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Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy
Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy
Turkish Journal of Veterinary & Animal Sciences
Water buffalo breeding is practiced in various provinces of Turkey, and is also included in the National Breeding Project. It was aimed in this study to investigate polymorphisms on exon 4?intron 5 and exon 4 only of the kappa-casein (CSN3) gene for milk yields of water buffalo bred in Sivas Province. Blood samples were taken from 135 water buffaloes. The phenol/chloroform method for DNA extraction was conducted on collected blood. Polymerase chain reaction (PCR) was used to amplify CSN3-specific regions, and amplified products were run using 2% agarose gel electrophoresis (AGE). Various restriction endonucleases (RE) were utilized in respect to …
Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz
Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the genetic variation of MYF-5 and STAT5A genes in Anatolian water buffalo which was the only buffalo breed reared in Turkey by using the PCR-RFLP method. In this study, 120 Anatolian water buffalo were examined. After PCR amplification for MYF-5 gene, 512 bp PCR products were digested with TaqI enzyme. Although no AA genotype was found, the frequency of GG and AG genotypes were 0.77 and 0.33. PCR products of 215 bp for STAT5A gene were digested with AvaI enzyme and showed that all of the Anatolian water buffalo examined had monomorphic …