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- Ginseng replant disease (2)
- Soil (2)
- American ginseng (Panax quinquefolius) (1)
- Atmospheric nitrogen deposition (1)
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- Extraction (1)
- GRD (1)
- Genes (1)
- Ginseng (1)
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- Long-term study (1)
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- RNAseq (1)
- Sandy-loam (1)
- Soil chemistry (1)
- Species composition (1)
- Suberin (1)
- Temperate old-field (1)
- WGCNA (1)
- Warming addition (1)
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Articles 1 - 4 of 4
Full-Text Articles in Plant Sciences
Long-Term Vs. Short-Term Plant Responses To Warming And Nitrogen Addition In A Temperate Old Field, Breanna Craig
Long-Term Vs. Short-Term Plant Responses To Warming And Nitrogen Addition In A Temperate Old Field, Breanna Craig
Electronic Thesis and Dissertation Repository
Climate warming and atmospheric nitrogen (N) deposition are expected to have strong effects on plant productivity in temperate ecosystems over the next century. However, short-term experiments may not adequately address their potential effects because of lags in changes in species composition. I added new plots to a pre-existing field experiment to compare the short-term (1-2 year; new plots) vs. long-term (14-15 year; old plots) effects of warming and N addition on plant productivity, relative species abundances, plant tissue N content, and litter decomposition. In 2020, N addition increased aboveground plant productivity most in old plots and only increased belowground biomass …
Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo
Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo
Undergraduate Student Research Internships Conference
Ginseng is a high value perennial crop grown for its roots. A four-year cultivation cycle is required to obtain marketable ginseng roots, during which a condition called ginseng replant disease (GRD) develops. GRD is characterized by a severe root rot disease, and prevents the growth of newly planted ginseng in a former ginseng garden1. Microbiome analysis of ginseng garden soil will help our understanding of the formation of GRD and its severity. However, extracting DNA from the sandy-loam soils required for ginseng cultivation is difficult. Commercial kits are either too expensive or not applicable to the large volumes of soil …
Using Wgcna For Comprehensive Understanding Of Genes Involved In Suberin Biosynthesis From Rna-Seq Data Analysis, Stephanie Wu
Using Wgcna For Comprehensive Understanding Of Genes Involved In Suberin Biosynthesis From Rna-Seq Data Analysis, Stephanie Wu
Undergraduate Student Research Internships Conference
Suberin is a heteropolymer comprising a cell wall-bound poly(phenolic) domain (SPPD) covalently linked to a poly(aliphatic) domain (SPAD) that is deposited between the cell wall and plasma membrane (Woolfson 2018). Previous studies have measured phenolic and/or fatty acid compounds in suberized tissues, and for many of these the biosynthesis is known, However, restricting analyses to only those biomolecules that are known does not yield new information about other genes that may be involved. This experiment serves to identify suberin associated gene clusters within Modules, based on an analysis of their expression patterns using Weighted Gene Correlation Network Analysis (WGCNA). Once …
The Analysis Of Ginsenosides In Ginseng Garden Soil, Karina Marie Kaberi
The Analysis Of Ginsenosides In Ginseng Garden Soil, Karina Marie Kaberi
Electronic Thesis and Dissertation Repository
American ginseng (Panax quinquefolius L.) produces natural products called ginsenosides. The biggest challenge Ontario commercial ginseng farmers face is ginseng replant disease. To understand the function of ginseng root exudates, ginsenoside accumulation and persistence over time were investigated. Currently, no reliable ginsenoside specific extraction method, characterizing the changes in soil chemistry exists. Ginsenoside extraction protocol optimization was required to determine how ginsenoside composition changed over time. Overall, protocol optimization resulted in a 30% increase in yield of ginsenosides compared to previous extraction protocols. In the ginseng gardens, ginsenoside accumulation occurs slowly and did not reach significantly measurable amounts until the …