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Full-Text Articles in Plant Sciences
Ether Bridge Formation And Chemical Diversification In Loline Alkaloid Biosynthesis, Juan Pan
Ether Bridge Formation And Chemical Diversification In Loline Alkaloid Biosynthesis, Juan Pan
Theses and Dissertations--Plant Pathology
Loline alkaloids, found in many grass-Epichloë symbiota, are toxic or feeding deterrent to invertebrates. The loline alkaloids all share a saturated pyrrolizidine ring with a 1-amine group and an ether bridge linking C2 and C7. The steps in biosynthesis of loline alkaloids are catalyzed by enzymes encoded by a gene cluster, designated LOL, in the Epichloë genome. This dissertation addresses the enzymatic, genetic and evolutionary basis for diversification of these alkaloids, focusing on ether bridge formation and the subsequent modifications of the 1-amine to form different loline alkaloids.
Through gene complementation of a natural lolO mutant and comparison …
Boron Nutrition Of Burley And Dark Tobacco, Laura Ann Frakes Mitchell
Boron Nutrition Of Burley And Dark Tobacco, Laura Ann Frakes Mitchell
Theses and Dissertations--Plant and Soil Sciences
The incidences of suspected Boron (B) deficiency have increased recently in Kentucky tobacco fields, potentially due to recent changes in management practices. The symptoms observed in the field include; hollow stalk, stunted growth, deformed or no bud formation, small slits on the lower leaf midrib and uncontrollable breaking of the midrib approximately two inches from the stalk. B is a micronutrient tobacco needs in minute amounts, however excessive additions of B could cause toxicity. The objectives of this work were to 1) establish critical points for B sufficiency, 2) describe and define B deficiency and toxicity symptoms and 3) develop …