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Full-Text Articles in Medicine and Health Sciences

The Use Of Silver Diamine Fluoride In Dentistry In Virginia, Jessica Eisenberg Jan 2023

The Use Of Silver Diamine Fluoride In Dentistry In Virginia, Jessica Eisenberg

Theses and Dissertations

Purpose: The purpose of this research was to determine the use of silver diamine fluoride (SDF) in dentistry in Virginia and the factors that affect the practitioners’ decision. Methods: A survey was sent to the members of the Virginia Dental Association (VDA) by email and posted on the VDA social media accounts. The survey questions measured the practitioner’s demographics, knowledge, and factors that affect their utilization of SDF. Results: Most providers were general dentists (77%). The most common clinical situations for SDF use were to arrest caries in children with behavioral issues (77%), to delay restorative treatment (73%), in medically …


Cone Beam Computed Tomographic Analysis Of Outcomes In Ridge Augmentation Using Titanium Mesh And Titanium Reinforced Ptfe Membranes, Lina Elnakka Jan 2023

Cone Beam Computed Tomographic Analysis Of Outcomes In Ridge Augmentation Using Titanium Mesh And Titanium Reinforced Ptfe Membranes, Lina Elnakka

Theses and Dissertations

Objective: The primary goal of this study is to evaluate and compare the average vertical and horizontal bone gain between titanium reinforced polytetrafluoroethylene (Ti-PTFE) and titanium mesh (Ti-mesh) using superimposition of preoperative and postoperative (CBCT). The secondary goal is to evaluate and compare the surgical outcomes between the two techniques.

Methods: A retrospective chart review was completed to assess the clinical and radiographic outcomes of patients who underwent ridge augmentation therapy using either Ti-PTFE or Ti-mesh. Preoperative and postoperative CBCT scans were superimposed using InVivo 3D imaging software. Vertical component of the augmented site (L1) was assessed along a vertical …


Food Deserts And Pediatric Oral Health Status In An Under-Resourced Urban Community, Jessica T. Mcauliffe Jan 2023

Food Deserts And Pediatric Oral Health Status In An Under-Resourced Urban Community, Jessica T. Mcauliffe

Theses and Dissertations

Purpose: The aim of this analysis of survey data was to assess the influence of living within a food desert as a predictor of oral health problems among children of East Henrico County, Virginia.

Methods: Survey participants were recruited and questionnaires applied online. Residing in a food desert, using access to affordable produce as a proxy value, was the independent variable, while self- reported assessment of the child’s oral health status was the primary outcome variable. Analyses controlled for other indirect factors of pediatric oral health. Logistic regression was used to model the association between the self-reported access to healthy …


Motivations Of Adult Patients Seeking Orthodontic Retreatment, Scott V. Philips Jan 2023

Motivations Of Adult Patients Seeking Orthodontic Retreatment, Scott V. Philips

Theses and Dissertations

Objective: The aims of this study were (1) to compare the reasons adult patients seek orthodontic first-time treatment to the reasons adult patients seek orthodontic retreatment, and (2) to compare the concerns, motivations, and satisfaction levels of adult patients seeking orthodontic retreatment across the different previous treatment provider types of orthodontist, general dentist, and Direct-to-Consumer company.

Methods: At their initial orthodontic visit, adult patients (N=108) were invited to participate in the digital survey (REDCap) study. Subjects were recruited from the graduate orthodontics clinic at Virginia Commonwealth University and seven private orthodontic practices across the United States. Visual Analogue Scales were …


Studying Gene Function In Streptococcus Sanguinis Using Rna-Sequencing Analysis, Ahmed Ismail Jan 2023

Studying Gene Function In Streptococcus Sanguinis Using Rna-Sequencing Analysis, Ahmed Ismail

Theses and Dissertations

The oral cavity hosts hundreds of bacterial species, amongst them are the Streptococci family. More specifically, S. sanguinis is a benign bacteria in the oral cavity to protect the tooth surface from S. mutans. which is a pathogen causing dental caries. While S. sanguinis is harmless in the oral cavity, it becomes pathogenic once entering the bloodstream and causing Infective Endocarditis (IE). To further study S. sanguinis and eventually develop an effective drug to treat IE caused by S. sanguinis, a gene expression study was conducted. In order to study gene expression, RNA-sequencing was conducted, and appropriate methods to remove …


Impact Of Portal Of Exit On The Prevalence Of Apical Periodontitis In Root Canal Treated Maxillary Molars With Missed Second Mesiobuccal Canals: A Cbct Study, Jing Ye Jan 2023

Impact Of Portal Of Exit On The Prevalence Of Apical Periodontitis In Root Canal Treated Maxillary Molars With Missed Second Mesiobuccal Canals: A Cbct Study, Jing Ye

Theses and Dissertations

Objective: To evaluate the association of portal of exit (POE) of the second mesiobuccal (MB2) canal with the prevalence of apical periodontitis (AP) in previously treated maxillary molars

Methods: Cone beam computed tomography (CBCT) scans of 106 previously treated maxillary molars seen for either recall or retreatment in the past 3 years were included in the study. Scans were reviewed by one endodontic resident and one board-certified endodontist to determine the POE (separate versus common) of the missed MB2 canal and the presence or absence of a periapical hypo-dense area. Association between AP and portal of exit was assessed using …


Effect Of Apical Preparation Size On Endodontic Treatment Outcomes, Joseph Vaughn Jan 2023

Effect Of Apical Preparation Size On Endodontic Treatment Outcomes, Joseph Vaughn

Theses and Dissertations

Objective: Previous authors have attempted to identify the ideal apical preparation size needed to achieve endodontic treatment objectives. Despite vast literature on the topic, there exists a lack of consensus on ideal apical enlargement, with few studies discussing the effect of apical prep enlargement on clinical outcomes. The purpose of this retrospective study was to evaluate the effect of final apical preparation size on endodontic outcomes in maxillary and mandibular molars treated in both a graduate endodontic clinic and a private practice.

Methods: A chart review was conducted for patients seen at the VCU graduate endodontic clinic as well as …


Aadej Comm 9 1 2023 Jan 2023

Aadej Comm 9 1 2023

AADEJ - The Communicator

The Communicator is the official journal of the American Association of Dental Editors and Journalists (AADEJ). It is published quarterly, distributed digitally via email to members, and posted on the Association's website, AADEJ.org. Each issue includes regular updates from the editorial team and AADEJ board, as well as feature articles on current issues in the field.


Aadej Comm 9 3 2023 Jan 2023

Aadej Comm 9 3 2023

AADEJ - The Communicator

The Communicator is the official journal of the American Association of Dental Editors and Journalists (AADEJ). It is published quarterly, distributed digitally via email to members, and posted on the Association's website, AADEJ.org. Each issue includes regular updates from the editorial team and AADEJ board, as well as feature articles on current issues in the field.


Dental Claires: Contrastive Language Image Retrieval Search For Dental Research, Tanjida Kabir, Luyao Chen, Muhammad F Walji, Luca Giancardo, Xiaoqian Jiang, Shayan Shams Jan 2023

Dental Claires: Contrastive Language Image Retrieval Search For Dental Research, Tanjida Kabir, Luyao Chen, Muhammad F Walji, Luca Giancardo, Xiaoqian Jiang, Shayan Shams

Faculty, Staff and Student Publications

Learning about diagnostic features and related clinical information from dental radiographs is important for dental research. However, the lack of expert-annotated data and convenient search tools poses challenges. Our primary objective is to design a search tool that uses a user's query for oral-related research. The proposed framework,


Influences Of Race/Ethnicity In Periodontal Treatment Response And Bacterial Distribution, A Cohort Pilot Study, Bing-Yan Wang, Grayson Burgardt, Kavitha Parthasarathy, Daniel K Ho, Robin L Weltman, Gena D Tribble, Jianming Hong, Stanley Cron, Hua Xie Jan 2023

Influences Of Race/Ethnicity In Periodontal Treatment Response And Bacterial Distribution, A Cohort Pilot Study, Bing-Yan Wang, Grayson Burgardt, Kavitha Parthasarathy, Daniel K Ho, Robin L Weltman, Gena D Tribble, Jianming Hong, Stanley Cron, Hua Xie

Faculty, Staff and Student Publications

OBJECTIVES: Periodontitis disproportionately affects different racial and ethnic populations. We have previously reported the higher levels of

METHODS: This prospective cohort pilot study was carried out in an academic setting, at the School of Dentistry, University of Texas Health Science Center at Houston. Dental plaque was collected from a total of 75 African Americans, Caucasians and Hispanics periodontitis patients in a 3-year period. Quantitation of

RESULTS: The gains in clinical attachment levels after treatment significantly differed amongst the 3 groups-Caucasians responded most favorably, followed by African-Americans, lastly Hispanics, while numbers of

CONCLUSION: Differential response to nonsurgical periodontal treatment and distribution …


Identification Of Microbiological Factors Associated With Periodontal Health Disparities, Bing-Yan Wang, Aize Cao, Meng-Hsuan Ho, Derek Wilus, Sally Sheng, Hsiu-Wan Meng, Elissa Guerra, Jianming Hong, Hua Xie Jan 2023

Identification Of Microbiological Factors Associated With Periodontal Health Disparities, Bing-Yan Wang, Aize Cao, Meng-Hsuan Ho, Derek Wilus, Sally Sheng, Hsiu-Wan Meng, Elissa Guerra, Jianming Hong, Hua Xie

Faculty, Staff and Student Publications

The present study aimed at identifying risk factors associated with periodontitis development and periodontal health disparities with emphasis on differential oral microbiota. The prevalence of periodontitis is recently rising dentate adults in the US, which presents a challenge to oral health and overall health. The risk of developing periodontitis is higher in African Americans (AAs), and Hispanic Americans (HAs) than in Caucasian Americans (CAs). To identify potentially microbiological determinations of periodontal health disparities, we examined the distribution of several potentially beneficial and pathogenic bacteria in the oral cavities of AA, CA, and HA study participants. Dental plaque samples from 340 …


Editorial: The Impact Of Inflammation On The Oral Microbiome, Chun-Teh Lee, Loreto Abusleme, Thomas E Van Dyke Jan 2023

Editorial: The Impact Of Inflammation On The Oral Microbiome, Chun-Teh Lee, Loreto Abusleme, Thomas E Van Dyke

Faculty, Staff and Student Publications

No abstract provided.


Peripheral Calcifying Odontogenic Cyst: A Case Report And Comprehensive Review Of 60 Years Of Literature, Sally Sheng, Nicholas Tipton, Jennifer Chang, Hsiu-Wan Meng, Gena D Tribble Jan 2023

Peripheral Calcifying Odontogenic Cyst: A Case Report And Comprehensive Review Of 60 Years Of Literature, Sally Sheng, Nicholas Tipton, Jennifer Chang, Hsiu-Wan Meng, Gena D Tribble

Faculty, Staff and Student Publications

Peripheral Calcifying Odontogenic Cyst (PCOC) is the extraosseous form of calcifying odontogenic cyst that is limited to peripheral soft tissue without bony involvement. This case report presents a case of PCOC manifested as a progressive growth of gingival mass in a young male treated with excisional biopsy. Histological examination confirmed diagnosis of PCOC with presence of characteristic ghost cells and sporadic calcifications. No recurrence of the lesion and no complication were noted at three-year follow-up. Review of available literature on PCOC noted a predilection of occurrence in the mandible (61%) and in the anterior area of the jaws (58%). Mean …


Comparison Of Crown Fracture And Wear Resistence Of Resin 3d Printed Permanent Crown And Other Restorative Materials, Victor Moncada Jan 2023

Comparison Of Crown Fracture And Wear Resistence Of Resin 3d Printed Permanent Crown And Other Restorative Materials, Victor Moncada

All ETDs from UAB

Statement of Problem: The resin for 3D printing permanent crowns is a recently approved material with limited literature evidence to support its in vitro properties. Comparing the mechanical properties such as fracture load (N) and wear resistance for 3D printing permanent restorations resins would represent a very good reference to consider future clinical decisions. Purpose: To investigate the fracture load (N) and wear resistance of the resins for 3D printing permanent crowns available in the U.S. dental market and to compare amongst them using lithium disilicate as a reference. Materials and Methods: for the crown fracture test, the experimental design …


A Prospective Randomized Clinical Trial To Compare Side Access Mucosal Releasing Incision (Samri) And Sulcular Tunnel Access And Root Coverage Procedures With Coronally Advanced Flap And Acellular Dermal Matrix (Adm) To Treat Teeth With Gingival Recession And Lack Of Adequate Keratinized Tissue. A Pilot Study, Wangsoo Lee Jan 2023

A Prospective Randomized Clinical Trial To Compare Side Access Mucosal Releasing Incision (Samri) And Sulcular Tunnel Access And Root Coverage Procedures With Coronally Advanced Flap And Acellular Dermal Matrix (Adm) To Treat Teeth With Gingival Recession And Lack Of Adequate Keratinized Tissue. A Pilot Study, Wangsoo Lee

All ETDs from UAB

Aims. The aim of this prospective, randomized, blinded, controlled clinical trial was to compare clinical and patient-centered outcomes of Cairo recession type 1 (RT1) gingival recession defects treated with acellular dermal matrix (ADM) grafts and either Side Access Mucosal Releasing Incision (SAMRI) or Sulcular Tunnel Access (STA) techniques with coronal flap advancement (CAF). Methods. 11 patients, 22 surgical procedures and 81 gingival recession defect sites with Cairo RT1 gingival recession and £ 2mm of keratinized tissue at each site were randomly divided into two groups (A: SAMRI, B: STA) and were treated and evaluated for 12 months post-surgically. Clinical measurements …


Failure Modes Of Different Zirconia Crown Heights And Materials On Titanium Base Abutments, Aikaterini Anastasaki Jan 2023

Failure Modes Of Different Zirconia Crown Heights And Materials On Titanium Base Abutments, Aikaterini Anastasaki

All ETDs from UAB

Statement of the Problem: The existing scientific literature lacks comprehensive information regarding the influence of zirconia crown height on debonding and fracture occurrences between zirconia crowns and titanium base abutments. Additionally, there is a lack of comparative studies evaluating different zirconia materials as restorative options for screw-retained restorations. Purpose: The purpose of this study was to assess the failure modes of the zirconia crown/titanium implant abutment complex by investigating the impact of increasing zirconia crown heights and different zirconia materials. Materials and Methods: The experimental design encompassed six groups, each consisting of 10 specimens. Group 1 included 10 non-anatomical crowns …


Role Of Dpy30 In The Epigenetic Regulation Of Odontoblast Differentiation In Vitro, Namrata Landge Rana Jan 2023

Role Of Dpy30 In The Epigenetic Regulation Of Odontoblast Differentiation In Vitro, Namrata Landge Rana

All ETDs from UAB

Purpose: Dentin is produced from odontoblasts, derived from human Dental Pulp Cells (hDPCs), which are essential for dentin formation, remodeling, and mineral hemostasis. Epigenetic mechanisms are believed to be involved in odontoblast differentiation and function, and H3K4 methylation is associated with gene activation which is fundamental to histone-based epigenetics. The SET1/MLL complexes are mammals' major histone H3K4 methylation enzymes. DPY30 is a core subunit of SET1/MII complexes, mammalian's major histone H3K4 methyltransferases. It plays an important role in regulating fundamental processes including the determination of cell lineage, growth, and differentiation. Thus, histone-based epigenetic mechanisms are involved in odontoblast differentiation and …


Comparing Volumetric Wear Of Lithium Disilicate Against Different Ceramics And Enamel Antagonist An In-Vitro Study, Krisha Shah Jan 2023

Comparing Volumetric Wear Of Lithium Disilicate Against Different Ceramics And Enamel Antagonist An In-Vitro Study, Krisha Shah

All ETDs from UAB

Background: In patients with restored dentitions, it is possible for crowns on opposing teeth to gradually wear each other through chewing and bruxism. Therefore, the wear of restorative materials used for dental crowns is an important mechanical property for their long-term clinical success. Lithium disilicate is a widely used dental ceramic due to its strength, machinability, and translucency. Due to the rising use of ceramic restorations, a comparison of the wear resistance of lithium disilicate against different ceramic crown materials is useful for the clinician when selecting a crown material to oppose an existing lithium disilicate crown. Objective: To compare …


Effect Of Different Supporting Die Materials And Crown Thicknesses On The Fracture Load Of Monolithic 3y-Tzp Zirconia And Lithium Disilicate Crowns, Akram Mohamed Mohamed Gad Sayed Ahmed Jan 2023

Effect Of Different Supporting Die Materials And Crown Thicknesses On The Fracture Load Of Monolithic 3y-Tzp Zirconia And Lithium Disilicate Crowns, Akram Mohamed Mohamed Gad Sayed Ahmed

All ETDs from UAB

Background: Conventionally, the strength of dental materials used for fixed restorations is measured using specimens of simplified geometry (i.e bars or discs). There is value in measuring the strength of these materials in their anatomic geometry (i.e crowns) under clinically relevant loading conditions. One advantage of performing the so-called crown fracture test is that the material may benefit from its ability to be strengthened by its bond to a tooth-mimicking die. When performing a crown fracture test, the ideal die material is natural tooth structure. However, the variability in natural tooth anatomy and microstructure makes the use of a standardized, …


Short Root Anomaly: Prevalence, Genetics, And Its Effect In Orthodontic Treatment, Lauren Sharp Jan 2023

Short Root Anomaly: Prevalence, Genetics, And Its Effect In Orthodontic Treatment, Lauren Sharp

All ETDs from UAB

Short root anomaly (SRA) is a poorly understood developmental defect of root formation. Patients with this condition have short roots with rounded apexes and significantly reduced root to crown (R/C) ratios1, which can compromise dental care and may increase the risk of root resorption during tooth movement. SRA prevalence ranges from 1-10% but has not been studied in many ethnic populations. Furthermore, its genetic basis is undetermined. Our goals were to determine the prevalence of SRA among various ethnicities, identify genetic variations contributing to its phenotype, and evaluate how SRA can affect the outcome of orthodontic treatment. This study found …


Nasal Septum Deviation In Patients With Or Without Impacted Canines, Naurine Shah Jan 2023

Nasal Septum Deviation In Patients With Or Without Impacted Canines, Naurine Shah

All ETDs from UAB

There has been a recent rise in interest for studying the relationship between orthodontic treatment and the airway. Most studies have focused on measuring the oropharyngeal airway and how it is affected by growth with or without orthodontic treatment. The Nasal Septum, a midline structure is an integral part of the anatomy of the nasopharynx. Nasal Septum Deviations (NSD) can be caused by mechanical injuries, nasal polyps, neoplasia, infections, genetic influences as well as congenital malformations. The proximity of the septum can be correlated to changes in the housing of dentition in the naso maxillary complex. Objective: We aim to …


Effects Of Light Curing Bonding Agent With Dual Cure Cement On The Retention Of Zirconia And Lithium Disilicate Crowns: An In-Vitro Study., Mariam Issa Jan 2023

Effects Of Light Curing Bonding Agent With Dual Cure Cement On The Retention Of Zirconia And Lithium Disilicate Crowns: An In-Vitro Study., Mariam Issa

All ETDs from UAB

Objective: To measure and compare the bond strength of zirconia and lithium disilicate crowns bonded to human teeth (enamel and dentin) using universal adhesive with resin cement and different light curing protocols.Methods: Sixty extracted sound non carious human teeth (second premolars) were stored in formalternate solution (FormalternateTM, Concentrate, 500 mL, Canada) and the roots were notched and mounted into acrylic resin (Yates Motloid, USA). Flat surface preparation was done on the occlusal surface of the samples using model trimmer. The surface area of the specimens was measured with digital light microscopy. Samples were randomly divided into six groups of 10 …


Lateral Ridge Augmentation Using Allograft Bone Particles Hydrated With Or Without Recombinant Human Platelet-Derived Growth Factor: A Clinical And Histological Randomized Controlled Trial, Lauren Miele Jan 2023

Lateral Ridge Augmentation Using Allograft Bone Particles Hydrated With Or Without Recombinant Human Platelet-Derived Growth Factor: A Clinical And Histological Randomized Controlled Trial, Lauren Miele

All ETDs from UAB

Lateral ridge augmentation is a common procedure to increase the width of bone in preparation for a dental implant. A certain thickness of bone is necessary for implant health, making ridge augmentation procedures necessary for many patients. Research into the use of growth factors in ridge augmentation is ongoing, but few studies have examined the use of recombinant human platelet-derived growth factor homodimer (rhPDGF-BB) in ridge augmentation. This study examines histologic, clinical, and patient reported outcomes when horizontal ridge augmentation is completed using freeze dried bone allograft hydrated in rhPDGF-BB or saline and a resorbable crosslinked collagen membrane. Histologic results …


Functional Analysis Of Mandibular Movements In Three Different Groups Including Patients With Total Joint Replacements, Previous Orthognathic Surgery, And A Control Group, Adriana Morales Buendia Jan 2023

Functional Analysis Of Mandibular Movements In Three Different Groups Including Patients With Total Joint Replacements, Previous Orthognathic Surgery, And A Control Group, Adriana Morales Buendia

All ETDs from UAB

Purpose: To Assess mandibular movements, including mouth opening, lateral excursion, and protrusion among patients with total joint replacements and orthognathic surgery compared to healthy control participants with no known TMJ disorders. Methods: Fifty-two subjects from 18 to 66 years of age were included in this cross-sectional study, distributed in three groups: patients with total joint replacement (n = 16), patients who underwent orthognathic surgery (n=17), and healthy control subjects (n = 19) to quantitatively determine whether there is statistically significant difference among the groups regarding opening, lateral excursion, and protrusion movements. The non-invasive system SiCAT JMT+ device was used to …


Accuracy Of "Reading" Untraced Lateral Cephalometric Radiographs Among Practicing Orthodontists, Shannon Rea Jan 2023

Accuracy Of "Reading" Untraced Lateral Cephalometric Radiographs Among Practicing Orthodontists, Shannon Rea

All ETDs from UAB

Objective: To determine if orthodontic clinicians are able to ascertain diagnostic information from untraced lateral cephalometric radiographs. Materials and Methods: A survey consisting of 5 cases was presented to practicing orthodontists. Each case presented a lateral cephalometric radiograph and included ten questions requiring the respondent to make clinical judgments and approximations of the following variables: skeletal classification, maxilla and mandibular skeletal relationship, SNA, SNB, ANB, vertical skeletal classification, FMA, molar relationship, maxillary incisor proclination, and mandibular incisor proclination. 46 responses were collected and analyzed. Results: Case 3, the normodivergent, Skeletal Class I patient had the highest percentage of correct answers …


Comparison Of Flexural Strength, Fracture Toughness, And Color Stability Of Cad/Cam 3d-Printed Denture Base Materials To Milled And Conventional Heat-Polymerized Materials: An In Vitro Study, Yamen Safadi Jan 2023

Comparison Of Flexural Strength, Fracture Toughness, And Color Stability Of Cad/Cam 3d-Printed Denture Base Materials To Milled And Conventional Heat-Polymerized Materials: An In Vitro Study, Yamen Safadi

All ETDs from UAB

Digitalization of the design and manufacturing of complete dentures streamlines the clinical and laboratory processes and provides increased efficiency and enhanced denture quality.[1, 2] The two most common CAD/CAM techniques for denture base manufacturing are additive (3D-printing) and subtractive (milling) manufacturing. Milled denture bases have been proven to be comparable in their physical and mechanical properties to conventional heat-polymerized denture bases,[3-6] and in many studies found to be superior to 3D-printed denture bases.[7-13] However, 3D-printing offers many advantages over milling that appeal to both clinicians and dental technicians related to investment and operational costs, maintenance, speed of manufacturing, diversity of …


Comparison Of Radiological Signs To Clinical Symptoms In Children With Jia. A Pilot Study, Shubham Sharma Jan 2023

Comparison Of Radiological Signs To Clinical Symptoms In Children With Jia. A Pilot Study, Shubham Sharma

All ETDs from UAB

JIA, which stands for juvenile idiopathic arthritis, is a group of chronic inflammatory joint diseases that usually occur in children under the age of 16 and last for at least six weeks1–5. It is one of the most common chronic joint diseases in childhood1 and affects up to 150 out of every 100,000 children worldwide6. This condition has no identifiable cause and is different from adult rheumatoid arthritis. Unfortunately, JIA can have a significant negative impact on the lives of these young children, causing disability and impairing their daily activities. TMJ involvement is more common than previously believed in JIA …


Dentists’ Perspective On Using Text And Video Consultation To Improve Self-Study For The Modified Complete Denture Technique, Nutnicha Punyakornwong Jan 2023

Dentists’ Perspective On Using Text And Video Consultation To Improve Self-Study For The Modified Complete Denture Technique, Nutnicha Punyakornwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Complete edentulism has an adverse effect on the health and quality of life of older adults. However, patients frequently waited for years to receive treatment by prosthodontists. Simplified method could enable general dentists to treat edentulism efficiently. Tele-dentistry provides an approach for coaching general dentists in remote regions. In order to assist general dentists practicing the modified complete denture method, this study evaluated the learning outcomes of non-interactive and interactive self-study that used text and videoconferences. Participants were general dentists who had fewer than five years of experience. They were divided into non-interactive or interactive self-study group and received a …


Effect Of Simvastatin On Stem Cell Isolated From Apical Papilla (Scap) In Vitro, Paak Rewthamrongsris Jan 2023

Effect Of Simvastatin On Stem Cell Isolated From Apical Papilla (Scap) In Vitro, Paak Rewthamrongsris

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background and Objective: Simvastatin, known for lipid-lowering, shows promise in modulating stem cell behavior. This research investigates simvastatin's impact on apical papilla stem cells (SCAPs) in vitro. Methods: SCAPs were isolated, characterized, and exposed to varying simvastatin doses. Viability and proliferation were assessed with LIVE/DEAD and MTT assays. Cell cycle and apoptosis were examined via flow cytometry, and migration capacity was evaluated through scratch tests. Alizarin Red S staining and qPCR were used for odonto/osteogenic differentiation analysis. Results: Simvastatin reduced SCAP proliferation on days 3 and 7 and decreased colony size and density. Enhanced apoptosis and increased G0 subpopulation were …