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Full-Text Articles in Medicine and Health Sciences
Genetic Variation At The Olr1, Anxa9, Myf5, Ltf, Igf1, Lgb, Csn3, Pit1, Mbl1,Cacna2d1, And Abcg2 Loci In Turkish Grey Steppe, Anatolian Black, And Eastanatolian Red Cattle, Özden Çobanoğlu, Sena Ardiçli
Genetic Variation At The Olr1, Anxa9, Myf5, Ltf, Igf1, Lgb, Csn3, Pit1, Mbl1,Cacna2d1, And Abcg2 Loci In Turkish Grey Steppe, Anatolian Black, And Eastanatolian Red Cattle, Özden Çobanoğlu, Sena Ardiçli
Turkish Journal of Veterinary & Animal Sciences
Native breeds are excellent sources of genetic variation. Anatolian native breeds are relatives of the first cattle domesticated and are ancestors of many European breeds. Therefore, this study aimed to evaluate the genetic variation of OLR1, ANXA9, MYF5, LTF, IGF1, LGB, CSN3, PIT1, MBL1, CACNA2D1, and ABCG2 markers in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red cattle. The analysis included 367 cattle and the genotyping was performed by the PCR-RFLP. Population genetics indices including heterozygosity, the number of effective alleles, the polymorphism information content, fixation index, and the level of possible variability realization, and moreover, the genetic diversity …
Caprine Mhc Gene Polymorphism And Its Association With Endoparasitic Infestation(Haemonchus Contortus) In Indian Goat Breeds, Thirunavukkarasu Sbalamurugan, Pushpendra Kumar, Kush Shrivastava, Chinmoy Mishra, Om Prakash, Amit Kumar, Anuj Chauhan, Nihar Ranjan Sahoo, Manjit Panigrahi, Bharat Bhushan, Arvind Prasad, Ilayakumar Kaveriyappan, Sharavanan Velusamy
Caprine Mhc Gene Polymorphism And Its Association With Endoparasitic Infestation(Haemonchus Contortus) In Indian Goat Breeds, Thirunavukkarasu Sbalamurugan, Pushpendra Kumar, Kush Shrivastava, Chinmoy Mishra, Om Prakash, Amit Kumar, Anuj Chauhan, Nihar Ranjan Sahoo, Manjit Panigrahi, Bharat Bhushan, Arvind Prasad, Ilayakumar Kaveriyappan, Sharavanan Velusamy
Turkish Journal of Veterinary & Animal Sciences
The present study examined the variability of MHC class II DRB exon 2 gene using restriction fragment length polymorphism analysis of PCR-amplified fragments PCR-(RFLP), and its association with Haemonchus contortus infestation in Salem Black and Tellicherry goat population. Animals were naturally exposed to mixed infestation of endoparasites, predominantly Haemonchus contortus. Pooled fecal coproculture and larval identification showed predominant presence of haemonchus (L3) larva. Fecal egg count (FEC) and packed cell volume (PCV) were used as indicator traits. All the three studied loci, TaqI, BsaI, and BsaHI, were polymorphic having two alleles and three genotypes. The loci showed low to moderate …
Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy
Assessment Of Polymorphism On Kappa-Casein Gene Of Anatolian Water Buffalo Breedusing The Pcr-Rflp Method, Yusuf Özşensoy
Turkish Journal of Veterinary & Animal Sciences
Water buffalo breeding is practiced in various provinces of Turkey, and is also included in the National Breeding Project. It was aimed in this study to investigate polymorphisms on exon 4?intron 5 and exon 4 only of the kappa-casein (CSN3) gene for milk yields of water buffalo bred in Sivas Province. Blood samples were taken from 135 water buffaloes. The phenol/chloroform method for DNA extraction was conducted on collected blood. Polymerase chain reaction (PCR) was used to amplify CSN3-specific regions, and amplified products were run using 2% agarose gel electrophoresis (AGE). Various restriction endonucleases (RE) were utilized in respect to …
Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz
Polymorphism Of The Stat5a And Myf-5 Genes In Anatolian Water Buffalo, Fadi̇me Daldaban, Korhan Arslan, Esma Gamze Aksel, Bi̇lal Akyüz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the genetic variation of MYF-5 and STAT5A genes in Anatolian water buffalo which was the only buffalo breed reared in Turkey by using the PCR-RFLP method. In this study, 120 Anatolian water buffalo were examined. After PCR amplification for MYF-5 gene, 512 bp PCR products were digested with TaqI enzyme. Although no AA genotype was found, the frequency of GG and AG genotypes were 0.77 and 0.33. PCR products of 215 bp for STAT5A gene were digested with AvaI enzyme and showed that all of the Anatolian water buffalo examined had monomorphic …
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev
Turkish Journal of Veterinary & Animal Sciences
The most prospective and actual trend in farm animal breeding is studying gene polymorphisms affecting the productive traits. The objective of this investigation was to study the effect of polymorphisms in intron 1 of the swine POU1F1 gene on number of days to 100 kg (days to 100 kg), length of body (LB), backfat thickness (BF), and number of piglets born alive (NBA). Research was conducted on purebred pigs of Landrace (n = 80) and Duroc (n = 100) and crossbred pigs (Landrace x Large White) (n = 192). Insertions/deletions in intron 1 of the POU1F1 gene were defined by …
Characterization Of Porcine Adiponectin Gene (Adipoq) Polymorphismsand Their Association With Litter Size, Xiaodong Zhang, Ning Zhou, Jie Zhou, Ning Wang, Xiang Qin, Hui Dong, Yueyun Ding, Zongjun Yin
Characterization Of Porcine Adiponectin Gene (Adipoq) Polymorphismsand Their Association With Litter Size, Xiaodong Zhang, Ning Zhou, Jie Zhou, Ning Wang, Xiang Qin, Hui Dong, Yueyun Ding, Zongjun Yin
Turkish Journal of Veterinary & Animal Sciences
Litter size is one of the most important economic traits for pig production as it is directly related to production efficiency. To evaluate the effects of the adiponectin (ADIPOQ) gene on porcine litter size, we investigated ADIPOQ polymorphisms in three breeds, Wannan Black pigs, Berkshire pigs, and BW pigs (crossbred pigs produced from mating male Berkshire pigs and female Wannan Black pigs), using the PCR-SSCP method. Three SNPs (c. 178G > A, c. 1165A > G, and c. 1138G > A) were identified. In Wannan Black pigs, association analysis indicated that the c. 178G > A SNP genotype was significantly associated with an effect …
Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan
Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan
Turkish Journal of Veterinary & Animal Sciences
DIO2 was studied as a candidate gene for reproduction in sheep. Three pairs of primers were designed to detect single nucleotide polymorphisms (SNPs) of exon 2 of DIO2 in nonseasonal and seasonal reproduction breeds. The polymorphisms were detected using SeqMan II and analyzed by PCR-RFLP. The sheep DIO2 gene was cloned and its mutations were detected in five sheep breeds whose reproductive seasonality and litter sizes were different. Association analyses were performed to reveal the relationships between SNPs and reproductive seasonality as well as litter size in sheep. The results showed that a 1041-bp DNA fragment of the sheep DIO2 …
Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram
Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the genotypes of bovine growth hormone and to investigate their association with daily weight gain in the Zavot cattle breed. The bovine growth hormone (GH) genotypes of 45 male Zavot cattle were identified by PCR-RFLP. The effect of the bovine growth hormone gene on daily weight gain was estimated from a dataset obtained from cattle up to the age of 9 months. The mean differences of carcass weight or carcass yield among the genotype groups were assessed by analysis of covariance (ANCOVA). Two alleles for bovine growth hormone locus, L and V, …
Polymorphisms Of Ovine Prion Protein (Prp) Gene In Pramenka Sheepbreed Population In Bosnia And Herzegovina, Ervin Zecevic, Admir Dokso, Amra Kazic, Muhamed Brka
Polymorphisms Of Ovine Prion Protein (Prp) Gene In Pramenka Sheepbreed Population In Bosnia And Herzegovina, Ervin Zecevic, Admir Dokso, Amra Kazic, Muhamed Brka
Turkish Journal of Veterinary & Animal Sciences
Transmissible spongiform encephalopathies (TSEs) are a group of diseases that affect the nervous system and lead to death. The putative infectious agent is the host-encoded prion protein (PrP) and it appears that the development of scrapie in sheep is closely related to polymorphisms in the host PrP gene. The aim of this study was to investigate three well-known polymorphisms in exon 3 of the PrP gene (at codons 136, 154, and 171) that have a clear and significant effect on scrapie in sheep. Four other polymorphisms (at codons 145, 185, 231, and 237) that are yet undefined were also investigated. …
Association Of Molecular Polymorphism In Exon 2 Of Ovar-Drb1 Gene With Weight Traits In Iranian Makuie Sheep Breed, Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian
Association Of Molecular Polymorphism In Exon 2 Of Ovar-Drb1 Gene With Weight Traits In Iranian Makuie Sheep Breed, Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian
Turkish Journal of Veterinary & Animal Sciences
Exon 2 of the Ovar-DRB1 gene of 90 Makuie sheep was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Ten alleles and 18 genotypes were identified. The frequencies of the genotypes were 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487, and 0.0365 for AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ, and AV, respectively. Allele frequencies were 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854, and 0.0183 for A, B, E, F, I, M, O, P, …
Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü
Mva I Pcr-Rflp Identifies Single Nucleotide Polymorphism Of The Alpha-Lactalbumin Gene In Some Goat Breeds Reared In Turkey, Özgecan Korkmaz Ağaoğlu, Mustafa Saatci, Özkan Elmaz, Mehmet Çolak, Mesi̇h Kocamüftüoğlu, Emel Zeytünlü
Turkish Journal of Veterinary & Animal Sciences
Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, …
Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh
Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh
Turkish Journal of Veterinary & Animal Sciences
In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (
Polymorphism Evaluation Of Various Genomic Loci In The Kıvırcık Sheep Breed Of Turkey, Harun Ceri̇t, Ahmet Altinel, Özkan Elmaz, Kozet Avanus
Polymorphism Evaluation Of Various Genomic Loci In The Kıvırcık Sheep Breed Of Turkey, Harun Ceri̇t, Ahmet Altinel, Özkan Elmaz, Kozet Avanus
Turkish Journal of Veterinary & Animal Sciences
: In recent years regions which exhibit many genetic polymorphisms for the DNA of cattle, sheep and goats have been discovered. The aim of this study is to analyse the polymorphism of various microsatellites, which have been previously defined as polymorphism for sheep, and to evaluate the use of the Kıvırcık sheep population, which is indigenous to Turkey in relationship testing. The results have been evaluated for heterozygosity, power exclusion, matching probability, power discrimination, and paternity index. For DNA extraction, the chelex and phenol-chloroform-isoamyl alcohol methods were applied to the blood taken from animals. After measuring the amount and degree …
Determination Of The Allele Frequency Of Some Genomic Loci In A Holstein Cattle Population And Its Importance In Individual Identification, Harun Ceri̇t
Turkish Journal of Veterinary & Animal Sciences
Nowadays, the use of DNA sequences with genetic techniques and DNA polymorphism as genetic markers is increasing very rapidly. In this study, we examined the frequencies of seven different polymorphic loci in the cattle population of İstanbul University Veterinary Medicine Research and Application Farm. Thus, our aim was to examine and calculate the power of exclusion, match probability and power of discrimination of the seven loci, and examine the usefulness of the involved loci in identification tests. For DNA extraction, Chelex and Phenol-Chloroform-Isoamil alcohol methods were applied to the blood taken from animals. After measuring the amount and purity degree …
Potassium And Haemoglobin Polymorphism In Hair Goat Raising In Antalya Province, Kemal Karabağ, M. Soner Balcioğlu, M. Zi̇ya Firat, H. İbrahi̇m Yolcu
Potassium And Haemoglobin Polymorphism In Hair Goat Raising In Antalya Province, Kemal Karabağ, M. Soner Balcioğlu, M. Zi̇ya Firat, H. İbrahi̇m Yolcu
Turkish Journal of Veterinary & Animal Sciences
In this study, whole blood potassium and haemoglobin (Hb) polymorphism were investigated in the Hair goats raised in the Antalya province. The separation of erythrocyte haemoglobin types was carried out using horizontal starch-gel electrophoresis and the determination of the potassium level was done using an atomic absorption spectrometer in randomly chosen whole blood samples of 188 Hair goats. The gene frequency for the higher potassium (KH) was 0.985±0.038. As a result of this research, two haemoglobin gene alleles (HbA, HbB) were determined. The estimation of HbA allele gene frequency was 0.785±0.021. The Hair goat population investigated in this study was …
Estimation Of Genetic Distance In Meat And Layer Pure Lines Using Randomly Amplified Polymorphic Dna, Rahşan İvgi̇n, Güldehen Bi̇lgen
Estimation Of Genetic Distance In Meat And Layer Pure Lines Using Randomly Amplified Polymorphic Dna, Rahşan İvgi̇n, Güldehen Bi̇lgen
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to evaluate genetic similarities and distances among pure lines of poultry using the RAPD technique. In this study four layer and three meat type pure lines were used. Venous blood samples from five males and five females of each line were collected from the venae cutenea ulnaris. The RAPD technique was used to generate a DNA fingerprint of lines. Initially, a total of 35 primers having arbitrary sequences were used for RAPD analysis. The amplicons were resolved on 1.5% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands …
Transferrin Polymorphism In Hair Goat Breeds Raised In The Bursa Region, Cengi̇z Elmaci, Mehmet Koyuncu, Erdoğan Tuncel
Transferrin Polymorphism In Hair Goat Breeds Raised In The Bursa Region, Cengi̇z Elmaci, Mehmet Koyuncu, Erdoğan Tuncel
Turkish Journal of Veterinary & Animal Sciences
In this study transferrin polymorphism was investigated in hair goat breeds raised in the Bursa region by using 148 blood samples of hair goat. The separation of transferrin types was carried out using horizontal starch-gel electrophoresis. The frequencies of transferrin alleles were calculated to be 0.54 ±0.029 and 0.46 ±0.029 for Tf A and Tf B , respectively. Differences between the expected and observed number of transferrin genotypes were insignificant.
Relationships Between Milk Yield Traits And Milk Protein Polymorphism In Brown Swiss Cattle In Turkey, Mahmut Doğan, Ali̇ Kaygisiz
Relationships Between Milk Yield Traits And Milk Protein Polymorphism In Brown Swiss Cattle In Turkey, Mahmut Doğan, Ali̇ Kaygisiz
Turkish Journal of Veterinary & Animal Sciences
In this study, effects of milk protein polymorphism on milk yield traits were investigaed in Brown Swiss cattle. Starch Gel Elektroforesis methods were used for milk protein typing. The data were evaluated by Least squares and Maximum Likelihood Computer Program. Least squares means were found to be 4029.46±251.73 kg for 305 days milk yield, 301.41±14.84 days for lactation length. Effects of \alpha-s_1-cazein, \beta-Lactoglobulin and \kappa-cazein types on milk yield traits were non-significant (p>0.05), while efect of \beta-Casein types were significant (p
Blood And Milk Protein Polymorphism And Their Effects On Various Production Traits In Simmental Cows, Özel Şekerden, Faruk Doğrul, Hüseyi̇n Erdem
Blood And Milk Protein Polymorphism And Their Effects On Various Production Traits In Simmental Cows, Özel Şekerden, Faruk Doğrul, Hüseyi̇n Erdem
Turkish Journal of Veterinary & Animal Sciences
This research was carried out to determine the transferrin (Tf) and haemoglobine (Hb) and milk protein (alfa-S_1-casein, beta casein, capa casein, beta lactoglobuline) types, and relationships between Tf and Hb types and milk protein genotypes with various characteristics. The relationship between Tf types and milk yields of control milking and control day were only statistically significant (P0.05). None of the investigated characteristics were effected by milk protein genetic variants at statistically significant level.