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Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Richard W. Beeman, Marce D. Lorenzen, John E. Foster, Blair D. Siegfried
Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Richard W. Beeman, Marce D. Lorenzen, John E. Foster, Blair D. Siegfried
Department of Entomology: Faculty Publications
To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …