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Characterization Of Bacteriophages Of Pseudomonas Syringae Pv. Tomato, Sara E. Prior, Amanda J. Andrews, Russell O. Nordeen

Journal of the Arkansas Academy of Science

Bacteriophages from supernatants of the plant pathogenic bacteria Pseudomonas syringae pv. tomato (P. tomato) were isolated, enriched, and purified by density block centrifugation in cesium chloride (CsCl) step gradients. The DNA from purified phage was isolated and digested with the restriction endonucleases EcoRl or HindIII. Three different DNA fingerprint patterns were determined indicating 3 unique phage isolates. Genome sizes of the phage ranged from 40 to 52 kilobases (kB). Buoyant densities of phage particles in CsCI varied from 1.36 to 1.51 glml. Electron microscopy revealed a single morphological type with an elongated polyhedral head and a long tail indicating the …