth and invasiveness, also determine gross tumor-scale morphology. It has been difficult to quantify the relative effect of these links on disease progression and prognosis using conventional clinical and experimental methods and observables. As a result, successful individualized treatment of highly malignant and invasive cancers, such as glioblastoma, via surgical resection and chemotherapy cannot be offered and outcomes are generally poor. What is needed is a deterministic, quantifiable method to enable …

Computer Simulation Of Glioma Growth And Morphology, Hermann B. Frieboes, John S Lowengrub, S Wise, X Zheng, Paul Macklin, Elaine L. Bearer, Vittorio Cristini

Faculty, Staff and Student Publications

Despite major advances in the study of glioma, the quantitative links between intra-tumor molecular/cellular properties, clinically observable properties such as morphology, and critical tumor behaviors such as growth and invasiveness remain unclear, hampering more effective coupling of tumor physical characteristics with implications for prognosis and therapy. Although molecular biology, histopathology, and radiological imaging are employed in this endeavor, studies are severely challenged by the multitude of different physical scales involved in tumor growth, i.e., from molecular nanoscale to cell microscale and finally to tissue centimeter scale. Consequently, it is often difficult to determine the underlying dynamics across dimensions. New techniques …

Estimates Of Genetic Parameters For Racing Times Of Thoroughbred Horses, Bülent Eki̇z, Ömür Koçak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to estimate the genetic parameters for racing times, which are needed for a selection program of Thoroughbred horses in Turkey. The racing records used in the study were obtained from the Turkish Jockey Club. The trait used in the study was racing time for racing distances of 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 2100, 2200, and 2400 m. The data from each racing distance were analyzed separately. Genetic parameters were estimated by REML procedure using the DFREML program. Estimates of heritability ranged from 0.177 to 0.353, depending on the racing distance. …

Pigmentation Of Rainbow Trout (Oncorhynchus Mykiss) With Carotenoids From Marigold Flower (Tagetes Erecta) And Red Pepper (Capsicum Annum), Hakan Murat Büyükçapar, Mahmut Yanar, Yasemen Yanar

Turkish Journal of Veterinary & Animal Sciences

The effects of diets including various natural carotenoid sources and synthetic astaxanthin on the pigmentation and growth of rainbow trout (Oncorhynchus mykiss) were investigated. Rainbow trout weighing about 111 g were fed diets containing 1.6%, 2.4% and 3.2% marigold flower; 4.4%, 6.6% and 8.8% red pepper; 100 mg kg^{-1} synthetic astaxanthin; and a control diet for 60 days. At the end of the experiment, synthetic astaxanthin provided the highest carotenoid accumulation in the fish flesh (6.42 mg kg^{-1}) in the groups. This was followed by red pepper (5.78 mg kg^{-1}) and marigold flower (5.59 mg kg^{-1}), each of which included …

The Effect Of Artificial Insemination Timing On The Sex Ratio Of Offspring And Fertility In Dairy Cows, Orkun Demi̇ral, Mustafa Ün, Murat Abay, Tayfur Bekyürek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effect of the interval between artificial insemination and the onset of estrus on the sex ratio of offspring and fertility in Holstein cows. Standing to be mounted behavior was considered the beginning of estrus, and artificial inseminations were performed 6 h (Group 1), 9 h (Group 2), 12 h (Group 3), and 15 h (Group 4) after the onset of estrus in cows. Overall 119 (n: 85), 107 (n: 72), 120 (n: 80) and 119 (n: 83) artificial inseminations were performed in Groups 1, 2, 3 and 4, respectively. Pregnancy rates …

Acid Adaptation Effect On Survival Of Escherichia Coli O157:H7 In Fermented Milk Products, Hali̇l Tosun, A. Kemal Seçki̇n, Şahi̇ka Aktuğ Gönül

Turkish Journal of Veterinary & Animal Sciences

The growth and survival of acid-adapted and nonadapted Escherichia coli O157:H7 were determined in strained, set-type, and synbiotic yoghurt and kefir. Acid-adapted or nonadapted E. coli O157:H7 were inoculated into the synbiotic and set-type yoghurt before and after the fermentation process. Survival of the inoculated E. coli O157:H7 in these products was examined during storage at 4 °C. Acid adaptation enhanced the survival of E. coli O157:H7 in kefir and strained yoghurt. Acid adaptation also enhanced the survival of E. coli O157:H7 in set-type yoghurt, which was inoculated after the fermentation process; however, acid-adapted cell inoculation before fermentation did not …

Chondroma In A Dog, Rifki Haziroğlu, Hasan Bi̇lgi̇li̇, Kübra A. Teri̇m Kapakin

Turkish Journal of Veterinary & Animal Sciences

An 11-year-old female terrier brought to our clinic was diagnosed with chondroma located on the caudolateral side of the right distal humerus. During the clinical examination of the dog, swelling and lameness were observed on right forelimb. Following the operation, the subject had no lameness after removing the mass. Postoperative follow-up was done by phone with the owner of the patient in 6th and 12th months, and no lameness and swelling in the region was reported. The tumoral mass was removed easily from underlying tissue without any attachment during the operation. The tissue sample from the specimen was routinely processed …

Enterotoxemia In A Cow Due To Clostridium Perfringens Type A, Hakan Kalender, Ayşe Kiliç, Eray Atil

Turkish Journal of Veterinary & Animal Sciences

Enterotoxemia was diagnosed in a 3-year-old cow that died suddenly. Lesions of hemorrhagic enteritis were seen in the small intestine during necropsy. Lethal alpha toxin of Clostridium perfringens was detected in the intestinal contents by toxin neutralization test. C. perfringens type A was isolated from the intestinal contents and typed by multiplex PCR. Enterotoxemia should be considered as a cause of sudden death in cattle in Turkey.

Acute Toxicity Of Carbaryl, Methiocarb, And Carbosulfan To The Rainbow Trout (Oncorhynchus Mykiss) And Guppy (Poecilia Reticulata), Muhammet Boran, İlhan Altinok, Erol Çapkin, Hi̇kmet Karaçam, Veysel Bi̇çer

Turkish Journal of Veterinary & Animal Sciences

The acute toxicity of carbaryl, methiocarb and carbosulfan to juvenile rainbow trout (Oncorhynchus mykiss; 1.92 ± 0.5 g) and guppies (Poecilia reticulata; 0.45 ± 0.04 g) was evaluated in 96-h toxicity tests under static conditions. Concentrations in the toxicity test ranged from 0.85 to 8.5 mg/l for carbaryl, from 0.7 to 28.0 mg/l for methiocarb, and from 0.031 to 12.5 mg/l for carbosulfan. Carbaryl and methiocarb were more toxic to rainbow trout than to guppies. However, compared with rainbow trout, guppies were more sensitive to carbosulfan. The incidence of mortality in fish increased significantly (P < 0.05) with increasing pesticide concentrations. The time required to kill 50% (LT_{50}) of the rainbow trout at the lowest concentrations of carbaryl, methiocarb, and carbosulfan was 51 h 12 min, 74 h 35 min, and 107 h 57 min, respectively. As insecticide concentrations were increased, LT_{50} values decreased (P < 0.05).

Isolation Of Salmonella Spp. In Camel Sausages From Retail Markets In Aydın, Turkey, And Polymerase Chain Reaction (Pcr) Confirmation, Gökben Özbey, Fi̇li̇z Kök, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

A total of 100 camel sausage samples from different retail markets in Aydın, located in southwest Turkey, were examined for the presence of Salmonella spp. by culture method. After sausage samples was preenriched in buffered peptone water and incubated 18-20 h at 37 °C. were transferred to Rappaport-Vassiliadis broth and Selenite broth, respectively, and incubated at 42 and 37 °C, respectively. After 24 and 48 h of incubation, one loopful from each of the enriched broths was streaked onto plates of Salmonella-Shigella agar and xylose lysine deoxycholate agar and incubated at 37 °C for 24 h. A 572-bp fragment of …

The Use Of Hazelnut Meal As A Substitute For Soybean Meal In The Diets Of Rainbow Trout (Oncorhynchus Mykiss), Özlem Bi̇lgi̇n, Ali̇ Türker, Ahmet Adem Teki̇nay

Turkish Journal of Veterinary & Animal Sciences

Hazelnut meal as a substitute for soybean meal in extruded feeds of rainbow trout was investigated. The performance of extruded feeds containing hazelnut meal as a substitute for 20% of soybean meal (Diet 2) and 30% of soybean meal (Diet 3) was compared to a nutritionally balanced control (Diet 1) (a commercial trout feed formulation). Each diet was fed to 3 replicate groups (initial BW: 36 ± 1.37 g; mean ± SD); the fish were fed the equivalent of 2% of their body weight, 2 times daily (0800 and 1600) for 64 days at 15 ± 0.11 °C under a …

Changes In Some Blood Biochemical And Haematological Parameters In Goats After Aminoglycoside And Aminocyclitol Treatment At Therapeutic Doses, Toncho Dinev, Dimitrinka Zapryanova, Lubomir Lashev

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine the changes in some blood biochemical and haematological parameters in healthy female goats after a 5-day parenteral treatment with gentamicin (4 mg/kg), tobramycin (5 mg/kg), amikacin (10 mg/kg), kanamycin (10 mg/kg), apramycin (20 mg/kg), and spectinomycin (20 mg/kg). The results indicated that aminoglycosides caused more severe alterations than aminocyclitols, especially on those parameters related to renal function. There was a tendency toward a rise in plasma creatinine and urea levels after aminoglycoside treatment, as well as a rise in urea levels after aminocyclitol administration. These antibiotics caused decreases in plasma or …

Histopathological Changes After Transplantation Of Stored Osteochondral Allografts Into Articular Cartilage Lesions In Dogs, Zbigniew Adamiak, Wojciech Brzeski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate histopathological changes in the recipient site after transplantation of stored osteochondral allografts into articular cartilage defects in 8 dogs. In all dogs a single osteochondral allograft was transplanted into articular cartilage lesions. The results of the completed surgeries include a 12-month follow-up study. It was found that the stored osteochondral allografts were very firmly connected to the recipient tissue and no arthritis changes were noted.

Seroepidemiology Of Equine Influenza Virus Infection In Turkey, Veysel Soydal Ataseven, Janet Mary Daly

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the seroepidemiology of equine influenza infection in Turkey, measured using the haemagglutination inhibition (HI) assay. Serum samples were obtained from 623 equids aged older than 4 years from 5 different geographical regions in Turkey during the years 2003-2005. Antibodies against equine influenza virus (EIV) were found in 194 (31.1%) of the 623 sera. Seropositivity rates were detected as 41.8% (171/409), 12.8% (11/86), and 9.4% (12/128) in horses, mules, and donkeys, respectively. The data revealed that EIV circulates in Turkey at a relatively high rate. Differences in seropositivity rates in Equidae from different …

Accidental Poisoning From Lantana Camara (Cherry Pie) Hay Fed To Ostriches (Struthio Camelus), Ross G. Cooper

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Serum Biochemical Changes In Experimental Gambian Trypanosomosis. Ii. Assessing Hepatic And Renal Dysfunction, J. N. Abenga, V. O. Anosa

Turkish Journal of Veterinary & Animal Sciences

Serum biochemical changes were assessed to determine hepatic and renal function in 4 vervet monkeys experimentally infected with Trypanosoma brucei gambiense, the causative agent of sleeping sickness in West and Central Africa. Parameters examined included serum bilirubin, blood urea nitrogen (BUN), and cholesterol levels. Significant increases in the total bilirubin and BUN levels occurred from the second week post-infection (PI) (P Keywords: Serum biochemistry, trypanosomosis, human, monkeys

The Effects Of Planting Time And Combination On The Nutrient Composition And Digestible Dry Matter Yield Of Four Mixtures Of Vetch Varieties Intercropped With Barley, N. Tuğba Bi̇ngöl, M. Aki̇f Karsli, İ. Hakki Yilmaz, Duran Bolat

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the chemical composition, digestibility, energy content, and digestible dry matter yield of 4 mixtures of different vetch varieties intercropped with the same barley at 3 different planting times under Eastern Anatolian conditions. Four different vetch species, i.e. common (Farukbey-2001), Hungarian (Tarım Beyazı-98), hairy (Selçuklu-2002), and wooly pod (Segmen-2002), intercropped with barley (Tokak-157) were utilized. The mixtures of vetches intercropped with barley were planted on 7 October 2003 (I), 7 November 2003 (II), and 10 April 2004 (III), and each variety/planting time was randomly assigned to 3 replications. The mixtures of vetches intercropped …

The Prevalence Of Borrelia Burgdorferi Sensu Lato In Ixodes Ricinus Ticks In The Sinop Region Of Turkey, Turabi̇ Güneş, Serpi̇l Kaya, Ömer Poyraz, Aynur Engi̇n

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in order to determine the prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks, which are the primary vectors of this spirochete, as well as to investigate the effect of I. ricinus population density on the prevalence of B. burgdorferi s.l. Ticks were collected by blanket dragging in 13 different forest regions of Sinop, Turkey, and its vicinity. Ticks were identified under a stereomicroscope. I. ricinus (n = 334), I. hexagonus (n = 5), Rhipicephalus spp. (n = 10), Dermacentor spp. (n = 7), and Hyalomma spp. (n = 5) were collected. Direct immunofluorescence was …

Effect Of Sodium Lactate On The Microbiological Quality And Shelf Life Of Sausages, Enver Bariş Bi̇ngöl, Kami̇l Bostan

Turkish Journal of Veterinary & Animal Sciences

This study was performed to investigate the effects of various levels of sodium lactate on the microbiological quality and shelf life of sausages, and determine whether it can be used as a nitrite alternative. For this purpose, 0%, 0.6%, 1.2%, and 1.8% NaL and 0.125% NaNO_2 were added to sausage samples. Samples were vacuum-packaged and stored at 4 °C for 60 days and periodically analyzed for their sensorial properties, microbial counts (aerobic plate count, lactic acid bacteria, yeast-mould, and sulphite reducing anaerobic bacteria), and pH values. Sensorial characteristics were not altered with NaL addition; the flavor of the samples was …

The Effects Of Fermented Soybean Meal On Growth Performance And Immune Characteristics In Weaned Piglets, Xin Liu, Jianlei Feng, Zirong Xu, Yaping Lu, Yuanyuan Liu

Turkish Journal of Veterinary & Animal Sciences

This experiment was conducted to determine the effects of fermented soybean meal (FSBM) on growth performance and immune characteristics of piglets. In all, 60 crossbred piglets (Duroc x Landrace x Yorkshire) were allocated to 2 dietary treatments; 3 replications of 10 pigs were used for each treatment, and all piglets were weaned at 35 days and fed for 23 days. The results showed that average daily gain (ADG) improved (1.44% vs. 1.36%) in piglets fed the FSBM diet and feed gain ratio (FGR) decreased by 5.56% (P < 0.05) compared to the controls (soybean meal treatment). The level of serum IgG decreased 27.2% (P < 0.01) in piglets fed FSBM. Lymphocytes from whole blood had a lower proliferative response to concanavalin A (ConA) (P < 0.01) and lipopolysaccharide (LPS) (P < 0.05). Meanwhile, the splenocytes of piglets fed FSBM also showed lower proliferative response to ConA (P < 0.05) and LPS (P < 0.01). The results indicated that FSBM was beneficial to growth performance and decreased the immune response to soybean protein in piglets.

Determination Of Clostridium Perfringens Toxin-Types In Sheep With Suspected Enterotoxemia In Kars Province, Turkey, Hali̇l İbrahi̇m Gökce, Oktay Genç, Mahmut Sözmen, Gürbüz Gökçe

Turkish Journal of Veterinary & Animal Sciences

The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Kars province. For this purpose, 260 dead sheep with suspected enterotoxemia and 35 clinically healthy sheep (controls) of different ages and breeds were used. Intestinal contents of each sheep were collected and used to determine alpha, beta, and epsilon toxins of C. perfringens using enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT). Based on ELISA and LAT, 220 (84.61%) and 152 (58.46%) sheep, respectively, were positive for C. perfringens toxins. In contrast, 40 (15.38%) sheep were toxin-negative based …

Immunohistochemical Expression Of Estrogen And Progesterone Receptors In Oviduct Adenocarcinomas In Laying Hens, M. Özgür Özyi̇ği̇t, Gürsel Sönmez

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to demonstrate the presence of estrogen and progesterone receptors (ERs and PRs, respectively) in oviduct adenocarcinomas in laying hens. In all, 32 oviduct adenocarcinomas (18 primary tumors and 14 intestinal metastases) from Babcock B-380 laying hens 16-18 months old were stained with commercially available monoclonal antibodies against mouse-anti-human ERs and mouse-anti-human PRs. In the microscopic examination, 13 tumors (7 oviduct adenocarcinomas and 6 intestinal metastases) stained positively for ERs and 12 tumors (4 oviduct adenocarcinomas and 8 intestinal metastases) gave a positive reaction to PR antibody. The staining pattern of the tumor cells with …

Effects Of Breed And Lactation Period On Some Characteristics And Free Fatty Acid Composition Of Raw Milk From Damascus Goats And German Fawn X Hair Goat B_1 Crossbreds, Zehra Güler, Mahmut Keski̇n, Tuğrul Masatçioğlu, Sabri̇ Gül, Osman Bi̇çer

Turkish Journal of Veterinary & Animal Sciences

The effects of breed and period of lactation on milk composition were investigated. For this purpose, 8 crossbred (3/4 German fawn 1/4 Hair goat) and 10 Damascus goats were kept under identical management conditions. Lactation period affected milk yield and milk composition in both goat breeds. Milk yield, lactation duration, and mean solid and fat values showed differences between the breeds. The mean values of total solid and fat of milk during lactation were 13.82 ± 0.294% and 4.55 ± 0.223% for the crossbreds, and 12.90 ± 0.179% and 4.02 ± 0.142% for Damascus goats, respectively. Although the percentages of …

A Proteome-Wide Protein Interaction Map For Campylobacter Jejuni, Jodi R. Parrish, Jingkai Yu, Guozhen Liu, Julie A. Hines, Jason E. Chan, Bernie A. Mangiola, Huamei Zhang, Svetlana Pacifico, Farshad Fotouhi, Victor J. Dirita, Trey Ideker, Phillip Andrews, Russell L. Finley Jr

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Data from large-scale protein interaction screens for humans and model eukaryotes have been invaluable for developing systems-level models of biological processes. Despite this value, only a limited amount of interaction data is available for prokaryotes. Here we report the systematic identification of protein interactions for the bacterium Campylobacter jejuni, a food-borne pathogen and a major cause of gastroenteritis worldwide.

Results

Using high-throughput yeast two-hybrid screens we detected and reproduced 11,687 interactions. The resulting interaction map includes 80% of the predicted C. jejuni NCTC11168 proteins and places a large number of poorly characterized proteins into networks that provide initial …

Infant Mortality Trends Among Georgia Residents, 1995-2003: Targeting Healthy People’S 2010 Goals, Diana Sturges, Laura H. Gunn, Padmini Shankar, Shrikrishna Shroff

Health Sciences & Kinesiology: Faculty Publications

Population-based trends in infant mortality among Georgia Residents between 1995 and 2003 were assessed on characteristics such as race, birth weight, neonatal and post-neonatal periods, and cause of death. A statistical analysis was conducted to show that the Georgia infant mortality rate (IMR) remained constant throughout the study period and averaged 8.67 per 1,000 live births. The analysis revealed racial disparities, with an IMR ranging from 6.03 in white infants to 13.76 in black infants, with less than one percent (0.86%) change, on average, among the differences between black and white mortality rates across the nine-year period. The disparities were …

Changes In Energy Expenditure With Ingestion Of High Protein, High Fat Versus High Protein, Low Fat Meals Among Underweight, Normal Weight, And Overweight Females., Amy Jo Riggs, Sareen S. Gropper, Barry D. White

Health Sciences & Kinesiology: Faculty Publications

Background: Metabolic rate is known to rise above basal levels after eating, especially following protein consumption. Yet, this postprandial rise in metabolism appears to vary among individuals. This study examined changes in energy expenditure in response to ingestion of a high protein, high fat (HPHF) meal versus an isocaloric high protein, low fat (HPLF) meal in underweight, normal weight, or overweight females (n = 21) aged 19–28 years.

Methods: Energy expenditure, measured using indirect calorimetry, was assessed before and every 30 minutes for 3.5 hours following consumption of the meals on two separate occasions. Height and weight were …

Increasing Serum 25-Hydroxyvitamin D As A Possible Deterrent To The Onset Of Multiple Sclerosis, Nicholas J. Perretti

Seton Hall University Dissertations and Theses (ETDs)

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Dna Multiplex Hybridization On Microarrays And Thermodynamic Stability In Solution: A Direct Comparison, Daniel J. Fish, M. Todd Horne, Greg P. Brewood, Jim P. Goodarzi, Saba Alemayehu, Ashwini Bhandiwad, Robert P. Searles, Albert S. Benight

Chemistry Faculty Publications and Presentations

Hybridization intensities of 30 distinct short duplex DNAs measured on spotted microarrays, were directly compared with thermodynamic stabilities measured in solution. DNA sequences were designed to promote formation of perfect match, or hybrid duplexes containing tandem mismatches. Thermodynamic parameters DeltaH degrees , DeltaS degrees and DeltaG degrees of melting transitions in solution were evaluated directly using differential scanning calorimetry. Quantitative comparison with results from 63 multiplex microarray hybridization experiments provided a linear relationship for perfect match and most mismatch duplexes. Examination of outliers suggests that both duplex length and relative position of tandem mismatches could be important factors contributing to …