Other Life Sciences Commons^™

Amphibian Occupancy And Functional Connectivity Of Restored Wetlands In The Missouri River Floodplain, Michelle L. Hellman

School of Natural Resources: Dissertations, Theses, and Student Research

Wetland decline may threaten many taxa including shorebirds, amphibians, and fish. As agencies increase restoration of wetland habitat, monitoring is crucial to inform the process. Permeable skin sensitive to water quality and biphasic life histories requiring both terrestrial and aquatic habitat make amphibians good indicators of wetland health. I modeled amphibian occupancy in restored Missouri River bends to determine habitat characteristics associated with the presence of amphibians.

Occupancy modeling acknowledges imperfect detection and allows the inclusion of detection covariates. To assess detection I examined two methods currently used to assess anuran occupancy in wetlands, aural anuran surveys and tadpole dip-netting. …

Histone Biotinylation In Candida Albicans, Sahar Hasim, Swetha Tati, Nandakumar Madayiputhiya, Renu Nandakumar, Kenneth W. Nickerson

Kenneth Nickerson Papers

Candida albicans is an opportunistic fungal pathogen in humans. It is a polymorphic fungus: it can live as yeasts, hyphae, or pseudohyphae. Biotin is required for cell growth and fatty acid metabolism because it is used as a cofactor for carboxylases such as acetyl-CoA carboxylase, and pyruvate carboxylase. In addition, we have discovered that biotin is used to modify histones in C. albicans. Biotinylation was detected by Western blots using a monoclonal antibiotin HRP-conjugated antibody as well as with qTOF and LC/MS/MS mass spectrometry. As a precaution, the antibiotin antibody was dialyzed against neutravidin prior to use. During this study, …

Los Hongos Comestibles De La Región De Molango De Escamilla, Hidalgo, México, Miriam Jiménez-González, Leticia Romero-Bautista, Miguel Ángel Villavicencio-Nieto, Blanca Estela Pérez-Escandón

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se realizaron 28 salidas a la región de Molango de Escamilla entre los años 2004-2006, en la temporada de lluvias con el fin de obtener los cuerpos fructíferos de los hongos que son consumidos en la región, así como recopilar los nombres comunes e identificar las especies. Se aplicaron entrevistas estructuradas, semiestructuradas y abiertas. Se identificaron 19 especies y en este trabajo se describen 13. Las especies comestibles mayormente consumidas fueron Pleurotus djamor, Cantharellus odoratus, Armillaria tabescens, Polyporus alveolaris y Amanita tecomate. Se reporta por primera vez en la región el uso comestible de Russula cessans, Russula virescens, Russula aff. …

Candida Albicans Czf1 And Efg1 Coordinate The Response To Farnesol During Quorum Sensing, White-Opaque Thermal Dimorphism, And Cell Death, Melanie L. Langford, Jessica C. Hargarten, Krista D. Patefield, Elizabeth Marta, Jill R. Blankenship, Saranna Fanning, Kenneth W. Nickerson, Audrey L. Atkin

Kenneth Nickerson Papers

Quorum sensing by farnesol in Candida albicans inhibits filamentation and may be directly related to its ability to cause both mucosal and systemic diseases. The Ras1-cyclic AMP signaling pathway is a target for farnesol inhibition. However, a clear understanding of the downstream effectors of the morphological farnesol response has yet to be unraveled. To address this issue, we screened a library for mutants that fail to respond to farnesol. Six mutants were identified, and the czf1/czf1 mutant was selected for further characterization. Czf1 is a transcription factor that regulates filamentation in embedded agar and also whiteto- opaque switching. We found …