Medicine and Health Sciences Commons^™

Role Of Sec61alpha In The Regulated Transfer Of The Ribosome-Nascent Chain Complex From The Signal Recognition Particle To The Translocation Channel, Weiqun Song, David Raden, Elisabet Mandon, Reid Gilmore

Elisabet Mandon

Targeting of ribosome-nascent chain complexes to the translocon in the endoplasmic reticulum is mediated by the concerted action of the signal recognition particle (SRP) and the SRP receptor (SR). Ribosome-stripped microsomes were digested with proteases to sever cytoplasmic domains of SRalpha, SRbeta, TRAM, and the Sec61 complex. We characterized protein translocation intermediates that accumulate when Sec61alpha or SRbeta is inactivated by proteolysis. In the absence of a functional Sec61 complex, dissociation of SRP54 from the signal sequence is blocked. Experiments using SR proteoliposomes confirmed the assembly of a membrane-bound posttargeting intermediate. These results strongly suggest that the Sec61 complex regulates …

A Retrospective Study Of Borrelia Burgdorferi Antibodies In Dogs In Minnesota, Aneela Durrani, Sagar Goyal

Turkish Journal of Veterinary & Animal Sciences

In order to determine the seroprevalence of Borrelia burgdorferi antibodies in suspected dogs in Minnesota, the present study examines the database of the Veterinary Diagnostic Laboratory at the University of Minnesota over a 10 year period (2001-June 2010). A total of 1081 serum samples from dogs suspected of having borreliosis were submitted by 112 private veterinary clinics. The samples were tested using an indirect fluorescent antibody (IFA) test. Samples with titers of =>1:320 were considered positive. The rate of seroprevalence of antibodies was 88%. The increase in the rate of yearly seroprevalence of Borrelia burgdorferi antibodies in dogs was …

A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung

Turkish Journal of Veterinary & Animal Sciences

A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the …

A Comparison Of Different Kinds Of Malassezia Species In Healthy Dogs And Dogs With Otitis Externa And Skin Lesions, Samaneh Eidi, Ali Reza Khosravi, Shahram Jamshidi

Turkish Journal of Veterinary & Animal Sciences

Malassezia yeasts are commensal organisms on the skin of warm-blooded vertebrates. The lipid-dependent Malassezia species have recently been cultured from veterinary specimens. The present study investigated and compared different Malassezia species in the skin and external ear canal of healthy and diseased dogs. The sampling in the study was carried out on 152 animals, comprising 90 samples from the diseased group and 62 samples from the healthy group. All of the samples were determined by cytological examination and fungal culture. The isolated yeasts were identified by their morphological features as well as their physiological characteristics. The culture results were positive …

Methicillin Resistance In Staphylococcus Pseudintermedius, Chad Christopher Black

Doctoral Dissertations

Staphylococcus pseudintermedius affecting dogs is analogous to S. aureus on humans, acting as both normal flora and opportunistic pathogen. Methicillin resistance in S. pseudintermedius is recent, with the first documented occurrence of an isolate bearing the methicillin resistance gene, mecA, in 1999. This gene encodes penicillin binding protein 2a, which renders all beta-lactam drugs ineffective and functions as a “gateway” antibiotic resistance determinant. In the presence of ineffective antibiotics, opportunities for mutational events and acquisition of mobile genetic elements increase as microbial densities increase, often leading to multi-drug resistance. Methicillin-resistant S. pseudintermedius (MRSP) infections have become increasingly common. For …

Comparison Of The Results Of Serum Total Protein Concentration Measured By 3 Methods: Preliminary Results, Teodora M. Georgieva, Dimitrinka S. Zapryanova, Evgeniya V. Dishlyanova, Stoyan I. Tanev, Ivan P. Georgiev, Maria I. Andonova, Ivan N. Kanelov, Lazarin V. Lazarov, Petya I. Koleva

Turkish Journal of Veterinary & Animal Sciences

The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.10^9 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro …

Supervillin (P205): A Novel Membrane-Associated, F-Actin-Binding Protein In The Villin/Gelsolin Superfamily, Kersi N. Pestonjamasp, Robert K. Pope, J. D. Wulfkuhle, Elizabeth J. Luna

Elizabeth J. Luna

Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here the purification and initial characterization of p205, a 205-kD protein from bovine neutrophil plasma membranes that binds to the sides of actin filaments in blot overlays. p205 is a tightly bound peripheral membrane protein that cosediments with endogenous actin in sucrose gradients and immunoprecipitates. Amino acid sequences were obtained from SDS-PAGE-purified p205 and used to generate antipeptide antibodies, immunolocalization data, and cDNA sequence information. The intracellular localization of p205 in MDBK cells is a function of cell density and adherence state. In subconfluent cells, p205 …

Seasonal Frequency Of Ectoparasite Infestation In Dogs From Shiraz, Southern Iran, Sardar Jafari Shoorijeh, Abbas Rowshan Ghasrodashti, Amin Tamadon, Nasrin Moghaddar, Mohammad Amin Behzadi

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the prevalence of canine ectoparasite infestation in pet dogs from the Shiraz area of southern Iran, with no known exposure to insecticides. In all, 160 dogs were examined for ectoparasites in each of the 4 seasons and samples were collected. The most common ectoparasite was Ctenocephalides canis, which infested 22 of the 160 dogs. Pulex irritans was identified on 2 of the dogs and 142 Rhipicephalus sanguineus ticks were found on 13 dogs. Trichodectes canis was observed on 2 dogs and 8 dogs had Hippobosca flies, which were seen mostly in spring. …

The Effect Of Transcervical Intrauterine And Intravaginal Insemination Methods On Fertility In Dogs, Ali̇ Daşkin, Necmetti̇n Teki̇n, Ergun Akçay

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of transcervical and intravaginal artificial insemination on the pregnancy rate in dogs. Semen was collected by the massage method from dogs brought to our department. Collected semen was examined in the laboratory and stored in liquid nitrogen after dilution with Tris extender. During the study 31 bitches brought to our department for artificial insemination were used. One of the bitches was not evaluated. Oestrus was detected by vaginal cytology and the bitches were inseminated twice during the oestrus period. Fifteen of the 30 bitches were inseminated transcervically and the other 15 …

An Experimental Study On The Effect Of Acidic Drinks On Gingival Health In Dogs, Recep Orbak, Ertunç Dayi, Mustafa Atasever

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to conduct a preliminary investigation on the effects of cola drink consumption on gingival health in dogs and thereafter to come up with an opinion as to what extent cola consumption has an impact on gingival health in humans. The study was carried out on 20 healthy dogs (range 2.3-12 years; mean 8.3 years). The dogs were randomly divided into two equal groups. The control group was not given anything containing acid, while the experimental group was given classic Coca Cola® (pH: 2.4) (500 ml daily). All the dogs were fed a number of …

Immunohistochemical Localization Of Cytokeratin, Vimentin And Alpa-Smooth Muscle Actin Micro And Intermediate Filaments In Canine Mammary Tumors, Ümi̇t H. Mi̇lli̇, Rifki Haziroğlu, Yilmaz Aydin, M. Yavuz Gülbahar

Turkish Journal of Veterinary & Animal Sciences

Forty-six formalin-fixed paraffin-embedded canine mammary tumors, 15 benign and 31 malignant, were studied immunohistochemically. Mouse monoclonal anti-alpha-cytokeratin 18, mouse monoclonal anti-alpha-cytokeratin (pan cytokeratin), mouse monoclonal anti-alpha-smooth muscle actin and mouse monoclonal anti-vimentin antibodies were used. The ductal and acinar epithelium stained positively for cytokeratin 18 and pan cytokeratin, although there was no immunoreactivity in some tumors. Metaplastic squamous cells in two solid carcinomas were positive for both cytokeratin 18 and pan cytokeratin. It was a notable finding that there were unreactive epithelial cells in some tumors for broad spectrum cytokeratin antibody (pan cytokeratin). Myofibroblasts in benign and malignant tumors were …

Effects Of Megestrol Acetate Treatment On Dogs With Prolonged Estrus, Ayhan Baştan, Seli̇m Aslan

Turkish Journal of Veterinary & Animal Sciences

A total of 22 dogs of different breeds with persistent estrus, between the ages of 1.5 and 6, were used. Diagnoses were made by anamnesis, general examination and vaginal smear. Vulvar edema, colour and volume of vaginal discharge, convolution of the mucosa and volume of secretion were recorded before and after treatment. Dogs were treated with 25 mg megestrol acetate (Niagestine, 25 mg/ml, Novo). Clinical manifestations disappeared within 3-7 days. Vaginal discharge ceased in 3.5±2.5 days and vulvar edema ceased in 3.5±1.6 days. In only one dog, the treatment was not effective after 15 days. The vulva was still swollen …

Determination Of Kinetic, Structural And Functional Changes In The Hearts Of Dogs With Experimentally Introduced Myocardial Infarction Using M-Mode And 2-D Echocardiography, Utku Bakirel, Tarik Bi̇lal

Turkish Journal of Veterinary & Animal Sciences

In this study, the kinetic, structural and functional changes of the ventriculus in dogs with myocardial infarction introduced experimentally were examined using M-Mode and two dimensional (2-D) echocardiography. 21 healthy, mixed-breed dogs of both genders (16 females and 5 males) and of various ages were used in this study. Different coronary arteries were ligated in each dog with silk sutures and the dogs were grouped accordingly: left distalis descending coronary artery (Group I, n=7), right distalis descending coronary artery (Group II, n=7) and both left and right distalis decending coronary artery (Group III, n=7). All the dogs were examined using …

A Potential Champ? [Distemper In Dogs], F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

THE mother of this pup was an eight-year-old sheep dog and in the eyes of her owner she was a champion and well worth her keep.

She was perhaps slowing down a little, so the best looking pup out of the last litter had been kept as a possible replacement.