Medicine and Health Sciences Commons^™

Chemotherapeutic Drug Resistance Associated With Differential Mirna Expression Of Mir-375 And Mir-27 Among Oral Cancer Cell Lines, Kieran Huni

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objective: Although progress has been made towards the identification of molecular profiles that influence oral cancer growth and metastasis, recent advances have suggested that non-coding microRNAs (such as miR-21, miR-27, miR-145, miR-155, miR-365, miR-375 and miR-494) may be involved in multiple aspects of chemotherapeutic responsiveness. Based upon the paucity of information regarding this phenomenon, this project will determine whether these specific microRNAs are correlated with oral cancer responsiveness to chemotherapies, including Paclitaxel, Cisplatin and Fluorouracil (5FU).Methods: Commercially available and well-characterized oral squamous cell carcinoma cell lines (SCC4, SCC9, SCC15, SCC25 and CAL27) were used for this study. Experimental 96-well assays …

Investigating The Influence Of Periodontal Disease On The Association Between Complex Multimorbidity And Health: A Cross-Sectional Study, Hussam M. Alqahtani, Siran M. Koroukian, Kurt C. Stange, Nicholas K. Schiltz, Nabil F. Bissada

Faculty Scholarship

Aims and Objectives: The aim of this study was to evaluate whether periodontal disease (PD) moderates the association between complex multimorbidity (MM) and fair/poor general health in a US representative sample of older individuals. Materials and Methods: This study identified 937 participants who were at least 60 years of age from the 2013-2014 National Health and Nutrition Examination Survey. A multivariable logistic regression model was used to estimate the association between complex MM (including chronic conditions, functional limitations, and geriatric syndromes) and self-reported fair/poor general health. Subsequently, we specified interactions between complex MM and PD to determine whether PD moderates …

An In-Vitro Analysis Of The Accuracy Of Different Guided Surgery Systems – They Are Not All The Same, Arndt Guentsch, Jennifer Bjork, Reagan Saxe, Shengtong Han, Andrew R. Dentino

School of Dentistry Faculty Research and Publications

Objectives

Different static computer-assisted implant surgery (sCAIS) systems are available that are based on different design concepts. The objective was to assess seven different systems in a controlled environment.

Materials and Methods

Each n = 20 implants were placed in identical mandible replicas (total n = 140). The systems utilized either drill-handles (group S and B), drill-body guidance (group Z and C), had the key attached to the drill (group D and V), or combined different design concepts (group N). The achieved final implant position was digitized utilizing cone-beam tomography and compared with the planned position. The angular deviation was …

10-Minute Ebd: Do Posterior Composite Restorations Have Longer-Term Success Than Glass Ionomer?, Eleni E. Langas Dds, Mridula Manoj Dds, Kelsey Carreras-Simons Dds

The Journal of the Michigan Dental Association

This Ten Minute Evidence Based Dentistry Article provides an example of the implementation of the evidence based dentistry search process with trusted search engines, and identification of the best literature through critical appraisal to answer the clinical question on long-term success of posterior resin vs. posterior glass ionomer restorations.

Finding Calm Amidst Chaos, Lisa Knowles Dds

The Journal of the Michigan Dental Association

This commentary on mindfulness and wellness provides the practitioner with strategies to achieve peace amidst chaos.

Oral Manifestations Of Acute Lymphocytic Leukemia, Angela Yepes Dds, Ms, Mba, Juan Yepes Dds, Md, Mph, Ms, Drph, James E. Jones Dmd, Msd, Edd, Phd

The Journal of the Michigan Dental Association

Leukemia is the most prevalent malignancy of leukocytes and can occur in children and adults. Oral manifestations are part of the complexity of signs and symptoms in patients with leukemia and, in some patients, oral manifestations precede systemic manifestations. Oral signs of leukemia include spontaneous bleeding, gingival enlargement, and whiteness of the oral mucosa. Head and neck lymphadenopathy is common in patients with leukemia. Oral manifestations are the result of the direct invasion of immature cells in the oral cavity tissues or indirectly caused by the replacement of the normal blood cells for immature cells causing anemia, leucopenia, and thrombocytopenia. …

The Human Role In Artificial Intelligence, Christopher J. Smiley Dds

The Journal of the Michigan Dental Association

The success of AI requires human involvement to ensure that it is used safely and effectively. Transparency, collaboration, and standardization are necessary.

J Mich Dent Assoc May 2023

The Journal of the Michigan Dental Association

In this issue, the reader will find the following original content:

• A case study on recognizing the oral signs of Acute Lymphatic Leukemia
• A special pull-out section to assist clinicians in identifying and treating common oral lesions
• Advice on personal Wellness: "Finding Calm Amidst Chaos."
• State of Michigan C.E. Requirements for License Renewal of Dentists, Hygienists, and Registered Dental Assistants.
• A 10Minute EBD contrasting clinical success of composite restorations vs. glass ionomers.
• Guest commentary "Pretty Good Dentistry."
• News you need, Editorial, and an Advocacy Spotlight.

Aesthetic Management Of Peri-Implant Soft Tissue Dehiscence. A Case Report Of A Combined Perio Restorative Approach, Ahmed Hamdy, Dalia M. Ghalwash

Dentistry

This case report designates a combined perio restorative technique in the management of peri-implant 3-dimensional soft tissue defects in an esthetic zone. A staged approach was implemented to treat this case; the first stage was planned to treat the horizontal defect around the implant, in which crown was removed, and coronally advanced flap with connective tissue graft were used to treat the soft tissue defect. At the second stage, a modified coronally advanced flap with connective tissue graft was used to augment the vertical defect, and no vertical releasing incision was made in this stage to maximize the blood supply …

Do Nasal Anatomical Variations Affect The Maxillary Sinus? A Cbct Volumetric Analysis, Raghdaa A. Mostafa, Sahar M. Samir

Journal of Dentistry Indonesia

Objective: This study aimed to determine the effect of the concha bullosa (CB) and the nasal septum deviation (NSD) on the volume of the maxillary sinuses using ITK-SNAP (http://www.itksnap.org/ download/snap/). Methods: A total of 70 CBCT scans were analyzed. Three groups were evaluated; control (absence of CB), unilateral CB, and bilateral CB. Moreover, scans were classified according to the NSD into control (no deviation), mild, moderate, and severe NSD deviation. The volume of each maxillary sinus was calculated using the ITKSNAP. Intra-observer reliability analysis was performed by paired-sample t-test. Analysis of variance ANOVA and t-test were used to …

A Prospective-Randomized Study: The Impact Of Four Different Caries Removal Method On Pain And Clinical Evaluations, Merve Aydemir, Serdar Bağlar

Journal of Dentistry Indonesia

Objective: There are many different methods for removing caries. In this study, to evaluate four caries removal methods in terms of patient comfort and to evaluate the clinical success of restorations according to modified-USPHS criteria. Methods: In 31 patients with at least 4 Class II caries in their posterior teeth, 4 teeth were randomly divided into four groups and 4 different methods (conventional method, Carisolv, Papacarie, Er-Cr:YSGG Laser) were used for caries removal. Pain formation during caries removal was determined by FACE Pain Scale questionnaire. The restorations were controlled with Modified-USPHS criteria in 3-6-12 months period. Mann-Whitney U …

Assessment Of Dna Damage Of Oral Mucosa Due To Ceramic Bracket Using Comet Assay And Mutagenicity Of Orthodontic Bonding System Using Ames Test, Riaz Adham, Norma Ab Rahman, Kannan Thirumulu Ponnuraj

Journal of Dentistry Indonesia

Objective: The objective of this study was to determine the DNA damage caused by ceramic bracket (Clear II, SIA Orthodontic Manufacturer Srl, Italy) on human buccal mucosal cells using comet assay and the mutagenicity of orthodontic bonding system (Transbond XT; 3M Unitek) by Ames test. Methods: In this study, twenty orthodontic patients were recruited from Specialist Orthodontic Clinic, Hospital Universiti Sains Malaysia. The Buccal mucosal cell sample was obtained from each patient at three time points-before (TO), after one month (T1) and after two months (T2) of ceramic bracket placement for performing comet assay. The spot test version …

Students’ Experience, Self-Confidence, And Perception Toward Endodontic Learning: National Survey Among Malaysian Dental Schools, Siti Hajar Omar, Aspalilah Alias, Safura Anita Baharin

Journal of Dentistry Indonesia

Objective: This study aimed to evaluate the first endodontic case experiences, self-confidence levels, and perceptions toward endodontic learning of final-year undergraduate students. Methods: An online questionnaire was emailed to 270 final-year dental students at 10 Malaysian dental schools. The questionnaire consists of 29 questions on students’ experience, self-confidence, and perception toward endodontic learning. Results: The response rate was 74.4%. The most frequently treated case was tooth diagnosed as nonvital associated with periapical periodontitis. Most of the students perceived working length determination as the most difficult stage and reported having low confidence in locating canal orifices in multirooted …

Treatment Of Oral Vascular Malformations With Ethamolin: A Report Of Two Cases, Maria Clara De Moura Santos Coelho Ferreira, Marco Vinícius De Sales Lima, Anna Laura Almeida Barreto, Dárcio Kitakawa, Alexandre Do Prado Scherma, Felipe Peralta, Luis Felipe Das Chagas E Silva De Carvalho

Journal of Dentistry Indonesia

A hemangioma is a benign vascular neoplasm, relatively common in the head and neck. In the oral region, it affects the tongue, gums, mucosa, palate, and lip. Its size can vary from millimeters to centimeters, characterized as a purplish or reddish lesion. The present study aims to present two clinical cases of hemangiomas, the first of a male patient treated by sclerotherapy and the second of a female patient in which she was treated with ethamolin and later surgical treatment. It was concluded that sclerotherapy, in addition to being a safe treatment, managed to drastically reduce the initial size of …

A Two-Year Retrospective Evaluation Of Cone Beam Computed Tomography Indications In Pediatric, Adolescent And Adult Patients, Zeynep Betül Arslan, Esra Ceren Tuğutlu

Journal of Dentistry Indonesia

Objective: The objective was to evaluate cone-beam computed tomography (CBCT) indications and distribution in pediatric, adolescent and adult patients. Methods: A total of 1013 CBCT images were reviewed in detail from the electronic patient database. The patient’s age, gender, CBCT indications and acquisition sites, referral departments, reason for referral, incidental findings in the imaging field and the presence of artifacts in the CBCT images were all recorded. The European DIMITRA project recommendations were used to categorize CBCT indications in the children and the European Guidelines were used for adults. Results: From a total of 1013 images; 5.3% …

Treatment Of Gingival Fenestration Using Mucoperiosteal Flap With Connective Tissue Graft: A Report Of Two Cases, Swati Jaglan, Shikha Tewari

Journal of Dentistry Indonesia

Fenestration term is derived from the Latin word ‘fenestra’ means ‘window’. Gingival fenestration is not found in routine while its actual occurrence is assumed to be higher. Additionally, if these mucogingival defects persist for a longer duration it may lead to adverse outcomes like compromised esthetics, hypersensitivity, plaque and calculus deposition, and susceptibility to dental caries. Present cases of gingival fenestration were successfully treated by using full-thickness flap elevation with connective tissue graft placement. Two cases were presented with the accumulation of plaque and calculus and having gingival fenestration to the respective sites. After phase 1 therapy, full thickness flap …

Management Of Tooth Hypersensitivity Under A Cantilever Fixed Partial Denture Using A Novel Low-Level Laser Therapy Protocol: A Case Report, Mohamed Fattouh, Manal Mohammed Aljuaid, Nada Ali Abdelaleem

Journal of Dentistry Indonesia

Tooth hypersensitivity is a common clinical complaint after dental treatment.

Objective: This study presents a case report of the use of a diode laser for treating tooth hypersensitivity in a patient with a cantilever fixed partial denture abutment.

Case Report: A 23-year-old female patient presented to the Faculty of Dentistry, Umm Al-Qura University in Makkah, with the chief complaint of pain in a deeply carious maxillary right first premolar. The tooth was extracted, and a cantilever fixed partial denture was placed using the second premolar as an abutment. One year later, the patient experienced tooth hypersensitivity under the bridge. Low-level …

Head And Neck Tumor Histopathological Image Representation With Pre- Trained Convolutional Neural Network And Vision Transformer, Ranny Rahaningrum Herdiantoputri, Daisuke Komura, Tohru Ikeda, Shumpei Ishikawa

Journal of Dentistry Indonesia

Image representation via machine learning is an approach to quantitatively represent histopathological images of head and neck tumors for future applications of artificial intelligence-assisted pathological diagnosis systems. Objective: This study compares image representations produced by a pre-trained convolutional neural network (VGG16) to those produced by a vision transformer (ViT-L/14) in terms of the classification performance of head and neck tumors. Methods: W hole-slide images of five oral t umor categories (n = 319 cases) were analyzed. Image patches were created from manually annotated regions at 4096, 2048, and 1024 pixels and rescaled to 256 pixels. Image representations were …

Ramsay Hunt Syndrome With Oral Findings: A Rare Case, İlknur Eninanç, Büşra Şahin

Journal of Dentistry Indonesia

Ramsay Hunt syndrome (RHS) is a disease that is caused by the varicella-zoster virus and is characterized by severe ear pain, auricular vesicular eruptions, and peripheral facial paralysis.

Objective: The aim of this case report is to provide information about the clinical findings and treatment process of RHS, which is a rare case and may have oral findings and stress the importance of early diagnosis.

Case Report: A 60-year-old male patient had previously consulted an otolaryngologist and a family physician with complaints of vesicular eruptions in the right ear auricle and on the mandible. The patient in whom a diagnosis …

Dental Fear, Anxiety, And Phobia – Behavioral Management And Implications For Dentists, Nadya Tsetsova Avramova

Journal of Mind and Medical Sciences

Clinical management of patients with dental fear, anxiety, and phobia could be a major challenge to daily practice of dental professionals. The aim of the current review was to expand the modern consideration with respect to these issues, and therefore to discuss basic approaches and psychological techniques for management of oral health behaviors that should be employed in dentistry. This paper presents detailed description of the characteristics of dentist-patient therapeutic relationship as well as the most used techniques to cope with dental fear, anxiety, and phobia - relaxation techniques, cognitive-behavioral therapy (CBT), hypnosis and melotherapy. We can still argue that …

The Effect Of Fluorouracil On Pathogenic And Non-Pathogenic Oral Microorganisms And Microbial Interactions With Preventative Measures: A Preliminary Study, Valeria Ortiz Jimenez, Lynn Lewis

Departmental Honors & Graduate Capstone Projects

Cancer chemotherapy compromises the patient’s oral health through dysbiosis of oral microbiota and increases the prevalence of dental cavities, gingivitis, oral mucositis, and xerostomia. This research aimed to evaluate the effect of a common chemotherapeutic agent, Fluorouracil (5-FU), on certain microorganisms that are common within the oral cavity. Varying concentrations (50 μM, 75 μM and 100μM) of 5-FU were used to simulate the dosage that reaches the oral cavity after intravenous delivery. The microorganisms tested were Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Lactobacillus rhamnosus and Streptococcus salivarius. These are some of the most common ones found in the …

Bone Marrow Endosteal Stem Cells Dictate Active Osteogenesis And Aggressive Tumorigenesis, Yuki Matsushita, Jialin Liu, Angel Ka Yan Chu, Chiaki Tsutsumi-Arai, Mizuki Nagata, Yuki Arai, Wanida Ono, Kouhei Yamamoto, Thomas L Saunders, Joshua D Welch, Noriaki Ono

Faculty, Staff and Student Publications

The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. It is well-described that leptin receptor (LepR)+ perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. However, the identity of SSCs in young bone marrow and how they coordinate active bone formation remains unclear. Here we show that bone marrow endosteal SSCs are defined by fibroblast growth factor receptor 3 (Fgfr3) and osteoblast-chondrocyte transitional (OCT) identities with some characteristics of bone osteoblasts and chondrocytes. These Fgfr3-creER-marked endosteal stromal …

Evaluation Of Burning Mouth Syndrome And Salivary Chromogranin A In Major Depressive Disorder Patients, Ameer Ali Althabhawee, Taghreed Fadhil Zaidan

Maaen Journal for Medical Sciences

Major depressive disorder represents a growing concern as its prevalence keeps rising worldwide; with this rising, there is also an increased demand in the field of oral medicine in developed countries, and since many disorders and orofacial pain have been linked to be associated with psychiatric disorders and conditions, this study was aimed to evaluate burning mouth syndrome and salivary chromogranin A in major depressive disorder patients. The study sample included 49 patients who received a diagnosis of major depressive disorder, being under treatment for at least two weeks. The control group consists of 34 healthy subjects with no signs …

Evaluation Of Heated Cure Acrylic Immersed In Ozonated Water, Shawq Azmi Tawfeeq, Aseel Mohammed Al-Khafaji

Maaen Journal for Medical Sciences

Background: Denture cleansing is an important step that can prevent the spread of infection and improve a patient's health, the durability of the dentures, and the overall quality of life; therefore, it is necessary to choose a suitable cleanser that, in addition to being effective, does not have an unfavorable effect on the qualities of the denture base resin itself when used for an extended period.

Aim: This research aims to assess the effects of ozonated water on the surface roughness and hardness of heat-cured acrylic resin by immersion technique.

Materials: Sixty bars shape made of heat-cured acrylic were manufactured. …

Special Needs In Pediatric Dentistry, Emma Kail

Health Sciences Presentations

Over the summer of 2022 I got the chance to work on my internship at a special needs and non special needs focused dentistry. Through this presentation I focus mainly on the pediatric special needs aspect of the dental care given to patients. Throughout the poster I speak about the importance of special needs care within dentistry and the field of dental health itself, where I worked and how they accommodated and worked with those with special needs to give the best care possible, and finally how this experience has changed my goals set for my future, especially within the …

Independent Effects Of Hiv And Antiretroviral Therapy On The Oral Microbiome Identified By Multivariate Analyses, Clifford J. Beall, Elizabeth A. Lilly, Carolina Granada, Kelly Treas, Kenneth R. Dubois, Shahr B. Hashmi, Jose A. Vazquez, Michael E. Hagensee, Ann L. Griffen, Eugene J. Leys, Paul L. Fidel

School of Dentistry Faculty Publications

The oral microbiome is an important predictor of health and disease. We recently reported significant yet modest effects of HIV under highly active antiretroviral therapy (ART) on the oral microbiome (bacterial and fungal) in a large cohort of HIV-positive (HIV+) and matched HIV-negative (HIV-) individuals. As it was unclear whether ART added to or masked further effects of HIV on the oral microbiome, the present study aimed to analyze the effects of HIV and ART independently, which also included HIV- subjects on preexposure prophylaxis (PrEP) therapy. Cross-sectional analyses of the effect of HIV devoid of ART (HIV+ ART- versus matched …

Oral And Dental Health Of College Students On A Private University Campus, David Abafi, Laura Frable

Liberty University Research Week

Graduate

Theoretical Proposal

Temporomandibular Joint Dysfunction (Tmjd), Jorge Pumachagua, Christopher Barnes, Elias Saliba

Discovery Day - Daytona Beach

The goal of this senior design project is to produce an alternative to the current market of dental guards available that non-invasively treat Temporomandibular joint dysfunction (TMJD). TMJD occurs when there is an anterior dislocation of the articular disk in reference to the condyle. The focus of the design is to prevent patients that have been rehabilitated by a specialist from regressing due to bruxism. Bruxism is a condition where teeth grind, gnash, or clench involuntarily at any time of the day, especially during sleep time. Bruxism engages the Masseter, Temporalis, Medial
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Class Iii Malocclusion Camouflage Treatment Using A Conventional Orthodontic Appliance In A Non-Growing Patient, Fransiska Monika, Retno Widayati

Journal of Dentistry Indonesia

The treatment of skeletal and dental Class III malocclusions is a challenge for orthodontists, and one of the treatment alternatives for a non-growing patient is orthodontic camouflage treatment. Different approaches may result in different outcomes; therefore, a proper diagnosis and treatment plan are needed to avoid undesirable effects. In this case, a 21-year-old female patient presented with a skeletal Class III malocclusion, with a normal maxilla, prognathic mandible, moderate crowding, and an acceptable facial profile. The treatment modality for orthodontic camouflage treatment was a conventional orthodontic appliance with the extraction of mandibular first premolars to resolve crowding. At the end …

Ada News - 04/10/2023, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.