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Full-Text Articles in Medicine and Health Sciences
Hydroxylamine Acutely Activates Glucose Uptake In L929 Fibroblast Cells, Larry L. Louters, Jared P. Scripture, David P. Kuipers, Stephen M. Gunnink
Hydroxylamine Acutely Activates Glucose Uptake In L929 Fibroblast Cells, Larry L. Louters, Jared P. Scripture, David P. Kuipers, Stephen M. Gunnink
University Faculty Publications and Creative Works
Nitroxyl (HNO) has a unique, but varied, set of biological properties including beneficial effects on cardiac contractility and stimulation of glucose uptake by GLUT1. These biological effects are largely initiated by HNO's reaction with cysteine residues of key proteins. The intracellular production of HNO has not yet been demonstrated, but the small molecule, hydroxylamine (HA), has been suggested as possible intracellular source. We examined the effects of this molecule on glucose uptake in L929 fibroblast cells. HA activates glucose uptake from 2 to 5-fold within two minutes. Prior treatment with thiol-active compounds, such as iodoacetamide (IA), cinnamaldehyde (CA), or phenylarsine …
Differential Regulation Of Glut1 Activity In Human Corneal Limbal Epithelial Cells And Fibroblasts, David P. Kuipers, Jared P. Scripture, Stephen M. Gunnink, Matthew J. Salie
Differential Regulation Of Glut1 Activity In Human Corneal Limbal Epithelial Cells And Fibroblasts, David P. Kuipers, Jared P. Scripture, Stephen M. Gunnink, Matthew J. Salie
University Faculty Publications and Creative Works
The corneal epithelial tissue is a layer of rapidly growing cells that are highly glycolytic and express GLUT1 as the major glucose transporter. It has been shown that GLUT1 in L929 fibroblast cells and other cell lines can be acutely activated by a variety agents. However, the acute regulation of glucose uptake in corneal cells has not been systematically investigated. Therefore, we examined glucose uptake in an immortalized human corneal-limbal epithelial (HCLE) cell line and compared it to glucose uptake in L929 fibroblast cells, a cell line where glucose uptake has been well characterized. We report that the expression of …