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Identification Of Non-Nuclear Receptors For 1,25-Dihydroxyvitamin D3 In Chick Kidney And Brain, Zhiheng Jia

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) has been shown to mediate the rapid, non-nuclear stimulation of calcium and phosphate transport in chick intestine through binding to a receptor localized in the basal lateral membrane. By using an antibody to the N-terminus of the membrane receptor, studies were undertaken to determine whether a comparable protein exists in kidney and brain, and whether it is present in a particular subcellular fraction.

The first step was to establish fractionation protocols to separate subcellular organelles as judged by marker enzyme analyses. Differential centrifugation and Percoll gradient fractions were prepared from chick kidney …