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Full-Text Articles in Laboratory and Basic Science Research
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
Rajesh Pati
Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Rajesh Pati
Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence …