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Full-Text Articles in Laboratory and Basic Science Research
Calcitriol Increases Ceramide, Diacylglycerol, And Expression Of Genes Involved In Lipid Packaging In Skeletal Muscle, Grace Elizabeth Jefferson
Calcitriol Increases Ceramide, Diacylglycerol, And Expression Of Genes Involved In Lipid Packaging In Skeletal Muscle, Grace Elizabeth Jefferson
Theses and Dissertations--Kinesiology and Health Promotion
Background: Vitamin D is crucial for skeletal muscle function. 25-hidroxyvitamin D (25(OH)D) has been correlated with skeletal muscle mass and intramyocellular lipid (IMCL) content. The purpose of this study was to understand how calcitriol, the active vitamin D metabolite, directly affects myocellular size and lipid partitioning.
Methods: C2C12 myotubes were treated with calcitriol (100nM) or vehicle control for 24 or 96 h. Myotube diameter and protein synthesis rate were measured to determine effects of calcitriol on myocellular size. Intramyocellular triacylglycerol (IMTG), diacylglycerol (DAG), and ceramide content were measured by LC/MS. Expression of genes involved in lipid packaging …