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Full-Text Articles in Plant Sciences
Chromosome-End Knockoff Strategy To Reshape Alkaloid Profiles Of A Fungal Endophyte, Simona Florea, Timothy D. Phillips, Daniel G. Panaccione, Mark L. Farman, Christopher L. Schardl
Chromosome-End Knockoff Strategy To Reshape Alkaloid Profiles Of A Fungal Endophyte, Simona Florea, Timothy D. Phillips, Daniel G. Panaccione, Mark L. Farman, Christopher L. Schardl
Faculty & Staff Scholarship
Molecular genetic techniques to precisely eliminate genes in asexual filamentous fungi require the introduction of a marker gene into the target genome. We developed a novel strategy to eliminate genes or gene clusters located in subterminal regions of chromosomes, and then eliminate the marker gene and vector backbone used in the transformation procedure. Because many toxin gene clusters are subterminal, this method is particularly suited to generating nontoxic fungal strains. We tested this technique on Epichloë coenophiala, a seed-transmissible symbiotic fungus (endophyte) of the important forage grass, tall fescue (Lolium arundinaceum). The endophyte is necessary for maximal productivity and sustainability …
Data Supporting The Nuclear Phylogenomics Of The Palm Subfamily Arecoideae (Arecaceae), Jason R. Comer, Wendy B. Zomlefer, Craig F. Barrett, Dennis Wm. Stevenson, Karolina Heyduk, James H. Leebens-Mack
Data Supporting The Nuclear Phylogenomics Of The Palm Subfamily Arecoideae (Arecaceae), Jason R. Comer, Wendy B. Zomlefer, Craig F. Barrett, Dennis Wm. Stevenson, Karolina Heyduk, James H. Leebens-Mack
Faculty & Staff Scholarship
This data article provides data and supplemental materials refer-enced in“Nuclear phylogenomics of the palm subfamily Arecoi-deae (Arecaceae)”(Comer et al., 2016)[1]. Raw sequence readsgenerated for this study are available through the Sequence ReadArchive (SRA Study Accession: SRP061467). An aligned super-matrix of 168 nuclear genes for 35 taxa (34 palms and one out-group taxon) is provided. Also provided are individual maximumlikelihood gene trees used for the coalescent based analyses, out-put from the maximum parsimony analyses, and twofigures.
Transcriptome And Metabolome Analyses Of Glucosinolates In Two Broccoli Cultivars Following Jasmonate Treatment For The Induction Of Glucosinolate Defense To Trichoplusia Ni (Hübner), Kang-Mo Ku, Talon M. Becker, John A. Juvik
Transcriptome And Metabolome Analyses Of Glucosinolates In Two Broccoli Cultivars Following Jasmonate Treatment For The Induction Of Glucosinolate Defense To Trichoplusia Ni (Hübner), Kang-Mo Ku, Talon M. Becker, John A. Juvik
Faculty & Staff Scholarship
Lepidopteran larvae growth is influenced by host plant glucosinolate (GS) concentrations, which are, in turn, influenced by the phytohormone jasmonate (JA). In order to elucidate insect resistance biomarkers to lepidopteran pests, transcriptome and metabolome analyses following JA treatments were conducted with two broccoli cultivars, Green Magic and VI-158, which have differentially induced indole GSs, neoglucobrassicin and glucobrassicin, respectively. To test these two inducible GSs on growth of cabbage looper (Trichoplusia ni), eight neonate cabbage looper larvae were placed onto each of three plants per JA treatments (0, 100, 200, 400 M) three days after treatment. After five days of feeding, …