Open Access. Powered by Scholars. Published by Universities.®
Laboratory and Basic Science Research Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Alzheimer's disease (1)
- Amyloid beta (1)
- Diabetes (1)
- Differentiation (1)
- Extracellular matrix (1)
-
- Extraembryonic Endoderm (1)
- F9 (1)
- Fibrin (1)
- GLI (1)
- Hedgehog (1)
- Hypoxia (1)
- IHH (1)
- Inflammation (1)
- Integrins (1)
- Islet (1)
- LDHB (1)
- Mitochondrial membrane potential (1)
- Monocarboxylate transport (1)
- Neo-vascularization (1)
- Pancreas (1)
- Primitive Endoderm (1)
- ROS (1)
- Retinoic Acid (1)
- WNT (1)
- Wound healing (1)
Articles 1 - 4 of 4
Full-Text Articles in Laboratory and Basic Science Research
Characterizing Hypoxia, Neutrophil Persistence And Revascularization In The Murine Db/Db Model Of Type Ii Diabetic Impaired Skin Healing, Michael R. Grynyshyn
Characterizing Hypoxia, Neutrophil Persistence And Revascularization In The Murine Db/Db Model Of Type Ii Diabetic Impaired Skin Healing, Michael R. Grynyshyn
Electronic Thesis and Dissertation Repository
Impaired skin healing represents a significant clinical burden. In the diabetic, inflammatory aberrations, hypoxia and insufficient angiogenesis all result in negative wound healing outcomes - repeated infections, poor perfusion and ultimately amputation. Previous research has reported comparable levels of neutrophils in closed wounds up to 4-12 weeks old. Our study interest was in investigating the dynamics of hypoxia resolution, neutrophil persistence and angiogenic response in the db/db model. Contrary to our hypothesis, we observed significantly higher hypoxic load in the wild types at days 3 and 7. Additionally, we observed significantly elevated neutrophil numbers at day 7 db/db wound bed …
Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert
Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert
Electronic Thesis and Dissertation Repository
Mouse F9 cells differentiate into primitive extraembryonic endoderm (PrE) with retinoic acid (RA) treatment, resulting in up-regulation of Gata6, which when translated directly activates Wnt6. Canonical Wnt signalling is required for PrE differentiation, and this, like most developmental processes, requires input from one or more additional pathways, including hedgehog (Hh). The Hh pathway is regulated by GATA6, and crosstalks positively/negatively with Wnt signalling. Ihh up-regulation in F9 cells accompanies PrE induction, but a role for GATA6 or Hh pathway activation in obligatory Wnt/ß-catenin signalling is not known. To address this, I show that RA induces Ihh and altered expression of …
The Role Of Lactate Dehydrogenase B In Aerobic Glycolysis-Mediated Resistance To Ab Toxicity, Tyler Tam
The Role Of Lactate Dehydrogenase B In Aerobic Glycolysis-Mediated Resistance To Ab Toxicity, Tyler Tam
Electronic Thesis and Dissertation Repository
Alzheimer’s disease is a progressive, neurodegenerative disorder characterized by the accumulation of amyloid β (Aβ) plaques in affected brain regions. Strong evidence indicates that Aβ exerts neurotoxic effects by promoting mitochondrial dysfunction and ROS production, leading to widespread oxidative damage and activation of pro-apoptotic mechanisms. Past investigations suggest that neuronal resistance to Aβ toxicity is partly mediated by a Warburg Effect-like metabolism, in which cells exhibit elevated glycolytic activity and lactate production, while limiting mitochondrial respiration. Elevated lactate dehydrogenase A (LDHA) activity, which catalyzes lactate production from pyruvate, has been demonstrated to counter Aβ-induced oxidative stress and neurotoxicity, however the …
The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel
The Role Of Integrins In Support Of Pancreatic Function, Survival And Maturation, Matthew Riopel
Electronic Thesis and Dissertation Repository
The pancreas is a glandular organ composed of endocrine and exocrine compartments. Integrins are cell adhesion molecules that connect cells to the extracellular matrix (ECM). Integrins modulate a variety of cellular effects, yet their mechanism of action in the developed pancreas is not well understood. Fibrin is a provisional ECM protein that contains ligands for integrin receptors. Fibrin is capable of supporting islet health, but it is unclear how fibrin exerts its effects. The objective of this thesis is to understand the role of integrin receptors on in vivo pancreatic cell function, survival, and proliferation. In addition, this thesis investigates …