Nutrition Commons^™

Desensitization Of Hacat Keratinocytes To Vitamin D3 Occurs Via Loss Of Redd1 Regulation Of The Mtor Pathway, Michelle Leigh Jones

Wayne State University Theses

Vitamin D3 treatment has long been considered in the treatment of cancer due to its well-documented anti-proliferative effects in vitro. While clinical studies have been positive in other cancers, results are inconclusive in cases of basal cell carcinoma. To better understand the reasons underlying this disconnect, this study employs an immortalized human keratinocyte cell line (HaCaT) to observe the effects of vitamin D3 treatment on cellular proliferation. The results show that both activate vitamin D3 (calcitriol) and its precursor (cholecalciferol) increase proliferation of these cells. Real-time PCR and western blot data indicate that the mTOR pathway becomes activated despite increased …

Detection Of Beneficial Microbiota In Mouse Colon, Aranya Linpisanl

Wayne State University Theses

Lactic acid bacteria (LAB) and Bifidobacterium are the most common types of microbes used as probiotics. They are present in the human gastrointestinal tract and have a significant influence on our health and well-being. Microbiota plays an important role in host metabolism and provides a natural defense mechanism against invading pathogens. This experiment was focusing on establish a method to detect the gastrointestinal tract microbiota, either by fecal or colonic tissue DNA extraction.

The experiment comparing 2 types of DNA extraction; ZR Fecal kit and DNAzol direct. DNAzol direct was easy to use but was not suitable for long term …

Delta-Tocotrienol Downregulates Mmp-9 Expression In Nsclc Cells, Rohini Sri Harshini Pindiprolu

Wayne State University Theses

Lung cancer is the leading cause of cancer death in Unites States. The main types of lung cancer are small cell lung cancer and non small cell lung cancer. NSCLC accounts for about 80% of the cases with less than 15% of patients surviving beyond 5 years. Most common causes of lung cancer are smoking, inhaling asbestos fiber, radon gas, passive smoking, air pollution, familial predisposition, prior history of lung cancer etc. Surgery, chemotherapy, targeted therapy, palliative care are commonly used treatment techniques for lung cancer. Radiation therapy is an important part of lung cancer management. The objective of this …

The Effect Of Dietary Folate On Tissue Folate Status, Ghada Aoun

Wayne State University Theses

ABSTRACT

THE EFFECT OF DIETARY FOLATE ON TISSUE FOLATE STATUS.

by

Ghada Aoun

December 2015

Advisor: Dr. Diane Cabelof

Major: Nutrition & Food Science

Degree: Masters of Science

The folates are a family of structurally similar, water soluble, B vitamins that play a crucial role in human health and disease. Folate has an important role in the synthesis of DNA, DNA methylation, and cellular growth. Folate coenzymes function in accepting and donating one-carbon units in the folate metabolic pathway. Folate deficiency impairs DNA synthesis, induces cancer, and causes abnormalities such as neural tube defects and megaloblastic anemia. While scientific research …

Curcumin Attenuates The Effects Of Atherogenic Diet In Aged Male Brown Norway Rats, Vindhyaja Srirajavatsavai

Wayne State University Theses

ABSTRACT

CURCUMIN ATTENUATES THE EFFECTS OF ATHEROGENIC DIET IN AGED MALE BROWN NORWAY RATS

by

VINDHYAJA SRIRAJAVATSAVAI

DECEMBER 2015

Advisor: Dr. Smiti Gupta

Major: Nutrition and Food Science

Degree: Master of Science

Cardiovascular disease (CVD) is a universal problem in modern society. Hyperlipidemia is a major risk factor for the development of cardiovascular disease. Hyperlipidemia further leads to increased oxidation and inflammation. Curcumin has long been used as a spice and food-coloring agent. In experimental animals, curcumin has shown anti-diabetic, anti-inflammatory, cytotoxic and anti-oxidant properties. In this study, Aged Brown Norway Rats were fed 1 of 3 experimental diets: a …

Potential Antidiabetic And Antioxidant Activitys Of Selected Medicinal Plants, Paba Surangi Sathsara Saranasingha Edirisuriya

Wayne State University Theses

Explore traditionally used medicinal plants for diabetes mellitus would be beneficial to find an alternative option for controlling global diabetic prevalence. Present study aims to compare and identify potential antidiabetic activity in Tinospora cordifolia, Gymnema sylvestre and Aegle marmelos. Fifty percent acetone and ethyl acetate extracts of each plant were studied for anti-diabetic activity using different assays (ORAC, DPPH, ABTS, TPC, α-glucosidase inhibition). Total phenolic content and anti oxidant properties were significantly different among three plants. Tinospora cordifolia ethyl acetate extract possessed significantly higher anti oxidant activity and total phenolic content. DPPH and ABTS results were significantly correlated with each …