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Articles 35041 - 35070 of 42125

Full-Text Articles in Medicine and Health Sciences

Comparison Of Spermatological Characteristics And Biochemical Seminal Plasma Parameters Of Normozoospermic And Oligoasthenozoospermic Bulls Of Two Breeds, Mesut Çevi̇k, Pürhan Barbaros Tuncer, Umut Taşdemi̇r, Taner Özgürtaş Jan 2007

Comparison Of Spermatological Characteristics And Biochemical Seminal Plasma Parameters Of Normozoospermic And Oligoasthenozoospermic Bulls Of Two Breeds, Mesut Çevi̇k, Pürhan Barbaros Tuncer, Umut Taşdemi̇r, Taner Özgürtaş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the differences in spermatological and seminal plasma biochemical characteristics between normozoospermic and oligoasthenozoospermic bulls. A total of 8 bulls were used and they were divided into 4 groups: Holstein normozoospermic (HN) and oligoasthenozoospermic (HO), and Brown Swiss normozoospermic (BSN) and oligoasthenozoospermic (BSO). Spermatological parameters investigated included semen volume, color, concentration, motility, and percentage of abnormal sperm. Biochemical parameters measured in seminal plasma included pH, glucose, urea, creatinine, uric acid, cholesterol (CH), triglyceride, Mg, inorganic P (P_i), Ca, Na, K, and Cl ion concentrations. Colorimetric and ion selective electrode methods were used for …


Modelling The Protein And Amino Acid Requirements Of The Greater Rhea (Rhea Americana), James Sales Jan 2007

Modelling The Protein And Amino Acid Requirements Of The Greater Rhea (Rhea Americana), James Sales

Turkish Journal of Veterinary & Animal Sciences

Protein and amino acid requirements of the Greater rhea (Rhea americana) have been estimated from growth data available for this species, and assumptions derived with other closely related species. Differences in body weight and growth pattern caused considerable higher protein and amino acid requirements for males compared to females after 3 months of age. Whereas the absolute protein requirement is comparable between males (148 g/day) and females (144.5 g/day) at 50 days of age, 21.8 g/day protein is needed by males at 500 days of age compared to 12.2 g/day for females. Limitations include the lack of knowledge on feed …


Biodosepr-2006 Consensus Committee Report On Biodosimetric Methods To Evaluate Radiation Doses At Long Times After Exposure, Steven L. Simon, Ian Bailiff, André Bouville, Paola Fattibene, Ruth A. Kleinerman, David C. Lloyd, Stephen W.S. Mckeever, Alexander Romanyukha, Alexander V, Sevan’Kaev, James D. Tucker, Albrecht Wieser Jan 2007

Biodosepr-2006 Consensus Committee Report On Biodosimetric Methods To Evaluate Radiation Doses At Long Times After Exposure, Steven L. Simon, Ian Bailiff, André Bouville, Paola Fattibene, Ruth A. Kleinerman, David C. Lloyd, Stephen W.S. Mckeever, Alexander Romanyukha, Alexander V, Sevan’Kaev, James D. Tucker, Albrecht Wieser

Uniformed Services University of the Health Sciences

The requirements for biodosimetric techniques used at long times after exposure, i.e., 6 months to more than 50 years, are unique compared to the requirements for methods used for immediate dose estimation. In addition to the fundamental requirement that the assay measures a physical or biologic change that is proportional to the energy absorbed, the signal must be highly stable over time to enable reasonably precise determinations of the absorbed dose decades later. The primary uses of these biodosimetric methods have been to support long-term health risk (epidemiologic) studies or to support compensation (damage) claims. For these reasons, the methods …


Reduced Migration, Altered Matrix And Enhanced Tgfβ1 Signaling Are Signatures Of Mouse Keratinocytes Lacking Sdc1, Mary Ann Stepp, Yueyuan Liu, Sonali Pal-Ghosh, Rosalyn A. Jurjus, Gauri Tadvalka, Adith Sekaran, Kristen Losicco, Li Jiang, Melinda Larsen, Luowei Li, Stuart H. Yuspa Jan 2007

Reduced Migration, Altered Matrix And Enhanced Tgfβ1 Signaling Are Signatures Of Mouse Keratinocytes Lacking Sdc1, Mary Ann Stepp, Yueyuan Liu, Sonali Pal-Ghosh, Rosalyn A. Jurjus, Gauri Tadvalka, Adith Sekaran, Kristen Losicco, Li Jiang, Melinda Larsen, Luowei Li, Stuart H. Yuspa

Biological Sciences Faculty Scholarship

We have reported previously that syndecan-1 (Sdc1)-null mice show delayed re-epithelialization after skin and corneal wounding. Here, we show that primary keratinocytes obtained from Sdc1-null mice and grown for 3-5 days in culture are more proliferative, more adherent and migrate more slowly than wt keratinocytes. However, the migration rates of Sdc1-null keratinocytes can be restored to wild-type levels by replating Sdc1-null keratinocytes onto tissue culture plates coated with fibronectin and collagen I, laminin (LN)-332 or onto the matrices produced by wild-type cells. Migration rates can also be restored by treating Sdc1-null keratinocytes with antibodies …


Ocpat: An Online Codon-Preserved Alignment Tool For Evolutionary Genomic Analysis Of Protein Coding Sequences, Guozhen Liu, Monica Uddin, Munirul Islam, Morris Goodman, Lawrence I. Grossman, Roberto Romero, Derek E. Wildman Jan 2007

Ocpat: An Online Codon-Preserved Alignment Tool For Evolutionary Genomic Analysis Of Protein Coding Sequences, Guozhen Liu, Monica Uddin, Munirul Islam, Morris Goodman, Lawrence I. Grossman, Roberto Romero, Derek E. Wildman

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Rapidly accumulating genome sequence data from multiple species offer powerful opportunities for the detection of DNA sequence evolution. Phylogenetic tree construction and codon-based tests for natural selection are the prevailing tools used to detect functionally important evolutionary change in protein coding sequences. These analyses often require multiple DNA sequence alignments that maintain the correct reading frame for each collection of putative orthologous sequences. Since this feature is not available in most alignment tools, codon reading frames often must be checked manually before evolutionary analyses can commence.

Results

Here we report an online codon-preserved alignment tool (OCPAT) that generates …


Whole Genome Expression Profiling Reveals A Significant Role For Immune Function In Human Abdominal Aortic Aneurysms, Guy M. Lenk, Gerard Tromp, Shantel Weinsheimer, Zoran Gatalica, Ramon Berguer, Helena Kuivaniemi Jan 2007

Whole Genome Expression Profiling Reveals A Significant Role For Immune Function In Human Abdominal Aortic Aneurysms, Guy M. Lenk, Gerard Tromp, Shantel Weinsheimer, Zoran Gatalica, Ramon Berguer, Helena Kuivaniemi

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Abdominal aortic aneurysms are a common disorder with an incompletely understood etiology. We used Illumina and Affymetrix microarray platforms to generate global gene expression profiles for both aneurysmal (AAA) and non-aneurysmal abdominal aorta, and identified genes that were significantly differentially expressed between cases and controls.

Results

Affymetrix and Illumina arrays included 18,057 genes in common; 11,542 (64%) of these genes were considered to be expressed in either aneurysmal or normal abdominal aorta. There were 3,274 differentially expressed genes with a false discovery rate (FDR) ≤ 0.05. Many of these genes were not previously known to be involved in …


Immunohistochemical Diagnosis Of Aspergillosis In Adult Turkeys, Enver Beytut Jan 2007

Immunohistochemical Diagnosis Of Aspergillosis In Adult Turkeys, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to apply immunohistochemistry, using a commercially available monoclonal antibody, for the diagnosis of aspergillosis in adult turkeys. Eight adult turkeys were studied. The lungs revealed grossly a few yellowish-white granulomas. Histopathologically, the granulomas were distributed in the pulmonary parenchyma or occasionally located around the airways. A few fungal hyphae were detected within the necrotic debris of the granulomas in the lungs using routine fungal stains. Immunohistochemical staining performed on lung sections from all of the turkeys disclosed fungal hyphae and their fragments clearly within the necrotic debris and within the cytoplasm of macrophages …


Design, Synthesis, And Evaluation Of 10-N-Substituted Acridones As Novel Chemosensitizers In Plasmodium Falciparum, Jane X. Kelly, Martin J. Smilkstein, Roland A. Cooper, Kristin D. Lane, Robert A. Johnson, Aaron Janowsky, Rozalia A. Dodean, David J. Hinrichs, Rolf Winter, Michael Riscoe Jan 2007

Design, Synthesis, And Evaluation Of 10-N-Substituted Acridones As Novel Chemosensitizers In Plasmodium Falciparum, Jane X. Kelly, Martin J. Smilkstein, Roland A. Cooper, Kristin D. Lane, Robert A. Johnson, Aaron Janowsky, Rozalia A. Dodean, David J. Hinrichs, Rolf Winter, Michael Riscoe

Biological Sciences Faculty Publications

A series of novel 10-N-substituted acridones, bearing alkyl side chains with tertiary amine groups at the terminal position, were designed, synthesized, and evaluated for the ability to enhance the potency of quinoline drugs against multidrug-resistant (MDR) Plasmodium falciparum malaria parasites. A number of acridone derivatives, with side chains bridged three or more carbon atoms apart between the ring nitrogen and terminal nitrogen, demonstrated chloroquine (CQ)-chemosensitizing activity against the MDR strain of P. falciparum (Dd2). Isobolograrn analysis revealed that selected candidates demonstrated significant synergy with CQ in the CQ-resistant (CQR) parasite Dd2 but only additive (or indifferent) interaction in the CQ-sensitive …


On A Multinational Assessment Of Rotavirus Disease In Europe, David O. Matson Jan 2007

On A Multinational Assessment Of Rotavirus Disease In Europe, David O. Matson

Community & Environmental Health Faculty Publications

Rotaviruses were discovered in the 1960s in animals and in the 1970s in humans; the latter discovery was made by an intrepid group who performed duodenal biopsies on children with acute gastroenteritis (AGE) [1]. By the late 1970s, data already clearly indicated that rotavirus was the cause of the annual winter peak of AGE affecting young children, as well as a frequent cause of severe gastroenteritis in various animal species (e.g., [2–5]). Use of the retrospectroscope clarified or left as tantalizing the suggestion that rotaviruses were the cause of the annual “winter vomiting syndrome” first described in children in 1910 …


Potential For Stimulating Host Anti-Tumor Immune Response Via Rnai-Mediated Local Foxp3 Knockdown, N. Klaiber Jan 2007

Potential For Stimulating Host Anti-Tumor Immune Response Via Rnai-Mediated Local Foxp3 Knockdown, N. Klaiber

Chemistry & Biochemistry Faculty Publications

Neoplastic growths represent a unique challenge for the host immune system. As they are indeed derived from self, many of the same mechanisms operating to prevent autoimmunity also provide an umbrella beneath which malignant cells are free to proliferate.1 Central among these immune regulatory boundaries are an influential subset of lymphocytes known as T regs. Hypothesized to exist decades ago, yet eluding definitive characterization until relatively recently, T regs have been demonstrated to play a crucial role in the proper functioning of the immune system as a whole. They may also, however, represent one of the primary obstacles to successful …


A Spatial-Temporal Approach To Surveillance Of Prostate Cancer Disparities In Population Subgroups, Chiehwen E. Hsu, Francisco Soto Mas, Jerry A. Miller, Ella T. Nkhoma Jan 2007

A Spatial-Temporal Approach To Surveillance Of Prostate Cancer Disparities In Population Subgroups, Chiehwen E. Hsu, Francisco Soto Mas, Jerry A. Miller, Ella T. Nkhoma

Faculty, Staff and Student Publications

BACKGROUND: Prostate cancer mortality disparities exist among racial/ethnic groups in the United States, yet few studies have explored the spatiotemporal trend of the disease burden. To better understand mortality disparities by geographic regions over time, the present study analyzed the geographic variations of prostate cancer mortality by three Texas racial/ethnic groups over a 22-year period.

METHODS: The Spatial Scan Statistic developed by Kulldorff et al was used. Excess mortality was detected using scan windows of 50% and 90% of the study period and a spatial cluster size of 50% of the population at risk. Time trend was analyzed to examine …


Bioelectric Effects Of Intense Nanosecond Pulses, Karl H. Schoenbach, Barbara Y. Hargrave, Ravindra P. Joshi, Juergen F. Kolb, Richard Nuccitelli, Christopher J. Osgood, Andrei G. Pakhomov, Michael W. Stacey, James R. Swanson, Jody A. White, Shu Xiao, Jue Zhang, Stephen J. Beebe, Peter F. Blackmore, E. Stephen Buescher Jan 2007

Bioelectric Effects Of Intense Nanosecond Pulses, Karl H. Schoenbach, Barbara Y. Hargrave, Ravindra P. Joshi, Juergen F. Kolb, Richard Nuccitelli, Christopher J. Osgood, Andrei G. Pakhomov, Michael W. Stacey, James R. Swanson, Jody A. White, Shu Xiao, Jue Zhang, Stephen J. Beebe, Peter F. Blackmore, E. Stephen Buescher

Bioelectrics Publications

Electrical models for biological cells predict that reducing the duration of applied electrical pulses to values below the charging time of the outer cell membrane (which is on the order of 100 ns for mammalian cells) causes a strong increase in the probability of electric field interactions with intracellular structures due to displacement currents. For electric field amplitudes exceeding MV/m, such pulses are also expected to allow access to the cell interior through conduction currents flowing through the permeabilized plasma membrane. In both cases, limiting the duration of the electrical pulses to nanoseconds ensures only nonthermal interactions of the electric …


The Effects Of 17Β-Estradiol And Tamoxifen On Cell Cycle Distribution And F-Actin Expression In Mcf-7 Cells, Kirk Kangaloo, Latoya Jenkins, Nicole Clarke, Samir S. Raychoudhury Jan 2007

The Effects Of 17Β-Estradiol And Tamoxifen On Cell Cycle Distribution And F-Actin Expression In Mcf-7 Cells, Kirk Kangaloo, Latoya Jenkins, Nicole Clarke, Samir S. Raychoudhury

Journal of the South Carolina Academy of Science

No abstract provided.


Using Microenvironmental Effects: Loading, Activity, And Stereoselectivity Relative To Polymer Bound Nabh4 Reagents, James R. Blanton Jan 2007

Using Microenvironmental Effects: Loading, Activity, And Stereoselectivity Relative To Polymer Bound Nabh4 Reagents, James R. Blanton

Journal of the South Carolina Academy of Science

No abstract provided.


The Journal Of Undergraduate Research: Volume 05 Jan 2007

The Journal Of Undergraduate Research: Volume 05

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 5.


Functional Properties Of L-Glutamate Regulation In Anesthetized And Freely Moving Mice, Kevin N. Hascup Jan 2007

Functional Properties Of L-Glutamate Regulation In Anesthetized And Freely Moving Mice, Kevin N. Hascup

Theses and Dissertations--Neuroscience

L-glutamate (Glu) is the predominant excitatory neurotransmitter in the mammalian central nervous system with involvement encompassing learning and memory, cognition, plasticity, and motor movement. Dysregulation of the glutamatergic system is implicated in several neurological disorders including Parkinson’s disease, Alzheimer’s disease, Huntington’s disease and amyotrophic lateral sclerosis. The mechanisms underlying these neurological disorders are not clear, but evidence suggests that abnormal Glu neurotransmission plays a role. Elevated levels of Glu in the synaptic cleft overstimulate the N-methyl-Daspartate receptor leading to excitotoxicity, which causes neuronal loss in chronic neurological diseases. What is less understood is the source for the elevated Glu levels. …


Xenobiotic Transporters In Lactating Mammary Epithelial Cells: Predictions For Drug Accumulation In Breast Milk, Philip Earle Empey Jan 2007

Xenobiotic Transporters In Lactating Mammary Epithelial Cells: Predictions For Drug Accumulation In Breast Milk, Philip Earle Empey

Theses and Dissertations--Pharmacy

Recent literature has established that breast cancer resistance protein (ABCG2) is upregulated during lactation and is responsible for the greater than predicted accumulation of many drugs in breast milk. The objectives of this project were (1) to investigate the role of this transporter in the reported apically-directed nitrofurantoin flux in the CIT3 cell culture model of lactation, (2) to develop a mathematical model for drug transfer into breast milk to relate initial flux rates, steady-state concentrations, efflux ratios, and in vivo milk to serum ratios (M/S) and (3) to identify xenobiotic transporters that are highly expressed, and therefore potentially important …


Self-Consistent Analyses For Potential Conduction Block In Nerves By An Ultrashort High-Intensity Electric Pulse, R. P. Joshi, A. Mishra, Q. Hu, K. H. Schoenbach, A. Pakhomov Jan 2007

Self-Consistent Analyses For Potential Conduction Block In Nerves By An Ultrashort High-Intensity Electric Pulse, R. P. Joshi, A. Mishra, Q. Hu, K. H. Schoenbach, A. Pakhomov

Bioelectrics Publications

Simulation studies are presented that probe the possibility of using high-field (>100kV ∕ cm), short-duration (∼50ns) electrical pulses for nonthermal and reversible cessation of biological electrical signaling pathways. This would have obvious applications in neurophysiology, clinical research, neuromuscular stimulation therapies, and even nonlethal bioweapons development. The concept is based on the creation of a sufficiently high density of pores on the nerve membrane by an electric pulse. This modulates membrane conductance and presents an effective "electrical short" to an incident voltage wave traveling across a nerve. Net blocking of action potential propagation can then result. A continuum approach based …


Controlling Listeria Monocytogenes On Ready-To-Eat Poultry Products Using Carboxymethylcellulose Film Coatings Containing Green Tea Extract (Gte) Combined With Nisin And Malic Acid, Brittany Adams, N. Hettiarachchy, M. G. Johnson Jan 2007

Controlling Listeria Monocytogenes On Ready-To-Eat Poultry Products Using Carboxymethylcellulose Film Coatings Containing Green Tea Extract (Gte) Combined With Nisin And Malic Acid, Brittany Adams, N. Hettiarachchy, M. G. Johnson

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The ability to control Listeria monocytogenes on ready-to-eat poultry products using carboxymethyl-cellulose film coatings containing green tea extract (GTE), malic acid (M), nisin (N), and their combinations was evaluated. The antimicrobials (GTE: 1.0%, nisin: 10,000 IU/g, malic acid: 1.0%) were incorporated alone or in combination into a carboxymethyl cellulose film coating. Pre-inoculated, fully cooked chicken pieces (~1g, 1cm x 1cm x 1cm) were coated with the film solution. The coated chicken pieces were stored at 4°C and the inhibitory activity against Listeria monocytogenes was evaluated at 0, 7, 14, 21, and 28 days. The highest inhibitory activity was found in …


Functional Neuroimaging And The Law: Trends And Directions For Future Scholarship, Stacey A. Tovino Jan 2007

Functional Neuroimaging And The Law: Trends And Directions For Future Scholarship, Stacey A. Tovino

Faculty Articles

Under the umbrella of the burgeoning neurotransdisciplines, scholars are using the principles and research methodologies of their primary and secondary fields to examine developments in neuroimaging, neuromodulation, and psychopharmacology. The path for advanced scholarship at the intersection of law and neuroscience may clear if work across the disciplines is collected and reviewed and outstanding and debated issues are identified and clarified. In this article, I organize, examine and refine a narrow class of burgeoning neurotransdiscipline scholarship; that is, scholarship at the interface of law and functional magnetic resonance imaging.


Comparative Predictive Value Of Three Prognostic Markers--S-Phase Fraction, Pcna And Mitotic Count On Axillary Lymph Node Metastasis In Carcinoma Breast, Shahid Pervez, Muhammad Nadeem Khan, Muhammad Israr Nasir Jan 2007

Comparative Predictive Value Of Three Prognostic Markers--S-Phase Fraction, Pcna And Mitotic Count On Axillary Lymph Node Metastasis In Carcinoma Breast, Shahid Pervez, Muhammad Nadeem Khan, Muhammad Israr Nasir

Department of Pathology and Laboratory Medicine

Background: Axillary lymph node metastasis is the single most important prognostic factor in carcinoma of the breast. Therefore, prognostic markers that may reliably predict probability of lymph node (LN) metastases are of great value. This study was conducted to compare the predictive value of two novel prognostic / proliferative markers i.e. S-phase fraction (SPF) and proliferating cell nuclear antigen (PCNA) in parallel with mitotic index.

Methods: Data of consecutive cases of infiltrating ductal carcinoma (IDC) breast diagnosed from July 2003 to July 2004 at the section of the Histopathology, The Aga Khan University Hospital, Karachi, were reviewed. A total of …


Preparation Of Neuraminidase-Specific Antiserum From The H9n2 Subtype Of Avian Influenza Virus, Ali Nazari Shirvan, Mehran Moradi, Mehdi Aminian, Rasool Madani Jan 2007

Preparation Of Neuraminidase-Specific Antiserum From The H9n2 Subtype Of Avian Influenza Virus, Ali Nazari Shirvan, Mehran Moradi, Mehdi Aminian, Rasool Madani

Turkish Journal of Veterinary & Animal Sciences

Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs. The harvested suspension was concentrated by polyethylene glycol 6000. Concentrated samples were layered onto sucrose gradient (30%-60%). Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100 across 30% sucrose gradient. NA was isolated from HA and other viral proteins by affinity chromatography on N-(p-aminophenyl)oxamic acid. Fractions that had high NA activity and did not show HA activity were pooled and analyzed by neuraminidase inhibition and SDS-PAGE. No viral protein bands were detected, except for a single band in the position of …


Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen Jan 2007

Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen

Turkish Journal of Veterinary & Animal Sciences

In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …


Protein Profile And Plasmid Content Of Lactococcus Lactis Subsp. Lactis Ll52 And Lactococcus Lactis Subsp. Cremoris Lc79 Strains Under Several Stress Conditions, Rahmi̇ Lale, Çağla Tükel, Mustafa Akçeli̇k Jan 2007

Protein Profile And Plasmid Content Of Lactococcus Lactis Subsp. Lactis Ll52 And Lactococcus Lactis Subsp. Cremoris Lc79 Strains Under Several Stress Conditions, Rahmi̇ Lale, Çağla Tükel, Mustafa Akçeli̇k

Turkish Journal of Veterinary & Animal Sciences

Differences in the protein and plasmid content of 2 Lactococcus lactis strains, L. lactis subsp. lactis LL52 and L. lactis subsp. cremoris LC79, under the stresses of high and low temperature, osmotic shock, and low pH were determined. We identified 3 new proteins with molecular masses of 16.0, 29.4, and 45.0 kDa as high temperature stress response specific in strain LL52. High temperature stress did not cause any changes in the protein content of strain LC79. Proteins that were specific for salt stress and low pH stress responses ranged between 16.0 and 40.5 kDa, and 24.8 and 107.5 kDa, respectively, …


Detection Of Antibodies Produced In Quails (Coturnix Coturnix Japonica) Against Mycoplasma Gallisepticum With Different Serological Tests, Süheyla Türkyilmaz, Fethi̇ye Çöven, Seza Eski̇i̇zmi̇rli̇ler Jan 2007

Detection Of Antibodies Produced In Quails (Coturnix Coturnix Japonica) Against Mycoplasma Gallisepticum With Different Serological Tests, Süheyla Türkyilmaz, Fethi̇ye Çöven, Seza Eski̇i̇zmi̇rli̇ler

Turkish Journal of Veterinary & Animal Sciences

The objectives of this study were to isolate and identify Mycoplasma gallisepticum in quails with clinical and pathological signs of chronic respiratory disease (CRD), and to compare the sensitivity and specificity of commonly used serological tests that are used for the detection of M. gallisepticum antibodies. The study included 20 Japanese quails 10-weeks old with respiratory distress, nasal discharge, mortality and swollen infraorbital sinuses. There was no bacteriological agent isolated. All of the 20 sera samples obtained from the quails were evaluated with AGID, RSA, HI, and ELISA; 2 (10%), 5 (25%), 10 (50%), and 17 (85%) sera samples were …


The Seroprevalence Of Listeria Monocytogenes In Sport Horses Bred In Ankara Province, H. Zeynep Güçlü, K. Zafer Karaer, Cahi̇t Babür, Selçuk Kiliç Jan 2007

The Seroprevalence Of Listeria Monocytogenes In Sport Horses Bred In Ankara Province, H. Zeynep Güçlü, K. Zafer Karaer, Cahi̇t Babür, Selçuk Kiliç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the seroprevalence of Listeria monocytogenes in horses bred for sport in the province of Ankara. Sera collected from 100 clinically healthy horses were tested for the presence of L. monocytogenes antibodies using the Osebold absorption test. Agglutination titers >= 1:100 were accepted as positive results and a seropositivity rate was determined. Of the 100 serum samples, 62 were seropositive for L. monocytogenes antibodies detected at dilutions of 1:100 (n = 29, 46.7%), 1:200 (n = 31, 50%), and 1:400 (n = 2, 3.2%).


Anatomical And Histological Studies Of Accessory Adrenal Nodules In Caspian Miniature Horses, Javad Ahmadpanahi Jan 2007

Anatomical And Histological Studies Of Accessory Adrenal Nodules In Caspian Miniature Horses, Javad Ahmadpanahi

Turkish Journal of Veterinary & Animal Sciences

Microscopic and macroscopic structures of accessory adrenal nodules in Caspian miniature horses (5 males and 5 females) were studied. The accessory adrenal nodules were round or oval and yellow to red. Their maximum diameters varied from 0.1 to 0.5 cm. These nodules were found near the kidneys and adrenal glands, but they were in close contact with the adrenal gland. The accessory adrenal nodules were similar to the adrenal cortex microscopically, and from the outer layer to the inner layer they contained glomerulosa, intermedia, and fasciculata. The adrenal nodules were surrounded by a capsule of collagenous dense connective tissue as …


Effect Of Feeding Supplemental Tallow On The Performance Of Lactating Nili-Ravi Buffaloes, Haq Nawaz, Muhammad Yaqoob, Muhammad Abdullah Jan 2007

Effect Of Feeding Supplemental Tallow On The Performance Of Lactating Nili-Ravi Buffaloes, Haq Nawaz, Muhammad Yaqoob, Muhammad Abdullah

Turkish Journal of Veterinary & Animal Sciences

In order to determine the feeding value of tallow for lactating Nili-Ravi buffaloes, 4 buffaloes were fed 0%, 2%, 4%, or 6% tallow in a 4 X 4 Latin square experiment. The intakes of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) decreased (P < 0.01), but the intakes of ether extract (EE) (P < 0.01) and digestible energy (DE) (P < 0.05) increased with increasing levels of tallow in the diets. Intake of net energy for lactation (NE_L) did not differ (P < 0.01) with varying levels of supplemental tallow. Daily milk yield increased (P < 0.01) from 11.00 to 13.2 kg/day. Production of 4% fat corrected milk (FCM), solid corrected milk (SCM), and energy corrected milk (ECM) increased quadratically (P < 0.05) with the increasing level of tallow in the diets. Milk fat content and total milk fat increased linearly (P < 0.01) and quadratically (P < 0.05), respectively, as the level of tallow in the diet increased. There was no difference in protein and lactose content due to feeding tallow. Total solid content increased quadratically (P < 0.05) from 16.45% to 17.67% but solid not fat (SNF) percentages did not vary with varying levels of tallow. The gross energy (GE) of the milk increased from 1096 to 1160 Kcal/kg (P < 0.01) with increasing levels of tallow in the diets. Energetic efficiency of milk production improved in a quadratic (P < 0.05) manner with 2%, 4%, and 6% tallow, with the highest being 47.6% with 4% supplemental tallow. The proportion of C_{8:0} to C_{16:1} fatty acids (FA) decreased from 53.66% to 35.52%, whereas the concentration of C_{18:0} to C_{20:0} increased (44.93% to 62.84%) in the milk fat of buffaloes fed diets containing different levels of tallow. Gains in body weight (BW) were higher (P < 0.05) in buffaloes fed diets containing supplemental tallow as compared to those fed the control diet. This study suggests that tallow up to 6% of diet DM is a suitable fat supplement as an economical energy source for lactating buffaloes.


Allelic Frequency Of Kappa-Casein And Beta-Lactoglobulin In Indian Crossbred (Bos Taurus X Bos Indicus) Dairy Bulls, Rajesh K. Patel, Jenabhai B. Chauhan, Krishna M. Singh, Kalpesh J. Soni Jan 2007

Allelic Frequency Of Kappa-Casein And Beta-Lactoglobulin In Indian Crossbred (Bos Taurus X Bos Indicus) Dairy Bulls, Rajesh K. Patel, Jenabhai B. Chauhan, Krishna M. Singh, Kalpesh J. Soni

Turkish Journal of Veterinary & Animal Sciences

A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. In the study, 2 types of alleles A and B and 3 types of genotypes AA, BB, and AB for kappa-casein and beta-lactoglobulin were observed. The results …


Left Abdominal Cryptorchidism In A Native Goat, Gülsüm Özyi̇ği̇t Jan 2007

Left Abdominal Cryptorchidism In A Native Goat, Gülsüm Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

An 18-month-old native goat with 25 kg bodyweight submitted to the Department of Anatomy from a farm for use in student practicals was diagnosed with cryptorchidism. In macroscopical examination, the cryptorchid testis was near the left kidney. The cryptorchid testis was small, firm, and 31 x 30 mm in size and weighed 11 g, while the right testis, located in the scrotum, was 53 x 29 mm in size and weighed 37 g. Microscopically there was tubular hypoplasia and the capsule of the testis (tunica albuginea) was thicker in the left cryptorchid testis. Cryptorchidism is seen more often in horses, …