Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Plant Sciences
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
The Journal of Undergraduate Research
Mutated winter wheat lines were screened for improved drought resistance compared to the wildtype by withholding water for 3 weeks after 9 weeks of normal growth, and noting their responses upon re-watering. Of the 146 mutants grown, 73% were either killed due to water stress or showed poor recovery, while 27% recovered better than the wildtype line. This experiment enabled the selection of candidates for further investigation to better understand the genetic basis of drought tolerance in winter wheat.
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
The Journal of Undergraduate Research
Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures …