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Full-Text Articles in Other Nutrition
The Rna Cargo In Small Extracellular Vesicles From Chicken Eggs Is Bioactive In C57bl/6j Mice And Human Peripheral Blood Mononuclear Cells Ex Vivo, Deborah Fratantonio, Javaria Munir, Jiang Shu, Katherine Howard, Scott R. Baier, Juan Cui, Janos Zempleni
The Rna Cargo In Small Extracellular Vesicles From Chicken Eggs Is Bioactive In C57bl/6j Mice And Human Peripheral Blood Mononuclear Cells Ex Vivo, Deborah Fratantonio, Javaria Munir, Jiang Shu, Katherine Howard, Scott R. Baier, Juan Cui, Janos Zempleni
Department of Nutrition and Health Sciences: Faculty Publications
Small extracellular vesicles (sEVs) and their RNA cargo in milk are bioavailable in humans, pigs, and mice, and their dietary depletion and supplementation elicits phenotypes. Little is known about the content and biological activity of sEVs in foods of animal origin other than milk. Here we tested the hypothesis that sEVs in chicken eggs (Gallus gallus) facilitate the transfer of RNA cargo from an avian species to humans and mice, and their dietary depletion elicits phenotypes. sEVs were purified from raw egg yolk by ultracentrifugation and authenticated by transmission electron microscopy, nano-tracking device, and immunoblots. The miRNA profile …
Small Extracellular Vesicles In Milk Cross The Blood-Brain Barrier In Murine Cerebral Cortex Endothelial Cells And Promote Dendritic Complexity In The Hippocampus And Brain Function In C57bl/6j Mice, Fang Zhou, Pearl Ebea, Ezra Mutai, Haichuan Wang, Sonal Sukreet, Shya Navazesh, Haluk Dogan, Wenhao Li, Juan Cui, Peng Ji, Denise M.O. Ramirez, Janos Zempleni
Small Extracellular Vesicles In Milk Cross The Blood-Brain Barrier In Murine Cerebral Cortex Endothelial Cells And Promote Dendritic Complexity In The Hippocampus And Brain Function In C57bl/6j Mice, Fang Zhou, Pearl Ebea, Ezra Mutai, Haichuan Wang, Sonal Sukreet, Shya Navazesh, Haluk Dogan, Wenhao Li, Juan Cui, Peng Ji, Denise M.O. Ramirez, Janos Zempleni
Department of Nutrition and Health Sciences: Faculty Publications
Human milk contains large amounts of small extracellular vesicles (sEVs) and their microRNA cargos, whereas infant formulas contain only trace amounts of sEVs and microRNAs. We assessed the transport of sEVs across the blood-brain barrier (BBB) and sEV accumulation in distinct regions of the brain in brain endothelial cells and suckling mice. We further assessed sEV-dependent gene expression profiles and effects on the dendritic complexity of hippocampal granule cells and phenotypes of EV depletion in neonate, juvenile and adult mice. The transfer of sEVs across the BBB was assessed by using fluorophore-labeled bovine sEVs in brain endothelial bEnd.3 monolayers and …
Programming Heart Disease: Does Poor Maternal Nutrition Alter Expression Of Cardiac Markers Of Proliferation, Hypertrophy, And Fibrosis In Offspring?, Cathy Chun
Honors Scholar Theses
Maternal malnutrition can affect fetal organogenesis, metabolic processes, and factors involved in developmental regulation. Of the many physiological effects poor maternal nutrition can induce in offspring, one of the most important organs affected is the heart. Cardiovascular disease has been associated with poor maternal diet. It also been suggested that hypertension can originate during impaired intrauterine growth and development. Hypertension can trigger hypertensive heart disease and is associated with numerous heart complications. We hypothesized that poor maternal nutrition would alter critical growth factors associated with normal heart development, specifically, insulin-like growth factor (IGF)-1, IGF-2, transforming growth factor (TGF)β, and connective …
Identification And Assessment Of Markers Of Biotin Status In Healthy Adults, Wei Kay Eng, David Giraud, Vicki L. Schlegel, Dong Wang, Bo Hyun Lee, Janos Zempleni
Identification And Assessment Of Markers Of Biotin Status In Healthy Adults, Wei Kay Eng, David Giraud, Vicki L. Schlegel, Dong Wang, Bo Hyun Lee, Janos Zempleni
Department of Nutrition and Health Sciences: Faculty Publications
Human biotin requirements are unknown and the identification of reliable markers of biotin status is necessary to fill this knowledge gap. Here, we used an outpatient feeding protocol to create states of biotin deficiency, sufficiency and supplementation in sixteen healthy men and women. A total of twenty possible markers of biotin status were assessed, including the abundance of biotinylated carboxylases in lymphocytes, the expression of genes from biotin metabolism and the urinary excretion of biotin and organic acids. Only the abundance of biotinylated 3-methylcrotonyl-CoA carboxylase (holo-MCC) and propionyl-CoA carboxylase (holo-PCC) allowed for distinguishing biotin-deficient and biotin-sufficient individuals. The urinary excretion …