Medicine and Health Sciences Commons^™

The Role Of Dendritic Cell Subsets And Innate Immunity In The Pathogenesis Of Type 1 Diabetes And Other Autoimmune Diseases, Jeffrey D. Price, Kristin V. Tarbell

Nebraska Center for Virology: Faculty Publications

Dendritic cells (DCs) are key antigen-presenting cells that have an important role in autoimmune pathogenesis. DCs control both steady-state T cell tolerance and activation of pathogenic responses. The balance between these two outcomes depends on several factors, including genetic susceptibility, environmental signals that stimulate varied innate responses, and which DC subset is presenting antigen. Although the specific DC phenotype can diverge depending on the tissue location and context, there are four main subsets identified in both mouse and human: conventional cDC1 and cDC2, plasmacytoid DCs, and monocyte-derived DCs. In this review, we will discuss the role of these subsets in …

Whole-Genome Sequencing Of Kaposi’S Sarcoma-Associated Herpesvirus From Zambian Kaposi’S Sarcoma Biopsy Specimens Reveals Unique Viral Diversity, Landon Olp, Adrien Jeanniard, Clemence Marimo, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent for Kaposi’s sarcoma (KS). Both KSHV and KS are endemic in sub-Saharan Africa where approximately 84% of global KS cases occur. Nevertheless, whole-genome sequencing of KSHV has only been completed using isolates from Western countries—where KS is not endemic. The lack of whole-genome KSHV sequence data from the most clinically important geographical region, sub-Saharan Africa, represents an important gap since it remains unclear whether genomic diversity has a role on KSHV pathogenesis. We hypothesized that distinct KSHV genotypes might be present in sub-Saharan Africa compared to Western countries. Using a KSHV-targeted enrichment …

Widespread Detection Of Antibodies To Leptospira In Feral Swine In The United States, K. K. Pedersen, K. L. Pabilonia, T. D. Anderson, S. N. Bevins, C. R. Hicks, J. M. Kloft, Thomas J. Deliberto

USDA Wildlife Services: Staff Publications

As feral swine continue to expand their geographical range and distribution across the United States, their involvement in crop damage, livestock predation, and pathogen transmission is likely to increase. Despite the relatively recent discovery of feral swine involvement in the aetiology of a variety of pathogens, their propensity to transmit and carry a wide variety of pathogens is disconcerting. We examined sera from 2055 feral swine for antibody presence to six serovars of Leptospira that can also infect humans, livestock or domestic animals. About 13% of all samples tested positive for at least one serovar, suggesting that Leptospira infection is …

Higher Education: Opinions And Participation Among Nonmetropolitan Nebraskans: 2015 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy

Rural Futures Institute: Publications

Includes

Executive Summary

Introduction

Education or Training Taken

Participation in Education or Training Activities during Past Two Years

Types of Education or Training Taken in Past Two Years

Satisfaction with Types of Education or Training

Satisfaction with Types of Education or Training

Opinions about Education

Confocal Fluorescence Microscopy: An Ultra-Sensitive Tool Used To Evaluate Intracellular Antiretroviral Nano-Drug Delivery In Hela Cells, Subhra Mandal, You Zhou, Annemarie Shibata, Christopher J. Destache

School of Veterinary and Biomedical Sciences: Faculty Publications

In the last decade, confocal fluorescence microscopy has emerged as an ultra-sensitive tool for real-time study of nanoparticles (NPs) fate at the cellular-level. According to WHO 2007 report, Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) is still one of the world’s major health threats by claiming approximately 7,000 new infections daily worldwide. Although combination antiretroviral drugs (cARV) therapy has improved the life-expectancy of HIV-infected patients, routine use of high doses of cARVhas serious health consequences and requires complete adherence to the regimen for success. Thus, our research goal is to fabricate long-acting novel cARV loaded poly(lactide-co-glycolic acid) (PLGA) nanoparticles (cARV-NPs) as …

Characterization Of Cd8+ T Cell Differentiation Following Sivδnef Vaccination By Transcription Factor Expression Profiling, James M. Billingsley, Premeela A. Rajakumar, Michelle A. Connole, Nadine C. Salisch, Sama Adnan, Yury V. Kuzmichev, Henoch S. Hong, R. Keith Reeves, Hyung-Joo Kang, Wenjun Li, Qingsheng Li, Ashley T. Haase, R. Paul Johnson

Nebraska Center for Virology: Faculty Publications

The onset of protective immunity against pathogenic SIV challenge in SIVΔnef-vaccinated macaques is delayed for 15-20 weeks, a process that is related to qualitative changes in CD8⁺ T cell responses induced by SIVΔnef. As a novel approach to characterize cell differentiation following vaccination, we used multi-target qPCR to measure transcription factor expression in naïve and memory subsets of CD8+⁺ T cells, and in SIV-specific CD8⁺ T cells obtained from SIVΔnef-vaccinated or wild type SIVmac239-infected macaques. Unsupervised clustering of expression profiles organized naïve and memory CD8⁺ T cells into groups concordant with cell surface phenotype. Transcription factor …

Escherichia Coli Surface Display Of Single-Chain Antibody Vrc01 Against Hiv-1 Infection, Lin-Xu Wang, Michael Mellon, Dane Bowder, Meghan Quinn, Danielle Shea, Charles Wood, Shi-Hua Xiang

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to prevent HIV-1 infection. The VRC01 antibody mimics CD4-binding to gp120 and has broadly neutralization activities against HIV-1. We have designed a construct that can express the fusion peptide of the scFv-VRC01 antibody together with the autotransporter β-barrel domain of IgAP gene from …

Evidence For Placental Hpv Infection In Both Hiv Positive And Negative Women, Chrispin Chisanga, Dawn Eggert, Charles D. Mitchell, Charles Wood, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Human papillomaviruses (HPVs) have previously been reported to infect epithelial trophoblast cells of the placenta. To investigate this possibility, 200 placental samples from Zambian women were separated into HIV+ and HIV− groups and tested for HPV by redundant primer PCR, using GP5+/GP6+ and CPI/CPII primer sets. Three HPV genotypes (HPV6, 16 and 90) were detected in placental samples. Whereas, 20 different HPV genotypes were detected in vaginal sampling of the same patients, suggesting that compartment specific sub-populations of HPV may exist. The incidence of HPV16 in placental samples was almost 2-fold greater in HIV+ women compared to HIV− (p = …

The Hippo/Yap Pathway Interacts With Egfr Signaling And Hpv Oncoproteins To Regulate Cervical Cancer Progression, Chunbo He, Dagan Mao, Guohua Hua, Xiangmin Lv, Xingcheng Chen, Peter C. Angeletti, Jixin Dong, Steven W. Remmenga, Kerry J. Rodabaugh, Jin Zhou, Paul F. Lambert, Peixin Yang, John S. Davis, Cheng Wang

Nebraska Center for Virology: Faculty Publications

The Hippo signaling pathway controls organ size and tumorigenesis

through a kinase cascade that inactivates Yes-associated

protein (YAP). Here, we show that YAP plays a central role in

controlling the progression of cervical cancer. Our results suggest

that YAP expression is associated with a poor prognosis for cervical

cancer. TGF-α and amphiregulin (AREG), via EGFR, inhibit the Hippo

signaling pathway and activate YAP to induce cervical cancer cell

proliferation and migration. Activated YAP allows for up-regulation

of TGF-α, AREG, and EGFR, forming a positive signaling loop to

drive cervical cancer cell proliferation. HPV E6 protein, a major

etiological molecule of …

Observed And Expected Incidence Of Cervical Cancer In Lusaka And The Southern And Western Provinces Of Zambia, 2007 - 2012, Mulele Kalima, Kennedy Lishimpi, Jane L. Meza, Shinobu Watanabe-Galloway, Susan C. Msadabwe, Catherine K. Mwaba, Aaron L. Shibemba, Lewis Banda, Charles Wood, Robert M. Chamberlain, Amr S. Soliman

Nebraska Center for Virology: Faculty Publications

Objectives—Cervical cancer is increasing but underestimated in developing countries. We calculated the observed and expected incidence of cervical cancer in Lusaka and Southern and Western provinces of Zambia.

Methods/Materials—Data for 2007-2012 was obtained for the 3 provinces. Data included age, residence, year of diagnosis, marital status, occupation, HIV, stage, radiotherapy and chemotherapy. Expected incidence in Southern and Western provinces was calculated based on observed incidence for Lusaka province, adjusting for HIV.

Results—Crude and age-standardized incidence rates (ASR) in Lusaka were 2-4 times higher than incidence in the other 2 provinces. Lusaka had a rate of 54.1/10⁵ and ASR of …

Early Initiation Of Antiretroviral Therapy Can Functionally Control Productive Hiv-1 Infection In Humanized-Blt Mice, Qingsheng Li, For Yue Tso, Guobin Kang, Wuxun Lu, Yue Li, Wenjin Fan, Zhe Yuan, Christopher J. Destache, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background—Recent reports showed that functional control of HIV-1 infection for a prolonged time is possible by early anti-retroviral therapy (ART); however its underlying mechanism needs to be studied with a suitable animal model. Recently, humanized-BLT (bone marrow, liver and thymus) mouse (hu-BLT) was shown to be an excellent model for studying HIV-1 infection. We thus tested the feasibility of studying functional control of HIV-1 infection using hu-BLT mice.

Methods—Animals in three treatment groups (Rx-6h, Rx-24h, Rx-48h) and untreated group were infected with HIV-1, followed by ART initiation at 6, 24 or 48 hours post-infection and continued daily for two weeks. …

Activation And Degradation Of Open Reading Frame 45 By The Replication And Transcription Activator Of Kaposi's Sarcoma-Associated Herpesvirus, Ying Wang, Kai Yu, Xiuzhi Pei, Tiancheng Zhang, Yuying Guo, Charles Wood, Jinzhong Wang

Nebraska Center for Virology: Faculty Publications

The open reading frame 45 (ORF45) of the Kaposi's sarcoma-associated herpesvirus (KSHV) is an immediate-early phosphorylated tegument protein critical for viral escape from host immune surveillance. Its expression is upregulated by the viral replication and transcription activator (RTA), a key protein that controls the switch from latency to lytic replication. We report here that ORF45 expression was not only upregulated by RTA, but ORF45 could also be degraded by RTA in a proteasome-dependent manner. The ORF45 was activated by RTA via activation of the ORF45 promoter, and the promoter region from nt 69 271 to nt 69 026 was involved. …

Single-Dose Cpg Immunization Protects Against A Heterosubtypic Challenge And Generates Antigen-Specific Memory T Cells, Alexander J. Vogel, Deborah M. Brown

Nebraska Center for Virology: Faculty Publications

Despite extensive research, influenza A virus (IAV) remains a major cause of morbidity, mortality, and healthcare expenditure. Emerging pandemics from highly pathogenic IAV strains, such as H5N1 and pandemic H1N1, highlight the need for universal, crossprotective vaccines. Current vaccine formulations generate strain-specific neutralizing antibodies primarily against the outer coat proteins, hemagglutinin and neuraminidase. In contrast to these highly mutable proteins, internal proteins of IAV are more conserved and are a favorable target for developing vaccines that induce strong T cell responses in addition to humoral immunity. Here, we found that intranasal administration with a single dose of CpG and inactivated …

A Nuclear Fraction Of Turnip Crinkle Virus Capsid Protein Is Important For Elicitation Of The Host Resistance Response, Sung-Hwan Kang, Feng Qu, Thomas J. Morris

Nebraska Center for Virology: Faculty Publications

The N-terminal 25 amino acids (AAs) of turnip crinkle virus (TCV) capsid protein (CP) are recognized by the resistance protein HRT to trigger a hypersensitive response (HR) and systemic resistance to TCV infection. This same region of TCV CP also contains a motif that interacts with the transcription factor TIP, as well as a nuclear localization signal (NLS). However, it is not yet known whether nuclear localization of TCV CP is needed for the induction of HRT-mediated HR and resistance. Here we present new evidence suggesting a tight correlation between nuclear inclusions formed by CP and the manifestation of HR. …

A Novel Codon-Optimized Siv Gag-Pol Immunogen For Genebased Vaccination, Catherine M. Crosby, Eric A. Weaver, Reeti Khare, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Simian immunodeficiency virus (SIV) is a robust pathogen used in non-human primates to model HIV vaccines. SIV encodes a number of potential vaccine targets. By far the largest and most conserved protein target in SIV is its gag-pol protein that bears many epitopes to drive multivalent immune T cell responses. While gag-pol is an attractive antigen, it is only translated after a frame shift between gag and pol with the effect that gag and pol are expressed at an approximate 10/1 ratio. The codon bias of native lentiviral genes are also mismatched with the abundance of tRNAs in mammalian cells …

Differences In Prefrontal Cortex Activation And Deactivation During Strategic Episodic Verbal Memory Encoding In Mild Cognitive Impairment, Joana B. Balardin, Marcelo C. Batistuzzo, Maria Da Graca Moraes Martin, Joao R. Sato, Jerusa Smid, Claudia Porto, Cary R. Savage, Ricardo Nitrini, Edson Amaro Jr., Eliane C. Miotto

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

In this study we examined differences in fMRI activation and deactivation patterns during episodic verbal memory encoding between individuals with MCI (n = 18) and healthy controls (HCs) (n = 17). Participants were scanned in two different sessions during the application of self-initiated or directed instructions to apply semantic strategies at encoding of word lists. MCI participants showed reduced free recall scores when using self-initiated encoding strategies that were increased to baseline controls' level after directed instructions were provided. During directed strategic encoding, greater recruitment of frontoparietal regions was observed in both MCI and control groups; group differences …

The Need For Theory To Guide Concussion Research, Dennis Molfese

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

While the focus on concussion research has expanded greatly over the past decade, progress in identifying the mechanisms and consequences of head injury, the recovery path and the development of potential interventions to facilitate recovery have been largely absent. Instead, the field has largely progressed through an accumulation of data without the guidance of any systematic theory to guide the formulation of research questions or generate testable hypotheses. As part of this special issue on sports concussion, we advance a theory to describe the evolution of a neural network during the development of a cognitive process as well as the …

Resting-State Brain Connectivity After Surgical And Behavioral Weight Loss, Rebecca J. Lepping, Amanda S. Bruce, Alex Francisco, Hung-Wen Yeh, Laura E. Martin, Joshua N. Powell, Laura Hancock, Trisha M. Patrician, Florence J. Breslin, Niazy Selim, Joseph E. Donnelly, William M. Brooks, Cary R. Savage, W. Kyle Simmons, Jared M. Bruce

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Objective: Changes in food-cue neural reactivity associated with behavioral and surgical weight loss interventions have been reported. Resting functional connectivity represents tonic neural activity that may contribute to weight loss success. This study explores whether intervention type is associated with differences in functional connectivity after weight loss. Methods: Fifteen participants with obesity were recruited prior to adjustable gastric banding surgery. Thirteen demographically matched participants with obesity were selected from a separate behavioral diet intervention. Resting-state functional magnetic resonance imaging was collected 3 months after surgery/ behavioral intervention. ANOVA was used to examine post-weight loss differences between the two groups in …

Mineral Composition Of Serial Slaughter Holstein Carcasses, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter Walter, Nathan D. May, Jacob A. Reed, N. Andy Cole, Ty E. Lawrence, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Carcasses of 115 Holstein steers were divided into lean, bone, internal cavity, hide, and fat tissues for analysis of P, Ca, K, Mg, and S retention. Every 28 days, five steers from each of two treatments, fed Zilmax for 20 days prior to harvest or not fed Zilmax, were harvested. There were no differences due to treatment or days on feed when mineral retention was expressed as g/100 g of protein gain. Expressing mineral retention relative to protein gain reduced variation due to rate of gain and animal size.

Supplementing Cow-Calf Pairs Grazing Smooth Bromegrass, Jason M. Warner, Annie J. Doerr, Galen E. Erickson Erickson, Rick J. Rasby, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A three-year study evaluated supplementing ethanol co-products mixed with low-quality forage to cow-calf pairs grazing smooth bromegrass as a method to replace grazed forage intake. Supplementing a 30:70 modified distillers grains plus solubles:cornstalks mixture reduced estimated grazed forage intake by approximately 40%. Doubling the stocking rate and supplementing did not impact cow or calf performance. A summer supplementation program designed to reduce grazed forage intake is a viable strategy for increasing stocking rate if forage for grazing is limited.

Digestibility Of Calcium Oxide Treated Corn Residue With De-Oiled Distillers Grains, Sarah J. Peterson, Meredith L. Bremer, Adam L. Shreck Shreck, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A digestion study was conducted to evaluate diets containing calcium oxide treated corn residue in combination with de-oiled distillers grains in forage based growing diets. Chemical treatment did not affect digestibility of DM, OM, or NDF. However, concentration of distillers grains did improve DM and OM digestibility. The use of chemically treated residue in combination with distillers grains in growing diets may not impact diet digestibility.

Effects Of Dietary Fat Source And Monensin On Methane Emissions, Vfa Profile, And Performance Of Finishing Steers, Anna C. Pesta, Andrea K. Watson, Robert G. Bondurant Bondurant, Samodha C. Fernando, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study was conducted to evaluate the effects of dietary fat source and presence or absence of monensin on performance, methane (CH4) emissions, and ruminal VFA profile of cattle. No effects on performance or VFA profile were observed. Inclusion of modified distillers grain plus solubles (MDGS) in the diet tended to increase measures of CH4 production when compared to other fat sources (corn oil or tallow), while inclusion of monensin in the finishing diet was not significant for CH4 production.

Effects Of Grazing On Nebraska Sandhills Meadow Forage Nutrient Content, Jared V. Judy, Jacki A. Musgrave, L. Aaron Stalker Stalker, Karla H. Jenkins, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Nebraska Sandhills subirrigated meadow pastures were used to measure the effects of grazing on forage nutrient content in summer pastures. Non-grazed pastures had greater diet CP content than grazed pastures early in the grazing season. By late July, grazed vs. non-grazed pastures did not differ in diet CP content. Non-grazed pastures had greater in vitro organic matter disappearance compared with grazed pastures from late July through September; however, early summer pastures were not affected. Observed results indicate the greatest differences in nutrient content between grazed and non-grazed meadow pastures occur early and late in the grazing season when the majority …

Evaluation Of Changes In Nutritional Quality Of Corn Residue Over Time, Mandi Jones, James C. Macdonald, Terry J. Klopfenstein Klopfenstein, Galen E. Erickson, Keith Glewen, Andrea K. Watson

Nebraska Beef Cattle Reports

Irrigated corn residue was sampled across time in order to determine changes in quality and proportion of corn residue as the plant dried and was exposed to effects of weathering. Corn plants from two hybrids were planted on two different planting dates and harvested at periodic intervals from August 2012 to December 2012. Proportions of stem, blade/sheath, husk/shank, and cob made up smaller components of total plant DM as it matured, with the largest relative reduction occurring in the blade/sheath or stem. Hybrid impacted TDN values primarily because the 119 day hybrid was less mature at the early sampling dates.

Nutrient Digestibility And Ruminal Ph Of Finishing Diets Containing Dry Milling Byproducts With And Without Oil Extraction, Melissa L. Jolly-Breithaupt, Adam L. Shreck, Jana L. Harding Harding, James C. Macdonald, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

A metabolism trial was conducted to determine the effects of corn oil removal in condensed distillers solubles (CDS) and modified distillers grains plus solubles (MDGS) on nutrient digestibility and ruminal pH. Oil removal had no impact on DM, OM, or NDF digestibility in steers fed CDS or MDGS. However, steers fed de-oiled CDS had a lower fat digestibility than steers fed normal CDS. Average ruminal pH was lower for steers fed de-oiled MDGS than for steers fed normal MDGS, however no difference within CDS was observed.

Effect Of Distillers Grains Plus Solubles And Monensin Supplementation On Grazing Steers, Tyler L. Hasenauer, Terry J. Klopfenstein, James C. Macdonald Donald, Robby G. Bondurant, Dirk B. Burken

Nebraska Beef Cattle Reports

Yearling steers rotationally grazing smooth bromegrass were individually supplemented monensin at 0 or 200 mg with modified distillers grains plus solubles (MDGS) at .05, 0.4, 0.6, and 0.8% BW. Cannulated steers continuously grazing smooth bromegrass were assigned randomly to one of two treatments: 0.4% BW MDGS supplementation with 0 or 200 mg monensin. Monensin did not affect ADG of steers supplemented MDGS ≥ 0.4% BW. Steers supplemented with monensin had a decreasein estimated average forage intakefrom 16.16 lb to 14.75 lb/OM daily.

Using Enspira To Improve Fiber Digestion, Jana L. Harding, Adam L. Shreck, Melissa L. Jolly-Breithaupt Breithaupt, Galen E. Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A metabolism study was conducted to evaluate the effects of supplementing a fibrolytic enzyme (Enspira™) on total tract digestion of a finishing diet. In situ NDF digestibilities of the corn bran, HMC, corn residue, and corn silage were not different between the treatments. Rate of digestion of the corn residue and corn silage was lower for the enzyme treatment compared to the control. Averageruminal pH was not significantly different between the two treatments. Correspondingly, there was no difference in VFA profile. There were no differences in DM, OM, NDF, ADF, or hemicellulose digestibilities between the control and enzyme treatment.

Effects Of Replacing Corn With A Pelleted Treated Corn Stover And Distillers Grains On Performance Of Finishing Cattle, Jana L. Harding, Curtis J. Bittner, Dirk B. Burken Burken, Galen E. Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A finishing study evaluated the effectsof replacing 10, 20, or 30% corn (DM basis) with pelleted treated corn stover and distillers grains in a diet containing either 20 or 40% modified distillers grains plus solubles (MDGS) on finishing cattle performance. Steers consuming 10, 20, or 30% of the pelleted feed with 40% MDGS had equal or similar performance to the control diet with 40% MDGS. Cattle consuming 10% pelleted feed with 20% MDGS had similar efficiencies as the control diet; however, feeding the pellet at 20 or 30% of the diet DM with 20% MDGS decreased feed efficiency.

Feeding Value Of De-Oiled Wet Distillers Grains Plus Solubles Relative To Normal When Fed With Either Dry-Rolled Corn Or Steam-Flaked Corn In Beef Finishing Diets, Meredith L. Bremer, Marie E. Harris, Jake A. Hansen Hansen, Karla H. Jenkins, Matt K. Luebbe, Galen E. Erickson

Nebraska Beef Cattle Reports

A 128-day finishing study utilized 328 yearling steers to determine the effects of feeding de-oiled wet distillers grains plus solubles (WDGS) in dry rolled corn (DRC) or steam-flaked corn (SFC) diets relative to normal fat WDGS. No significant interactions were observed, but cattle fed DRC had greater DMI and were less efficient than those consuming SFC. Linear improvements in ADG and F:G were observed as concentration of de-oiled WDGS increased from 0 to 35%. Numerically cattle fed normal WDGS were more efficient than cattle fed de-oiled WDGS.

Response To Increasing Concentrations Of De-Oiled Modified Distillers Grains Plus Solubles In Beef Feedlot Diets, Meredith L. Bremer, Curtis J. Bittner, Dirk B. Burken Burken, Galen E. Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A 154-day finishing study, utilizing 378 calf-fed steers, was conducted to evaluate the response to feeding increasing concentrations of de-oiled modified distillers grains plus solubles (MDGS) on cattle performance and carcass characteristics. Two additional diets were fed to compare de-oiled MDGS to normal MDGS at either 15 or 30% inclusion. Increasing concentration of de-oiled MDGS in the diet resulted in a linear improvement in F:G. When comparing 30% de-oiled to normal MDGS, there was a tendency for 3.4% improvement in F:G for cattle fed normal MDGS diets over those fed de-oiled MDGS.